Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,2870GC44.7% 54.5 / 16.1 38intergenic (+93/+136)yhdZ/rrfFputative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base G (2/19);  new base C (17/0);  total (19/19)
Fisher's exact test for biased strand distribution p-value = 1.19e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCC  >  NC_000913/3423142‑3423437
                                                                                                                                                 |                                                                                                                                                       
cgcATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAcc                                                                                                                                                    <  2:218253/151‑1 (MQ=255)
 gcATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGcccccccccc                                                                                                                                                   >  2:444037/1‑151 (MQ=255)
     ccTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTcc                                                                                                                                                <  2:564221/150‑1 (MQ=255)
       tAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTGCTGCCCCCCCCCCCCCcgg                                                                                                                                             >  1:486864/1‑151 (MQ=255)
            cAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGCcccccccccccc                                                                                                                                                >  1:507576/1‑143 (MQ=255)
                       aGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTCCTGCCCCCCACCCCCCCGGCCGTTGCTTTGCaa                                                                                                                               >  2:628141/1‑149 (MQ=255)
                       aGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACg                                                                                                                             <  1:495946/151‑1 (MQ=255)
                            aTTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCCCCCTCCCGGCCGTTGCTTTGCCAAGtt                                                                                                                           >  1:345386/1‑148 (MQ=255)
                             tttttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCTCCCCCCCCCCCCCCGGCCGGTTCCTCCCAACCTTCa                                                                                                                         >  2:727846/1‑149 (MQ=25)
                                ttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATc                                                                                                                     <  1:107325/150‑1 (MQ=255)
                                        ggTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCTCCGGGCCGTTGTTTTCCAACGTTCAAATCCGCcccc                                                                                                              >  2:195828/1‑149 (MQ=21)
                                            aTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCACCCCCCCGGCCGTTGCTTCGCAAAGTTCAAATCCCCCCCccgcgg                                                                                                         >  2:24205/1‑150 (MQ=21)
                                             tCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTCCCCCCCCCCCCCCGGCCGTTGCTTCGCAAAGTTTAAAACCGCTCCccgcgg                                                                                                         >  2:449720/1‑149 (MQ=21)
                                               cATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAttt                                                                                                     <  1:567323/151‑1 (MQ=255)
                                               cATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCGGCCGTTGCTTCGCAACGTTTAAATCCGCCCCccgcggggtt                                                                                                     >  1:132443/1‑147 (MQ=255)
                                                       gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTCCCGCCCCCCCCCCCCCCGGCCGGTTCTTTGCAACGTTCAAAACCCCCCCCCGCGGGTTTTTCCTAc                                                                                              >  1:64373/1‑150 (MQ=255)
                                                           gTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCCTCCCCCCACCCCCCCGGCCGGTGCTTCGCAAAGTTCAAATCCGCCCCCCGCGGGTTTTTCCTACTCggg                                                                                         >  2:378842/1‑151 (MQ=18)
                                                                 tCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGTTGCTTTGCAACGTTTAAATCCCCTCCCCGCGGGGTTTTCCTACTCCGgaggg                                                                                     >  2:70080/1‑147 (MQ=17)
                                                                              aGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGac‑aaac                                                                     <  2:687647/151‑1 (MQ=255)
                                                                              aGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCTCCCGGGCGGTGCTTCGCAAAGATTAAATCCCCCCCCCGCGGGGTTTTCCTACTCCGGggaggggttccccacaaa                                                                       >  2:439892/1‑136 (MQ=9)
                                                                                gCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGac‑aaacaa                                                                   <  1:152303/151‑1 (MQ=255)
                                                                                      cTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTCCCCCCCCCCCCCCGGCCCTTGCTTCGCAACGTTCAAAACCCCCCCCCGCGGGTTTTTCCTACTCCGGGGAGGGTTTACCCAC‑CAACAAAAGa                                                               >  2:103838/1‑149 (MQ=14)
                                                                                       tttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGCCCGCCCCCCCCCCGGCCGTTGCTTTCCCACCTTCAAATCCGCCCCCCGCCGGTTTTTTCTCCTCCGGGGAGGGGTTACCCAC‑AAACAACAGATa                                                             >  1:338708/1‑150 (MQ=255)
                                                                                        ttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATa                                                             <  1:189679/149‑1 (MQ=255)
                                                                                           ggATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCACCCCCCCGGCCGTTGCTTCGCAACGTTTAAATCCCCTCCCCGCGGGTTTTTCCTACTCCGGGGAGGGTTTACCCAC‑AAACAAAAGATaaaa                                                          >  2:665933/1‑149 (MQ=255)
                                                                                            gATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACaa                                                       <  1:347511/151‑1 (MQ=255)
                                                                                             aTGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCGGCCCTTGCCTCCCAACCTTCCAATCCCCCCCCCGCCGCCTTTTCCTTCCCCGGGGAGGGGTCCCCCAC‑CAACAAAAGATAaaaaaa                                                       >  1:178625/1‑150 (MQ=9)
                                                                                                 ccTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCGGGCCGTTGCTTCGCAACCTTCAAATCCCCCCCCGGCGGGTTTTTCCTACCCGGGGGGGGGTTCACCGACAAAAAAACAAAAAAAAAAAAAg                                                    >  2:421273/1‑150 (MQ=14)
                                                                                                       gCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCGGCCGGGTCTTTGCCACCTTTAAATCCCCCCCCCGCGGGGTTGTCCTCCTCGGGGGAGGGGTCACCGCC‑AAACAACCGACAAAAAAAAAAGccccggc                                            >  1:235306/1‑147 (MQ=255)
                                                                                                                gatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTg                                   <  1:619138/151‑1 (MQ=38)
                                                                                                                gatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTg                                   <  2:23567/151‑1 (MQ=38)
                                                                                                                 atGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGa                                  <  2:687220/151‑1 (MQ=38)
                                                                                                                           gTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGtt                        <  2:453000/151‑1 (MQ=38)
                                                                                                                           gTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGtt                        <  2:673860/151‑1 (MQ=38)
                                                                                                                              tATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGtttt                      <  2:59027/150‑1 (MQ=38)
                                                                                                                              tATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGtttt                      <  1:429284/150‑1 (MQ=38)
                                                                                                                                 ggcgggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTAtt                   <  2:132471/150‑1 (MQ=38)
                                                                                                                                 ggcgggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTAtt                   <  2:702837/150‑1 (MQ=38)
                                                                                                                                            tGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGc        >  1:88019/1‑150 (MQ=40)
                                                                                                                                             gCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCa       <  1:702667/150‑1 (MQ=40)
                                                                                                                                                 gCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTcc  <  2:486987/151‑1 (MQ=40)
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CGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGAC‑AAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCC  >  NC_000913/3423142‑3423437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑