Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	57.6%	36.1 / 48.4	33	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (14/0);  new base C (0/19);  total (14/19)
Fisher's exact test for biased strand distribution p-value = 1.22e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-08

ACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACT  >  NC_000913/3443145‑3443420                                                                                                                                     |                                                                                                                                                acaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACtt                                                                                                                               >  1:459713/1‑151 (MQ=255) acaTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACtt                                                                                                                               >  2:719920/1‑151 (MQ=255)  caTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACttt                                                                                                                              >  2:266768/1‑151 (MQ=255)      aaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGc                                                                                                                          >  1:78804/1‑151 (MQ=255)       aGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCg                                                                                                                         >  2:713531/1‑151 (MQ=255)          ggtcccccggactaaaaaTACAGAGAAATTGTTGTCAGCCAGTCCCAACCGGTCCCCCCCCCGACCCATGACGCGATGGTCGCCGGAAAAAAAATAACACGGGAAGGAAAGATGGGCGGAATCCCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                      <  1:36787/136‑1 (MQ=255)                   ggCTACAAATACAGCGAATTTTTTGCCAGCCAGTCCCAACCATTCCCCCCCCCAACCCAGGACGCGATGGCCGCGGGAAAAAAAATAATACTGGGGGCGAAGTTGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAAtg                                                                                                                  <  1:647700/146‑1 (MQ=255)                        cAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                         >  2:369584/1‑150 (MQ=255)                                   aaaTTGTTGTCAGCCATTCCCAACCAGTCCCCCCCCCGACCCATGACGCGATGGTCGCCGGAAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgca                                                                                                     <  2:461520/143‑1 (MQ=255)                                    aaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                             >  1:642121/1‑150 (MQ=255)                                         ttgtCACCCAGTCCCAACCGGTCCCCCCCCCGACCCATGACGGGATGGTCGCGGGAAACAAAAAAATACGGGAAGCGAAGATTGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                          <  2:635263/148‑1 (MQ=255)                                                ccattcccACCCAGTCCCCCCCCCGACCCATGACGGGATGGTCGCGGGGAACAAAATAATACTGGAAGCGAAGATGGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgacg                                                                                       <  2:696399/138‑1 (MQ=255)                                                      caacccggtcccccccccAACCCAGGAGGCGATGGTCGCGGGAAAAAAAATAATACGGGAAGCAAAGTTTGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                           <  2:486174/137‑1 (MQ=255)                                                      caaaCCAGTACCGCCCCCAACCCAGGACGCGAGGTCCGCGGGGAAAAAAAAATTAGTGGAGGAAAAAATGGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                   <  1:602049/140‑1 (MQ=255)                                                       cAACCAGAACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTACCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                          >  2:291532/1‑150 (MQ=255)                                                                            cATGCCGCGAGGGCCGCCGGGAACAGAATAATACGGGAAGCGAAGTTTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                    <  2:396243/151‑1 (MQ=255)                                                                               gACGGGATGGTCGCGGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                 <  1:690301/151‑1 (MQ=255)                                                                                  gcgGTGGTCGCCGGAAACAGAATATTACTGGAGGTGAGGAGTGCGGGGATACCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACGCGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGtt                                              <  1:10928/151‑1 (MQ=255)                                                                                    gATGGGCGCCGACAACAGAATAAGACGGGAAGCGAATATTGCCGGCATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGa                                            <  1:371159/151‑1 (MQ=255)                                                                                          cGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGc                                      >  1:231550/1‑151 (MQ=255)                                                                                            cgggaaaCAAAAAAATACTGGAAGCGAGGATGGCGGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCa                                         <  2:337201/141‑1 (MQ=255)                                                                                                    aaaataatACTGAAAGCGAATATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                             <  2:103179/148‑1 (MQ=255)                                                                                                     gAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATac                           >  1:115547/1‑151 (MQ=255)                                                                                                      aataatACTGGAAGGGAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                          <  2:231600/151‑1 (MQ=255)                                                                                                      aataatACGGAAAGCGAATATGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                          <  1:294295/151‑1 (MQ=255)                                                                                                        taataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                        >  2:407683/1‑151 (MQ=255)                                                                                                         aataCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                        >  2:152458/1‑150 (MQ=255)                                                                                                          atcgaggAAGGGAATATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                       <  2:74142/145‑1 (MQ=255)                                                                                                               ggAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAAc                 >  1:626824/1‑151 (MQ=255)                                                                                                                      aaGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt               <  2:209257/146‑1 (MQ=255)                                                                                                                            gcgggtaTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCaaa      <  2:464083/143‑1 (MQ=255)                                                                                                                            gCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat     <  2:21588/150‑1 (MQ=255)                                                                                                                              cGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact  >  2:486643/1‑151 (MQ=255)                                                                                                                                     |                                                                                                                                                ACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACT  >  NC_000913/3443145‑3443420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑