Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,284	C→A	G79W (GGG→TGG)	sapC ←	antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,284	0	C	A	100.0%	129.1 / NA	36	G79W (GGG→TGG)	sapC	antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/20);  total (16/20)

GAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACC  >  NC_000913/1354141‑1354429                                                                                                                                                |                                                                                                                                                  gAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGgaag                                                                                                                                            <  1:603422/151‑1 (MQ=255)   ggCCGTGTGTCGCCCCGGCGAAGGCGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGGAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGgaaga                                                                                                                                           >  1:141593/1‑150 (MQ=255)    gCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGaagaaa                                                                                                                                         <  1:718212/151‑1 (MQ=255)      cGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGgaagaa                                                                                                                                          >  2:416687/1‑148 (MQ=255)             cGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGAGCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTc                                                                                                                                 <  1:371047/150‑1 (MQ=255)                            gCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCAt                                                                                                                 <  1:325103/151‑1 (MQ=255)                            gCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCAt                                                                                                                 <  2:276767/151‑1 (MQ=255)                            gCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCAt                                                                                                                 <  1:551194/151‑1 (MQ=255)                             cccAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATg                                                                                                                >  2:620674/1‑151 (MQ=255)                                  tACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAcggc                                                                                                           >  1:389757/1‑151 (MQ=255)                                    ccAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGAcggcg                                                                                                          <  1:702449/150‑1 (MQ=255)                                        gCCACAGATCGTCGCGGCAAGCGTCTCCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaat                                                                                                     <  2:440264/151‑1 (MQ=255)                                         ccACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCaata                                                                                                    <  1:428166/151‑1 (MQ=255)                                              gATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTg                                                                                                >  2:452377/1‑150 (MQ=255)                                                      cgGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCg                                                                                         >  2:40047/1‑149 (MQ=255)                                                          aaGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAAt                                                                                   <  2:212702/151‑1 (MQ=255)                                                                caccacAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTgg                                                                             >  2:695100/1‑151 (MQ=255)                                                                   cccaAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCg                                                                          <  1:448526/149‑1 (MQ=255)                                                                           cgcCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTa                                                                  <  1:645319/151‑1 (MQ=255)                                                                              cacccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAgg                                                               >  1:104276/1‑151 (MQ=255)                                                                                cccacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGgc                                                              <  2:137973/150‑1 (MQ=255)                                                                                  cacGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGgcgc                                                            >  2:355092/1‑150 (MQ=255)                                                                                        tGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCa                                                      <  2:45849/150‑1 (MQ=255)                                                                                        tGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCa                                                      <  2:529221/150‑1 (MQ=255)                                                                                                 tCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGGAAGGCGCAAACCAGCCGCCaaa                                             >  2:158800/1‑150 (MQ=255)                                                                                                  ccGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaaaa                                            <  1:416589/150‑1 (MQ=255)                                                                                                  ccGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCaaa                                             <  2:272926/149‑1 (MQ=255)                                                                                                    gCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATa                                         <  1:642756/151‑1 (MQ=255)                                                                                                      tCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATac                                        >  2:483309/1‑150 (MQ=255)                                                                                                          tAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGt                                   >  1:393755/1‑151 (MQ=255)                                                                                                          tAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGt                                   >  2:650756/1‑151 (MQ=255)                                                                                                           aaaCGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGt                                   >  1:644661/1‑150 (MQ=255)                                                                                                              cGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTAcag                               <  1:194273/151‑1 (MQ=255)                                                                                                                         aTCGCGTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCcgcg                    <  2:545568/151‑1 (MQ=255)                                                                                                                             cgTCCGAGGTCGTCAGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGc                >  2:709962/1‑151 (MQ=255)                                                                                                                                           aGTCCACAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGAcc  >  2:372778/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                  GAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCAGTCCCCAGGAAGAAAGAAACTTCGCCATAGCGCGACCATGACGGCGGCAATAATTGATAACCGAGAAATTGCTGGTCGATGCCGTAAGGCGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACC  >  NC_000913/1354141‑1354429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑