Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,486	0	C	A	44.4%	62.6 / 22.9	45	intergenic (+291/‑165)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base C (22/3);  new base A (0/20);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 6.32e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29337‑29633                                                                                                                                                      |                                                                                                                                                    ttttGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCcac                                                                                                                                                     >  1:502341/1‑150 (MQ=255)   ttgttgTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACaa                                                                                                                                                   >  2:672205/1‑150 (MQ=255)       tgttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaata                                                                                                                                               >  1:402829/1‑150 (MQ=255)        gttTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAaataa                                                                                                                                              <  2:454834/150‑1 (MQ=255)              aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                       <  2:310772/151‑1 (MQ=255)                 tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                    >  1:510658/1‑151 (MQ=255)                               ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                      >  1:305254/1‑151 (MQ=255)                                tGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                     >  2:396857/1‑151 (MQ=255)                                 ggTCCTTTTTTTTCTGCTCTTTTTTATTTCATGCAATCTTCTTGCTGCGCAGGCGTTTTCAAGAACAGGTTAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCATGGGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTg                                                                                                                     <  1:676903/150‑1 (MQ=255)                                       cTTTTTTCTGCTCTTTTTTATTTCATCCAATCTTCTTCCTGCCCAAGCTTTTCCCAGAACAGTTTAGATAATTTTTTTTCCGTTAAAGGCCTGAAAAACATGCAGGAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                <  2:598694/149‑1 (MQ=255)                                       cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                               >  2:63416/1‑150 (MQ=255)                                        ttttttCTGCTCTTTTTTTTTTAATGAAATCTTCTTGCTGCCAAAGCTTTTTCAAGAACAGTTTAAATAATTTTTTTGTCGCTTAATGCCTATAAAAAATCAATGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTa                                                                                                              <  2:448282/150‑1 (MQ=255)                                                     tttttATTTCATGAATTCTTCTTGCTGCGAAAGCTTTTCCAAAAACAGTTTAGAGTATTTTTTTGTCGTTAAATCCCTAAAAAACATGCATGACCCAAAAAATAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                      <  2:82098/145‑1 (MQ=255)                                                      ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                >  2:129798/1‑150 (MQ=255)                                                           tttCATGCAATCTTCTTGCTGCGCAAGCTTTTCCCAGAAAAGTTTAGATGATCTTTTTGTCGCTTAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                           <  1:515698/150‑1 (MQ=255)                                                                     tcttttTGCTCCGCAGGCTTTTTCAAAAACAGTTTAGATGATTTTTTTGTCGTTAAATCCCTAAAAAACAGGCAGGGCCCAAAAAAAAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                  <  1:658254/149‑1 (MQ=255)                                                                       ttcttGCTGCGCAAGCTTTTTCAAAACAAGATTAAATTATTTTTTTGTCGTTTAATCCTTTAAAAACATGCATGACCCAAAAAATAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                 <  2:445535/148‑1 (MQ=255)                                                                          ttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                              >  2:621501/1‑148 (MQ=255)                                                                          ttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACAGGCATGAGACACAAAATAATATAAACAATCCCGCCATTAAGTTGACTTTTAGCTCCCATATCTCCAGATTGCCGCCGTTTGcc                                                                              >  2:556887/1‑148 (MQ=255)                                                                           tgctgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                           >  2:144556/1‑150 (MQ=255)                                                                            tcggcgcAAGTGTTGTCCAAAACAGGTTAGATAATTTTTTTTCCTCTAAATCCCTAAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                           <  2:240/146‑1 (MQ=255)                                                                                cgcAAGCTTTTTCAAAAACAGGTAAGATGATTTTTTTGCCGTTTAAGGCCTGAAAACCATCCAGGAGCCAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                            <  1:402382/144‑1 (MQ=255)                                                                                 gcAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                    >  2:398845/1‑151 (MQ=255)                                                                                             aaaaaCAGTTTAGATTATTTTTTTTTCTTTTAATGCCTAAAAAAAATGCATGACCCAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                           <  2:321418/145‑1 (MQ=255)                                                                                              aGAACAGGTAAGATGATTTTTTTGTCGTTTAATGCTTGTAAACCATGCAGGGCCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAg                                                          <  1:143578/148‑1 (MQ=255)                                                                                                aaCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                     >  2:654936/1‑151 (MQ=255)                                                                                                      ttAGATTATTTTTTTGTCTCTTAATGCCTGAAAAACATGCTTGGCCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                <  2:339867/150‑1 (MQ=255)                                                                                                             atCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                        >  1:152409/1‑151 (MQ=255)                                                                                                              tttttttGTCGCTTAATGCCTGAAAAACATGAATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                          <  1:481737/146‑1 (MQ=255)                                                                                                                tttttGTCGCTTAATGCCTGTAAAACAAGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAGGtcat                                     >  2:59773/1‑151 (MQ=255)                                                                                                                   ttGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                  >  1:613944/1‑151 (MQ=255)                                                                                                                      tCGTTTAATGCCTTAAAAACATGCATGACCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                 <  1:85098/149‑1 (MQ=255)                                                                                                                          ttAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                           >  1:682174/1‑151 (MQ=255)                                                                                                                          ttAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                           >  1:511856/1‑151 (MQ=255)                                                                                                                          ttAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                           >  1:111793/1‑151 (MQ=255)                                                                                                                            aaTGCCTAAAAAACATGCATGCCCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                         <  1:397074/151‑1 (MQ=255)                                                                                                                             aTGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGCTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                         >  1:3092/1‑150 (MQ=255)                                                                                                                                 cTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                    >  2:492277/1‑151 (MQ=255)                                                                                                                                    aaaaaCAGACATGACACAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                 <  1:634371/150‑1 (MQ=255)                                                                                                                                     aaaaaaTCCATGCCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                    <  2:404812/147‑1 (MQ=255)                                                                                                                                     aaaaCTTGCATGAGCCAAAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa                <  2:502090/151‑1 (MQ=255)                                                                                                                                     aaaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                 >  2:17818/1‑150 (MQ=255)                                                                                                                                               tgAGCCAAAAAATAATATAAAAACGCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt          <  2:601036/147‑1 (MQ=255)                                                                                                                                                gAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt      <  2:497539/150‑1 (MQ=255)                                                                                                                                                 acccAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga        <  2:563993/145‑1 (MQ=255)                                                                                                                                                   ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat  >  1:365292/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                    TTTTGTTGTTTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAAT  >  NC_000913/29337‑29633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑