Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,174,605	0	T	C	41.0%	112.1 / 30.7	78	G187G (GGA→GGG)	ycfT	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (36/10);  new base C (0/32);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.90e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGAT  >  NC_000913/1174457‑1174754                                                                                                                                                      |                                                                                                                                                      acaacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTcc                                                                                                                                                     <  2:328921/151‑1 (MQ=255) acaacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTcc                                                                                                                                                     >  1:546807/1‑151 (MQ=255) acaacCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTcc                                                                                                                                                     >  2:1194540/1‑151 (MQ=255)     ccAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCccac                                                                                                                                                 <  2:1260595/151‑1 (MQ=255)      cAGGCACCGACCGCCTGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCccacc                                                                                                                                                <  2:592885/151‑1 (MQ=255)       aGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCccacc                                                                                                                                                >  2:455776/1‑150 (MQ=255)         gCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACg                                                                                                                                             <  1:1407699/151‑1 (MQ=255)           accgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGc                                                                                                                                           >  2:1404440/1‑151 (MQ=255)             cgaccgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGt                                                                                                                                         <  1:221608/151‑1 (MQ=255)                 cgCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTggg                                                                                                                                      >  1:1032122/1‑150 (MQ=255)                   ccAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAAc                                                                                                                                   >  2:457809/1‑151 (MQ=255)                   ccAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAAc                                                                                                                                   >  1:1422603/1‑151 (MQ=255)                    cAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACg                                                                                                                                  >  2:593012/1‑151 (MQ=255)                     aGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGa                                                                                                                                 <  2:279033/151‑1 (MQ=255)                       aaCGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGaa                                                                                                                                >  1:974537/1‑150 (MQ=255)                          ggTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTa                                                                                                                            <  1:50906/151‑1 (MQ=255)                                      agCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTc                                                                                                                >  2:584230/1‑151 (MQ=255)                                         cATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTc                                                                                                                >  2:975449/1‑148 (MQ=255)                                                aatgggcggcGAACCGCCCCTTTTTTAACACAGGACTTTTTTGTTGCCCAAACCCAGGCGCAAGGGTTGAAAAAAGGAAAATGGC‑GGACCCCACTTTTAATCCCCCCCCCGGGCGGGGAAAAAAAATTAACCGCCACACTcagca                                                                                                            <  2:1365966/141‑1 (MQ=255)                                                 gATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTacaa                                                                                                      >  2:715384/1‑150 (MQ=255)                                                   tGGGGGCGCAACGGCCCCTCTTTAACACAGGTAATTATTGTTGCGCAAAACCAGGCGCAAGGGCTGTAATAAGGAAAATGGC‑GAATCACCCTTTTCATCCCCCCCCACGGCGGGGGAACAAAATAAACCGCCACACTCAGCAGTaca                                                                                                       <  2:1201074/147‑1 (MQ=255)                                                    ggcggcGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTacaaaca                                                                                                   >  1:373627/1‑150 (MQ=255)                                                     gcggcgCAACGGCACCTTTTTAACACAGGTCTTTATTGTTGCGCCAAACCAGGCGCCAAGGCTGTAATAAGGCAAATGGC‑GGATCACACTGTTCATTCCCCCCCACGGCGTGGGAACAAAATTAACCGCCACACTCAGCAGTacaaaca                                                                                                   <  1:1065869/149‑1 (MQ=255)                                                       ggcgCAACGGCCCCTCTTAAACACAGGTCATTATTTTTGCGCAAACCCATGCCCCAAGGCTGTAATAAGGCAATTTGG‑GGATCACTCTTTTCATCCCCCCCCCCGGCGGGGAAACAAAATAAACCGCCACACTCAGCAGTACAAACAAgg                                                                                                <  1:1434764/150‑1 (MQ=255)                                                         cgcAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGAAGTACAAACAAGGCg                                                                                              >  2:1280871/1‑150 (MQ=255)                                                         cgaaaCGCCCCCTCTTAAACACAGGTCATTTTTGTTCCCCAAACCCATGCCCCAGGGTTGAAATAAGGCAATTTGC‑GAATCACCCTGTTCTTCCCCCCCCCCGGCGTGGGAACAAAATTAACCGCCACACTCAGCAGt                                                                                                          <  2:621473/135‑1 (MQ=255)                                                          gcAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGa                                                                                             >  1:1363916/1‑150 (MQ=255)                                                            aaCGCCCCCTCTTTAACACAGGTTATTATTGTTGCGCAAACCCATGCGCCAAGGCTGTAAAAAGGCAAATTGC‑GGATCCCACTGTTCATCCCCCCCCCCGGGGTGGGAAAAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGaa                                                                                            <  1:282737/149‑1 (MQ=255)                                                              cgcccccTCTTTAACACAGGTCATTATTGTTGCGCAAACCCAGGCGCAAAGGCTGAAATAAGGCAAATTGC‑GGAACCCACTTTTCTTCCCCCCCCGGGGGGGGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCg                                                                                              <  2:102657/140‑1 (MQ=255)                                                                  aCCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGc                                                                                     >  1:177911/1‑150 (MQ=255)                                                                    ctctTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAgc                                                                                  >  1:672343/1‑151 (MQ=255)                                                                    ctctTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTAAAAACAAGGCGAATAGTGGCAg                                                                                   >  2:990904/1‑150 (MQ=255)                                                                       tttAACAAAGTTCATTTTTGTTGCGCAAACCCATGCCCAAAGGCTGTAAAAAGGAAAATTGC‑GGACCACACTGTTCATCCCCCCCCGGGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAgc                                                                                  <  2:1298364/148‑1 (MQ=255)                                                                          aaCACAGGTAATTTTTGTTCCACCAACCCATCCGCCAGGGCTGAAAAAAGAAAATTTGC‑GGACCCCCCTTTTCTTCCCCCACCAGGGGGGGGAAACAAAATTACCCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAg                                                                             <  2:496996/150‑1 (MQ=255)                                                                             acaGGTATTTTTTGTTGCGCAAAACCATGCGCAAGGGCTAAAAAAAGGAAAATTGC‑GGATCACCCTTTTCATCCCCCCCCCCGGCGTGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACg                                                                          <  1:169605/150‑1 (MQ=255)                                                                                ggTAATTATTGTGGCCCAAACCCATGCGCAAAGGCTGTAAAAAGGAAAATTGC‑GGATCACACTTTTCTTCCCCCACCCCGGCGTGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCt                                                                       <  1:717159/150‑1 (MQ=255)                                                                                 gTCATTATTGTTGCGCAAACCCGTGCGCAAAGGTTGTAAAAAGGAAATTTGC‑GGATCACACTGTTCTTCCCCCACCACGGCGGGGAAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACgg                                                                         <  2:577413/147‑1 (MQ=255)                                                                                  tCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaa                                                                     >  2:353611/1‑150 (MQ=255)                                                                                   cttttttttggcgcCAAACATGGCGCCAAGGTTTTAATAAGCAAATTTGC‑GCACCTCACTGTTCATCCCCCCCCACGCCGGGGGAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCa                                                                              <  1:127130/130‑1 (MQ=255)                                                                                     ttttggtggcgcCACACCCGGCCCCAAGGCTTTAA‑AAACCAATTTGCTGAACCCCATTTTTCCTCCCCCACCGCGGCGAGGAAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaaa                                                                   <  2:262720/141‑1 (MQ=255)                                                                                     ttattTTCGCGCCAACCCTTGCGCCAAGCCGAAAATAAGGCAAATTGC‑GGACCCCACTTTTCTTCCCCCCCCGCGGGGGGGGAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaaaa                                                                  <  2:669378/150‑1 (MQ=255)                                                                                      tattGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAAtt                                                                >  1:1078120/1‑151 (MQ=255)                                                                                      tattGTTGCGCCAAACCATGCGCAAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCTCACCACGGCGCGGGCACACAATTAACCGCCACACTCTGCAGTACAAACAAGTCGAATAGTGGCAGCGCCAGGCGGCTaaaaaat                                                                >  2:1392115/1‑149 (MQ=255)                                                                                      tattGTTGCGCCAAACCAGGCGCCAAGGCTGTAAAAAGGAAAATGGG‑GGATCACACTGTTCTTCCCCCCCCCCGGCGGGGGAAAAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaaaa                                                                  <  2:1247811/149‑1 (MQ=255)                                                                                         tgttgCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTTTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAAtttt                                                              >  1:263243/1‑150 (MQ=255)                                                                                             gcgcAAAACAAGGCGCCAAGGTTGTAAAAAGGAAATTTGC‑GGACCCCACTTTTCATCCCCCACCCCGGGGTGGAAAAAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACAt                                                          <  1:899215/150‑1 (MQ=255)                                                                                              cgcCAAACCATGCGCAAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCTTCCCCCACCAGGGGGTGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACAt                                                          <  1:1019123/149‑1 (MQ=255)                                                                                               tcttccgatccccccAAGGCTGTAATAAGGCAAATGGC‑GGATCACCCTTTTCATCCCCCCCCCCGGGGTGGAAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATa                                                         <  1:1460942/136‑1 (MQ=255)                                                                                                ccAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATAcc                                                       >  1:1383508/1‑150 (MQ=255)                                                                                                ccAAACCATGCGCAAGGGCTGTAAAAAGGAAAATTGC‑GGATCACACTTTTCTTCCCCCCCCCCGGCGTGGGAACAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATAc                                                        <  2:698366/149‑1 (MQ=255)                                                                                                  acccctgacgcAAAGGGTGAAAAAAGCAAAGTGGC‑GGACCACACTTTCCTTCCCCCACCGCGGTGTGGAAACAAAAATAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACg                                                    <  2:70550/143‑1 (MQ=255)                                                                                                  aaaCAATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACCCTTTTCTTCCCCCCCCACGGCGTGGAAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCAc                                                     <  1:559586/150‑1 (MQ=255)                                                                                                    aCCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGCGGCAGCGACAGACGGCTAAAAATTTTACCTACCACg                                                    >  2:297025/1‑149 (MQ=255)                                                                                                     ccaggccacAAGGCTGTAAAAAGCAATATTGA‑GCTTCACCATTTTCTTCCCCCACCGCGGCGGGGGAAAAAAATAATCCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGaaa                                                 <  2:624516/143‑1 (MQ=255)                                                                                                     ccATGCGCCAAGGCTGTAAAAAGGAAAATTGC‑GGATCACACTGTTCATCCCCCCCCACGGCGTGGAAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGaaa                                                 <  1:90703/151‑1 (MQ=255)                                                                                                        ggcgcCAGGGCTGTAAAAAGCAAAATTGC‑GGATCCCCCTTTTCATTCCCCCCCGGGGGGGGGAAACAAAATTACCCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATa                                               <  1:1180090/149‑1 (MQ=255)                                                                                                          cgcCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATa                                                         >  2:1460863/1‑138 (MQ=255)                                                                                                            ccAGGGCTTTAATAAGGCAAATTGC‑GGATCACACTTTTCATCCCCCCCCACGGCGTGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACa                                           <  1:332187/150‑1 (MQ=255)                                                                                                            ccAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGCGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACa                                           >  2:354541/1‑150 (MQ=255)                                                                                                              agggCTGAAAAAAGGCAAATTGC‑GGATCCCTCTTTTCTTCCCCCACCGGGGCGGGGAAAAAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAAtt                                        <  2:389706/149‑1 (MQ=255)                                                                                                              aaGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAAtt                                        >  1:1470991/1‑151 (MQ=255)                                                                                                                ggttgaaaaaaGGAAAATGGC‑GGTCCCCCCTTTTCTTCCCCCCCCCCGGGGTGGAAAAAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTaa                                      <  2:194091/142‑1 (MQ=255)                                                                                                                    gTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGc                                   >  2:616136/1‑150 (MQ=255)                                                                                                                     taataaGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCAt                                 >  2:1374727/1‑151 (MQ=255)                                                                                                                      aaaagggaaaaTGGC‑GAATCACCCTGTTCATCCCCCCCCCCGGCGTGGGAACGAAATGAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCa                                  <  1:1003269/141‑1 (MQ=255)                                                                                                                       ataaGTCAAATTGG‑GAATCACTCTGTTCATCCCCCACCACGGCGGGGAAACGAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATAc                               <  2:1350879/151‑1 (MQ=255)                                                                                                                          aGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATAc                               >  1:1441292/1‑148 (MQ=255)                                                                                                                              aaaTTGC‑GGATCACACTGTTCATTCCCCACCACGGCGGGGAAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGAt                           <  1:128598/148‑1 (MQ=255)                                                                                                                               aattgggggATCACACTGTTCATCCCCCCCCCCGGGGGGGAAAAAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATAcc                        <  1:537668/144‑1 (MQ=255)                                                                                                                                aTTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACca                       >  2:321784/1‑150 (MQ=255)                                                                                                                                  tGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACcaca                     >  2:425217/1‑150 (MQ=255)                                                                                                                                   gC‑GGATCACCCTTTTCTTCCCCCCCCGGGGGGGGGAAAAAAAATAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACcaca                     <  2:858533/149‑1 (MQ=255)                                                                                                                                      ggATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGc                  >  1:1464913/1‑150 (MQ=255)                                                                                                                                      ggATCACACTGTTCATCCCCCCCCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACcaca                     <  1:226824/147‑1 (MQ=255)                                                                                                                                        aTCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTgg               >  1:230487/1‑151 (MQ=255)                                                                                                                                        aTCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTgg               >  1:953381/1‑151 (MQ=255)                                                                                                                                               ttttCATCCCCCCCCCGGGGGGGGGAACAAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTg          <  1:965721/147‑1 (MQ=255)                                                                                                                                               tGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAagctggagctgg         >  2:1267964/1‑150 (MQ=255)                                                                                                                                                      tCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGat  >  1:1060620/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                      ACAACCAGGCACCGACCGCCAGAACGGTAAGCAAAGAAGCCATCAGCAGATGGCGGCGCAACGGCACCTCTTTAACACAGGTCATTATTGTTGCGCCAAACCATGCGCCAAGGCTGTAATAAGGCAAATTGC‑GGATCACACTGTTCATTCCCCACCACGGCGTGGGAACGAAATTAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGAT  >  NC_000913/1174457‑1174754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑