| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 702,338 | (T)7→8 | coding (414/1149 nt) | nagA ← | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 702,331 | 1 | . | T | 97.7% | 162.7 / ‑4.4 | 44 | coding (421/1149 nt) | nagA | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (22/21); total (22/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑C‑‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGT > NC_000913/702185‑702475 | aaTCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑C‑‑tttt < 1:616937/151‑1 (MQ=255) aTTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAg < 2:585284/151‑1 (MQ=255) tttGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGcc > 2:64472/1‑150 (MQ=255) tgatgaCTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGAc > 2:682696/1‑151 (MQ=255) gccGCAGGAACCATTCCCGGTGCCAGGGTCTCTTTGGTAATGACGTCGGCGTTTTCCCACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAg < 2:283823/149‑1 (MQ=255) ccGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGa < 1:259646/150‑1 (MQ=255) cAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGc > 1:592294/1‑150 (MQ=255) aGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGc > 2:383646/1‑149 (MQ=255) ccGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGcc < 1:165589/150‑1 (MQ=255) ggTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGa < 1:301307/151‑1 (MQ=255) ttaCTTTGGTAATGACGTCGGCGCTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGCGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGCCCTAACGCCTGATTCGGATGtt > 2:289548/3‑151 (MQ=255) tCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGtt > 1:252075/1‑151 (MQ=255) aCTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGtttt < 2:231006/151‑1 (MQ=255) ggTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGcc > 1:482520/1‑149 (MQ=255) tAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGt > 2:79958/1‑151 (MQ=255) cGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTcgc > 2:253225/1‑151 (MQ=255) gTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTcgcg < 1:64481/151‑1 (MQ=255) tCGGTGTTTTCAAACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAACTTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCgcgc > 2:444902/1‑151 (MQ=255) tCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCgcgc > 1:42477/1‑151 (MQ=255) tCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAAcgcg < 1:242745/150‑1 (MQ=255) cacaGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACg > 1:285528/1‑151 (MQ=255) caGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGcc > 2:293854/1‑151 (MQ=255) aaTCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGtt < 2:619713/151‑1 (MQ=255) aaTCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGtt < 2:337800/151‑1 (MQ=255) ccAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcatca < 1:697583/151‑1 (MQ=255) cAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTtcatc > 2:52230/1‑149 (MQ=255) aGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGc < 2:657489/151‑1 (MQ=255) cgcgGCATCAGGCTTACGCACACTATCCGGATTATGGGTGCTT‑‑TTTTTTTTCCAGATTCAGCCGCGGCCCTTCCTGATGCAGTCCTAACGCCTGATTCGGATGCTTTGCCAGGTACTCGCGCATAACGCGCTCGCCCTGTTTCATCAGCTc < 2:348935/151‑1 (MQ=255) cgcgGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTc > 1:432097/1‑151 (MQ=255) gcgGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCa < 1:234656/151‑1 (MQ=255) cgGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCAt > 2:323362/1‑151 (MQ=255) gCATCAGGCTCACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACACGCGCATAACGGGCACTCCCTGTTTCATCAGCTCa < 2:674552/148‑1 (MQ=255) tCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCtg > 2:125894/1‑151 (MQ=255) cacaAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGACCTGTTTCATCAGCTCATCGCTGGTGGTGATa > 1:512506/1‑149 (MQ=255) acaAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGc < 1:686689/151‑1 (MQ=255) acaAAATTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGc < 1:678737/151‑1 (MQ=255) aaaTTCGGATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTc < 2:606695/151‑1 (MQ=255) ggATTATGGGTGC‑CTTTTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAg < 2:373965/151‑1 (MQ=255) ggATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGc > 2:321779/1‑148 (MQ=255) ggATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGa < 2:686969/151‑1 (MQ=255) gATTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGAt < 1:24090/151‑1 (MQ=255) aTTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGAt > 1:271604/1‑150 (MQ=255) aTTATGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGAt > 1:149012/1‑150 (MQ=255) aTGGGTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGAtagtt < 2:278650/151‑1 (MQ=255) ggTGC‑CT‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGAtagttagt > 1:132782/1‑151 (MQ=255) | AATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCAGGAACCATTTCCGGTGCCAGGGTCACTTTGGTAATGACGTCGGCGTTTTCACACAGGAAATCGACCAGCGCGGCATCAGGCTTACGCACAAAATTCGGATTATGGGTGC‑C‑‑TTTTTTTACCAGATTCAGCCACGGACCTTCCAGATGCAGACCTAACGCCTGATTCGGATGTTTTGCCAGGTACTCGCGCATAACGCGCACGCCCTGTTTCATCAGCTCATCGCTGGTGGTGATAAGCGTCGGCAGATAGTTAGT > NC_000913/702185‑702475 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |