Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913907,6020AC51.7% 34.9 / 46.5 29G227G (GGT→GGG) ybjTputative NAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (13/1);  new base C (0/15);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 2.06e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.77e-06

CATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCA  >  NC_000913/907466‑907737
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catcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCAt                                                                                                                           >  2:533726/1‑151 (MQ=255)
  cccGCCAGCAGATCGTGTTTCAGCCCCTGAATCACCCCCCGGGCGGTGGGGGGGGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGGGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTCCCGCTCACCGCCATaa                                                                                                                         <  1:224764/150‑1 (MQ=255)
               cGTGTTGCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTg                                                                                                            >  1:202616/1‑151 (MQ=255)
                                     cGCCCTGGTGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGAGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACctct                                                                                      >  2:272469/1‑151 (MQ=255)
                                     cGCCCGGGGGGTGGGGGGCGTTACGGAATTAATCAAATTAAAAACCCCCCCCGAACCCCGGGGGGGGGGGGGGGGGATGGGGATCACCCAGCGGCCCTTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACctc                                                                                       <  1:221837/150‑1 (MQ=255)
                                       cccGGGGGGGGGGGGGGGGTACGGAAGTAACCACATGGAAAACCCACCCCAAAACCCAGGGGGGGGGGGGGGGGGTGGGGACCAACCGGGGGCGCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTgg                                                                                    <  1:270857/151‑1 (MQ=255)
                                          tGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTcc                                                                                 >  2:557636/1‑151 (MQ=255)
                                                      ggggTACGGAAGAAATCACATTGAGAACCCCCACCAAAACCCAGGGGGGGGGGAGGGGGGTGGGGATCACCCAGGGGCGCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGaa                                                                     <  1:631586/149‑1 (MQ=255)
                                                        ggTACGGAAGAAATCCCATTGAAAAACCCCCCCGAAACCCGGGGGGTGGGGAGGGGGAGGGGAATCACCCGGCGGCGCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGa                                                                   <  1:465860/151‑1 (MQ=255)
                                                            cGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCg                                                               >  1:276897/1‑151 (MQ=255)
                                                              gAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGgt                                                             >  2:471645/1‑151 (MQ=255)
                                                                  tAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTc                                                         >  2:86127/1‑151 (MQ=255)
                                                                           tGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCgg                                                >  1:352164/1‑151 (MQ=255)
                                                                                  ccACCCCAAAACCCGGGGGGGGGGGGGGGGGAGGGGGATCACCCTGCGGCTTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGgatgat                                           <  1:474663/149‑1 (MQ=255)
                                                                                    acacCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTa                                        >  1:414111/1‑150 (MQ=255)
                                                                                           accccgggggggggggAGGGGGGTGGAGATCACCCGGCGGCCCTCCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACg                                  <  1:69247/141‑1 (MQ=255)
                                                                                            aTCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCCGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCa                                >  2:711987/1‑150 (MQ=255)
                                                                                              ccAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAcc                             >  1:726009/1‑151 (MQ=255)
                                                                                              ccAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAcc                             >  2:326191/1‑151 (MQ=255)
                                                                                                 ggggggggggggggggAGGGGGATCAACCGGCGTCGCTTCCCTCCAACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAAcc                             <  2:678356/146‑1 (MQ=255)
                                                                                                      gggggggggggGTGGGGATAAACCAGCGGCCCTTCCCCCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTg                     <  1:621529/150‑1 (MQ=255)
                                                                                                       ggggagggggagGGGGATCACCCAGCGGCGCTTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTgc                    <  1:305820/151‑1 (MQ=255)
                                                                                                                   ggggacCAACCAGCGGCCCTCCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTcc        <  1:291858/151‑1 (MQ=255)
                                                                                                                   gggaaccacccggcggcgCTCCCCCCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGttt           <  1:233581/136‑1 (MQ=255)
                                                                                                                    gggatCACCCAGCGGCGTTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCa       <  2:416975/151‑1 (MQ=255)
                                                                                                                     ggatCAACCAGCGGCGCTTACCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAg      <  2:143707/151‑1 (MQ=255)
                                                                                                                         cAACCGGCGGCGCTTCCCGCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAgc     <  1:222389/148‑1 (MQ=255)
                                                                                                                          acccAGCGGCCCTTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCa  <  2:490080/148‑1 (MQ=255)
                                                                                                                          aaCCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCa  >  2:131279/1‑150 (MQ=255)
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CATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGGATCAACCAGCGGCGCTTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTTTCCAGCGCA  >  NC_000913/907466‑907737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑