| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,804,926 | (A)6→5 | coding (112/1203 nt) | proV → | glycine betaine/proline ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,804,921 | 0 | A | . | 98.2% | 262.1 / ‑1.5 | 58 | coding (107/1203 nt) | proV | glycine betaine/proline ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base . (26/30); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAA > NC_000913/2804771‑2805066 | gggTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGa < 2:15791/151‑1 (MQ=255) tatatCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGaaaaact > 1:499641/1‑149 (MQ=255) tAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGc > 2:159130/1‑151 (MQ=255) tAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGc > 1:59729/1‑151 (MQ=255) aaaTAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGc > 2:699123/1‑151 (MQ=255) aaaGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTaa < 1:97693/151‑1 (MQ=255) ttCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGcc > 1:319412/1‑149 (MQ=255) tCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGt > 1:80023/1‑151 (MQ=255) tATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCt > 1:132289/1‑151 (MQ=255) tATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCt > 1:45640/1‑151 (MQ=255) aTTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCt < 1:667331/150‑1 (MQ=255) aTTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTg > 2:290419/1‑151 (MQ=255) ttGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTgg > 1:366127/1‑151 (MQ=255) cATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGcc > 1:595901/1‑150 (MQ=255) ggCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTg > 2:334074/1‑151 (MQ=255) gCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTga < 1:561754/151‑1 (MQ=255) aattaaattaGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTga > 1:422828/1‑149 (MQ=255) attaaattaGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTga > 1:625802/1‑148 (MQ=255) aGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCgag < 1:685087/150‑1 (MQ=255) aaaTTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGata < 1:681262/151‑1 (MQ=255) aaTTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGatat > 2:621201/1‑151 (MQ=255) tAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTg > 1:21245/1‑151 (MQ=255) aaaaaTCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTg < 2:675572/150‑1 (MQ=255) tataAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATggg > 1:361928/1‑151 (MQ=255) tataAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATggg > 2:126233/1‑151 (MQ=255) ataAAATATTTCGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAATAACTTTCAAAAGAACAAATTCT‑GCAAAAACTAAACTATCTCTTGTCGTAAAAGACGCCAGTCTTTCCATTCAAAAAGACAATATATTTGTcatcatttta < 1:369247/151‑5 (MQ=255) taAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGAt > 1:187945/1‑151 (MQ=255) taAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGAt > 2:307437/1‑151 (MQ=255) aaaaTATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGAt > 1:589105/1‑151 (MQ=255) aaaaTATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGAtt < 2:127220/151‑1 (MQ=255) aaTATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTAt < 1:89735/151‑1 (MQ=255) aaTATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTAt > 1:305721/1‑151 (MQ=255) atTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATcc < 1:22114/150‑1 (MQ=255) atTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCg > 1:405645/1‑151 (MQ=255) ggCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCt < 2:718885/150‑1 (MQ=255) aGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGt < 1:138652/151‑1 (MQ=255) gCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTa < 1:21580/151‑1 (MQ=255) cATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTaa < 2:255969/151‑1 (MQ=255) aTCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTaa > 1:195828/1‑150 (MQ=255) ccACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATc < 2:687476/151‑1 (MQ=255) acaGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCca < 2:507684/151‑1 (MQ=255) agcgagcgTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCcaca < 2:77104/151‑1 (MQ=255) cgagcgTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAAt < 2:289684/151‑1 (MQ=255) gcgTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGACTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGt < 2:588205/151‑1 (MQ=255) gTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTAc < 2:106054/151‑1 (MQ=255) atatCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTtct < 1:639214/150‑1 (MQ=255) atCGAACAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCaa < 1:728732/151‑1 (MQ=255) cAAGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCt < 1:415374/151‑1 (MQ=255) aaGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTg < 2:386222/151‑1 (MQ=255) aaGGACTTTCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTg < 2:617577/151‑1 (MQ=255) tttCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGa > 1:247682/1‑150 (MQ=255) tCAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAacc > 2:351557/1‑151 (MQ=255) cAAAAGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAaccc < 2:90817/151‑1 (MQ=255) aaaaGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGTGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAaccc > 1:455950/1‑150 (MQ=255) aaaaGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAaccca < 1:438346/151‑1 (MQ=255) aaaGAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAacccac > 1:622101/1‑151 (MQ=255) gAACAAATTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAACCCACCcg < 1:266627/151‑1 (MQ=255) aTTCTGG‑AAAAACTGTGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCAGGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAACCCTCCCGCGGGCa < 1:520509/151‑1 (MQ=255) aTTCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCa < 1:257038/151‑1 (MQ=255) tCTGG‑AAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCaa < 1:103970/150‑1 (MQ=255) | GGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATATATCGAACAAGGACTTTCAAAAGAACAAATTCTGGAAAAAACTGGGCTATCGCTTGGCGTAAAAGACGCCAGTCTGGCCATTGAAGAAGGCGAGATATTTGTCATCATGGGATTATCCGGCTCGGGTAAATCCACAATGGTACGCCTTCTCAATCGCCTGATTGAACCCACCCGCGGGCAA > NC_000913/2804771‑2805066 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |