Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,578,143	0	A	C	39.2%	124.7 / 31.6	74	L79V (TTA→GTA)	maeB	malic enzyme: putative oxidoreductase/phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base A (40/5);  new base C (0/29);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 8.90e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCC  >  NC_000913/2577995‑2578291                                                                                                                                                           |                                                                                                                                                     ggttCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAAcc                                                                                                                                                    <  1:24795/151‑1 (MQ=255) ggttCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAAc                                                                                                                                                     <  1:37217/150‑1 (MQ=255)       aGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCa                                                                                                                                              <  2:414501/151‑1 (MQ=255)             gcgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCAccgccg                                                                                                                                        >  2:684393/1‑151 (MQ=255)              cgACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAACCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGt                                                                                                                                       <  2:1006633/151‑1 (MQ=255)                aCAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCCCCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTAc                                                                                                                                     <  1:162916/151‑1 (MQ=255)                 cAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTAcc                                                                                                                                    >  2:763079/1‑151 (MQ=255)                    cTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGtt                                                                                                                                 >  2:763459/1‑151 (MQ=255)                      tCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AC‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGtt                                                                                                                                 >  1:605137/1‑149 (MQ=255)                       cAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGtt                                                                                                                                 >  1:615029/1‑148 (MQ=255)                        aaTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTag                                                                                                                               >  1:817278/1‑149 (MQ=255)                         aTAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAACCCCGGCGAATTTCTT‑AAACAGACCGCCCTTGCCTTCCACCCCCGT‑TTTG‑CCTGCCAGCGCGCA‑AA‑‑TGTTGCCTACCCCCAGCACCGCCGTACCGTTAgaga                                                                                                                            <  2:379705/151‑1 (MQ=255)                            aaTTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATc                                                                                                                          >  1:1083600/1‑150 (MQ=255)                                          gAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGtt                                                                                                            >  1:625295/1‑150 (MQ=255)                                             ttCGTCACCTTAATTATAAAATACATCACCCCCGGCAATTTTTTA‑AAACAGACCCCCCTTCCCTCCCACCCCGGT‑TTTG‑CCTGCCGGCGCCCA‑AT‑‑TTTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTa                                                                                                           <  2:1040768/148‑1 (MQ=255)                                                  cAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGc                                                                                                   >  2:736936/1‑151 (MQ=255)                                                   accTTAAATGTAAAATACATCACCCCCGGCAAATTTTTA‑AAACAGACCCCCTTTCCCTCCCACCCCCGT‑TTTG‑CCTGCCAGCGCGCA‑AA‑‑TTTTGCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCt                                                                                                        <  2:377873/143‑1 (MQ=255)                                                    aCTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCgg                                                                                                 >  1:805455/1‑151 (MQ=255)                                                      ttCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGt                                                                                                >  2:317201/1‑150 (MQ=255)                                                       tCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGtat                                                                                              >  2:418411/1‑151 (MQ=255)                                                           tGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAttt                                                                                          >  1:1120238/1‑151 (MQ=255)                                                           tGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAttt                                                                                          >  1:323369/1‑151 (MQ=255)                                                            gtaaaaTACACCAACCCCGGCAAATTTCTA‑AAACAGACCGCCCTTCCCTCCCATCCCCGG‑TTTG‑CCTGCCAGCGCCCC‑AA‑‑TGTTGCCAACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg                                                                                         <  2:136734/148‑1 (MQ=255)                                                               aaataccccacccccGGCGAATTTCTA‑AAAAAGACCGCCTTCCCCTCCCCCCACCGT‑TTTG‑CCTGCCGGCCCGCC‑AT‑‑TTTTGCCTACCCCCAGCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg                                                                                         <  1:317012/136‑1 (MQ=255)                                                               aaaaacaccacccccGGCAATTTTCTA‑AAACAGACCCCCCTTCCCTCCCCTCCCCGG‑TTGC‑CCGCCCAGCGCCCC‑AA‑‑GGTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAg                                                                                      <  1:855533/139‑1 (MQ=255)                                                               aaaTACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGt                                                                                        >  1:443504/1‑149 (MQ=255)                                                               aaaTACACCACCCCCGGCGATTTTCTA‑AAACAGACCCCCCTTCCCTCCCACCCCCGT‑TTTG‑CCTGCCGGCGCGCA‑AT‑‑TGTTCCCTACCCCAAGCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAg                                                                                      <  1:826360/151‑1 (MQ=255)                                                               aaaTACACAAACCCCGGCAAATTTCTA‑AAACAGACCGCCTTTCCCTCCCACCCCCGT‑TTTG‑CCTCCCAGCGCCCC‑AA‑‑TGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTg                                                                                         <  2:768523/148‑1 (MQ=255)                                                                 atccaccAACCCCGCCGATTTTCAT‑AACCAGACCCCCCTTGCCTCCCATCACCGG‑TTTG‑CATGCCACCCCGCC‑AT‑‑TGTCGCCAACCCCAAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGt                                                                                        <  1:162837/141‑1 (MQ=255)                                                                   ccaacAACCCCGGCTATTTTCTA‑ACACAGACCGCCCTTCCCTCCCCCCCCGGT‑TTTC‑CCTCCCAGCCCCCC‑AT‑‑TGTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATAttt                                                                                          <  1:762737/139‑1 (MQ=255)                                                                        aaccccGGCAATTTTCTT‑AAACAAAACCCCTTTCCCTCCCATCCCCGT‑TTTG‑CCTCCCAGCGCGCA‑AA‑‑TGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCtttt                                                                                <  2:131213/145‑1 (MQ=255)                                                                        aaTCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAAc                                                                             >  1:893607/1‑151 (MQ=255)                                                                         aTCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACg                                                                            >  1:41741/1‑151 (MQ=255)                                                                           cccGGCAATTTTCTT‑AAACAGACCCCCTTTGCCTCCCACCCCCGT‑TTTC‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACg                                                                            <  2:908944/149‑1 (MQ=255)                                                                           cccGGCAATTTTCTA‑AAACAACACCCCCTTGCCTTCCCCCGCGGT‑TTTG‑CCGCCCAGCGCGCC‑AA‑‑TTTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACgg                                                                           <  1:660069/150‑1 (MQ=255)                                                                            ccGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGt                                                                         >  2:456064/1‑151 (MQ=255)                                                                             cGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTc                                                                        >  2:590061/1‑151 (MQ=255)                                                                               gCGATTTTCTA‑AAACAGACCCCCCTTCCCTCCCACCCCCGT‑TTTC‑CCGGCCGGCGCCCA‑AT‑‑TTTTCCCTACCCCCACCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtt                                                                      <  2:419100/151‑1 (MQ=255)                                                                                  aaTTTTTTAAAACAGACCCCCCTTCCCTCCCCCCCCCGG‑TTTC‑CCGGCAAGCGCCCA‑AT‑‑TGTGCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCtttt                                                                    <  1:516113/151‑1 (MQ=255)                                                                                  aaTTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCttttt                                                                   >  1:31579/1‑151 (MQ=255)                                                                                  aaTTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCttttt                                                                   >  1:131029/1‑151 (MQ=255)                                                                                     ttCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCg                                                                 >  1:651022/1‑150 (MQ=255)                                                                                      tCTA‑AACCAGACCCCCCTTG‑CTTCCCACCCCGG‑TTTGCCCGGCCGGCCCGCC‑AG‑‑TGTCCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGa                                                                <  2:597023/150‑1 (MQ=255)                                                                                             aaaaaaCCCCCCTGCCTTCCCCCCCCCG‑TTTG‑TCCTCC‑GCGCGCC‑AAACTTTTCCCTACCCCGCGCTCCGCCGTACCGTTAGAGATCACCGCCACCAGTTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt                                                             <  1:736601/144‑1 (MQ=255)                                                                                              cAGAAAGCCCTTGCCTTCCATCACCGG‑TCTG‑CCTGCCAGCCAGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTCCCGTTAGAGATCACCGCCACCAGGTTACATCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGAttt                                                             >  1:402245/1‑146 (MQ=255)                                                                                                 accGCCCTTCCCTCCCACCCCCGT‑TTTG‑CCTGCCGGCGCCCA‑AA‑‑TGTCCCCTACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACa                                                      <  1:373479/148‑1 (MQ=255)                                                                                                  acccccTTTCCCTCCCACCCCGGTTTTG‑CCGCCCAGCGCCCT‑AA‑‑TGTTCCCTACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACaa                                                     <  2:356029/148‑1 (MQ=255)                                                                                                    gCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAgg                                                   >  2:1065258/1‑150 (MQ=255)                                                                                                       cctGCCCTCCCTCCCCGG‑TTTG‑CCCGGCCGCGCCCCAAA‑‑GGTTCCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGc                                                <  1:823823/149‑1 (MQ=255)                                                                                                         tcccctccaccgctgg‑TTTC‑CTTGCCGGCGCCCC‑AT‑‑TTTGGCCTACCCCCCGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGc                                                <  1:1092097/134‑1 (MQ=255)                                                                                                           ccTCCCACCACCGT‑TTTG‑CCTCCCAGCGCGCA‑AA‑‑TTTTGCCAACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCgg                                              <  2:1084022/148‑1 (MQ=255)                                                                                                            cTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAAc                                          >  1:246458/1‑151 (MQ=255)                                                                                                            cTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAAc                                          >  1:613276/1‑151 (MQ=255)                                                                                                            cTTCCACCCCCGG‑TTTC‑CCTGCCACCGCCCC‑AT‑‑TTTTCCCTACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTg                                                 <  1:19208/144‑1 (MQ=255)                                                                                                               ccATCCCCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTACCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGcc                                       <  1:1126442/151‑1 (MQ=255)                                                                                                                 aTCACCGG‑TTTG‑CCTGACAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGACACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTg                                     >  1:940711/1‑151 (MQ=255)                                                                                                                    cccGT‑TTTG‑CCTGCCGGCGCGCC‑AA‑‑TGTTCCCTACCCCCAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTg                                  <  2:199244/150‑1 (MQ=255)                                                                                                                     ccGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTg                                  >  2:342150/1‑150 (MQ=255)                                                                                                                       gg‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGt                               >  2:275110/1‑151 (MQ=255)                                                                                                                          tttG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGt                               >  2:1084803/1‑149 (MQ=255)                                                                                                                             g‑cctgccAGCGCGAC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGcc                          >  1:660658/1‑151 (MQ=255)                                                                                                                               cctgccAGCGCACA‑AA‑‑TGTTCCTTACCCCAAGCCCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAgg                            <  1:915084/148‑1 (MQ=255)                                                                                                                                  gccAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAgcg                      >  2:1059976/1‑151 (MQ=255)                                                                                                                                    cAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGcc                    >  2:874767/1‑151 (MQ=255)                                                                                                                                     aGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGcc                    >  2:164414/1‑150 (MQ=255)                                                                                                                                         cgcC‑AA‑‑TTTTCCCAACCCCCGGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGAt                <  2:1053400/150‑1 (MQ=255)                                                                                                                                         ccac‑AA‑‑TGTTGCCTACCCCCACCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGc                           <  1:239950/136‑1 (MQ=255)                                                                                                                                           cc‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgc             >  1:199571/1‑151 (MQ=255)                                                                                                                                            c‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATcgcg            >  2:226356/1‑151 (MQ=255)                                                                                                                                              aa‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCgcgc           >  1:861919/1‑151 (MQ=255)                                                                                                                                              aa‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCgcgc           >  2:660686/1‑151 (MQ=255)                                                                                                                                               a‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTCTATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGGGAGTAGGCCAGCGCCAGATCgcgc           >  1:712714/1‑150 (MQ=255)                                                                                                                                                      cccTACCCCAGGCGCCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGtt     <  2:965580/150‑1 (MQ=255)                                                                                                                                                         tAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGcc  >  1:1155029/1‑151 (MQ=255)                                                                                                                                                          aaCCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGcc  >  1:1137199/1‑150 (MQ=255)                                                                                                                                                           |                                                                                                                                                     GGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTT‑AAACAGAACGCCCTTGCCTTCCATCACCGG‑TTTG‑CCTGCCAGCGCGCC‑AA‑‑TGTTGCCTAACCCCAGCACCGCCGTACCGTTAGAGATCACCGCCACCAGGTTACCTCGGGCGGTATATTTGTAGGCTTTTAACGGGTCTTTTTCGATTTCAAGACAAGGTGCGGCAACGCCTGGTGAGTAGGCCAGCGCCAGATCGCGCTGTGTTGCC  >  NC_000913/2577995‑2578291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑