Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	29,496	0	T	A	44.7%	55.4 / 21.0	47	intergenic (+301/‑155)	dapB/carA	dihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (20/6);  new base A (0/21);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 2.41e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29350‑29645                                                                                                                                                   |                                                                                                                                                      aTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaa                                                                                                                                                   <  2:310772/151‑1 (MQ=255)    tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                                >  1:510658/1‑151 (MQ=255)                  ttGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGtt                                                                                                                                  >  1:305254/1‑151 (MQ=255)                   tGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTg                                                                                                                                 >  2:396857/1‑151 (MQ=255)                    ggTCCTTTTTTTTCTGCTCTTTTTTATTTCATGCAATCTTCTTGCTGCGCAGGCGTTTTCAAGAACAGGTTAGATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCATGGGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTg                                                                                                                                 <  1:676903/150‑1 (MQ=255)                          cTTTTTTCTGCTCTTTTTTATTTCATCCAATCTTCTTCCTGCCCAAGCTTTTCCCAGAACAGTTTAGATAATTTTTTTTCCGTTAAAGGCCTGAAAAACATGCAGGAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACttt                                                                                                                            <  2:598694/149‑1 (MQ=255)                          cTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACtttt                                                                                                                           >  2:63416/1‑150 (MQ=255)                           ttttttCTGCTCTTTTTTTTTTAATGAAATCTTCTTGCTGCCAAAGCTTTTTCAAGAACAGTTTAAATAATTTTTTTGTCGCTTAATGCCTATAAAAAATCAATGAGCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTa                                                                                                                          <  2:448282/150‑1 (MQ=255)                                        tttttATTTCATGAATTCTTCTTGCTGCGAAAGCTTTTCCAAAAACAGTTTAGAGTATTTTTTTGTCGTTAAATCCCTAAAAAACATGCATGACCCAAAAAATAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCat                                                                                                                  <  2:82098/145‑1 (MQ=255)                                         ttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                            >  2:129798/1‑150 (MQ=255)                                              tttCATGCAATCTTCTTGCTGCGCAAGCTTTTCCCAGAAAAGTTTAGATGATCTTTTTGTCGCTTAATGCCTGAAAAACATGCATGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                       <  1:515698/150‑1 (MQ=255)                                                        tcttttTGCTCCGCAGGCTTTTTCAAAAACAGTTTAGATGATTTTTTTGTCGTTAAATCCCTAAAAAACAGGCAGGGCCCAAAAAAAAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                              <  1:658254/149‑1 (MQ=255)                                                          ttcttGCTGCGCAAGCTTTTTCAAAACAAGATTAAATTATTTTTTTGTCGTTTAATCCTTTAAAAACATGCATGACCCAAAAAATAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                             <  2:445535/148‑1 (MQ=255)                                                             ttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                          >  2:621501/1‑148 (MQ=255)                                                             ttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACAGGCATGAGACACAAAATAATATAAACAATCCCGCCATTAAGTTGACTTTTAGCTCCCATATCTCCAGATTGCCGCCGTTTGcc                                                                                          >  2:556887/1‑148 (MQ=255)                                                              tgctgcGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                       >  2:144556/1‑150 (MQ=255)                                                               tcggcgcAAGTGTTGTCCAAAACAGGTTAGATAATTTTTTTTCCTCTAAATCCCTAAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGa                                                                                       <  2:240/146‑1 (MQ=255)                                                                   cgcAAGCTTTTTCAAAAACAGGTAAGATGATTTTTTTGCCGTTTAAGGCCTGAAAACCATCCAGGAGCCAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                                        <  1:402382/144‑1 (MQ=255)                                                                    gcAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                                >  2:398845/1‑151 (MQ=255)                                                                                aaaaaCAGTTTAGATTATTTTTTTTTCTTTTAATGCCTAAAAAAAATGCATGACCCAAAAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCa                                                                       <  2:321418/145‑1 (MQ=255)                                                                                 aGAACAGGTAAGATGATTTTTTTGTCGTTTAATGCTTGTAAACCATGCAGGGCCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAg                                                                      <  1:143578/148‑1 (MQ=255)                                                                                   aaCAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTg                                                                 >  2:654936/1‑151 (MQ=255)                                                                                         ttAGATTATTTTTTTGTCTCTTAATGCCTGAAAAACATGCTTGGCCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTg                                                            <  2:339867/150‑1 (MQ=255)                                                                                                atCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                    >  1:152409/1‑151 (MQ=255)                                                                                                 tttttttGTCGCTTAATGCCTGAAAAACATGAATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAc                                                      <  1:481737/146‑1 (MQ=255)                                                                                                   tttttGTCGCTTAATGCCTGTAAAACAAGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAGGtcat                                                 >  2:59773/1‑151 (MQ=255)                                                                                                      ttGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                              >  1:613944/1‑151 (MQ=255)                                                                                                         tCGTTTAATGCCTTAAAAACATGCATGACCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                             <  1:85098/149‑1 (MQ=255)                                                                                                             ttAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                       >  1:682174/1‑151 (MQ=255)                                                                                                             ttAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                       >  1:511856/1‑151 (MQ=255)                                                                                                             ttAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                       >  1:111793/1‑151 (MQ=255)                                                                                                               aaTGCCTAAAAAACATGCATGCCCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                     <  1:397074/151‑1 (MQ=255)                                                                                                                aTGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGCTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                     >  1:3092/1‑150 (MQ=255)                                                                                                                    cTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                                >  2:492277/1‑151 (MQ=255)                                                                                                                       aaaaaCAGACATGACACAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                             <  1:634371/150‑1 (MQ=255)                                                                                                                        aaaaaaTCCATGCCCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                                <  2:404812/147‑1 (MQ=255)                                                                                                                        aaaaCTTGCATGAGCCAAAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa                            <  2:502090/151‑1 (MQ=255)                                                                                                                        aaaaCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaa                             >  2:17818/1‑150 (MQ=255)                                                                                                                                  tgAGCCAAAAAATAATATAAAAACGCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagt                      <  2:601036/147‑1 (MQ=255)                                                                                                                                   gAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagt                  <  2:497539/150‑1 (MQ=255)                                                                                                                                    agacaaaaaaTGATAAAAAAAAGCCCATCATTAAGTTGACTTTTAGCGCCCATATCTGCAGAATGACGCCGTTTGCCAGAAATTCGTCTGTAAGCAGAATTGCATTGATGTACGTCATCATTGTGAATTAATATTCAAATAAAttgagtga                <  2:9121/145‑1 (MQ=255)                                                                                                                                    acccAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtga                    <  2:563993/145‑1 (MQ=255)                                                                                                                                      ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat              >  1:365292/1‑151 (MQ=255)                                                                                                                                        aaaaaaTAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAata             <  2:204910/148‑1 (MQ=255)                                                                                                                                          aaaaTAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt           <  1:125213/150‑1 (MQ=255)                                                                                                                                               aataAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg      <  1:80296/150‑1 (MQ=255)                                                                                                                                               aataAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTg      <  1:475674/150‑1 (MQ=255)                                                                                                                                                  ataAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAgg  >  1:293015/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                      ATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAGG  >  NC_000913/29350‑29645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑