Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,574,0510GT38.9% 78.7 / 20.2 56V384V (GTC→GTApqqLputative periplasmic M16 family zinc metalloendopeptidase
Reads supporting (aligned to +/- strand):  ref base G (6/27);  new base T (21/0);  total (29/27)
Fisher's exact test for biased strand distribution p-value = 1.14e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCG  >  NC_000913/1573906‑1574200
                                                                                                                                                  |                                                                                                                                                       
gTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGAcatc                                                                                                                                                    <  1:652349/151‑1 (MQ=255)
gTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTAca                                                                                                                                                      >  1:733017/1‑149 (MQ=255)
   tCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTAcatca                                                                                                                                                   >  2:832644/1‑149 (MQ=255)
    cGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACatcatc                                                                                                                                                 <  2:1058683/150‑1 (MQ=255)
     gAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGa                                                                                                                                               <  2:848611/151‑1 (MQ=255)
     gAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTAcatca                                                                                                                                                   >  2:385932/1‑147 (MQ=255)
      aaaGTTTATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAg                                                                                                                                              <  2:777680/151‑1 (MQ=255)
         gTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGAAGATTTGACATCATCGAGttt                                                                                                                                           >  1:1116928/1‑150 (MQ=255)
             atatGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTAAGGCGGGTTGAGTTAGcatccacatgttctttt                                                                                                                                       >  2:190137/1‑138 (MQ=255)
                 gTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTcagca                                                                                                                                   <  1:968621/150‑1 (MQ=255)
                 gTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTcagca                                                                                                                                   <  1:425054/150‑1 (MQ=255)
                    tctTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTATATCATCGAGTTTTTCAGCAGaa                                                                                                                                >  1:1052671/1‑150 (MQ=255)
                     ctTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGaa                                                                                                                                <  1:771986/149‑1 (MQ=255)
                      ttCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGTGATTTTAAAATAACGAGGTCTTcagaccaaa                                                                                                                                >  1:858875/1‑143 (MQ=255)
                       tCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGaaaa                                                                                                                              <  2:658440/149‑1 (MQ=255)
                        ccGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGAGATCATCCAGGTTTTTTGCCGAAAAAc                                                                                                                            >  1:816779/1‑150 (MQ=255)
                             gACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCt                                                                                                                      <  1:296108/151‑1 (MQ=255)
                                aaGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCAACGAGTTTTTTCGCAGAAAAAACATGc                                                                                                                       >  1:1222257/1‑147 (MQ=255)
                                 aGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATc                                                                                                                  <  2:1246458/151‑1 (MQ=255)
                                  gAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTTTCATCAACGAGTTTTTTAGCCGAAAAAACAAGCTg                                                                                                                     >  1:1041501/1‑147 (MQ=255)
                                          tattatTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGGTCTTCAGCAGAAAAAACATGCTGGTCAATGGtt                                                                                                           >  1:111866/1‑149 (MQ=255)
                                                  aaTGAGCTGGATGCCAGGCGACTGGTCAGCATTCGTAAATCTCGTTCAGCTTGCTCATCAATCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCTGAAAAACCTTGCTGATCAATGGTTGCCAActct                                                                                                  <  2:226192/150‑1 (MQ=255)
                                                     gAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATCATCGAGGTTTTCTTCAGAAAAACAAAACTGGTCAAAGGTTGTCAActcttc                                                                                                >  1:13375/1‑147 (MQ=255)
                                                      aGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTTGGTTTTACATCCTCGAATTCTTCTGCAGAAAAAACATGCTGATCAATGGGTTCCAACTCTGCCa                                                                                             >  1:705388/1‑150 (MQ=255)
                                                            aTGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATg                                                                                       <  1:1023938/150‑1 (MQ=255)
                                                              gCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCAt                                                                                    <  1:1177036/151‑1 (MQ=255)
                                                                cAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGAATTTATATCATCGAGGTCTTTTGCCGAAAAAACAAGCTGATCAAAGGGTGCCAACTCTTCCCTTTATGCAtt                                                                                   >  1:578438/1‑150 (MQ=255)
                                                                      aCTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcagca                                                                            <  2:862518/151‑1 (MQ=255)
                                                                           tCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACATCATCGAGGTTTTCAGCAGAAAAAACATGCTGATCAATGGGTGCCACCTCTTCCATTAATGCATTCGCAGCATcc                                                                         >  1:718022/1‑149 (MQ=255)
                                                                           tCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTTCATCATCGAGTTCTTTCACAGAAAAAACAAGCTGATCAAAGGTTGGCAACTCTTCCCTTAATTCATTCgcagca                                                                            >  2:787217/1‑146 (MQ=255)
                                                                              gCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTATATCATCGAGTTTTTTTACCGAAAAAACCCGCTGATCAAAGGTTTTCAACTCTGCCCTTTATGTATTTGCCACATCCTGc                                                                      >  2:70696/1‑149 (MQ=255)
                                                                               cATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAACCCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCat                                                                    <  1:124592/150‑1 (MQ=255)
                                                                                 tACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGCGTAGATTTTCAGCCTCGAGGTTTTTAGCCACAAAAACAAACCGGTTAAAAGGTTCCCAACCCTCCCTTTATGTATTCACAGCCACCTGCaa                                                                    >  1:922800/1‑147 (MQ=255)
                                                                                  aCGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCAtatt                                                                 <  2:647802/150‑1 (MQ=255)
                                                                                      aaaTCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCg                                                             <  2:500728/150‑1 (MQ=255)
                                                                                          cACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTACAACATCGAGGTCTTTAGCAGAAAAAACATACTGATCAAAGGTTGCCCACTCTTCCATTAATTCATTTGCAGCATCCTGCATATTTTTGtctc                                                         >  1:352701/1‑150 (MQ=255)
                                                                                            cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTg                                                      <  2:1073637/151‑1 (MQ=255)
                                                                                            cGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTTTATTCTCTAGTTTTTCAGCCGAAAAAACCAGCTGGTTAAAGGTTGTCCAATCTGCCCTTTATTGAATTCCAACATCCTGCctat                                                                  >  2:148593/1‑139 (MQ=255)
                                                                                             gTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTACATAATCGAGTTTTTTTGCCGAAAAAACAAGCTGATCAAATGTTTTCAACCCTGCCCTTAATGCATTTGCAGCAACCTGCCTATTATTGt                                                            >  2:941953/1‑144 (MQ=255)
                                                                                                 aGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCAttt                                                 <  1:752535/151‑1 (MQ=255)
                                                                                                       cTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTGTAATCATCGAGTTCTTTAGCAGAAAAAACATGATGGTCAATAGTTGCCAACTCTGCCCTTAATGCATTAGCCACATCCTGCATATTATCTTCTCGTGCATTTTCACgg                                           >  1:831071/1‑151 (MQ=255)
                                                                                                             aaCCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTTATATCACTGCGTTTTTTCGCAAAAAAAACATGCTGGACAAAGGGTGCCAACTCTTCCCTTAATGCATTCTCACGATCCTGCATATTTTTGTCCCCGGCCTTTTCACAGgaaaa                                      >  2:512982/1‑150 (MQ=255)
                                                                                                              aCCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTCCTGCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCGTCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACa                                    <  2:498658/151‑1 (MQ=255)
                                                                                                                cGCATTTTTCAGCCAGGTGAGGCGGGGAGATTTTTCATCATCGAGGTTTTTAGCCAAAAAAACAAGCTGGTTAATGGGTGGCAACTCCGCCATTAATGCATTTGCAGCCTCCCGCATATTATCGTTCCGTGCATTTAAACGGaaaaaaa                                    >  1:13063/1‑147 (MQ=255)
                                                                                                                     ttttCAGCCAGGTGAGGCGGGGAGATTTTACATCCTCCAGGTTTTCAGCCGGAAAAACATGGTGGTCAAAGGGTGGCAACTCCTCCCTTAATTCATTTGCAGCATCCCTCATATTATTGTCTCGGGCATTTTCAAGGAAAAAAAACGAccg                             >  2:220060/1‑149 (MQ=255)
                                                                                                                       ttCAGCCAGGTGAGGCGGGTAGATTTTTCAATATCGAGTTCTTCTGCAGAAAAAACCCGCTGGTTAAAGGTGGCCCACACTTCCCTTTATTCATTTGCCACCCCCCTCATATTTTCATCTCGTGCCTTTTCAACGaaaaaaaaagaacg                             >  2:798027/1‑140 (MQ=255)
                                                                                                                        tCAGCCAGGTGAGGCGGGTAGATTTGGAATCAACGGATTCTTCATCAGAAAAAACATGCTTGTTAATGGGTGCCAACTCTTCCCTTTAAAAAATCGCAGGATCCTGCATATTATCGTCTCGTTCCATTATACCGAAAAACa                                    >  1:985418/1‑141 (MQ=255)
                                                                                                                           gCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATc                       <  1:1206242/151‑1 (MQ=255)
                                                                                                                            ccAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACATGGAAAAACAGCGACTGATAATCg                      <  1:46268/151‑1 (MQ=255)
                                                                                                                                gTGAGGCGGGTAGATTTGACATCATCGAGTGCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTg                  <  1:722130/151‑1 (MQ=255)
                                                                                                                                       gggTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAAtttt           <  1:349248/151‑1 (MQ=255)
                                                                                                                                        ggTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTa          <  2:495498/151‑1 (MQ=255)
                                                                                                                                          gTAGATTTGTCATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATGATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTaa         <  2:525411/151‑1 (MQ=255)
                                                                                                                                          gTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTaa         <  2:816751/151‑1 (MQ=255)
                                                                                                                                             gATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAACATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGc      >  2:741080/1‑151 (MQ=255)
                                                                                                                                              aTTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCt     >  1:151963/1‑151 (MQ=255)
                                                                                                                                                  gACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTgcg  <  1:385945/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                       
GTTTCGAAAGTTGATATGTCTCTTCCGGCGACAAGAACGGCGTATTATTTAATGAGCTGGATGCCAGGCGACTGGTCAGCATACGTAAATCACGTTCAGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATTATCGTCTCGTGCATTTACACGGAAAAACAGCGACTGATAATCGGGTGCAATTTTAACGCTGCG  >  NC_000913/1573906‑1574200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: