Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,029,9590AC45.5% 66.8 / 10.7 44C325G (TGT→GGT) ygfTputative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base A (19/5);  new base C (0/20);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 3.34e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTTATCGAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTG  >  NC_000913/3029817‑3030103
                                                                                                                                               |                                                                                                                                                
gCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTTATCGAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAAcc                                                                                                                                          >  2:477070/1‑151 (MQ=255)
    tGAATATCTCTCGCCGCTGGCTTAATACCGTTTTATCGAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAAccc                                                                                                                                         <  2:559744/148‑1 (MQ=255)
             ctcGCCGCTGGCTTAATACCGTTTTATCGAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCa                                                                                                                               <  2:506862/149‑1 (MQ=255)
                   gcTGGCTTAATACCGTTTTATCGAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAAt                                                                                                                       <  2:420772/151‑1 (MQ=255)
                   gcTGGCTTAATACCGTTTTATCGAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAAt                                                                                                                       >  1:596067/1‑151 (MQ=255)
                          tAATACCGTTTTATCGAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCg                                                                                                                  <  1:448793/149‑1 (MQ=255)
                                       tCGAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTAc                                                                                                   >  2:236848/1‑151 (MQ=255)
                                         gAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACgg                                                                                                 >  2:472446/1‑151 (MQ=255)
                                         cgttttGAAAGGGGGGAATCCAAAAAGCCAGCACCCCGCAATTTTCGGGATGGGGATCAAAAACATCGACCTGACCCCCGGCGCGCCCAAGAATATCAGCACCCCCTACCCCGGCGGGCCCGGCGCCAATCACCGCCACTTTTTCGCTa                                                                                                    <  1:268907/146‑1 (MQ=255)
                                          aGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGCCATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCGGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACgg                                                                                                 >  1:91440/1‑150 (MQ=255)
                                            tttGAAA‑GGAGGAATGCAAAAAGTCACCATCCCGCCAATTTCTGGATGGCAATAAAAGACATCGACCTGAACCCCTGCGCGCGCCAGAATATCAGCACCCCCTACCCCGGCAGGCCCAGCGCAAATCACCGCCACTTTTTCGCTACgggg                                                                                               <  2:87185/150‑1 (MQ=255)
                                            tttAAAA‑GGAGGAAGCCAAAAAGCCAGCACCCCGCAATTTTCGGGATGGCAATAAAAGACACCGCCCTGACCCCCGGCGCGCGCAAGAATATCAGCACCCCCAACCCCGGCAGGCCCGGCCCCAATCACCGCCACTTTTTCGCTACgggg                                                                                               <  1:36097/150‑1 (MQ=255)
                                            tttAAAA‑GGAGAAATCCAAAAAGCCAGCATCCCCCCAATTTTTGGATGGCAATAAAACACATCGCCCTGACCTCCGGCGCGCGCCAAAATAACACCCCCCCCAACCCCTGCAGCCCCGGCCCAAATCACCGCCACTTTTTCGCTACg                                                                                                  <  1:585075/147‑1 (MQ=255)
                                             ttGAAA‑GGAGGAATGCAAAAAGTCAGCACCCCCCCATTTTCTGGATGGCGATCAAAGACATCGCCCTGAACTCCTGCGCCCGCAAGAATATCAGCACCCCCTACCCCGGCAGGCCCGGCGCCAATCACCGCCACTTTTTCGCTACGGGGa                                                                                              <  2:22939/150‑1 (MQ=255)
                                              tGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGaac                                                                                            >  2:280317/1‑151 (MQ=255)
                                               taaaagaaaaaaTGCCAAAAGACAGCTTCCCGCAATTTTCTGGATGGCGATCAAAAACTCCCCCCTGAACCCCTCCGCGCCCCAGATTATCAGCCCCCCCAACCCCTGCAGGCCCAGCGCAAATCACCGCCACTTTTTCGCTACGGGGaa                                                                                             <  2:101629/140‑1 (MQ=255)
                                                  a‑ggaggAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACt                                                                                        >  2:426006/1‑151 (MQ=255)
                                                               aaagctCAGCACCCCCCCATTTTCGGGATGGCAATCAAACCCCCCCCCCTGACCCCCTCCCCCCGCCAAAATATCACCCCCCCCCACCCCTGCAGCCCCACCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGa                                                                                 <  1:26575/141‑1 (MQ=255)
                                                                aaaGTCAGCATCCCGCCAATTTCTGGAGGGCGAAAAAAGCCACCGACCTAACCCCCGGCGCGCGCCAGAATATCACCCCCCCCAACCCCGGCGGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCgg                                                                           <  2:567739/151‑1 (MQ=255)
                                                                    tCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACg                                                                       >  2:38754/1‑151 (MQ=255)
                                                                     cAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGc                                                                      >  1:664697/1‑151 (MQ=255)
                                                                          cccccccAATTTCGGGAGGGCGATAAAAGCCACCGCCCGGACCCCCGGCGCGCTCAGAAATAGCTGCACCCCGTACCCGTGCGGCCCCGGCGCCAGGCGCCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCa                                                                    <  1:176178/143‑1 (MQ=255)
                                                                            ccGCAATTTTCGGGATGGCAATCAAAGACACCGCCCGAAACTCCTGCGCGCGCCAGAATACCACCCCCCCCTACCCCACCAGGCCCCGCGCCATTCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGAc                                                                        <  1:437152/142‑1 (MQ=255)
                                                                                cAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGc                                                           >  2:552619/1‑151 (MQ=255)
                                                                                   tttCTGGATGGCAAACAAACACCCCGACCTAACCCCCGGCGCGCCCCAGAAAACCGACCCCCCCCACCCCCGCGGCCCCAGCGCAAATCCCCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATc                                                             <  2:714707/146‑1 (MQ=255)
                                                                                   tttCTGGAGGGCGATAAAAGACATCGACCTGACCCCCTGCGCGCGCAAGAATATCAGCACCCCCTACCCCGGCAGGCCCAGCGCAAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCg                                                            <  2:450127/147‑1 (MQ=255)
                                                                                       tGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGcgcg                                                    >  2:491344/1‑151 (MQ=255)
                                                                                           tGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTAt                                                >  2:554048/1‑151 (MQ=255)
                                                                                                  cAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGAt                                         >  2:5444/1‑151 (MQ=255)
                                                                                                   aaaGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATg                                        >  1:265218/1‑151 (MQ=255)
                                                                                                   aaaGACACCGCCCTAACCCCCGGCCCGCCCAAAAATACCACCCCCCCCAACCCCGGCAGCCCCAGCGCAAACCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCg                                              <  1:718681/145‑1 (MQ=255)
                                                                                                   aaaGACACCCCCCTGACCCCCTGCGCGCGCCAGAATCTCACCCCCCCCAACCCCTGCAGCCCCAGCGCCAACCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGa                                          <  1:201840/149‑1 (MQ=255)
                                                                                                              ccggacccccggcccgcgcAAGAAAACCAGCCCCCCCAACCCCTGCGGGCCCGGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGc                                    <  1:236797/130‑1 (MQ=255)
                                                                                                                  acccccTGCGCCCGCCAAAATATCACCCCCCCCTACCCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTa                         <  2:572304/147‑1 (MQ=255)
                                                                                                                            cgcgcAAAATTACCAGCACCCCCAAACCCGGCGGGCCCAGCGCAAACCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAgaga               <  1:675867/151‑1 (MQ=255)
                                                                                                                             gcgcCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGAc              >  2:94956/1‑151 (MQ=255)
                                                                                                                               gcCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTg            >  2:385216/1‑151 (MQ=255)
                                                                                                                                caaaaaTATCAGCCCCCCCAACCCCGGCAGGCCCAGCGCAAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTg            <  2:98066/146‑1 (MQ=255)
                                                                                                                                  aaaaTACCAGCACACCCTACCCCGGCAGCCCCAGCCCAAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTg            <  1:201186/146‑1 (MQ=255)
                                                                                                                                  aGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTgcgc         >  1:184427/1‑151 (MQ=255)
                                                                                                                                     atatCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCg      >  2:427375/1‑151 (MQ=255)
                                                                                                                                       atCAGCACCCCCTACCCCGGCAGCCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAg    <  2:34444/151‑1 (MQ=255)
                                                                                                                                       atCACCACCCCCTACCCCGACAGCCCCAGCCCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGa     <  1:402120/150‑1 (MQ=255)
                                                                                                                                          aGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCAAAAGCCAGCGCGGCATCGGAGATGTAGCGCTTCAGATTACCGATAGAGACTGCGCCCGAGTg  >  2:368305/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GCGGTGAATATCTCTCGCCGCTGGCTTAATACCGTTTTATCGAGTTTGAAA‑GGAGGAATGCCAAAAGTCAGCATACCGCCAATTTCTGGATGGCGATCAAAGACATCGACCTGAACTCCTGCGCGCGCCAGAATATCAGCACACCCTAACCCTGCAGGCCCAGCGCCAATCACCGCCACTTTTTCGCTACGGGGAACAACTTTGCTGACATCGGGACGCCAGCCCATCGCCAGCGCGGTATCGGTGATGTAGCGTTCCAGATTACCGATAGAGACTGCGCCCGAGTG  >  NC_000913/3029817‑3030103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑