Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	39.1%	109.8 / 12.5	64	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (6/33);  new base G (25/0);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 1.84e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354138‑354434                                                                                                                                                        |                                                                                                                                                     aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGt                                                                                                                                                      <  2:140109/151‑1 (MQ=255)        cATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCGCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGa                                                                                                                                                <  1:765913/150‑1 (MQ=255)             gTGATGCTGAAGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGTCTGGGGACGCGCGCGATTGAAGAGAGTATCTCCCGCCATACGGGCGTTGCCGAAGTGGCGGC‑GGAGGGGTTGAAAGACgcc                                                                                                                                            >  1:167135/1‑148 (MQ=255)             gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCt                                                                                                                                          <  1:489931/151‑1 (MQ=255)               gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTTGGGGGGaaaatggggtgg                                                                                                                                         >  1:534605/1‑142 (MQ=255)               gACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTg                                                                                                                                         <  2:543155/150‑1 (MQ=255)                aCGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGaa                                                                                                                                       <  2:324640/151‑1 (MQ=255)                 cGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGGAGGGCTGaa                                                                                                                                       >  2:364053/1‑150 (MQ=255)                     gACGGTTATCGCTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAgg                                                                                                                                    <  2:167302/149‑1 (MQ=255)                      aCGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCa                                                                                                                                 <  1:1137932/151‑1 (MQ=255)                         gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑gg                                                                                                                              <  1:824744/150‑1 (MQ=255)                          ttATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑ggt                                                                                                                             <  1:777234/150‑1 (MQ=255)                            aTCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑ggtgg                                                                                                                           <  2:18616/150‑1 (MQ=255)                              cACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑ggtggcg                                                                                                                         <  2:797411/150‑1 (MQ=255)                               aCTTTATTCTCGGGCCCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGCGTATATCCAGCCATCCGGGCGTTGCCGAAGTGGCGGA‑TCGCGGGGGGGAAGAACAGGTGGAAAGGCA‑ggtggcgg                                                                                                                        >  1:494440/1‑150 (MQ=255)                                  ttATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑ggtggcggtg                                                                                                                      <  1:1041343/149‑1 (MQ=255)                                   tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑ggtggcggtggc                                                                                                                    <  1:1014977/150‑1 (MQ=255)                                   tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACCCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGCTGGGGGGGACGATGGGGCGGAAGGGGA‑gggggcgg                                                                                                                        >  1:40997/1‑146 (MQ=255)                                       ctcGGGCGCATTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGCTGAAAGGGGA‑gggggcggggggggtt                                                                                                                >  2:1046708/1‑145 (MQ=255)                                          gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCa                                                                                                            <  1:1114080/151‑1 (MQ=255)                                             cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCAttt                                                                                                         <  2:1133754/151‑2 (MQ=255)                                              gcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGAGTTTGTCATTcc                                                                                                        <  1:816490/151‑1 (MQ=255)                                               cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCg                                                                                                       <  1:734673/151‑1 (MQ=255)                                               cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCg                                                                                                       <  2:425937/151‑1 (MQ=255)                                               cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGAAAGATGCGCTGGAAGGGGA‑GGGGGGGGGGGGGGTTGTCATTcc                                                                                                        >  2:679923/1‑150 (MQ=255)                                               cACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGA‑GGGTGGGGGGAAAGAAGGCCTGAAAGGGGA‑GGGGGCGGGGGGGTTTGTCATTcc                                                                                                        >  2:245632/1‑150 (MQ=255)                                                  tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGGGCTGAAAGGGCA‑GGGGGCGGGGGCGGTTGTCATTCCGaa                                                                                                     >  2:411341/1‑150 (MQ=255)                                                   gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGA‑GGGTGGGGGGGAAGAAGCGCTGGAAGGGGC‑GGGGGGGGGGGCGGTTGTTATTca                                                                                                        >  1:753975/1‑145 (MQ=255)                                                    aCGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGGA‑GGGGGGGGGGGCGGTTGTCCTTCCGgaa                                                                                                    >  2:278009/1‑149 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGGA‑GGGGGCGGGGGCGTTTTTCATTTCGAAAGAGAGc                                                                                              >  2:1015537/1‑150 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGa                                                                                            <  1:815391/150‑1 (MQ=255)                                                              tAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGG‑GGGTGGGGGGGAAGATGGGCTGAAAGGGCA‑GGGGGCGGGGGCGGTTGTCATTCCGCAAGAGAGGGACa                                                                                          >  1:404561/1‑149 (MQ=255)                                                                aCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCt                                                                                      <  2:963222/151‑1 (MQ=255)                                                                 cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTg                                                                                     <  2:1090297/151‑1 (MQ=255)                                                                    tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGa                                                                                   <  2:814801/150‑1 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGGGCTGAAAGGGGA‑GGGGGGGGGGGCGGTTGTCATTCCGGAAGAGAGCGACAGTCTgg                                                                                    >  2:615495/1‑146 (MQ=255)                                                                         ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                             <  1:540724/151‑1 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCA‑GGGGGCGGGGGGGTTTGTCATTCCCAAAGAGAGCGACAGTCTGGAAGATCGTGAtgt                                                                       >  2:946051/1‑151 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGAAGCCATGGAAGGGGAGGGGGGGGGGGGGGTTTGTC‑TTCCGAAAGAGAGGGACCGGCTGGGAGGACGTg                                                                           >  1:882285/1‑147 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCA‑GGGGGGGGGGGCGGTTGTCATTCCGAAAGAGAGGGACAGTCTGGAAGATCGTggt                                                                         >  2:984758/1‑147 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATGCGCTGAAAGGGCA‑GGGGGCGGGGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGGAGATCGTGAt                                                                         >  2:620929/1‑149 (MQ=255)                                                                                   ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                                     <  1:683396/149‑1 (MQ=255)                                                                                   ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGgcg                                                                   <  1:389959/151‑1 (MQ=255)                                                                                     ggACTCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGACGTGGCGGT‑GGGGGGGGGGAAAAATGCGCTGATAGGGCC‑CGTGGGGGGGGGGCTTGCCAATTCGGAAGAGAGCGGACGGCTGGAAGATCCGGATGGGgcgcc                                                                 >  1:804153/1‑150 (MQ=255)                                                                                     ggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCC‑GGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGAAGATCGTGGTGTGgggc                                                                  >  2:47793/1‑150 (MQ=255)                                                                                         gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCg                                                             <  1:940936/151‑1 (MQ=255)                                                                                         gcgcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCA‑GGGGGCGGGGGCGTTTGTCATTTCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGGGCACTc                                                              >  2:15569/1‑150 (MQ=255)                                                                                            cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGTGAAAAAAGCGCTGAAAGGGCA‑GGGGGGGGGGGGGGTTGTCATTTCGAAAGAAAGCGACAGGCTGGAAGATCGTGAAGTGGCGCACTc                                                              >  1:940377/1‑147 (MQ=255)                                                                                             gagaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCA‑GGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑aa                                                         >  2:901016/1‑150 (MQ=255)                                                                                              agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑Aaga                                                       <  2:134072/151‑1 (MQ=255)                                                                                              agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGGAAGATGCGCTGAAAGGGCA‑GGGGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑a                                                          >  2:766049/1‑148 (MQ=255)                                                                                                   gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGGTGGGGGGGAAGATGCGCTGAAAGGGCA‑GGGGGGGGGGGCGGTTGTTATTCCGAAAGAGAGGGACAGGCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGaa                                                    >  1:955497/1‑149 (MQ=255)                                                                                                          gTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGGGAAAGATGCGCTGAAAGGGCA‑GGGGGCGGGGGCGGTTGTCATTCCGAAAGAGAGCGACAGGCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGAtt                                             >  2:1154708/1‑149 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATgg                                         <  1:780815/147‑1 (MQ=255)                                                                                                                 cAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCCGAAAGGGCA‑GGGGGGGGGGGGGTTTGTCATTTCGCAAGAGAGCGACAGTCTGGAAGATCGGGGTGTGGGGCACTCGC‑AAGAGAAGGGGATTATGgggc                                      >  1:76140/1‑149 (MQ=255)                                                                                                                            gCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATGGCGCTGGTGGACAGCCa                         <  1:910860/151‑1 (MQ=255)                                                                                                                             cGTTGCCGAAGTGGCGGT‑GGGTGGGGGGAAAGATACGCTGAAAGGGCC‑CGGGGCGGGGGGGGTTTTCCTTTCCAAAAAGAGCGACAGTCTGGGAGATCGGGGTGGGGGGCACTCGCAAAGGGAAGG‑GACTTTGGCGCcggtgg                                >  1:1040285/1‑143 (MQ=255)                                                                                                                               ttGCCGAAGTGGCGGG‑GGGTTGGGTGAAAGATGCGCTGAAAGGGCA‑GGGGGCGGGGGGGGTTGTCATTTCGAAAGAGAGCGGCAGTCTGGGAGATCGGGATGTGGGGCCCTCGC‑CAGAGAAGGCGATTATGGCGCTGGGGGGCAGccc                         >  1:396469/1‑147 (MQ=255)                                                                                                                                  ccGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTgg                   <  2:752779/151‑1 (MQ=255)                                                                                                                                  ccGAAGTGGCGGG‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCC‑GGTGGCGGTGGCGGTTGTCATTCCGGAAGAGAGGGACAGGCTGGGAGATCGTGATGTGGCGCACTCGC‑AAGAGAAAGCGAATATGGCGCTGGGGGACAGCCAGAtt                     >  1:242841/1‑149 (MQ=255)                                                                                                                                         ggCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACtt             <  2:494728/150‑1 (MQ=255)                                                                                                                                          gCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTg           <  1:71144/151‑1 (MQ=255)                                                                                                                                                ggtTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgccg       <  1:1033556/150‑1 (MQ=255)                                                                                                                                                  ttGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGccc    >  1:659630/1‑151 (MQ=255)                                                                                                                                                    ggggTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCgg  <  1:329258/151‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                     ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGT‑GGTTGGGGTGAAAGATGCGCTGAAAGGGCA‑GGTGGCGGTGGCGTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGC‑AAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCCGCCCGG  >  NC_000913/354138‑354434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑