Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,412,906	0	T	A	38.8%	138.9 / 44.7	80	Y187F (TAT→TTT)	acrS	acrAB operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (44/5);  new base A (0/31);  total (44/36)
Fisher's exact test for biased strand distribution p-value = 2.63e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAG  >  NC_000913/3412756‑3413050                                                                                                                                                       |                                                                                                                                                    aaaCTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTtat                                                                                                                                                     >  2:274292/1‑151 (MQ=255)       gTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGCGCGGGGGCTTGTTTAT‑AAAg                                                                                                                                                <  2:44693/149‑1 (MQ=255)       gTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGAt                                                                                                                                              <  1:781108/151‑1 (MQ=255)       gTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGGTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGTCCAGAGCGGGGGCTTTTTgat                                                                                                                                                     <  2:1079607/145‑1 (MQ=255)         tAACTGTGACGAAGTGAATTTTCAGGACAGACTGTGAATTTAAATGACACTTAGTTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGGGTGGGCTTGTTTAT‑AAAGAt                                                                                                                                              >  2:78988/1‑149 (MQ=255)         tAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATc                                                                                                                                             >  1:348743/1‑150 (MQ=255)         tAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATc                                                                                                                                             >  1:269610/1‑150 (MQ=255)          aaCTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑a                                                                                                                                           <  2:371619/150‑1 (MQ=255)          aaCTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑At                                                                                                                                          >  2:1080336/1‑151 (MQ=255)             tgtgACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑c                                                                                                                                      >  1:335700/1‑151 (MQ=255)              gtgACGAACTGAATTTTCAGGACAGAATGTGAATTTATATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGAACAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑cc                                                                                                                                     >  2:142386/1‑151 (MQ=255)              gtgACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTTGCATGAACATTCTTAATACGTTATCGACCCGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATaa                                                                                                                                        <  2:1138990/149‑1 (MQ=255)               tgACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑ccc                                                                                                                                    >  2:1082823/1‑151 (MQ=255)                gACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑ccc                                                                                                                                    <  2:920786/150‑1 (MQ=255)                 aCGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGc                                                                                                                                  >  1:1207639/1‑151 (MQ=255)                      cTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCatat                                                                                                                             >  2:110431/1‑151 (MQ=255)                          aTTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCAt                                                                                                                         >  2:515319/1‑151 (MQ=255)                           ttttCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCAtt                                                                                                                        >  2:914020/1‑151 (MQ=255)                               cAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAAcc                                                                                                                    >  1:349908/1‑151 (MQ=255)                                aGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAAcc                                                                                                                    >  1:797145/1‑150 (MQ=255)                                aGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAAcc                                                                                                                    >  2:1203392/1‑150 (MQ=255)                                aGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAAcc                                                                                                                    >  1:515764/1‑150 (MQ=255)                                     aaaaTGTAAATTTAAATGACTTTTAATTCATTTTTTTGATAAATTATTTTCTTTTTTTTTTCATCTGGAATAAACTTTTTAAATACGTTACCGCCAAGGGGGGGGGTTTTTTTAA‑AAAGATA‑ATAC‑CCCGCCATATTCATTAACCAGtttt                                                                                                              <  1:1115510/149‑1 (MQ=255)                                       attgtgATTTTACATGCATATAAATTCTTTTTTTTGAAAATTTTTTTTCTTTATTTTTTTATCGGGAATAAACTTTTTTAATCCATTATCAACCAGAGGGGGTTTTTTTTAAA‑AAAGACA‑AAAA‑CCCGCCATATTCATTAACCAGttt                                                                                                               <  1:465746/146‑1 (MQ=255)                                       aaTGTAAATTTACAACCTATTTATTTCTTTTGTTTGATGAATTAATTTCTTTTTTTTTACATGTGCGTAAAACATTTTTAATTCGTTACCCCCCAGAGCGGGGGTTTGTTAAA‑AAAAATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTg                                                                                                             <  1:657264/150‑1 (MQ=255)                                        aTGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGa                                                                                                            >  2:289596/1‑150 (MQ=255)                                             attttACATGACACTTAATTCATTTTTTTGATAAATTATTTTCTTTATGTTTTCATCTGGAATAACCATTCTTAATACTTTATCGACCAGGGGGGGGGTTTTTTTAA‑AAAAATC‑ATAC‑CCCGCCATATTCATTAACCAGTTTTGAACAAtt                                                                                                      <  1:1112683/149‑1 (MQ=255)                                             attttACATAACACTTAATTCATTTTTTTGATAAATTAATTTTTTTTTTTTTTCATCTGGCATAAACTTTTTTAATACTTTATCGACCAGGGGGGGGGTTTTTTTAA‑AAAGATC‑ATAC‑CCCGCCATATTCATTAACCAGTTTTg                                                                                                             <  1:739453/142‑1 (MQ=255)                                               tttaattGACATTTAATTTTTTCGTTTAATAAATTATTTTCTTTTTTTTTTCATCTGACAGAAAATTTCTAATTCCGTGACGGACCGGGGTGGGGGTTTTTTTAA‑AAAGTTA‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAAt                                                                                                       <  1:997542/142‑1 (MQ=255)                                                ttACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCg                                                                                                   >  2:945026/1‑151 (MQ=255)                                                 tACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTACATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGc                                                                                                  >  2:1215658/1‑151 (MQ=255)                                                            tAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCAcc                                                                                        >  2:849087/1‑150 (MQ=255)                                                             aattttttcTTTTGATAAATTATTTTTGTTTTTTTTTCATCTGAAATAAACTTTTTAAACCCTTCACCAACCAGAGCGGGGTTTTTTTAAA‑AAAAACC‑AAAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCAt                                                                                      <  2:573218/145‑1 (MQ=255)                                                               ttcattcGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATc                                                                                     >  2:1013140/1‑150 (MQ=255)                                                               ttcattcGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATc                                                                                     >  1:28703/1‑150 (MQ=255)                                                               ttcattcGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATc                                                                                     >  1:718696/1‑150 (MQ=255)                                                                  attcGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaat                                                                                  >  2:406773/1‑150 (MQ=255)                                                                   tttttttGATAAATTAATTTTTTAATTTTTTCATTTGGCGAAAACATTCTAAATACTTTATTTCCCAAGGCTGGGGTTTTTTTAA‑AAAAAAC‑AAAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATGCCGCTGAAGGCACCATCaata                                                                                 <  2:425470/146‑1 (MQ=255)                                                                   ttcGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCaata                                                                                 >  1:997466/1‑150 (MQ=255)                                                                         tgatgaATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAatc                                                                           >  2:1044877/1‑150 (MQ=255)                                                                              atttaattTTGTTTTTTTTTCAGCTGACAAGAACTTTCATAATACGTAACAGCCCAGGGGGGGGGTTTGTAAAA‑AAAAACA‑ACAT‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAatca                                                                          <  1:744381/144‑1 (MQ=255)                                                                              aattatttTTTTTATTTTTTCATCTGGCATAAACTTTTTAAATACTTTACCGCCCGGGGGGGGGGTTTTTTTAA‑AAAAATA‑AAAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAatca                                                                          <  1:1215721/146‑1 (MQ=255)                                                                              aattatttTCTTTTTTTTTTCATCTGGAATAAACATTCTTATTACGTTATCGCCAAGAGGGGGGGTTTTTTTAA‑AAAGATA‑AAAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATcaca                                                                     <  1:895310/151‑1 (MQ=255)                                                                               attaattTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATcaca                                                                     >  2:1208568/1‑150 (MQ=255)                                                                                ttatttttttttttttttCATCTGGCATAAACTTTTTTAATCCTTTACCGCCCAGAGGGGGGTTTTTTTTAA‑AAAGATC‑ATAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAatc                                                                           <  1:1077272/129‑1 (MQ=255)                                                                                ttatttTCTTTTTTTTTTCATGTGGCATAAACATTCTAAATACTTTACCGACCGGGGGGGGGGTTTGTTTAA‑AAAAATC‑AAAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCacaa                                                                    <  1:31970/150‑1 (MQ=255)                                                                                  aattTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACAt                                                                 >  1:8316/1‑151 (MQ=255)                                                                                    ttttttttttttttCAGCGGGGATAAACATTCTTAATACGTTATCACCCAGAGCGGGGGTTTTTTAAA‑AAAAACA‑AAAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAAtcatca                                                                       <  1:76585/133‑1 (MQ=255)                                                                                    tttgtttATTTTTTCATCGGGCATAAACATTTTTAATTCGTTATCAACCAGGGGGGGGTTTTTTTTAA‑AAAGATT‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCacaaca                                                                  <  1:272192/143‑1 (MQ=255)                                                                                    tttCGTTTTTTTTTCATCTGCAAAGAACATTCTTAATCCTTTAGCGACCAGAGGGGGGGTTTTTTAAA‑AAAGTTA‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAAtcatca                                                                       <  1:341957/143‑1 (MQ=255)                                                                                     ttCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCt                                                               >  2:149259/1‑150 (MQ=255)                                                                                         ttttttttcCATCGGACATAAACTTTTTTAATACTTTACCGCCCAGGGGGGGGCTTTTTTTAA‑AAAGATA‑AAAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAAt                                                           <  1:205643/145‑1 (MQ=255)                                                                                         ttgtgtttgcgtggggCATGAACTTTCTTAATTCTTAATCATCAAGGGGGGGGTTTTTTTAAA‑AAAAATA‑AAAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTa                                                              <  2:287752/133‑1 (MQ=255)                                                                                         ttATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATc                                                          >  2:1089995/1‑151 (MQ=255)                                                                                              tttcCATCTGGAAGGACCATTTTTAATTCTTTA‑CGGCCAGGGGGGGGGGTTTTTTTA‑AAAAATCAATAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAgg                                                      <  1:447059/150‑1 (MQ=255)                                                                                               tttCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATA‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGtt                                                    >  1:110771/1‑151 (MQ=255)                                                                                                ttCATGTGGCATAAAAATTCTAAATCCTTTATCGACCAGGGGGGGGTTTTGTTTAA‑AAAGATC‑ATAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGtt                                                    <  1:1194388/150‑1 (MQ=255)                                                                                                 tCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTAt                                                  >  1:868524/1‑151 (MQ=255)                                                                                                  cATCTGGCATGAACTTTCTAAATCCTTTACCGACAAGGGGGGGGGTTTGTTTAA‑AAAAATC‑ATAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGtt                                                    <  1:525375/148‑1 (MQ=255)                                                                                                     cTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGc                                              >  1:784470/1‑151 (MQ=255)                                                                                                     cTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGc                                              >  1:722399/1‑151 (MQ=255)                                                                                                          aTGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTacac                                         >  1:132584/1‑151 (MQ=255)                                                                                                          aTGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTaca                                          >  2:46345/1‑150 (MQ=255)                                                                                                            aaatttttttAATCCTTTATCACCCAGGGGGGGGTTTTTTTAAA‑AAAAATC‑AAAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTacac                                         <  2:335690/141‑1 (MQ=255)                                                                                                                 ttCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCtt                                   >  1:82347/1‑150 (MQ=255)                                                                                                                     tAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGc                              >  1:68560/1‑151 (MQ=255)                                                                                                                      aaTACGTTACCGACCAGAGCGGGGGTTTTTTTAA‑AAAGATC‑AAAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttg                                  <  2:1230208/146‑1 (MQ=255)                                                                                                                       aTACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTg                            >  2:1203143/1‑151 (MQ=255)                                                                                                                         aCGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGac                          >  1:450069/1‑151 (MQ=255)                                                                                                                          cttttccatccAGGGGGGGGGCTTTTTAAA‑AAACAAC‑AAAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCttgtt                                <  2:352662/135‑1 (MQ=255)                                                                                                                          cGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGac                          >  1:375535/1‑150 (MQ=255)                                                                                                                            ttATCGACAAGGGGGGGGTTTTTTTAAA‑AAAAACC‑AAAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGacac                        <  2:130396/150‑1 (MQ=255)                                                                                                                             tATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGc                      >  2:984726/1‑151 (MQ=255)                                                                                                                             tATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGc                      >  1:57902/1‑151 (MQ=255)                                                                                                                              accGACAAGGGGGGGGGTTTTTTTAA‑AAAGATC‑ATAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACg                       <  1:242689/147‑1 (MQ=255)                                                                                                                                      gggggggggTTTATTAAA‑AAAAATA‑ATAT‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATAc             <  1:708838/147‑1 (MQ=255)                                                                                                                                       agCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACt            >  2:961685/1‑151 (MQ=255)                                                                                                                                         ggggggTTTTTTTAAAAAAGATC‑AAAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACtt           <  1:945073/150‑1 (MQ=255)                                                                                                                                          ggggggTTTTTTAA‑AAAAATT‑AAAATCCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTc          <  2:314840/151‑1 (MQ=255)                                                                                                                                          gggggTTTTTTTAAAAAAAATC‑ATAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTc          <  2:269601/151‑1 (MQ=255)                                                                                                                                                ttGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCgg      >  2:108180/1‑148 (MQ=255)                                                                                                                                                   tttAA‑AAAAATA‑ATAC‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGagag  <  1:1044927/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                    AAACTGGTTAACTGTGACGAACTGAATTTTCAGGACAGAATGTGAATTTACATGACACTTAATTCATTCGTTTGATGAATTAATTTCGTTATGTTTTCATCTGGCATGAACATTCTTAATACGTTATCGACCAGAGCGGGGGCTTGTTTAT‑AAAGATC‑ATAA‑CCCGCCATATTCATTAACCAGTTTTGAACAATTCCGCTGAAGGCACCATCAATAATAATCATCACAACATCTAAATCGAGGTTATTTGCTACACAACCTTGTTGCTGACACGCCTGCAATACTTCGCGGAGAG  >  NC_000913/3412756‑3413050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑