Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,443,277	0	A	C	40.0%	109.4 / 26.0	71	V274G (GTA→GGA)	secY	preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (38/4);  new base C (0/28);  total (38/33)
Fisher's exact test for biased strand distribution p-value = 1.29e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443129‑3443427                                                                                                                                                       |                                                                                                                                                       aGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTAcc                                                                                                                                                      >  1:1209748/1‑151 (MQ=255)  gACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGTTGGTCGCCGGGAACAGAATAAAACTGGAACCAAAAATTGCCGG‑GATTAccc                                                                                                                                                     >  2:95838/1‑151 (MQ=255)  gACGCATAAAGTAACACATAAAGCGGTTGCCCAGTCTGCAAATACAGCAAAATTG‑TTGTCAGCCAGGTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTAccc                                                                                                                                                     <  1:738336/151‑1 (MQ=255)   aCGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTAcccc                                                                                                                                                    >  2:995281/1‑151 (MQ=255)      cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑CTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGc                                                                                                                                                 <  2:973359/151‑1 (MQ=255)         agagTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCat                                                                                                                                              >  2:610430/1‑151 (MQ=255)               acacATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACTCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCAc                                                                                                                                        >  1:1129630/1‑151 (MQ=255)                   aaaaaGCGTTTGCCAAGGCTAAAAATACAGAAAATTTG‑TTGCCAGCCGTTCCCAACCAGTCCCCCCCCCAAACCATGACGCGATGGTCGCGGGGAACAGAATAATACTGGAAGCGAAGTTTGCGGG‑GATTCCCCCCGCCATATTCACttt                                                                                                                                     <  1:617907/148‑1 (MQ=255)                   aTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTc                                                                                                                                    >  2:1157577/1‑151 (MQ=255)                    tAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTc                                                                                                                                    >  2:314219/1‑150 (MQ=255)                     aagggggTGGCCCAGGCAGAAAAAACAGAAAAATTG‑TTGCCAGCCAGTTCAAACCATTCCCGCCCCCGACCAATGCGGGGAGGGCCCCGGGAAAAAAAAAAATACTGAAAGAGAAGTTTGCCGG‑TATCCCCCCCGCCATATTCACTTTCAg                                                                                                                                  <  2:121778/146‑1 (MQ=255)                      aaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGc                                                                                                                                 >  1:467593/1‑151 (MQ=255)                          gtttgccaggccggaaaaTACAG‑GAAATTGTTTGCCAGCAAGTCCAACCCAGCCCCCCCCCCGACCAAGGACGGGATGTTCGCGGGAAAAAAAATAATACTGGAAGAGAAGATTGCCGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                             <  1:472954/136‑1 (MQ=255)                            ttccccAGGCTGAAAATACAGAAAATTTT‑TTGCCACCCATTCCCAACCAGTCCCCCCCCCAAACCATGACGCGATGGTCGCGGGAAAAAAAATAATACGGGAAGCGAAATTTGCCGG‑GTTTCCCCCCGCCATATTCACTTTCAGCGGTa                                                                                                                             <  1:271845/146‑1 (MQ=255)                                     cTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCACCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctc                                                                                                                  >  2:463281/1‑151 (MQ=255)                                      tGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGctct                                                                                                                 >  2:1136591/1‑151 (MQ=255)                                        cAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                               >  2:1215017/1‑151 (MQ=255)                                               aGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  2:513949/1‑151 (MQ=255)                                               aGCAAATTTG‑TTGCCAGCCAGTTCCACCCAGTACCCCCCCCGACCAATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑ATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        <  2:51819/151‑1 (MQ=255)                                                gCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                        >  2:10432/1‑150 (MQ=255)                                                     aTTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                                  >  1:404893/1‑151 (MQ=255)                                                      ttg‑ttgTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                                  >  1:364891/1‑150 (MQ=255)                                                      ttg‑ttgTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                                 >  1:778992/1‑151 (MQ=255)                                                      ttg‑ttgCAAGCCCGTCCAAACCACCCCCCCCCCCAACCCAGGACGCGATGGTGGCCGGGAACAAAATAATGCTGAAAGCGAAGAGGGCCGG‑TTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGaca                                                                                                   <  2:1152940/149‑1 (MQ=255)                                                        g‑ttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacga                                                                                                >  1:527588/1‑150 (MQ=255)                                                             tCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGAcc                                                                                           >  1:58347/1‑151 (MQ=255)                                                                 ccattcccACCCAGTCCCCCCCCCGACCCATGCCGGGAGGGTCGCGGGAAACAAAAAAATACTGGAAGCGAAGATTGCCGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                                                                        <  1:230429/144‑1 (MQ=255)                                                                     ttCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                                   >  2:654701/1‑151 (MQ=255)                                                                       ccACCCAGCCCCCCCCCCAACCCATGACGCGATGGTCGCCGGAAACAAAAAAATACTGAAAGCAAAGATTGCGGG‑GATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                                  <  1:616466/150‑1 (MQ=255)                                                                       ccAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                                 >  2:974638/1‑151 (MQ=255)                                                                         acccagacccccccccAAACCATGCCGGGATGGTCGCCGGAAACAGAATAATACTGAAAGCAAAGTTTGCCGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACgacgac                                                                                            <  1:1160478/127‑1 (MQ=255)                                                                         acccAGCACCCCCCCCGCCCCATGGCGGGGTGGTCGCGGGAAACAAAAAAATACTGGAAGCGAAGATCGCGGG‑TTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGAc                                                                                   <  2:697650/145‑1 (MQ=255)                                                                         aaCCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGttt                                                                               >  1:957619/1‑151 (MQ=255)                                                                           ccagccccccccccGAACCATGCCGGGATGGTCGCCGGAAACAGAATAATCCTGAAAGCGAAGATTGCGGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                                  <  1:718224/137‑1 (MQ=255)                                                                           ccAGTCCCCCCCCCGAACCATGACGCGATGGCCGCCGGGAACAAAAAAATACGGGAAGCAAAGATTGCCGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGtt                                                                                <  1:621058/148‑1 (MQ=255)                                                                            cAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTc                                                                              >  2:985931/1‑149 (MQ=255)                                                                               tACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                          >  2:527160/1‑150 (MQ=255)                                                                                   gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                     >  1:1070835/1‑151 (MQ=255)                                                                                   gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                     >  1:71358/1‑151 (MQ=255)                                                                                       ccGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAcc                                                                  >  2:710631/1‑150 (MQ=255)                                                                                            ccATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg                                                            >  2:1208745/1‑151 (MQ=255)                                                                                             caggaggggagggTCGCGGGAAACAAAAAAATACTGGAAGCAAATATGGCGGG‑TATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                             <  2:30481/138‑1 (MQ=255)                                                                                                gACGGGATGGTGGCGGGGAAAAGAAAAATACTGGAAGCCAA‑TGTGCCGGAGATTACCACCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgcggc                                                        <  1:123968/151‑1 (MQ=255)                                                                                                   gcgATGGTCGCGGGAAACAAAATAATGCTGGAAGAAAAGATGGCGGG‑GATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                      <  2:1180370/150‑1 (MQ=255)                                                                                                    tgatggccgcgaaaaaCAGAAAAATATTGGAAGCAAAGTTGGCGGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGt                                                      <  2:573223/135‑1 (MQ=255)                                                                                                     gATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTg                                                    >  1:541353/1‑150 (MQ=255)                                                                                                        ggccgcgggaaaaaaaataatACTGGAGGGGAAGTTTGCCGG‑GTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCa                                                <  1:738853/136‑1 (MQ=255)                                                                                                         gccGCCGGAAAAAAAATAATACTGAAAGCGAAGATGGCCGG‑GTTCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                               <  1:95841/149‑1 (MQ=255)                                                                                                          tCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACg                                              >  1:405506/1‑151 (MQ=255)                                                                                                            gCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCt                                            >  1:1256708/1‑151 (MQ=255)                                                                                                               gggAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                          >  1:822258/1‑150 (MQ=255)                                                                                                                    aaaaataatATGGGAAGAGAAGATTGCGGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                    <  2:413933/148‑1 (MQ=255)                                                                                                                          aataCTGGAGGCAAAGTTTGCCGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaac                               <  1:1215080/150‑1 (MQ=255)                                                                                                                                gaaagaaaaGATTGCGGG‑AATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                         <  2:1004312/143‑1 (MQ=255)                                                                                                                                  aaccGAAGATTGCCGG‑AATTTCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAg                           <  1:867867/143‑1 (MQ=255)                                                                                                                                   aGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                     >  1:320900/1‑151 (MQ=255)                                                                                                                                   aGCGAAGATCGCCGT‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                     <  1:33082/151‑1 (MQ=255)                                                                                                                                      aaaatttGCCGG‑GATACCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTc                     <  1:691849/143‑1 (MQ=255)                                                                                                                                       atgATGGCGGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                  <  2:320891/148‑1 (MQ=255)                                                                                                                                       aaGTTTGCCGG‑TTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt                  <  1:43159/150‑1 (MQ=255)                                                                                                                                       aaGTTTGCCGG‑GATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGaa                         <  1:324333/143‑1 (MQ=255)                                                                                                                                       aaGATTGCGGG‑GATCCCCCCCGCCAGATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                      <  1:722930/146‑1 (MQ=255)                                                                                                                                             gCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat            >  2:807767/1‑150 (MQ=255)                                                                                                                                             gCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat            >  2:1132211/1‑150 (MQ=255)                                                                                                                                               cGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact         >  2:967385/1‑151 (MQ=255)                                                                                                                                                gg‑gATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact         >  2:792188/1‑150 (MQ=255)                                                                                                                                                gg‑gATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatacta        <  2:1079709/151‑1 (MQ=255)                                                                                                                                                 ggaaTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatact         <  1:916557/147‑1 (MQ=255)                                                                                                                                                 ggaaTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaat            <  1:696249/144‑1 (MQ=255)                                                                                                                                                 g‑gATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatacta        >  1:1027992/1‑150 (MQ=255)                                                                                                                                                     ttACCCACGCCATATTCACTTTCAGCGGTAAATGAGTACTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACATAGAACGTCACTGCAaatactaat      >  2:101535/1‑149 (MQ=255)                                                                                                                                                       aCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTg  >  1:506459/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                       AGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTG‑TTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGG‑GATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAATACTG  >  NC_000913/3443129‑3443427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑