Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,725,232	C→A	G172V (GGA→GTA)	kgtP ←	alpha‑ketoglutarate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,725,232	0	C	A	100.0%	139.6 / NA	38	G172V (GGA→GTA)	kgtP	alpha‑ketoglutarate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/19);  total (19/19)

AGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGG  >  NC_000913/2725084‑2725377                                                                                                                                                     |                                                                                                                                                  aGTTTAATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTacg                                                                                                                                                 >  2:151680/1‑151 (MQ=255)      cATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGAt                                                                                                                                            <  2:539145/151‑1 (MQ=255)          tAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAAc                                                                                                                                        >  1:38676/1‑151 (MQ=255)          tAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAAc                                                                                                                                        >  1:655944/1‑151 (MQ=255)            aCTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGt                                                                                                                                      <  1:414417/151‑1 (MQ=255)                       aaCCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGaa                                                                                                                           <  1:568936/151‑1 (MQ=255)                           acaACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAatga                                                                                                                       <  2:640542/151‑1 (MQ=255)                               cGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCg                                                                                                                   >  1:276130/1‑151 (MQ=255)                                     aacaGCTAACACAGCTCCTAACGCGAAGGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACATCAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACGTACTGAAATGATGCGTaaaa                                                                                                              <  2:409402/150‑1 (MQ=255)                                         gCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCtt                                                                                                         <  2:566073/151‑1 (MQ=255)                                           tAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTg                                                                                                       >  1:55184/1‑151 (MQ=255)                                                     ccTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCaa                                                                                             <  2:548282/151‑1 (MQ=255)                                                        aaCGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACgg                                                                                          <  1:74247/151‑1 (MQ=255)                                                             gAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAAc                                                                                      >  1:330295/1‑150 (MQ=255)                                                                  gAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACt                                                                                <  2:505056/151‑1 (MQ=255)                                                                    aTACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTc                                                                               >  2:150472/1‑150 (MQ=255)                                                                          cATCCCCACTCGCGGAGTGCAGCGGCTTCCATGGTGTGTTGTAAAACCACTACAACCACTAGGTCTACCAGTTGTACGCCGGTCAAGGTCACATACTGAAATGATGCGTAAAAACCTTTACGACCTTCAACGTCAACTTCGCTCAtata                                                                          <  2:174503/149‑1 (MQ=255)                                                                            tCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTg                                                                      >  2:461686/1‑151 (MQ=255)                                                                                      ctGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAGCCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGTCGCTgg                                                               <  2:183565/148‑1 (MQ=255)                                                                                          gTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCat                                                         >  1:689626/1‑150 (MQ=255)                                                                                              aGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATtc                                                     >  2:51246/1‑150 (MQ=255)                                                                                                  tCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGc                                                >  1:592234/1‑151 (MQ=255)                                                                                                   cTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGcc                                               >  1:158415/1‑151 (MQ=255)                                                                                                      ccATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCaa                                             <  2:236897/150‑1 (MQ=255)                                                                                                       cATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACa                                           <  2:60680/151‑1 (MQ=255)                                                                                                        aTGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAAc                                            >  2:682333/1‑149 (MQ=255)                                                                                                           gtgtgtTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGAt                                        >  1:528462/1‑150 (MQ=255)                                                                                                                   tAAAACCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTg                                >  1:506868/1‑150 (MQ=255)                                                                                                                    aaaaCCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGa                               >  2:167544/1‑150 (MQ=255)                                                                                                                      aaCCACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGaaa                             >  2:8422/1‑150 (MQ=255)                                                                                                                        ccACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATaa                          <  1:448970/151‑1 (MQ=255)                                                                                                                         cACGACAACCAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATaa                          >  2:717418/1‑150 (MQ=255)                                                                                                                                 ccAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAAcgagcgag                 <  1:302870/151‑1 (MQ=255)                                                                                                                                  cAGTAGGGCTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCgaga                <  2:451118/151‑1 (MQ=255)                                                                                                                                          cTAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaata         >  1:560934/1‑150 (MQ=255)                                                                                                                                           tAGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaataat       >  2:345437/1‑151 (MQ=255)                                                                                                                                            aGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCaataa        <  2:192101/149‑1 (MQ=255)                                                                                                                                            aGCAGTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATg      <  1:187968/151‑1 (MQ=255)                                                                                                                                                gTTGTACGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCgg  <  2:410446/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  AGTTTCATCTAACTGACGACGTAACCACAACGCCACAACAGCTAACACAGCTCCTAACGCGAAAGGAATACGCCATCCCCACTCTCTGAGTGCAGCGTCTTCCATGGTGTGTTGTAAAACCACGACAACCAGTAGGGCTAGCAGTTGTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGG  >  NC_000913/2725084‑2725377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑