Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	718,669	0	C	T	44.4%	38.8 / 17.4	36	E598K (GAA→AAA)	speF	ornithine decarboxylase isozyme, inducible
Reads supporting (aligned to +/- strand):  ref base C (5/15);  new base T (16/0);  total (21/15)
Fisher's exact test for biased strand distribution p-value = 3.22e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCC  >  NC_000913/718528‑718814                                                                                                                                              |                                                                                                                                                  gACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTTcaca                                                                                                                                           >  1:683007/1‑150 (MQ=255)   cGAACCAGTTCCACTGCACCGCGTAAATAGGCGTAGTTTGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATTCATTTCCTGACACAACt                                                                                                                                        <  2:399811/151‑1 (MQ=255)     aaCCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTACAAAAAATGTCTTTTCTGACACaa                                                                                                                                          >  2:559139/1‑147 (MQ=255)         aGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCa                                                                                                                                  <  1:533576/151‑1 (MQ=255)            tCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAgg                                                                                                                                <  1:274595/150‑1 (MQ=255)                cTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCGTACAAATCATGCATTTTCTGACACAACTGAAGCAggg                                                                                                                               >  2:118039/1‑147 (MQ=255)                          tAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAAACATGCATTTTCTTACACAACTGAAGCAGGGGATAAACGGCgga                                                                                                                  >  1:671790/1‑148 (MQ=255)                           aaaTAGGCGTAGTTGGCTTCTTGCGTATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTAGAATCGGCg                                                                                                                    <  2:151798/147‑1 (MQ=255)                                 gCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCtt                                                                                                          <  1:675804/151‑1 (MQ=255)                                     aGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTGACCCAACAGACGCAGGGGGTAACCGGCGCAgcgcccct                                                                                                          >  1:360055/1‑143 (MQ=255)                                      gTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGc                                                                                                     <  1:176236/151‑1 (MQ=255)                                           cttcttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAATCATGCATTTCCTGGCCCAAACGACGCAGGGGAGAACCCGCGTAGCGCTCTTCCTTCTGgtt                                                                                                >  1:340164/1‑151 (MQ=255)                                           cttcttGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACAACTGACGCAGGGGATAACCCGCGTAGCGCTCTTCATGCTGttt                                                                                                >  1:505783/1‑151 (MQ=255)                                                  cGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATTCATTTTCTGACACAACTGACGCAGGGGATAACCCGCGGAGCGCCCTTCATTCTGttttt                                                                                              >  1:403764/1‑144 (MQ=255)                                                     aTTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGAAACACATCATGCATTTTTTGCTCCAAATGACGCACGGGGTAAACCGGGGAGGGCGCTTCTTGTTGGTTGttg                                                                                            >  2:138030/1‑143 (MQ=255)                                                                        aaGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGCTACAAAACATGCCTTTTCTTTTACAAAACACGCCGGGGATAAACCGCGTAGCGCTCTTTATGCTGTTTTTTGATGGGAGGGAGGACCTCTTCTaa                                                                     >  2:476757/1‑149 (MQ=255)                                                                          gTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGGATACAAATCATTCATTTCCTGACACAAATGACGCAGGGGATAAACGGCGTAGCGCCCTTCATGCTGTTTGTAGATGGAAGGTAGGACTTCTGCTAATgg                                                                  >  1:212788/1‑150 (MQ=255)                                                                            gCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGgcgc                                                               <  2:488384/151‑1 (MQ=255)                                                                            gCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATAAAAAAAATGCATTTTCTGACACAAATGACGCAGGGGATAAACGGCGTAGCGCTCTTTATGCTGTTTTTTGATGGAAGGGAGGACTTTTTCTTATGgcg                                                                >  1:160958/1‑150 (MQ=255)                                                                              tCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGTATACAAAACATTCATTTTCTTACAAAACATACGCCGGGGGTAAACGGCGGCGCGGCCTTCATGCTGTTTTTTGATGGAAGGGAGCAATTCTTCTCATTgcg                                                                >  2:425678/1‑148 (MQ=255)                                                                              tCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATTCATTTTCTTACACAACTTACGCCGGGGGTAAACGGAGGAGCGCTCTTCTTGCTTTTTTTTGATGGAAGGGAGGACTTCTTCTTATGGcgcg                                                              >  2:169474/1‑150 (MQ=255)                                                                               ccTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGcgcg                                                              <  2:40797/149‑1 (MQ=255)                                                                                 ttACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACAACTGACGCCGGGGATAAACCGCGGAGCGCCCTTCATGCTGTTTTTAGATGGAAGGTAGCACTTCTTCTAATGGGGcggcgg                                                          >  1:574820/1‑151 (MQ=255)                                                                                   aCGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATAAAAAAACTTCATTTTCTTTAACAACTGCCGCAGGGGATAAACTGCGGAGGGCGCTTTCTGCTGTTTTTTGATGGAAGGGGGCACTTCTTCTAATAGCGCCTCgggc                                                        >  2:580179/1‑149 (MQ=255)                                                                                      gAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCCTACAAATCATGCATTTTCTTACACAAATGACGCAGGGGATAAACCGCGGAGGGCCCTTCCTGCTGTTTTTAGATGGGAGGGAGCACTTCTGCTAATTGCGCGTCGGAc                                                        >  1:330060/1‑148 (MQ=255)                                                                                         cATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAAACATGCATTTTCTTACACAAATGACGCAGGGGATAAACCGCGGAGCGCTCTTCATGCTGTTTGTAGATGGAAGGGAGCACTTCTGCTAATGGCGCGTCGGGCTcca                                                    >  1:373545/1‑147 (MQ=255)                                                                                                ttttGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGtttt                                            <  2:351960/150‑1 (MQ=255)                                                                                                ttttGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGttt                                             <  1:344812/149‑1 (MQ=255)                                                                                                 tttGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGttttt                                           <  2:447040/150‑1 (MQ=255)                                                                                                    gCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGaa                                       <  1:718169/151‑1 (MQ=255)                                                                                                    gCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGaa                                       <  2:501879/151‑1 (MQ=255)                                                                                                                             aTACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGc              >  1:116732/1‑151 (MQ=255)                                                                                                                                cAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTg            >  1:25014/1‑150 (MQ=255)                                                                                                                                     cATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGttt      <  2:247979/151‑1 (MQ=255)                                                                                                                                      aTGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGttt      >  2:41870/1‑150 (MQ=255)                                                                                                                                         cATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGcc  <  1:50352/151‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  GACGAACCAGTTCCACTTCACCGCGTAAATAGGCGTAGTTGGCTTCTTGCGGATTCATGCTGACGCGTGGGAAGTGCTCCTTACGGAACATCTCTTTTTGCAGTTGTTTCACGTTGTGGCGGGCATACAAATCATGCATTTCCTGACACAACTGACGCAGGGTATAACCGGCGTAGCGCTCTTCATGCTGTTTGTAGATGGAAGGTAGCACTTCTGCTAATGGCGCGTCGGACTCAAGCAGTTTTTCGAAGCGTACCAGCAGGGCAACAAGTTGCTGAAGTTTGGCC  >  NC_000913/718528‑718814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑