Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,927,703	G→A	intergenic (+31/‑526)	ygdH → / → sdaC	UPF0717 family protein/putative serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,927,703	0	G	A	100.0%	195.0 / NA	50	intergenic (+31/‑526)	ygdH/sdaC	UPF0717 family protein/putative serine transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/24);  total (26/24)

AACGGCGATAAAGAGATTATGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAA  >  NC_000913/2927556‑2927848                                                                                                                                                    |                                                                                                                                                  aaCGGCGATAAAGAGATTATGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGtt                                                                                                                                                <  1:658341/151‑1 (MQ=255)       gATAAAGAGATTATGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGCGACGTTTGTTGCCGAGTTTTCTCg                                                                                                                                          <  1:46820/151‑1 (MQ=255)         tAAAGAGATTATGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGtt                                                                                                                                        >  1:333330/1‑151 (MQ=255)            agagATTATGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGttttt                                                                                                                                     >  1:270942/1‑151 (MQ=255)             gagaTTATGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTg                                                                                                                                    <  2:258488/151‑1 (MQ=255)                 ttATGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTc                                                                                                                                 >  2:226488/1‑150 (MQ=255)                  tATGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCAc                                                                                                                               >  1:210457/1‑151 (MQ=255)                    tGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACtt                                                                                                                             >  1:398869/1‑151 (MQ=255)                       gtcgtATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACt                                                                                                                          <  2:510859/151‑1 (MQ=255)                            aTGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACtcatca                                                                                                                     <  1:420340/151‑1 (MQ=255)                             tGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACtcatca                                                                                                                     >  1:360859/1‑150 (MQ=255)                                  gacCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACtcat                                                                                                               >  1:301240/1‑151 (MQ=255)                                  gacCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACtcat                                                                                                               >  1:374227/1‑151 (MQ=255)                                    ccTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACtcattt                                                                                                             <  1:226438/151‑1 (MQ=255)                                         tACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTcatttcattt                                                                                                        >  1:305626/1‑151 (MQ=255)                                            aGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGt                                                                                                      >  1:21946/1‑150 (MQ=255)                                                 tttGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATg                                                                                                >  1:229657/1‑151 (MQ=255)                                                 tttGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATg                                                                                                >  1:587195/1‑151 (MQ=255)                                                       gCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGtt                                                                                          <  2:157577/151‑1 (MQ=255)                                                        cccAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGttt                                                                                         <  1:228890/151‑1 (MQ=255)                                                              aTCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTacc                                                                                   >  2:554642/1‑151 (MQ=255)                                                              aTCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTacc                                                                                   >  2:315478/1‑151 (MQ=255)                                                                cGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTaccac                                                                                 >  1:70055/1‑151 (MQ=255)                                                                 gTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTaccacc                                                                                >  2:126468/1‑151 (MQ=255)                                                                     gAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTc                                                                             >  2:73287/1‑150 (MQ=255)                                                                        gTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCCCCCTCAcg                                                                          <  1:77434/150‑1 (MQ=255)                                                                          tGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCAcgcg                                                                        >  2:17863/1‑150 (MQ=255)                                                                               ggCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAat                                                                  <  2:498025/151‑1 (MQ=255)                                                                                gCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAata                                                                 <  1:356582/151‑1 (MQ=255)                                                                                      ccTACATCCCTTGCTACGAAATCTGCGCGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATatcat                                                             <  1:439453/149‑1 (MQ=255)                                                                                          cATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGt                                                       >  2:334826/1‑151 (MQ=255)                                                                                            tCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGtt                                                      <  1:52084/150‑1 (MQ=255)                                                                                             cccTTGCTGCGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTaa                                                    <  1:243614/151‑1 (MQ=255)                                                                                              ccTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAat                                                   >  1:23696/1‑151 (MQ=255)                                                                                                 tGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAata                                                  <  1:386098/149‑1 (MQ=255)                                                                                                     aCGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCa                                             <  1:112626/150‑1 (MQ=255)                                                                                                     aCGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCAt                                            <  1:483118/151‑1 (MQ=255)                                                                                                       gAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATAc                                          >  2:616674/1‑151 (MQ=255)                                                                                                       gAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATAc                                          >  2:623917/1‑151 (MQ=255)                                                                                                         aaTCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACa                                         >  1:465467/1‑150 (MQ=255)                                                                                                              gCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAAtt                                      <  1:27581/148‑1 (MQ=255)                                                                                                               cACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCCCCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTAt                                   <  1:626944/150‑1 (MQ=255)                                                                                                                    aaTCTCCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGc                              >  2:445174/1‑150 (MQ=255)                                                                                                                        tcCGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGc                         >  1:397128/1‑151 (MQ=255)                                                                                                                         ccGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCaa                            <  1:404555/147‑1 (MQ=255)                                                                                                                         ccGCTCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCaa                            <  2:63317/147‑1 (MQ=255)                                                                                                                             tCCCCCGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACg                    >  2:15232/1‑151 (MQ=255)                                                                                                                              cccccGGTGACGTTTGTTGCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACg                    <  2:472528/150‑1 (MQ=255)                                                                                                                                              ttgCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATa    <  1:182632/150‑1 (MQ=255)                                                                                                                                                gCCGAGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATaaa  <  2:445108/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  AACGGCGATAAAGAGATTATGCGTCGTATGGACGACCTGCTACAGGGTTTTGTTGCCCAGCATCGTATGAAGTTGCCAGGCTCAGCCTACATCCCTTGCTACGAAATCTGCACGTAATCTCCGCTCCCCCGGTGACGTTTGTTGCCGGGTTTTCTCGTTTTTGGTCACTTACTCATCAACTCATTTCATTTGTTATATGAATGTTTCTTACCACCCTCACGCGACAAATATCATCACAGTTAATATGTCATACAATTTATGTTGCAACGCAAACGTTTCCCTATTTTCATAAA  >  NC_000913/2927556‑2927848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑