| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,862,667 | 0 | G | A | 46.0% | 21.9 / 15.2 | 37 | pseudogene (937/1617 nt) | glvC | pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component |
| Reads supporting (aligned to +/- strand): ref base G (12/8); new base A (0/17); total (12/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.13e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
CATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCG > NC_000913/3862518‑3862809 | cATGTTGCCCACCACAACAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGGT‑AAAGTTGCggg > 1:140788/1‑150 (MQ=255) ccaccaCACCAAAGAGATACATTACAGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAAGGCTCGGGCATCAGCa > 1:400134/1‑150 (MQ=255) aaGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGTAACGGTGAAAGGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AGGGTTGCGGGAATCAGCAAGCCCGCCACtt < 1:180785/151‑1 (MQ=255) cATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGGTGCGGGGATCAGCAAGCCCGCCACTTTTTccc > 2:514954/1‑149 (MQ=255) aTTAGGGTCGACATGGAGCCCCCCAGCACGGCGTGTCCCGAAAACAGCAACGGTGAAATGAACAGGAAGTTAAATCCCGGC‑GG‑TTCGTTATTCCCCACCAGCAGGGCGGTT‑AAGGTTGGGGGAATCAGCAAGCCCGCCACTTTTACCCGa < 1:643771/150‑1 (MQ=255) ttACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGGTGCGGGGATCAGCAAACCCCCCCCTTTTACCCGGtt > 2:573870/1‑151 (MQ=255) tACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGGTGCGGGGATCAGCAAGCCCCCCCCTTTTACCCGAttt > 2:696313/1‑151 (MQ=255) ggTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGGTGCGGGGAACAGCAAGCCCCCCACTTTTACCCGAtttt > 2:364299/1‑149 (MQ=255) cGACATGGAGGCCGCCAGCACGGCGTGTCCCGCAAACAGCAACGGTAAAATGAACAGGAAGGAAAATTCCAGC‑GT‑TTGGGTAATCCCCACCAGCATGGGGGTT‑AAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGt < 2:9110/151‑1 (MQ=255) ttGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGGTGCGGGGAACAGCAAGCCCGCCACTTTTTCCCCATTTTTCGGTGCgg > 2:588684/1‑150 (MQ=255) ccgccAGCACGGGGTGTCCCGCAAACAGCAACGGTAAAATGAACAGGAAGGTAAATTCCGGC‑GG‑TTCGGTAATTCCCCCCAGCAGGGCGGTA‑AAGGTTGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGaa < 2:209664/151‑1 (MQ=255) ccAGCGCGGCGTGTCCCGAAAACAGCAACGGAAAAAGGAACAGGAAGGTAAATTCCAGG‑GG‑TTCGGTAATCCCCCCCAGCATGGCGGTT‑AAGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTa < 1:724926/151‑1 (MQ=255) aGCACGGCGTGTCCCGCAAACAGAAGCGGTAAAAGGAACAGGAAGGTAAATTCCAGG‑GT‑TTCGGTAATCCCCCCCAGCATGGCGGTT‑AGGGTGGGGGGAATCAACAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTa < 1:34840/149‑1 (MQ=255) aGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGGTGCGGGAATCAACAAACCCCCCCCTTTTACCCGATTTTCCGGTGCGGCAGGGaa > 2:528672/1‑146 (MQ=255) ccacAAACAGCAACGGTGAAATAAACGGGAAGTAAAATCCCGGG‑GG‑TTCGTAAATCCCCCCCGGCATGGCGGTT‑AAGGTGGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGaa < 2:227195/146‑1 (MQ=255) ccGAAAAAAGCAACGGAAAAAGGAACAGGAAGGAAAATCCCAGC‑GT‑TGGGGAAATCCCCACCAGAGGGGCGGTT‑AAGGGTGCGGAATTAAAAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGaaa < 1:644781/150‑1 (MQ=255) aaaCAGCAAGGGTAAAATAAACAGGAAGGTAAATTCCAGG‑GT‑TTCGGAAATCCCCACCAGCATGGGGGTA‑AGGGTGGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGcc < 2:47290/150‑1 (MQ=255) aaaCAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGGT‑AAGGGTGCGGGGATCACCAAACCCCCCACCTTTACCCGATTTTCCGGTGCGGGAGGGAAGCACATCGCTAAAAAAAAGccc > 1:540059/1‑151 (MQ=255) aaaCAGCAACGGTAAAATAAACAGGAAGGAAAATTCCGGC‑GG‑TTCGGTAATCCCCCCCAGCAGGGCGGTT‑AAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAAt < 2:399581/147‑1 (MQ=255) aaCAGCAACGGTAAAAAAACGAGGAGGGTAAATCCCAGC‑‑G‑TTTGGTAAACCCCCCCGAGAGGGGGGTTAAAGGTGGCGGAAATCAACACCCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGaaa < 1:497407/145‑1 (MQ=255) aGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGGTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACgg > 2:169900/1‑151 (MQ=255) gCAACGGTGAAAAGAAAAGGAAGGTAAATTCCAGC‑GG‑TTGGGTAATTCCCACCAGCATGGCGGTT‑AAGGTGGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACgg < 1:470033/150‑1 (MQ=255) aaaTGAACAGGAAGGTAAA‑GCCGGCGGG‑TTGGATAATCCCCCCAAGCAGGGGGGTT‑AAGGTGGGGGAAATCAACAGCCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAg < 1:588931/149‑1 (MQ=255) aaGGTAAATTCCGGG‑GG‑TTCGATAATGCCCACCAGCAGGGGGGTT‑AAGGTGGGGGAAAGCAACAACCCCGCCACTGTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAg < 1:454564/147‑1 (MQ=255) aGGTAAAATCCCGG‑GGTTTCGGTAATCCCCCCCAGCATGGGGTTT‑AAGGTGGGGGAAAACAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTa < 1:307318/150‑1 (MQ=255) aaaTTCCAGC‑GT‑TTCGGTAATCCCCACCAGCATGGCGGTT‑AAGGTGGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTg < 2:234928/150‑1 (MQ=255) aaaTTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGGTGCGGGAATCAGCAAGACCGCCACTTTTTCCCGATTTTCCGGTGGGGCAGTGAAGTACATCGCCAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTAcc > 1:609980/1‑147 (MQ=255) ttCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGGT‑AAAGTTTCGGGGATCAGCAAGCCCGCCCCCTTTTCCCGATTTTCCGGTGCGGCCGTGAAGTTAATCGCCAAAGAAATGCCCAAGGCCACCAAGATTTTTTTGTTAACGTg > 2:311602/1‑147 (MQ=255) ccAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGGTGCGGGAATCAGCAAACCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCCCGGCACCAAAGATTTTTGAGTTACCGTGCAggg > 2:551979/1‑150 (MQ=255) ccAGC‑GG‑TGCGTTAATCCCCCCCAGCAGGGCGGTT‑AAGGTTGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAggg < 2:227481/150‑1 (MQ=255) ttggttAATCCCCCCCAGCATGGGGGTT‑AAGGTGGGGGAAATCAGAAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAAcc < 2:336740/145‑1 (MQ=255) ggTAATTCCCACCAGCAGGGCGGTT‑AGGGTGGCGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCg < 2:548915/148‑1 (MQ=255) aacccccACCAGCAGGGCGGTA‑AGGGGTGGGGAAACCGCCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTc < 1:101829/146‑1 (MQ=255) tcccccccAGCAGGGCGGTT‑AAGGTTGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTcc < 2:591078/147‑1 (MQ=255) ccccccAGCAGGGCGTTT‑AGGGTGGGGGAAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCgg < 1:356879/149‑1 (MQ=255) agggTTGGGGAAACCAGCAGGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCgg < 1:707586/147‑1 (MQ=255) ggTTGCGGAAATCAGAAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCg < 2:197327/151‑1 (MQ=255) ggTGGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCg < 2:653646/151‑1 (MQ=255) | CATGTTGCCCACCACACCAAAGAGATACATTACGGTCGACATTGAGGCCGCCAGCACGGCGTGTACCGCAAACAGCAACGGTGAAATGAACAGGAAGGTAAATTCCAGC‑GG‑TTCGGTAATTCCCACCAGCATGGCGGTT‑AAGGTTGCGGGAATCAGCAAGCCCGCCACTTTTACCCGATTTTCCGGTGCGGCAGTGAAGTACATCGCTAAAGAAATGCCCACGGCACCAAAGATTTTTGAGTTACCGTGCAGGGCAAAACCGCCTTCCGGGAACAACGATTTCAGCGGCTCG > NC_000913/3862518‑3862809 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |