Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,818	A→G	intergenic (‑72/+251)	kgtP ← / ← rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,818	0	A	G	100.0%	410.8 / NA	99	intergenic (‑72/+251)	kgtP/rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/52);  total (47/52)

CAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAA  >  NC_000913/2725674‑2725965                                                                                                                                                   |                                                                                                                                                    caATCGCCCAAATGCGGCGACAAGAATCACTCCTTGTCAGTTTGGTGTCTGCCAATAATGCAATAATCA‑ACACTCCATGATATCTCCTGACGTAAGAAGATGCTTTTAGCGGTAGCTTTTGTTTA‑TTTTTTTTGTAACGGAACTGTtata                                                                                                                                                <  1:976991/150‑1 (MQ=255) caATCGACCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATAc                                                                                                                                               >  1:957750/1‑151 (MQ=255)   aTCGCCCAAATGCGGCGACGAGTATCCCTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACAt                                                                                                                                             <  2:1144005/151‑1 (MQ=255)   aTCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACAt                                                                                                                                             >  1:436278/1‑151 (MQ=255)   aTCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACAt                                                                                                                                             <  2:48797/151‑1 (MQ=255)     cGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTTCT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACAttt                                                                                                                                           <  1:583697/151‑1 (MQ=255)      gCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATttg                                                                                                                                          >  2:577386/1‑151 (MQ=255)        ccAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATttgtt                                                                                                                                        <  2:588338/151‑1 (MQ=255)        ccAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATttgtt                                                                                                                                        >  1:414970/1‑151 (MQ=255)          aaaTGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATttgttg                                                                                                                                       >  1:269735/1‑150 (MQ=255)            aTGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCat                                                                                                                                    >  1:122110/1‑151 (MQ=255)               cggcgACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATc                                                                                                                                 <  2:1060097/151‑1 (MQ=255)               cggcgACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATc                                                                                                                                 >  1:6357/1‑151 (MQ=255)               cggcgACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATc                                                                                                                                 <  2:724476/151‑1 (MQ=255)                 gcgACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCAt                                                                                                                               <  2:348284/151‑1 (MQ=255)                   gacgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTTCT‑TTCAGCCATGCCTTTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCAt                                                                                                                               <  1:47480/149‑1 (MQ=255)                   gacgaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTa                                                                                                                             <  2:1072784/151‑1 (MQ=255)                      gaGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGc                                                                                                                          >  2:130514/1‑151 (MQ=255)                       aataccACTACTTGTCAGTTTGCTTTCTGCCGTTTGAGTGCT‑TTCGGCCATGCCATTATGTTTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCa                                                                                                                         <  1:1122254/146‑1 (MQ=255)                        gTATCGCTACTTTTCAGTTTGCTGTCTGCCGTTACAGTCCT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGTTAGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCaa                                                                                                                        <  1:244198/151‑1 (MQ=255)                        gTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCaa                                                                                                                        <  1:1005771/151‑1 (MQ=255)                         tATCACTACTTGTCTATTTGCTGTCTGCCGTTACAATACT‑TTCAGCCATGCCATTATTTTTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTAt                                                                                                                            <  1:401104/146‑1 (MQ=255)                            cACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCt                                                                                                                     <  2:600629/150‑1 (MQ=255)                            cACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCt                                                                                                                     <  2:335587/150‑1 (MQ=255)                            cACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCt                                                                                                                     <  1:78659/150‑1 (MQ=255)                              ctactTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTa                                                                                                                   <  2:430157/150‑1 (MQ=255)                               tactTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAAc                                                                                                                 >  1:1072302/1‑151 (MQ=255)                                 ctTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAAcc                                                                                                                >  2:303236/1‑150 (MQ=255)                                  ttGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTaa                                                                                                                  <  1:763488/147‑1 (MQ=255)                                  ttGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCAt                                                                                                              >  2:48641/1‑151 (MQ=255)                                   tGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCa                                                                                                               <  1:233309/149‑1 (MQ=255)                                   tGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCAt                                                                                                              <  2:724283/150‑1 (MQ=255)                                   tGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATg                                                                                                             >  1:428416/1‑151 (MQ=255)                                         tttGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATTTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAAtt                                                                                                         <  1:246493/149‑1 (MQ=255)                                                tCTGCCTTTACAGTACT‑TTCAGCCATGCCATTATGTTTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagtta                                                                                                   <  1:172883/148‑1 (MQ=255)                                                 cTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGc                                                                                                 >  1:401837/1‑149 (MQ=255)                                                 cTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGc                                                                                                 >  1:958477/1‑149 (MQ=255)                                                  tGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAgg                                                                                              >  2:939406/1‑151 (MQ=255)                                                   gCCGTTACAGTTCT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAgg                                                                                              <  1:36550/150‑1 (MQ=255)                                                   gCCGTTACAGTCCT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAg                                                                                               <  1:1051145/149‑1 (MQ=255)                                                   gCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGa                                                                                             <  1:591767/151‑1 (MQ=255)                                                     cGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGa                                                                                             >  1:1116289/1‑150 (MQ=255)                                                       ttACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAAt                                                                                         >  1:637485/1‑151 (MQ=255)                                                        tACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg                                                                                        >  2:562386/1‑151 (MQ=255)                                                              aCT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAtt                                                                                  >  1:864124/1‑151 (MQ=255)                                                               cT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAtt                                                                                  <  2:178373/150‑1 (MQ=255)                                                                  ttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                 >  1:656561/1‑149 (MQ=255)                                                                    cAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgag                                                                               <  1:577086/149‑1 (MQ=255)                                                                     aGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgaggag                                                                            <  2:1115515/151‑1 (MQ=255)                                                                      gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGc                                                                           <  2:754466/151‑1 (MQ=255)                                                                      gccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGc                                                                           >  1:864892/1‑151 (MQ=255)                                                                       ccatgccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCCGGAAAATGGTTATTGAGGAGca                                                                          <  1:211297/151‑2 (MQ=255)                                                                          tgccatTATTTCTCCTGTCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTa                                                                        <  1:883182/150‑1 (MQ=255)                                                                           gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTa                                                                        >  1:286123/1‑149 (MQ=255)                                                                              atTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGAt                                                                   >  2:125907/1‑151 (MQ=255)                                                                               ttATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAGCCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGa                                                                    <  1:655458/149‑1 (MQ=255)                                                                               ttATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGAt                                                                   <  1:440528/150‑1 (MQ=255)                                                                                tATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATa                                                                  <  2:743429/150‑1 (MQ=255)                                                                                tATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATa                                                                  <  1:257699/150‑1 (MQ=255)                                                                                      tcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                           >  2:352614/1‑151 (MQ=255)                                                                                       ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                          <  1:697224/151‑1 (MQ=255)                                                                                       ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                          <  1:383766/151‑1 (MQ=255)                                                                                       ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTg                                                          >  2:116938/1‑151 (MQ=255)                                                                                          gCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTgg                                                         <  2:17595/149‑1 (MQ=255)                                                                                            cGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTa                                                       >  2:1097499/1‑149 (MQ=255)                                                                                            cGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTa                                                       >  2:610757/1‑149 (MQ=255)                                                                                               aaTCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAagg                                                    >  2:917048/1‑149 (MQ=255)                                                                                                aTCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAagga                                                   <  1:1086832/149‑1 (MQ=255)                                                                                                  ccGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGac                                               <  1:1124759/151‑1 (MQ=255)                                                                                                   cGATGCTTTTGCCCGTCGCTTTTGTTTG‑TTTTTTTCGGAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCCTGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTCAGGATAAATTTCTGGTAAggagga                                                >  1:983624/1‑149 (MQ=255)                                                                                                     aTGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACg                                            <  1:937369/151‑1 (MQ=255)                                                                                                     aTGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACg                                            >  1:760649/1‑151 (MQ=255)                                                                                                      tGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGt                                           >  1:263681/1‑151 (MQ=255)                                                                                                       gCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCTGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGt                                           >  1:106696/1‑150 (MQ=255)                                                                                                             gtcggtcgCTTTTGTTTA‑TTTCTTTTGAAAAGGAAATGTTATACATTTGCTGCATATCATTATGCAACCTTAACCATGACTTTAGTTAGCAGGAAAATGGATATTGCGGAGCTTAAGGATGAATTTCTGGTAAGGAGGATACGTATGGa                                      >  1:250303/1‑149 (MQ=255)                                                                                                              tcggtcgCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                   >  1:216004/1‑151 (MQ=255)                                                                                                                ggtcgCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTgg                                 >  1:802169/1‑151 (MQ=255)                                                                                                                 gtcgCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTgg                                 <  1:966892/150‑1 (MQ=255)                                                                                                                  tcgCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGc                               >  2:813118/1‑151 (MQ=255)                                                                                                                   cgCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCa                              <  2:428663/151‑1 (MQ=255)                                                                                                                    gCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCa                              >  2:836793/1‑150 (MQ=255)                                                                                                                    gCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCa                              >  2:556899/1‑150 (MQ=255)                                                                                                                    gCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCa                              >  1:633918/1‑150 (MQ=255)                                                                                                                         tGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTgg                        >  2:118386/1‑151 (MQ=255)                                                                                                                         tGTTTA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTg                         <  2:691728/150‑1 (MQ=255)                                                                                                                            ttA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTgggg                      <  2:1127924/150‑1 (MQ=255)                                                                                                                             tA‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGa                     <  1:918680/150‑1 (MQ=255)                                                                                                                              a‑TTTTTTTTGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGa                     >  1:309498/1‑149 (MQ=255)                                                                                                                                 tttttttGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGACAAGTTGGGGAAGcc                 >  1:799567/1‑151 (MQ=255)                                                                                                                                       tGTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATc            <  1:318841/150‑1 (MQ=255)                                                                                                                                        gTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATcc           <  2:122259/150‑1 (MQ=255)                                                                                                                                        gTAAAGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCg          >  2:986978/1‑151 (MQ=255)                                                                                                                                          aaaGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGtt        >  1:883752/1‑151 (MQ=255)                                                                                                                                          aaaGGAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGtt        <  1:94810/151‑1 (MQ=255)                                                                                                                                              gAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTg    >  2:684890/1‑151 (MQ=255)                                                                                                                                              gAAATGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTg    >  1:274753/1‑151 (MQ=255)                                                                                                                                               aaaTGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGa   <  2:954909/151‑1 (MQ=255)                                                                                                                                                aaTGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGaa  <  1:48622/151‑1 (MQ=255)                                                                                                                                                aaTGTTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGa   >  1:368756/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CAATCGCCCAAATGCGGCGACGAGTATCACTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACT‑TTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTA‑TTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAA  >  NC_000913/2725674‑2725965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑