Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,574,051 | 0 | G | T | 38.9% | 78.7 / 20.2 | 56 | V384V (GTC→GTA) | pqqL | putative periplasmic M16 family zinc metalloendopeptidase |
Reads supporting (aligned to +/- strand): ref base G (6/27); new base T (21/0); total (29/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.14e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCG > NC_000913/1573906‑1574200 | gTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGAcatc < 1:652349/151‑1 (MQ=255) gTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTAca > 1:733017/1‑149 (MQ=255) tCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTAcatca > 2:832644/1‑149 (MQ=255) cGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACatcatc < 2:1058683/150‑1 (MQ=255) gAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGa < 2:848611/151‑1 (MQ=255) gAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTAcatca > 2:385932/1‑147 (MQ=255) aaaGTTTATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAg < 2:777680/151‑1 (MQ=255) gTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GAAGATTTGACATCATCGAGttt > 1:1116928/1‑150 (MQ=255) atatGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTAAGGCGG‑GTTGAGTTAGcatccacatgttctttt > 2:190137/1‑138 (MQ=255) gTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTcag‑ca < 1:968621/150‑1 (MQ=255) gTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTcag‑ca < 1:425054/150‑1 (MQ=255) tctTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTATATCATCGAGTTTTTCAG‑CAGaa > 1:1052671/1‑150 (MQ=255) ctTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGaa < 1:771986/149‑1 (MQ=255) ttCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGTGATTTTAAAATAACGAGGTCTTcagaccaaa > 1:858875/1‑143 (MQ=255) tCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGaaaa < 2:658440/149‑1 (MQ=255) ccGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGAGATCATCCAGGTTTTTTG‑CCGAAAAAc > 1:816779/1‑150 (MQ=255) gACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCt < 1:296108/151‑1 (MQ=255) aaGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTACATCAACGAGTTTTTTCG‑CAGAAAAAACATGc > 1:1222257/1‑147 (MQ=255) aGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATc < 2:1246458/151‑1 (MQ=255) gAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGAATTTTCATCAACGAGTTTTTTAG‑CCGAAAAAACAAGCTg > 1:1041501/1‑147 (MQ=255) tattatTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTACATCATCGAGGTCTTCAG‑CAGAAAAAACATGCTGGTCAATGGtt > 1:111866/1‑149 (MQ=255) aaTGAGCTGGATGCCAGGCGACTGGTCAGCATTCGTAAATCTCGTTCAGCTTGCTCATCAATCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CTGAAAAACCTTGCTGATCAATGGTTGCCAActct < 2:226192/150‑1 (MQ=255) gAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTACATCATCGAGGTTTTCTT‑CAGAAAAACAAAACTGGTCAAAGGTTGTCAActcttc > 1:13375/1‑147 (MQ=255) aGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTTGGTTTTACATCCTCGAATTCTTCTG‑CAGAAAAAACATGCTGATCAATGGGTTCCAACTCTG‑CCa > 1:705388/1‑150 (MQ=255) aTGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATg < 1:1023938/150‑1 (MQ=255) gCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCAt < 1:1177036/151‑1 (MQ=255) cAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGAATTTATATCATCGAGGTCTTTTG‑CCGAAAAAACAAGCTGATCAAAGGGTGCCAACTCTT‑CCCTTTATGCAtt > 1:578438/1‑150 (MQ=255) aCTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCgcagca < 2:862518/151‑1 (MQ=255) tCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTTACATCATCGAGGTTTTCAG‑CAGAAAAAACATGCTGATCAATGGGTGCCACCTCTT‑CCATTAATGCATTCGCAGCATcc > 1:718022/1‑149 (MQ=255) tCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTTCATCATCGAGTTCTTTCA‑CAGAAAAAACAAGCTGATCAAAGGTTGGCAACTCTT‑CCCTTAATTCATTCgcagca > 2:787217/1‑146 (MQ=255) gCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTATATCATCGAGTTTTTTTA‑CCGAAAAAACCCGCTGATCAAAGGTTTTCAACTCTG‑CCCTTTATGTATTTGCCACATCCTGc > 2:70696/1‑149 (MQ=255) cATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAACCCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCat < 1:124592/150‑1 (MQ=255) tACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGCGTAGATTT‑TCAGCCTCGAGGTTTTTAGCCACAAAAACAAACCGGTTAAAAGGTTCCCAACCCT‑‑CCCTTTATGTATTCACAGCCACCTGCaa > 1:922800/1‑147 (MQ=255) aCGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCAtatt < 2:647802/150‑1 (MQ=255) aaaTCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCg < 2:500728/150‑1 (MQ=255) cACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTTACAACATCGAGGTCTTTAG‑CAGAAAAAACATACTGATCAAAGGTTGCCCACTCTT‑CCATTAATTCATTTGCAGCATCCTGCATATTTTTGtctc > 1:352701/1‑150 (MQ=255) cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTg < 2:1073637/151‑1 (MQ=255) cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTTTATTCTCTAGTTTTTCAG‑CCGAAAAAACCAGCTGGTTAAAGGTTGTCCAATCTGCCCTTTATTGAATTC‑CAACATCCTGCctat > 2:148593/1‑139 (MQ=255) gTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTACATAATCGAGTTTTTTTG‑CCGAAAAAACAAGCTGATCAAATGTTTTCAACCCTG‑CCCTTAATGCATTTGCAGCAACCTGCCTATTATTGt > 2:941953/1‑144 (MQ=255) aGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCAttt < 1:752535/151‑1 (MQ=255) cTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTGTAATCATCGAGTTCTTTAG‑CAGAAAAAACATGATGGTCAATAGTTGCCAACTCTG‑CCCTTAATGCATTAGCCACATCCTGCATATTATCTTCTCGTGCATTTTCACgg > 1:831071/1‑151 (MQ=255) aaCCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTTATATCACTGCGTTTTTTCG‑CAAAAAAAACATGCTGGACAAAGGGTGCCAACTCTT‑CCCTTAATGCATTCTCACGATCCTGCATATTTTTGTCCCCGGCCTTTTCACAGgaaaa > 2:512982/1‑150 (MQ=255) aCCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTCCTGCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCGTCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACa < 2:498658/151‑1 (MQ=255) cGCATTTTTCAGCCAGGTGAGGCGG‑GGAGATTTTTCATCATCGAGGTTTTTAG‑CCAAAAAAACAAGCTGGTTAATGGGTGGCAACTCCG‑CCATTAATGCATTTGCAGCCTCCCGCATATTATCGTTCCGTGCATTTAAACGGaaaaaaa > 1:13063/1‑147 (MQ=255) ttttCAGCCAGGTGAGGCGG‑GGAGATTTTACATCCTCCAGGTTTTCAG‑CCGGAAAAACATGGTGGTCAAAGGGTGGCAACTCCT‑CCCTTAATTCATTTGCAGCATCCCTCATATTATTGTCTCGGGCATTTTCAAGGAAAAAAAACGAccg > 2:220060/1‑149 (MQ=255) ttCAGCCAGGTGAGGCGG‑GTAGATTTTTCAATATCGAGTTCTTCTG‑CAGAAAAAACCCGCTGGTTAAAGGTGGCCCACACTT‑CCCTTTATTCATTTGCCACCCCCCTCATATTTTCATCTCGTGCCTTTTCAACGaaaaaaaaagaacg > 2:798027/1‑140 (MQ=255) tCAGCCAGGTGAGGCGG‑GTAGATTTGGAATCAACGGATTCTTCAT‑CAGAAAAAACATGCTTGTTAATGGGTGCCAACTCTT‑CCCTTTAAAAAATCGCAGGATCCTGCATATTATCGTCTCGTTCCATTATACCGAAAAACa > 1:985418/1‑141 (MQ=255) gCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATc < 1:1206242/151‑1 (MQ=255) ccAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACATGGAAAAACAGCGACTGATAATCg < 1:46268/151‑1 (MQ=255) gTGAGGCGG‑GTAGATTTGACATCATCGAGTGCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTg < 1:722130/151‑1 (MQ=255) gg‑gTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAAtttt < 1:349248/151‑1 (MQ=255) g‑gTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTa < 2:495498/151‑1 (MQ=255) gTAGATTTGTCATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATGATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTaa < 2:525411/151‑1 (MQ=255) gTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTaa < 2:816751/151‑1 (MQ=255) gATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAACATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGc > 2:741080/1‑151 (MQ=255) aTTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCt > 1:151963/1‑151 (MQ=255) gACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgcg < 1:385945/150‑1 (MQ=255) | GTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGG‑GTAGATTTGACATCATCGAGTTCTTCAG‑CAGAAAAACCATGCTGATCAATGGTTGCCAACTCTG‑CCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCG > NC_000913/1573906‑1574200 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |