Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,079,468	0	A	G	43.9%	53.2 / 12.9	43	D55G (GAT→GGT)	putP	proline:sodium symporter
Reads supporting (aligned to +/- strand):  ref base A (2/21);  new base G (18/0);  total (22/21)
Fisher's exact test for biased strand distribution p-value = 9.40e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAA  >  NC_000913/1079334‑1079618                                                                                                                                       |                                                                                                                                                         aTTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGGATGAGCggccgg                                                                                                                                             >  1:83571/1‑148 (MQ=255)    ttGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGtt                                                                                                                                        <  2:303735/150‑1 (MQ=255)     tgtgTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGTCGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTa                                                                                                                                       <  2:152512/150‑1 (MQ=255)      gtgtCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGGATGAGGGGGGGGGTGTTTAt                                                                                                                                     >  1:243829/1‑151 (MQ=255)        gtCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATgg                                                                                                                                   <  1:529951/151‑1 (MQ=255)               tCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGCGGGGGGCTGTTTATgggg                                                                                                                                 >  1:555291/1‑146 (MQ=255)                cTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGGATGAGGGGGTGGGTGTTTATgggg                                                                                                                                 >  1:34316/1‑145 (MQ=255)                 tttGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGCGCGGGGGTTATGGGCGGGGGGGTGTTAATGGGGGTGGCgg                                                                                                                          >  2:244320/1‑151 (MQ=255)                  ttGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGc                                                                                                                             <  2:103844/147‑1 (MQ=255)                  ttGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCATCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCgg                                                                                                                          <  2:622045/150‑1 (MQ=255)                  ttGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGATATGAGGGGGTGGGTGGTTATGGGGGTGGCggg                                                                                                                         >  2:713262/1‑151 (MQ=255)                   tGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCtgg                                                                                                                         <  2:622914/150‑1 (MQ=255)                   tGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGCGGGTGGGTGTTTATGGGGGTGGCggg                                                                                                                         >  1:594480/1‑150 (MQ=255)                   tGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGCGGGTGGGTGTTTATGGGGGTGCCggg                                                                                                                         >  1:350927/1‑150 (MQ=255)                         gATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTGTGAGGGGCGGGGTGTTTATGGGGGTGCCggg                                                                                                                         >  1:483756/1‑144 (MQ=255)                            attgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑tttt                                                                                                              <  1:32117/150‑1 (MQ=255)                             ttgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGGTCGGTATTGGGGGGGTGGGCGTTAATGGGGGCGGCGGGGG‑CTG‑tgtt                                                                                                              >  2:682553/1‑149 (MQ=255)                             ttgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑ttttt                                                                                                             <  2:177743/150‑1 (MQ=255)                              tgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGGTCGGGTGTAAGGGGGGGGCTGTTTATTGGGGTGGCCGGCG‑CGG‑tgttt                                                                                                             >  1:321757/1‑149 (MQ=255)                              tgattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGGGGGCGGGGGTTTATGGGGGTGGCGGGGG‑GTG‑ttttttt                                                                                                           >  1:663973/1‑149 (MQ=255)                               gattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTtttctt                                                                                                          <  1:289719/151‑1 (MQ=255)                               gattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTtttct                                                                                                           <  1:686532/150‑1 (MQ=255)                               gattgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTtttct                                                                                                           <  1:373216/150‑1 (MQ=255)                                  tgGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGTCGGGTATGAGCGGGTGGGTGTTTATGGGGGTGGCGGGCG‑CTG‑Gtttttttt                                                                                                         >  1:571875/1‑149 (MQ=255)                                         aTCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGGGGGGGGGTGTTTATTGGGGTGGCGGGCG‑CTG‑GTTTTTTTTTCgggggtt                                                                                                >  1:146226/1‑147 (MQ=255)                                             ccTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGCGGCGGGTATGAGGGGGTGGGTGGTTATGGGGGTGGCGGGGG‑GGG‑TTTTTTTTTTCgggggt                                                                                                 >  1:679996/1‑143 (MQ=255)                                                  cGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGCGCGGGTTTTGGGGGCTGGGTGTTTATAGGGGTGCCGGGGGCCGG‑TTTTTTTTTCCGGGTTTTCCGaa                                                                                           >  2:471324/1‑148 (MQ=255)                                                      cAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGa                                                                                    <  2:189647/150‑1 (MQ=255)                                                      cAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATTGGCGGCTGGCTGTTTTTGGGGGTGTCGCGGG‑CGG‑TTTTTTTTTTCGGGATTTTCgtgaagt                                                                                       >  2:707242/1‑141 (MQ=255)                                                       aaCGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATc                                                                                  <  1:490802/151‑1 (MQ=255)                                                           aaaaaCTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCa                                                                              <  2:277984/151‑1 (MQ=255)                                                                 tttGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGGGGCGGGTATTTGGGGGTGGGGGTTTATGGGGGTGGCCGGCG‑CCG‑TTTTTTTTTTCGGGGGTTTCGGCAGCTGGGTCGCCAtttg                                                                          >  2:37180/1‑147 (MQ=255)                                                                     acgacTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGTCGGGTATGAGCGGCTGGGTGTTTATGGGGGTGCCGGGGG‑GTGTTTTTTTTTTCCGGGTTTTCCCAAA‑ATGGAGCGCCCTTGGCCTGAc                                                                    >  1:627134/1‑151 (MQ=255)                                                                           atatTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTa                                                                <  2:707203/149‑1 (MQ=255)                                                                           atatTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAgg                                                              <  1:241378/151‑1 (MQ=255)                                                                                  gggCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTgg                                                       <  2:336354/151‑1 (MQ=255)                                                                                     cGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGAtt                                                    <  1:699428/151‑1 (MQ=255)                                                                                      ggTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGGGGGGCGGGTATGAGGGGGCGGGTGTTAATGGGGGTGGCGGGGG‑CGG‑GTTTTTTTTTCGGGGTTTTCCAAAACTGGAACGGCAATTGGCTGGCCTTAGGCGCGCgg                                                       >  1:284500/1‑147 (MQ=255)                                                                                             gTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGaa                                            <  1:225513/151‑1 (MQ=255)                                                                                                        ttCGTGACGGCATTATCGGCGGGTGCGCCGGGGATGAGAGGGTGGCCGTTTATTGGGGTGGCGGGGG‑CCG‑GTTTTTTTTCCGGGGGTTCCGAAAACTGGATCGCCCTTGGGCCGACATTAGGCGGGCGGGTTTACTGGAAGCtggtgg                                    >  1:386253/1‑148 (MQ=255)                                                                                                                      aTCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCgtgt                    <  2:376415/150‑1 (MQ=255)                                                                                                                       tCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGtgtg                   >  1:457514/1‑150 (MQ=255)                                                                                                                                     ggATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATacaa     <  2:172007/150‑1 (MQ=255)                                                                                                                                       atatGGGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGAATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATacaa     <  2:78388/148‑1 (MQ=255)                                                                                                                                       atatGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATAcaacaa  <  1:365968/151‑1 (MQ=255)                                                                                                                                       |                                                                                                                                                         ATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCATTATCGGCGGGTGCGTCGGATATGAGCGGCTGGCTGTTAATGGGGTTGCCGGGCG‑CTG‑TTTTTCTTTCCGGGATTTCCGAAAGCTGGATCGCCATTGGCCTGACATTAGGCGCGTGGATTAACTGGAAGCTGGTGGCCGGGCGGTTGCGTGTGCATACCGAATACAACAA  >  NC_000913/1079334‑1079618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑