Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC52.3% 42.3 / 64.4 44T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (16/5);  new base C (0/23);  total (16/28)
Fisher's exact test for biased strand distribution p-value = 4.88e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.54e-06

GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCG  >  NC_000913/1376356‑1376637
                                                                                                                                                      |                                                                                                                                    
gCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAacac                                                                                                                                     >  1:312940/1‑151 (MQ=255)
  ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACAccc                                                                                                                                   >  1:332864/1‑151 (MQ=255)
     ccgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTc                                                                                                                                 <  2:578258/150‑1 (MQ=255)
      cgccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGc                                                                                                                               >  1:564397/1‑151 (MQ=255)
                 tCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCccgcc                                                                                                                    >  1:587251/1‑151 (MQ=255)
                         tAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAt                                                                                                             >  2:61276/1‑150 (MQ=255)
                               ggACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGa                                                                                                       >  1:672657/1‑150 (MQ=255)
                                gACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGaa                                                                                                      >  1:95293/1‑150 (MQ=255)
                                  tggCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAAc                                                                                                   >  1:159502/2‑151 (MQ=255)
                                        ccGAAAAATGGGGGTTGATTCCCGGCGCGTCATGGAACAGGTCGCCGCGCTTACCGGAACCGTGGTGTATCTCGACCCTTTAAC‑CCGCTACCAGAATCATATAAACACCCCCCTCGCCGAGGCCCGCCGTTACATCGTCGAAAACGAt                                                                                                <  1:147502/148‑1 (MQ=255)
                                         cgaaaaaTGGGGAGTGATTCCCTGCGCGTACTGGAACAGGGCCCCGCGCGTCCCGGACCCGTGGTGTACCTCGAACCGTAAAC‑CCGCTATCAGAATAATATGACAACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGAt                                                                                                <  1:128013/144‑1 (MQ=255)
                                          aaaaaaTGGTGATTGTTCCCCTGGTCTTCCGGGACCGGTCCGCCGCGCGTACCGAAACCGTGTTTTATCTCAACCCTTTAAC‑CCGCTTAAAGGATCATATGACCAACCCCCGCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                           <  1:604460/149‑1 (MQ=255)
                                           cAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                           >  1:295128/1‑150 (MQ=255)
                                            aaatgggTGAGTGTTTCCCGGCGCGTACGGAAACGGGTCGCCGCGCTTACGGGACCGGTGGTGTATCTCGACCCTTTAAC‑CCGCTATCAGGATAATATGATAAACACCCCCCCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                           <  1:588496/144‑1 (MQ=255)
                                            aaaaTGGTGATTGATCCCCTGCGCGTACTGAAACAGGTCGCCGCGCGTCCGGGACCCGTGGTGTATCTCGACCCTTAAAC‑CCGCTATAAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGAt                                                                                                <  2:233730/144‑1 (MQ=255)
                                            aaaaTGGGGGTTGTTCCCCTGCGCGTAGTGAAACAGGTCGCCGCGCGTCCGGAAACGGTGTTTTATCGCGAACTTTAAAC‑CCTCTTTCGGGTTCTTTTGACAACCCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTaaa                                                                                          <  2:603771/150‑1 (MQ=255)
                                                tGGTGAGTGATTCCCTGCGCGTCCTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGACCCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                      <  1:528466/150‑1 (MQ=255)
                                                  gggggtgttccccTGCGCGTCCTGAAACAGGTCGCCGCGCGTACCGGAACGGTGGTGTATCTCGAACCGTAAAC‑CCGCTATCAGGATAATATGAACAACCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                   <  2:672582/141‑1 (MQ=255)
                                                    gagtgtTTCCCTGGGGGTCTTGAAACGGTTCGCCGCGCGTACCGGACCGGGGGTTTACCTCGACCCTTAAAC‑CCGTTACCGGGTTAATATAATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACa                                                                                  <  1:232001/150‑1 (MQ=255)
                                                        gaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                              >  1:231357/1‑150 (MQ=255)
                                                          ttCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                           >  2:608618/1‑151 (MQ=255)
                                                           tCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGAACTTAAACATGTACAGATTATCg                                                                          >  1:539469/1‑151 (MQ=255)
                                                            cccTGCGTTTCCTGGAACGGCCCGCCGCGCGTCCCGAACCGGTGGTTTATCTCGACCCTTAAAC‑CCGTTATAAGAATAATATAACAAACCCCCTCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCg                                                                          <  2:478543/150‑1 (MQ=255)
                                                             ccTGCGCGTGCTGGAACAGGTCGCCGCGCGTGCCGGAACGGTGGTTTATCTCGAACCTTAAAA‑CCGATATCAGGATAATATGATCAACACCCTCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTa                                                                             <  1:512973/146‑1 (MQ=255)
                                                                gcgcGTACTGAAACGGGTCGCCGCGCGTCCGGGAACGGTGGTGTATCCCAAGCCTTAAAC‑CCGCTATCAGGATAATATGAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAt                                                                     <  2:633714/151‑1 (MQ=255)
                                                                    gTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCt                                                                 >  1:80690/1‑151 (MQ=255)
                                                                     tACTGAAACGGGTCGCCGCGCGTCCCGGACCGGTGGTGTATC‑CCAACCCTTAAACCCGCTATCAGGATCATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAttt                                                                   <  2:243095/148‑1 (MQ=255)
                                                                           accAGGCCGCGGGGCGTACCGGACCCGTGGTGTATC‑CGAACCCTTAAACCCGTTACCAGGATAAAATAATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATca                                                             <  1:325328/146‑1 (MQ=255)
                                                                                             ccGGAACGGTGGTTTATCTCGACCCTTAAAC‑CCGCTATCAGGATCATATGACCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                          <  2:725782/149‑1 (MQ=255)
                                                                                             ccGGAACGGTGGTGTATCTCGACCCGTTAAC‑CCGCTATCAGGATCATATGATCAACCCCCCCGCCAATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGaagaag                                            <  2:221238/147‑1 (MQ=255)
                                                                                               gaaaCGGTTGTGTATTTGGACCCTTAAAC‑CCGCTATGAGGATAAAAGGACAAACCCCCCGGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAc                                       <  1:108325/148‑1 (MQ=255)
                                                                                               gaaaCCGTGGTTTATCTCAACCCGTTAAC‑CCGCTATCAGGATAATATTATAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAc                                       <  1:237599/148‑1 (MQ=255)
                                                                                                 acccGTGGTTTATCTCGACCCGTAAAC‑CCGTTATCAGGATCATATGAACAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATaa                                        <  2:595128/145‑1 (MQ=255)
                                                                                                 acccGGGGTTTATCTCAACCCTTTAAC‑CCGTTATCTGGTCCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                          <  2:417046/143‑1 (MQ=255)
                                                                                                  aCCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                   >  1:600202/1‑151 (MQ=255)
                                                                                                     gtggtgTATCTCAACCCGTAAAC‑CCGCTATAAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                          <  1:483529/141‑1 (MQ=255)
                                                                                                       ggtgTATCTCGAACCGTTAAC‑CCGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAg                              <  2:647158/151‑1 (MQ=255)
                                                                                                         tgtATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                            >  1:526786/1‑151 (MQ=255)
                                                                                                             tctcGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                        >  1:555853/1‑151 (MQ=255)
                                                                                                                cGACCCGTAAAC‑CCGCTATCAGGATCATATCATCAACCCCCCCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCAt                     <  1:640647/151‑1 (MQ=255)
                                                                                                                  acccttaaaa‑CCTCTATAAGAATCAAATAACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcg                           <  1:286003/136‑1 (MQ=255)
                                                                                                                          aacccGCTATCAGGATCATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAAcc              <  2:152533/146‑1 (MQ=255)
                                                                                                                          aacccGCAATCAGGATAATATGACAAACCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACaa                <  1:326425/144‑1 (MQ=255)
                                                                                                                             ccGCTATCAGGATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGa          <  2:229181/150‑1 (MQ=255)
                                                                                                                                    cAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg  >  2:175532/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                    
GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAA‑CCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCG  >  NC_000913/1376356‑1376637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑