Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	64,352	G→C	R477G (CGC→GGC)	polB ←	DNA polymerase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	64,352	0	G	C	100.0%	132.8 / NA	36	R477G (CGC→GGC)	polB	DNA polymerase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (19/17);  total (19/17)

TCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTG  >  NC_000913/64205‑64494                                                                                                                                                    |                                                                                                                                               tCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGCGCCGAGCACGCCACAAAAGGCATTCATGATGATTTTCGGCGCCTGCAACAGCGGTTTGTTACCCCGGCGTTTGGCTTCATCGCcccc                                                                                                                                             >  2:578019/1‑151 (MQ=255)    gatgaCCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTg                                                                                                                                          <  1:239460/151‑1 (MQ=255)           aCGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATg                                                                                                                                   >  1:439645/1‑151 (MQ=255)                  gTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTca                                                                                                                            <  2:236190/151‑1 (MQ=255)                        gacgaTGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAAtct                                                                                                                      <  1:585639/151‑1 (MQ=255)                        gacgaTGCAAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAAt                                                                                                                        <  1:240522/149‑1 (MQ=255)                         acgacgCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACCCCCTAAAAGGCATTCATGATGTTTTTCAGCGCCTGCGCCAGCCGATTGTTACCCTGGAGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAAt                                                                                                                        >  2:521017/1‑148 (MQ=255)                         acgaTGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAAtctc                                                                                                                     >  1:671143/1‑151 (MQ=255)                                cAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCggcagg                                                                                                              >  1:298016/1‑151 (MQ=255)                                    cgcgGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAAt                                                                                                          >  2:560450/1‑151 (MQ=255)                                      cgGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGt                                                                                                        <  2:578579/151‑1 (MQ=255)                                        gATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGttt                                                                                                      >  2:279268/1‑151 (MQ=255)                                         aTCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGtttt                                                                                                     <  2:282056/151‑1 (MQ=255)                                          tCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGttttt                                                                                                    >  1:553996/1‑151 (MQ=255)                                                  gCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGTTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCga                                                                                             <  1:403152/150‑1 (MQ=255)                                                   cGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCga                                                                                             <  2:228570/149‑1 (MQ=255)                                                       aGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCa                                                                                       <  1:269819/151‑1 (MQ=255)                                                                   cGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCgaga                                                                             >  1:567392/1‑149 (MQ=255)                                                                        aCGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCtt                                                                      >  1:712398/1‑151 (MQ=255)                                                                        aCGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCtt                                                                      <  1:357170/151‑1 (MQ=255)                                                                                       ttCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTg                                                       >  1:642010/1‑151 (MQ=255)                                                                                        tCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTg                                                       <  2:506478/150‑1 (MQ=255)                                                                                             atgatTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAg                                                 <  2:69078/151‑1 (MQ=255)                                                                                                    tCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTgcgc                                          >  2:475857/1‑151 (MQ=255)                                                                                                    tCAACGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTgcgc                                          >  2:59060/1‑151 (MQ=255)                                                                                                        cgcCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATg                                      <  1:386125/151‑1 (MQ=255)                                                                                                                aCAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTccac                              >  1:266428/1‑151 (MQ=255)                                                                                                                 cAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTccacc                             >  2:401619/1‑151 (MQ=255)                                                                                                                  aGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTccacc                             >  1:65845/1‑150 (MQ=255)                                                                                                                      gTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGccc                        >  1:337005/1‑151 (MQ=255)                                                                                                                      gTTTGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGccc                        >  1:652909/1‑151 (MQ=255)                                                                                                                        ttGTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCg                       >  2:528921/1‑150 (MQ=255)                                                                                                                          gTTACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACg                    <  2:549703/151‑1 (MQ=255)                                                                                                                            tACCCTGGCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCGATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACgg                   <  2:501374/150‑1 (MQ=255)                                                                                                                                   gCGTTTGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGatcaatc           <  1:660915/151‑1 (MQ=255)                                                                                                                                        tGGCTTCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGaa       <  1:325060/150‑1 (MQ=255)                                                                                                                                            ttCATCGCCCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTg  >  2:535361/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                               TCTGATGACCACGCATGGTGATCGACGATGCCAGCCGCGGATCGAAGAAGCGGCAGGCGGTGGTGCCGAGCACGCCATAAAAGGCATTCATGATGATTTTCAGCGCCTGCGACAGCGGTTTGTTACCCTGGCGTTTGGCTTCATCGCGCCCGTGCCAGATGTTAGTCACAATCTCCGGCAGGCAATGTTTTTCTCGCGAGAACCAGGCATCGAGAAAACCTTCGGTACTGTGCTCTGGATCAGGCTGCGCCATGCCTTCCACCAGCCCGACGGGATCAATCAGAAAGGTG  >  NC_000913/64205‑64494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑