Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,576,077	0	C	A	44.4%	44.5 / 17.1	36	D515Y (GAT→TAT)	yddB	putative TonB‑dependent outer membrane receptor
Reads supporting (aligned to +/- strand):  ref base C (15/5);  new base A (0/16);  total (15/21)
Fisher's exact test for biased strand distribution p-value = 3.22e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATG  >  NC_000913/1575929‑1576226                                                                                                                                                     |                                                                                                                                                      cgcgCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCaa                                                                                                                                                     <  1:596363/151‑1 (MQ=255)        tAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATAtt                                                                                                                                              >  2:728446/1‑151 (MQ=255)         aaCGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTc                                                                                                                                             >  1:526088/1‑151 (MQ=255)              tCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGc                                                                                                                                         <  2:287102/150‑1 (MQ=255)                 tACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCa                                                                                                                                       <  1:65988/149‑1 (MQ=255)                       ttttCTGCTGCAATCCCTTTGCCATTTCATGTTTATAAGGTTTTTTAAAATCCTGATAACGCGTCAGAGTTTTATTCCCTGATACCGTTTCCGCCCAGCTATGGCGAATATCACGTACCCCCAAATAAAAAATTTTCCCGCCATAGTaa                                                                                                                                 <  1:522768/149‑1 (MQ=255)                         ttCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGt                                                                                                                             >  2:626501/1‑151 (MQ=255)                                  aaccccATTGCCAGTTCTTCTTTATAAGGCGTTTTAAAATCCTGATACCGCGTCAGATTTTTATTCCCTGATACCGATTCCGCCCAGCTATGGCGGATATCACGTAATCCCATATAAAAAATATTCCCGCCATAGTAACGGTTATAACct                                                                                                                     <  1:162813/147‑1 (MQ=255)                                         ttGCAATTTCATCTTTATAGGGCTTTTTAAAATCTTGAAACCGCGTAAGATTTTTTTTCCCTGATCCCGTTTCCGTCCAGTTATTGCGAATATCACGTAATCCAATAAAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTa                                                                                                               <  2:718761/149‑1 (MQ=255)                                             catttCATCGTTATAGGGCGTTTTAAAATCCTGATACCGCGTCAGTTTTTTATTCCCTGATCCCGATTCCGCCCAGTTATTGCGGATATCACTTAACCCCATAAAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATc                                                                                                            <  2:498847/145‑1 (MQ=255)                                                          tagggCTTTTTCAAATCCTGATAACGCGTCAGATTTTTATTACCTAATCCCGATTCCGTCCAGCTATTGCGGATATCATATAATCCTATAACAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGAt                                                                                               <  1:250864/145‑1 (MQ=255)                                                                gTTTTCAACTCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaa                                                                                       >  1:349407/1‑150 (MQ=255)                                                                 ttttCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaaa                                                                                      >  2:55961/1‑150 (MQ=255)                                                                 ttttAAAATCTTGATAACGCGTCAGATTTTTATTACCTGATACCGATCCCGTCCAGCTATTGCGGATATCACGAAATCCCATATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCaaaaa                                                                                      <  2:205769/150‑1 (MQ=255)                                                                  tttCAAGTCCTGATAGCGCGGAGGAGTTTTTTTCCCTGATCCCGATTCCGCCCGGTTATGGGGGATGTCACGTACTCCAATATAAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAat                                                                                     <  1:28759/150‑1 (MQ=255)                                                                          ccTGATAACGCGTCAGATTTTTATTCCTTGATACCGATTCCGTCCAGCTATTGCGGATATCACTTAATCCCATATAAAAAATTTTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTc                                                                            <  2:366235/151‑1 (MQ=255)                                                                          ccTGATAACGCGTCAGATTTTTATTACCTGATACCGTTCCCGTCCAGCTATTGCGAATATCACGTAACCCCATATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTc                                                                            <  2:564980/151‑1 (MQ=255)                                                                           cTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTcc                                                                           >  1:47726/1‑151 (MQ=255)                                                                                aaCGCGTCAGATTTTTATTCCCTGATCCCGTTCCCGTCCAGCTATTGCGAATATCACGTAATCCCATATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCa                                                                       <  1:39777/150‑1 (MQ=255)                                                                                           tttttATTCCCTGATCCCGATCCCGCCCAGCTATTGCGGATATCACTTAATCCCATATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCgg                                                             <  2:85183/148‑1 (MQ=255)                                                                                           tttttATTCCCTAACCCCGATTCCGCCAAGCTATGGCGAATATCACGAAACCCAAAAAAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAg                                                                  <  1:365295/143‑1 (MQ=255)                                                                                             tttATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCgggg                                                           >  2:319255/1‑149 (MQ=255)                                                                                                  taCCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATAtt                                                    >  2:426449/1‑151 (MQ=255)                                                                                                    ccTAATCCCGTTCCCGTCCAGTTTTTGGGAATACCACGTAACCCCATAAAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGatat                                                     <  2:156494/148‑1 (MQ=255)                                                                                                            cgtttCCGTCCAGTTTTTGCGAATTTCACGTAACCCCATATAAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATtg                                                   <  1:428761/139‑1 (MQ=255)                                                                                                                 ccGCCCAGTTATTGGGAATACCCCGAAACCCAATAAAAAAAATTTCCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCACTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAg                                        <  2:538777/148‑1 (MQ=255)                                                                                                                      cAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTgt                                >  2:341932/1‑151 (MQ=255)                                                                                                                          tATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACTTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTgtcat                             >  1:70494/1‑150 (MQ=255)                                                                                                                            ttGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTgtcatagt                          >  1:398242/1‑151 (MQ=255)                                                                                                                             tGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTgtcatagtc                         >  1:135595/1‑151 (MQ=255)                                                                                                                              gCGAATATCACGTAACCCCATATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTgtcata                            <  2:447141/147‑1 (MQ=255)                                                                                                                                 gATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATAc                     >  2:151495/1‑151 (MQ=255)                                                                                                                                       aCGTACTCCAAAATAAAAAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCa                 <  1:38349/149‑1 (MQ=255)                                                                                                                                       aCGTAATCCAATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCa                 <  1:146415/149‑1 (MQ=255)                                                                                                                                              cccATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGc         >  1:477639/1‑150 (MQ=255)                                                                                                                                               ccATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCAt       >  2:626055/1‑151 (MQ=255)                                                                                                                                                     cAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATg  >  1:601911/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                      CGCGCAATAACGTTCTTACCGATTTTCTGCTGCAATCCCATTGCCAGTTCATCGTTATAAGGCGTTTTCAAATCCTGATAACGCGTCAGAGTTTTATTACCTGATACCGATTCCGTCCAGCTATTGCGGATATCACGTAATCCCATATCAAGAATATTCCCGCCATAGTAACGGTTATAACCTGCTGTAATCATTGAGGTTTGATTAGCAAAAATATCCCATTCCGTCATAAAGCGCGGGGAGATATTGTGGTTTGACAGATAGTTGTCATAGTCATACCGCACGCCGGGCATTAATG  >  NC_000913/1575929‑1576226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑