Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	941,713	0	T	G	46.2%	46.9 / 17.1	39	V252G (GTG→GGG)	dmsA	dimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (2/19);  new base G (18/0);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 3.35e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941569‑941862                                                                                                                                                  |                                                                                                                                                      actCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACt                                                                                                                                                   <  2:168466/149‑1 (MQ=255)       ccGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGCAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCtt                                                                                                                                                  >  1:96864/1‑144 (MQ=255)               aTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAgg                                                                                                                                   <  1:149857/151‑1 (MQ=255)               aTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAgg                                                                                                                                   <  2:377368/151‑1 (MQ=255)                  gCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCa                                                                                                                                 <  2:188794/150‑1 (MQ=255)                         gTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGaa                                                                                                                         <  2:68255/151‑1 (MQ=255)                           ttGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGaaa                                                                                                                        <  1:650564/150‑1 (MQ=255)                            tGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGACTTTCTATCTTTAACAAGCACCCCACaaa                                                                                                                        >  2:592449/1‑149 (MQ=255)                             gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTATCTTTAACAAGCCCGCCCGAAATCt                                                                                                                     >  2:271177/1‑151 (MQ=255)                                       tACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGG‑GGGGGGGGTTTCTCTCTTTTAAAAGGACGCCAGAAAACTAAtaacccc                                                                                                            >  1:666790/1‑144 (MQ=255)                                           gcggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGat                                                                                                       <  1:560582/151‑1 (MQ=255)                                            cggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGC‑GGGGGGAATTACTATCTTGAAAAAGGACGCCAGAAATCTAACACCCGc                                                                                                            >  1:556701/1‑145 (MQ=255)                                             ggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatca                                                                                                     <  1:586017/151‑1 (MQ=255)                                                tGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGACTTACAATCTTTAACAAGCCCCGCAGAAAACTAATTGCCGCATGatcat                                                                                                    >  2:341062/1‑149 (MQ=255)                                                            aaCAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGACTTACTATCTTTAACAAGCACGCCCGAAATCTAATTCCCGCATGATTCTCCTCCATCCgcgc                                                                                       >  1:136688/1‑150 (MQ=255)                                                                      cGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCTGCATGATCATCATCGATCCGCGCTATACCGaca                                                                             <  2:159802/150‑1 (MQ=255)                                                                      cGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATAcc                                                                                 >  1:126359/1‑146 (MQ=255)                                                                       ggATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACAcc                                                                           <  2:48896/151‑1 (MQ=255)                                                                         atatCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTATCTTTAAAAGGAACCCCCGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCCATACCGACACCgg                                                                         >  1:674345/1‑151 (MQ=255)                                                                           atCGAAAACAGTACGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGC‑GGGGGGACTTACTACTTTGAACAGGCACGCCAGAAAAATAAAGCCCCCATGATTATCCACTATCCCCGCTATACCAGCAcc                                                                           >  1:218329/1‑147 (MQ=255)                                                                              gAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGGCTTACTATCTTTAACAGGCACGCCCGAAATCTAATGCCCCCATGAACATCATCGATCCGCGCTATACCCACAcccggggc                                                                     >  1:240650/1‑144 (MQ=255)                                                                                   cAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcgc                                                               <  2:431215/151‑1 (MQ=255)                                                                                   cAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcgc                                                               <  1:311781/151‑1 (MQ=255)                                                                                     gTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGa                                                             <  1:157271/151‑1 (MQ=255)                                                                                         gCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGAt                                                         <  1:236928/151‑1 (MQ=255)                                                                                              tAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGG‑GGGGGGACTTACTATCTTTAAACGGCAACCCAGAAAAATAATGGCCCCCTGATCATCATCGAACCGCGCTATAACCACACccgggccggg                                                                  >  2:689610/1‑137 (MQ=255)                                                                                               aGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTgg                                                   <  1:711444/151‑1 (MQ=255)                                                                                                gTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGACTTACTATCTTGAAAAGGCACGCCAGAAAACTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCGACACCGGGGGCGGGGGCGAAGATGAGTgg                                                   >  1:220741/1‑150 (MQ=255)                                                                                                      tttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGACTT‑CTATCTTTAACAGGGCCCCCAGAAAACTAATGCCCCCCAGATCATTCCCCATACCCGCTATAACCACACCCGGGCCGGGCGCGGAGATGAGTgggctcc                                              >  1:372133/1‑144 (MQ=255)                                                                                                      tttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAAAGCCCGCATGATCATCATCCATCCCCGCCATACCGACACCGGGGCCGGGCGCGAAGATGAGGGGGTccc                                              >  2:498909/1‑149 (MQ=255)                                                                                                         ggTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTAtt                                          <  2:411024/150‑1 (MQ=255)                                                                                                           taataaCCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGACTTACTATCTTGAACAAGCACGCCAGAAAACTAAAGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATGAGTGGGTCCCCAtt                                          >  2:640308/1‑148 (MQ=255)                                                                                                                    tGGCGAAACGCGAATGAGTGGCGGT‑GGGGGGACTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCTATACCCACACCGGTGCCGGGGGCGAAGATGAGTGGGTCCCTATTCGTCCgggga                                >  1:140745/1‑147 (MQ=255)                                                                                                                       cGAAACGCGAATGAGTGGCGGT‑GGGGGGACTTACTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCCACACCGGTGCCGGGGGCGGAGATGAGTGGGTCCCTATTCGTCCggg                                  >  1:483621/1‑144 (MQ=255)                                                                                                                         aaaCGCGAATGAGTGGCGGT‑GGGGTGAACTACTATCTTTAAAAAGCACGCCCGAAATCTAATGCCCGCAAGAACATCATCGAACCGCGCCCTACCGACACCCGGGCCGGGCGCGGAGAGGAGTGGATCCCTATTCGTCCGGGGACAGaag                          >  2:655084/1‑148 (MQ=255)                                                                                                                          aaCGCGAATGAGTGGCGGT‑GGGGGGACTTACTATCTTTAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGGGGATCCCTATTCGTCCGGGGACAGATGcc                        >  1:396348/1‑151 (MQ=255)                                                                                                                                   gAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGtt               <  2:489177/151‑1 (MQ=255)                                                                                                                                                 gTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt  <  2:672339/151‑1 (MQ=255)                                                                                                                                                 gTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGt  <  1:630934/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                      ACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGT‑GGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACGGTCTGGCGT  >  NC_000913/941569‑941862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑