Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,478,324	0	T	G	44.9%	122.2 / 12.6	70	G33G (GGT→GGG)	ynbB	putative CDP‑diglyceride synthase
Reads supporting (aligned to +/- strand):  ref base T (8/30);  new base G (31/0);  total (40/30)
Fisher's exact test for biased strand distribution p-value = 1.65e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTT  >  NC_000913/1478174‑1478474                                                                                                                                                          |                                                                                                                                                        ggACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGt                                                                                                                                                         <  2:813112/151‑1 (MQ=255)      gCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAgg                                                                                                                                                     <  2:1020651/150‑1 (MQ=255)            aaTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑AGGGGGGAAAAAGGGGGGAGGGTACAtt                                                                                                                                              >  1:1116209/1‑151 (MQ=255)                gTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGGGGGAAAAGGGGGGGGGGGTACa                                                                                                                                                >  1:198097/1‑145 (MQ=255)                gTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGt                                                                                                                                          <  2:829045/151‑1 (MQ=255)                     cGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCg                                                                                                                                     <  2:648637/151‑1 (MQ=255)                      ggAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGGGAGAAAAGGGGGGGAGGGTACATTaaacaattgt                                                                                                                                    >  1:1063334/1‑142 (MQ=255)                       gAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGGGGGGGTACATTACGTAtt                                                                                                                                       >  2:465643/1‑147 (MQ=255)                        aGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CGGAGCGGAAAGGGGGGGAGGGTAAAtttccgatt                                                                                                                                       >  2:904550/1‑139 (MQ=255)                            tgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCtg                                                                                                                              <  1:515192/150‑1 (MQ=255)                                tcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCtggtg                                                                                                                           <  1:479227/149‑1 (MQ=255)                                  ttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGGGAGAAAAGGGGGGGGGGGTACATTACGTATTTG‑TACCTGGTgggg                                                                                                                        >  2:739729/1‑150 (MQ=255)                                  ttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CGGGGAGGAAAAGGGGGGGGGGTACATTACGGATTTG‑TACCTTggggggg                                                                                                                       >  2:858003/1‑150 (MQ=255)                                  ttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CGGGGAGAAAAGGGGGGGGGGGTACATTACGTATTTG‑TACCTGGTggggg                                                                                                                       >  2:311359/1‑150 (MQ=255)                                      gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCg                                                                                                                   <  2:1031013/151‑1 (MQ=255)                                           cTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGATGATAAACCGTTTTCTGCTATGGCGGTT‑T‑A‑CTGAGAGAAAAGGGGGGGAGGTTTAATTAAGTATTCG‑TGCCTGGTgggggtaagttatt                                                                                                              >  1:1150972/1‑141 (MQ=255)                                             gAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCAtt                                                                                                              <  2:954503/149‑1 (MQ=255)                                             gAAATATAATGATGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑CACGAGGGGAAAAGGGGGGGGGGTT‑CATTTCGTATTTC‑TTCCCTGTGGGGGATCGTCAttt                                                                                                             >  1:1145543/1‑150 (MQ=255)                                              aaaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CGGGGGGAAAAGGGGGGGAGGGTATATTACGTTTTTG‑TACCTGGTGGGGGAGCGTCAttt                                                                                                             >  1:140788/1‑149 (MQ=255)                                                 tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑G‑A‑CGGGGGGGAAAGGGGGGGAGGGTTCATTTATTTTTCG‑TATCCTGTGGGGGAtgctgccttttttt                                                                                                        >  1:82140/1‑138 (MQ=255)                                                 tataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CCGAGAGAAAAGGGGGGGAGGGTACATTTCGTATTCG‑GACCCGGTGGGGGATCGTCATTTGtttt                                                                                                        >  1:461627/1‑151 (MQ=255)                                                   taATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CGGGGGGGAAAAGGGGGGGGGTTTCATTTCGTTTTTG‑TTCCTGGGGGGGGCTCGTTATTTGtttttt                                                                                                      >  1:204264/1‑150 (MQ=255)                                                       gCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑ATC‑CGCGGGGAAAAGGGGG‑GAGGATAGATTTAGTTTTCG‑TTCCCGGGGGGGGATTGTCCTTTGttttt                                                                                                       >  1:159777/1‑146 (MQ=255)                                                       gCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATg                                                                                                  <  1:854449/151‑1 (MQ=255)                                                        cTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑T‑A‑CGGAGAGAAAAGGGGGGGGGGGTACATTACTGATTTG‑TTCCTGGTGGGGGATCGTCATTTGtttttttat                                                                                                   >  1:921624/1‑145 (MQ=255)                                                         tGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTGT‑C‑CGGGGGAAAAAGGGGGGGGGGTTGATTTTCG‑ATTTT‑TACCTGGGGGGGCGTCTTTCTTTGttttttcattgt                                                                                                >  2:997158/1‑144 (MQ=255)                                                         tGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑T‑C‑CGGAGAGAAAAGGGGGGGGGGGTACATTACGTATTCG‑TACCTGGTGGGGGATCGTCATTTTTTTTTTAATgg                                                                                                 >  2:256438/1‑150 (MQ=255)                                                           gAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGGGGGAAAAGGGGGGGGGGGTTCATTACGTATTCG‑TCCCTGGGGGGGCATCGTCATTTGttttt                                                                                                       >  2:723642/1‑142 (MQ=255)                                                           gAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGa                                                                                              <  2:381891/151‑1 (MQ=255)                                                           gAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGa                                                                                              <  1:917062/151‑1 (MQ=255)                                                               aaTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑AGGAGGGAAAAAGGGGGGAGGGTTTATTACGACTTGT‑TCCCCGGTGGGGGATGCGCCTTTTTTTTTTTATGGttttttt                                                                                          >  1:897678/1‑145 (MQ=255)                                                                aTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGGGAAAAGGGGGGGGGGTTACATTACGTATTCG‑TACCTGGGGGGGGATCGTCATTTGTTTTTTTATGGTGAttt                                                                                           >  2:905228/1‑149 (MQ=255)                                                                aTCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CGGGGAGAAAAGGGGGGGGGGGTACATTTCGTATTTG‑TACCTGGGGGGGGATCGGCATttttttttttattgggatt                                                                                            >  2:1072172/1‑137 (MQ=255)                                                                 tctcTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGGGAGAAAAAGGGGGGGGGGTACATTTCGTATTTG‑TACCTGGGGGGGGATCGTCATTTGTTTTTTTATGGTGAttt                                                                                           >  1:716607/1‑148 (MQ=255)                                                                   tctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑T‑A‑CGGGGAGAAAAGGGGGGGAGGGTACATTAAGTATTTG‑TAACTGGTGGGGGATCGTCATTTGTTTTTCTATTGTGGTTTTAgg                                                                                       >  2:516306/1‑150 (MQ=255)                                                                   tctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑T‑CGGGGAGAAAAGGGGGGGAGGGTTCATTAACGATTCG‑TACCTGGTGGGGGGTCGTCATTTTTTTTTTTATGGGGAttttaag                                                                                       >  2:175849/1‑146 (MQ=255)                                                                    ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCa                                                                                         <  1:718002/147‑1 (MQ=255)                                                                    ctGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAg                                                                                        <  1:1020519/148‑1 (MQ=255)                                                                     tGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CCGGGGGAAAAAGGGGGGAGGTTACATTACGTTTTTG‑TTCCCGGCGGGGGGTGGGCATTTTTTTTTTTGTGGTGGTTTTAGGGcc                                                                                    >  2:211494/1‑151 (MQ=255)                                                                       gCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CGGGGAGAAAAGGGGGGGGGGGTACATTTCGTATTCG‑TACCTGGTGGGGGATCGTCATTTGTTTTTTAATGGTGATTTTAGGGCCAc                                                                                  >  2:572827/1‑151 (MQ=255)                                                                          acacTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCt                                                                               <  2:1007956/151‑1 (MQ=255)                                                                            acTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑AGGAGAGAAAAGGGGGTGAGGTGTTATTACCGATTCGTTACTTGGGGGGGGA‑CGGTATTTGTTTTTTTATGTGGATTTTAAGTCCACGCTgg                                                                             >  1:589244/1‑151 (MQ=255)                                                                                  cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgac                                                                        <  1:32152/150‑1 (MQ=255)                                                                                           aaTTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGGGGGGAAAGGGGGGGAGGGTACATTATGTATTCG‑TACCTGGGGGGGGGTCGTCATTTGTTTTTTAATGGGGGTTTTAGGTCCCCGCTGGATGACGTTGACGttt                                                              >  2:994549/1‑151 (MQ=255)                                                                                              tttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGGGGGAAAAGGGGGGGGGGGTACATTACGTATTCG‑TACCTGGTGGGGGATCGTCCTTTTTTTTTTTATGGTGGTTTTAGGTCCACGCTGGGTGACGTTGACGtttttt                                                           >  2:376950/1‑151 (MQ=255)                                                                                                  gCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTgcgc                                                       <  2:1076665/151‑1 (MQ=255)                                                                                                      cGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGAt                                                   <  2:29515/151‑1 (MQ=255)                                                                                                       gCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGGGAGAAAAGGGGGGGGGGGTACATTACGTATTCG‑TACCTGGGGGGGGGTCGTCATTTTTTTTTTTATGGTGGTTTTCGGGCCCCGCTGGGTGACGTTGACGTTTTTTTTGCTg                                                     >  2:341330/1‑148 (MQ=255)                                                                                                          gATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAg                                                <  1:256259/150‑1 (MQ=255)                                                                                                          gATAAACCGTTTTCTGCTATGGCGGTT‑A‑A‑CGGCGAGAAAAAGGGGTGAGGGTACATTACCTATTCG‑TACCCTGGGGGGGAGCGTCATTTGTTTTTTAATGGTGATTTTAGGGCCACGCTGGGTGACGTTGACGGTTTTTTTGCTGATa                                                  >  1:494000/1‑148 (MQ=255)                                                                                                            tAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGGGGGGGTACATTACGTATTTG‑TACCTGGGGGGGGGTCGTCATTTTTTTTTTTATGGGGATTTTAGGTCCACGCTGGGTGACGTTTACGGTTTTTTCGGTGGTAAGttt                                             >  2:108149/1‑151 (MQ=255)                                                                                                             aaaCCGTTTTCTGCTATGGCGGTT‑A‑A‑CGGGGAGAAAAGGGGGGGAGGGTACATTACGTATTTG‑TACCTGGGGGGGGGTCGTCCTTTGTTTTTTTATGGGGATTTTAGGTCCACGCTGGGTGACGTTTGCGTTTTTTTCGCTGATAAGtttt                                            >  2:442164/1‑151 (MQ=255)                                                                                                               aCCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCt                                          <  1:457316/151‑1 (MQ=255)                                                                                                                  gTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGc                                       <  1:1106003/151‑1 (MQ=255)                                                                                                                     ttCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCAt                                     <  1:1118364/150‑1 (MQ=255)                                                                                                                         gCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGa                                  <  1:572527/149‑1 (MQ=255)                                                                                                                                 ggTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTg                        >  2:39436/1‑151 (MQ=255)                                                                                                                                    t‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc                     >  2:670634/1‑151 (MQ=255)                                                                                                                                      a‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc                     <  2:459756/150‑1 (MQ=255)                                                                                                                                      a‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg                    <  2:45512/151‑1 (MQ=255)                                                                                                                                          cGGAGAGAAACGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg                    >  1:151067/1‑149 (MQ=255)                                                                                                                                          cGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCt                  <  2:788587/151‑1 (MQ=255)                                                                                                                                           ggAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCtt                 >  1:1119672/1‑151 (MQ=255)                                                                                                                                             agagaAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtat               <  2:198272/151‑1 (MQ=255)                                                                                                                                              gagaAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtata              <  2:250301/151‑1 (MQ=255)                                                                                                                                                 aaaaGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCt           <  2:486508/151‑1 (MQ=255)                                                                                                                                                 aaaaGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCt           >  1:889237/1‑151 (MQ=255)                                                                                                                                                  aaaGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg          >  1:376834/1‑151 (MQ=255)                                                                                                                                                      ggggTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACa      <  2:758694/151‑1 (MQ=255)                                                                                                                                                       gggTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAt     <  1:1017672/151‑1 (MQ=255)                                                                                                                                                        ggTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAtt    <  2:1086377/151‑1 (MQ=255)                                                                                                                                                          tGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACAtttt  >  2:406247/1‑151 (MQ=255)                                                                                                                                                          |                                                                                                                                                        GGACTGCGATTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTT‑A‑C‑CGGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCG‑TACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACATTTT  >  NC_000913/1478174‑1478474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑