Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,421,3970GA46.7% 49.3 / 30.4 45E25K (GAA→AAA) folXD‑erythro‑7,8‑dihydroneopterin triphosphate 2'‑epimerase and dihydroneopterin aldolase
Reads supporting (aligned to +/- strand):  ref base G (22/2);  new base A (0/21);  total (22/23)
Fisher's exact test for biased strand distribution p-value = 7.29e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTT  >  NC_000913/2421255‑2421542
                                                                                                                                              |                                                                                                                                                 
gTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTaac                                                                                                                                           >  2:65034/1‑151 (MQ=255)
             aCAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGat                                                                                                                               >  2:536197/1‑150 (MQ=255)
              cAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGatat                                                                                                                             >  2:404373/1‑151 (MQ=255)
              cAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACAGTCAGGatat                                                                                                                             >  1:218368/1‑151 (MQ=255)
               aGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATAtt                                                                                                                            >  1:162676/1‑151 (MQ=255)
               aGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATAtt                                                                                                                            <  1:86600/151‑1 (MQ=255)
                     aGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATc                                                                                                                      >  1:61248/1‑151 (MQ=255)
                              tgtATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACg                                                                                                             >  2:282146/1‑151 (MQ=255)
                                   tattTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATcc                                                                                                         >  2:116834/1‑150 (MQ=255)
                                    attTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCa                                                                                                        <  1:314046/150‑1 (MQ=255)
                                      ttCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACt                                                                                                      >  1:237602/1‑150 (MQ=255)
                                      ttCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACt                                                                                                      >  1:386293/1‑150 (MQ=255)
                                         aTCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAccc                                                                                                  >  2:430824/1‑151 (MQ=255)
                                          tCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAcccc                                                                                                 >  2:80892/1‑151 (MQ=255)
                                          tCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAcccc                                                                                                 >  1:68947/1‑151 (MQ=255)
                                          tCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTAcccc                                                                                                 >  1:212157/1‑151 (MQ=255)
                                               aGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCccgccg                                                                                             >  1:373367/1‑150 (MQ=255)
                                                 tAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGAt                                                                                           >  1:222645/1‑150 (MQ=255)
                                                          ggAGACCCTGAAAGGGCACACCCTGCCGTTATTATTCGAAAAAAGACCCTTCGTTTGCGTACGTTTATCGGAATTAAGAAAAAAAAAATAAACACCCGTAAGGATTTTGTTATAAGTGTGACGATCCACTCCCCCGCCGATAAAgcgc                                                                                    <  1:304540/148‑1 (MQ=255)
                                                              acccTGAAAGGCCACACCCGGCCGTTATTATTCTTAAAAAGACCCTTCTTTTGCGTACGTTTATGGGAATTAAGGAAAAAAAAATAAACACCCGTCAGGATATTGTTATAAATGTGCCGACCCACTCCCCCGCCGATAAAGCGCGCACTAg                                                                             <  2:291986/149‑1 (MQ=255)
                                                              aaCCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACt                                                                               >  1:434512/1‑149 (MQ=255)
                                                                ccgacAATGCCCCACCCTCCCGTTTTTATTCATAAAAAAACCTTTCTTTTGCGTCCTTTTATCAAAATAAAGAAAGAAAAAATAAACACCCGACAGGATTTTTTTATAAATGTGACATCCCACTCCCCCGCCGATAAAGCGCGCACTAGCg                                                                           <  2:421340/147‑1 (MQ=255)
                                                                  tGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGa                                                                          >  1:463656/1‑150 (MQ=255)
                                                                     aaTGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGa                                                                          >  2:177391/1‑147 (MQ=255)
                                                                         acacccccggCCGTTTTTTTTCATAAAAAGACCTTTGTTTTGTGTATTTTTATCGAATTAAAGAAAAAAAAAATAAACACCCGACGAGTTTTTTTTATAAATGGGCCGGCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATc                                                                   <  2:542805/141‑1 (MQ=255)
                                                                            caACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACg                                                               >  1:391365/1‑151 (MQ=255)
                                                                             acccggccttttttattCAAAAAAAAACCCTTCTTTTGCTTATTTTTATCGAAATAAAGAAAAAAAAAATAACCACCCGTCAGGATTTTGTTATAAATGAAACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGatat                                                                    <  1:413317/133‑1 (MQ=255)
                                                                               ctggCCGTTTTTTTTCTTAAAAAAACCTTTCTTTTGCGTACGTTTATAGAAATTAAGAAAAAAAAAATAAACACCGGTAAGGATATTTTTAAAAATGTGACGACCCTCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGAt                                                             <  1:164726/147‑1 (MQ=255)
                                                                               cctccctttattttTCGTAAAAAAACCCTCTTTTTGCTTACGTTTAGCGAAATAAAGAAAAAAAAAATAAACACCCGTAAGGATTTTTTAAAAAGTGGGACGACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGAt                                                             <  2:70488/142‑1 (MQ=255)
                                                                                 ggcctttattatTCGTAAAAAAACCCTTCTTTTGCGTTCGTTTATGGAAATAAAGAAAAAAAAAATAAACACCCGTAAGAATATTGTTATAAATGTAACAACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATgcg                                                          <  1:183133/145‑1 (MQ=255)
                                                                                    cGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTg                                                       >  2:183924/1‑151 (MQ=255)
                                                                                         ttattaaaaaaaaGACCTTTCTTTGGCTTATTTTTATCGAAATAAAGAAAAAAAAAAACAACAACATTCAGAATATTGTTATAAATGTGACGACCCACCCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAAt                                                    <  1:681851/139‑1 (MQ=255)
                                                                                         ttatgCGTATAAAAACCCTTGTTTTGCGTACGTTTATCGAAATAAAAGAAGAAAAAATTAACACCCGTCAGGATTTTGTTAAAAATGGGACGACCCACAACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAAtt                                                   <  1:500255/150‑1 (MQ=255)
                                                                                                 aaaaaaacccTTCTTTTGCGTCCGTTTATCGAAATAAAGAAAAAAAAAATAAACCCCCTCCAGAATATTTTTACCAATGTGACACCCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTa                                                  <  2:448185/135‑1 (MQ=255)
                                                                                                 aaaaaGACCCTTCTTTTGCGTTCGTTTATCGAAATAAAGGAAGAAAAAATAAACACCCGTCAGTATTTTGTTATCAATGTGACGATCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCg                                          <  1:58817/149‑1 (MQ=255)
                                                                                                   aaaaacctttCGTGTGCGTCGGTTTATGGAAATAAAGAAAAAAAAAATAAACACCCGTAAGGATATTTTTATAATTGTGACAACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTa                                        <  2:566679/143‑1 (MQ=255)
                                                                                                        cctttCTTTTGCGTCCGTTTATGGAAATAAAGGAAAAAAAAATAAACACCCGTCAGGATATTGTTATCAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGa                                    <  2:491561/147‑1 (MQ=255)
                                                                                                         cctccttttGCGTCTTTTTATCGAAATTAAGAAAAAAAAAATAAACACCCGTAAGGATATTTTTAACAATGTGACACCCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGa                                    <  1:404277/143‑1 (MQ=255)
                                                                                                           ttggTTTGCGTACTTTGAAGAGAATAAAGAAAAAAAAAATAAACAGCCGTGAGGATTTTGTTAAAAGTGAGACGAACCACTGCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGaaaa                                 <  1:640735/147‑1 (MQ=255)
                                                                                                                  gCGTATTTTTATCGAAATTAAGAAAAAAAAAATAAACACCCGAAAGGATTTTGTTATAAATGTGACGACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACatta                           <  1:646614/149‑1 (MQ=255)
                                                                                                                     tACGTTTATCGAAATAAAGGAAAAAAAAATAAACACCCGTCAGAATTTTTTTATCAATGTGACGATCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACatt                            <  2:359933/145‑1 (MQ=255)
                                                                                                                        ttttATCGAAAATAAGAAAAAAAAAATCAACACCCGGCAGGATTTTTTTATCAATGTGACCACCCCCTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTc                        <  1:381230/145‑1 (MQ=255)
                                                                                                                          ttatgaaaaTAAAGAAAAAAAAAATAAACACCCGTCAGGATATTTTTATAAATGTAACCACCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAg                      <  1:116808/139‑1 (MQ=255)
                                                                                                                             tCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGt                 >  2:339950/1‑148 (MQ=255)
                                                                                                                              cgaaaTAAAGAAAAAAAAAATAAACACCCGTCAGGATATTTTTATCAATGTGACGCTCCACTCCCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTaga              <  1:120051/147‑1 (MQ=255)
                                                                                                                                         aagaagaaATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGttt  >  2:395575/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GTTCGGATAGTTAACAGAAACAGGTTCTCGTGTATTATTTCATCCTAAGTAAAACAACGGAGAACCTGCAATGGCACAACCTGCCGCTATTATTCGTATAAAGAACCTTCGTTTGCGTACGTTTATCGGAATTAAGGAAGAAGAAATTAACAACCGTCAGGATATTGTTATCAATGTGACGATCCACTACCCCGCCGATAAAGCGCGCACTAGCGAAGATATCAACGATGCGCTGAATTATCGCACCGTAACGAAAAACATTATTCAGCATGTAGAGAATAACCGTTT  >  NC_000913/2421255‑2421542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑