Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,376,505	0	A	C	45.2%	119.7 / 22.7	73	T158P (ACC→CCC)	ycjR	putative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (37/3);  new base C (0/33);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.13e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGG  >  NC_000913/1376357‑1376655                                                                                                                                                            |                                                                                                                                                        cTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACAcc                                                                                                                                                       >  2:165291/1‑151 (MQ=255) cTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACAcc                                                                                                                                                       >  2:772395/1‑151 (MQ=255)    aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACAcc                                                                                                                                                       <  2:454858/148‑1 (MQ=255)       gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGcc                                                                                                                                                 >  2:347472/1‑151 (MQ=255)          gATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGAt                                                                                                                                              >  1:236051/1‑151 (MQ=255)             gACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGc‑c                                                                                                                                          <  2:335189/151‑1 (MQ=255)                cTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑ccgc                                                                                                                                       >  1:1357904/1‑151 (MQ=255)                 tCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑ccgcc                                                                                                                                      >  2:864617/1‑151 (MQ=255)                 tCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑ccgc                                                                                                                                       >  2:606999/1‑150 (MQ=255)                 tCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑ccg                                                                                                                                        <  1:1029597/149‑1 (MQ=255)                             cTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACAtcgtcg                                                                                                                          >  2:1121755/1‑151 (MQ=255)                               ggACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGa                                                                                                                         >  1:1119075/1‑150 (MQ=255)                                      gACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATc                                                                                                                 >  1:497224/1‑151 (MQ=255)                                      gACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATc                                                                                                                 >  1:1159661/1‑151 (MQ=255)                                        cgaaaaaaGGGTGGGTTTTCCCCGGCGCGTCCGGGAAGGGGCCCCCCCGCGTCCGGGAGCG‑GT‑GG‑TTTACCTCGAC‑CCGTAAA‑C‑CCGT‑TATAAGAATAATATAACCAACCCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCt                                                                                                                <  2:805502/146‑1 (MQ=255)                                        ccGCAAAAGGGTGAGTGATCCCCGGCGCGTCTTGAAACAGGGCGCCGCGCGTCCCGGACCC‑GT‑GG‑TTTTCCTCCAC‑CTGTAAA‑A‑CCCC‑AATAAGAAACATTTGAAAAACCCCCCCGCGGTTGC‑CCGCCGTTACATCGTCGAAAACGATc                                                                                                                 <  1:391727/149‑1 (MQ=255)                                           cAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                                             >  1:628542/1‑150 (MQ=255)                                            aaagTGGTGGGTGTTCCCCGGCGCGTGCTGGAACGGGCCGCCGCGGTTCCCGGCCCT‑GT‑GG‑TTTTCCCCGAC‑CCTTAAA‑C‑CCCC‑TATCAGGATAATATAACCACCCCCCCCGCGGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                           <  2:439673/151‑1 (MQ=255)                                              aaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACAt                                                                                                         >  2:354293/1‑151 (MQ=255)                                                tGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGt                                                                                                       >  1:1327193/1‑151 (MQ=255)                                                 ggTGAGTGATTCCCGGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGACCG‑GT‑GG‑TGTATCCCGAC‑CCTTAAA‑A‑CCGC‑TATCAGAATCATATGACCACCCCCCCCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTa                                                                                                      <  1:1086328/151‑1 (MQ=255)                                                      gtgtTTCCCTGCGCGTACTGGAACGGGCCGCCGCGCGTACCGGAACG‑GT‑GG‑TGTATCTCAAA‑CCTTAAA‑C‑CCGC‑TATCAGGATCATATGAACAACCCCCCCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAg                                                                                                   <  1:1221041/149‑1 (MQ=255)                                                       tgcttCCCGGCGCTTACTGGAACAGGCCCCCGCGCGTACCGGACCC‑GT‑GG‑TTTATCTC‑AA‑CCCTTAA‑ACCCGC‑AATCAGAATCATATAATCAACCCCCCCGCCGAGGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAg                                                                                                   <  2:545963/145‑1 (MQ=255)                                                        gaTCCCCTGCGCGTACGGGACCAGGTCGCGGCGGGCCCGGGACCG‑GT‑GG‑TGTTCCTCGAG‑CCTTTAA‑C‑CCGC‑TTTCAGATTCATGTGCGCACCCCCCCCCCCG‑TGCGCCGCCGTTACATCGTCGAAAACGATCTTaaa                                                                                                            <  2:1230720/138‑1 (MQ=255)                                                            cccTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg                                                                                           >  1:1306217/1‑151 (MQ=255)                                                             ccTGCGCATATGGGAACGGCTCCCGGCCCGTCCCGGACCG‑GT‑GG‑TTTTTC‑CGAA‑CCTTTAACC‑CCGC‑TAGCGGTATCATAAGACCAACCCCCCCCCCTGGGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCg                                                                                            <  2:811336/149‑1 (MQ=255)                                                               tGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCg                                                                                         >  2:1327377/1‑150 (MQ=255)                                                                     tACTGAAACGGCTCGCCGCGGGTCCCGAACCG‑GG‑GG‑TTTATCAAG‑A‑CCATTAA‑ACCCGC‑AACCAGAAAAATATGACAAACCCCCTCGCCGTTGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCt                                                                                   <  1:7363/150‑1 (MQ=255)                                                                      acggaaagggggccccgcCCGTCCGGGACCG‑GT‑GG‑TTTATCCCAAA‑CCTTAAA‑C‑CCGC‑TATGAGAATAATATGATCAACCCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTAt                                                                                 <  2:647124/137‑1 (MQ=255)                                                                           accagccccccgcGCGTCCCGGAACC‑GT‑GT‑TTTATCTCGAC‑CCTTTAA‑C‑CCGT‑TATGGGAATCATATGATCACCCCCCCCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATc                                                                                <  2:236049/138‑1 (MQ=255)                                                                              ggttCGCCGCGCGT‑CCGAAAAC‑GTGGG‑TGTCTCTCAAA‑CCGTTAA‑C‑CCGC‑TATCAGAATAATATAACCAACCCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                           <  2:167299/146‑1 (MQ=255)                                                                              gggTCGCCGCGCGTCCCGGACCC‑GT‑GG‑TGTATCTCGAA‑CCTTAAA‑C‑CCGC‑TATCAGGATAATATGATCACCCCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGa                                                                          <  1:282645/149‑1 (MQ=255)                                                                               ggTCGCCGCGCGTCCCGGACCG‑GT‑GT‑TTTTTCCAGAC‑GCTTAAA‑C‑CCGT‑AAAAAGGATAATATAACAAACCCCCCCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGaa                                                                         <  1:641775/150‑1 (MQ=255)                                                                               ggTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAat                                                                        >  1:1087891/1‑151 (MQ=255)                                                                                 tCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata                                                                       >  2:898577/1‑150 (MQ=255)                                                                                  cgccgcGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATc                                                                     >  1:155926/1‑151 (MQ=255)                                                                                  ccccgcGCGTCCCGGACCC‑GT‑GG‑TGTATCCCGAA‑CCTTAAA‑C‑CCTT‑TATCGGAAAAAAATGACAAACCCCCCCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATc                                                                     <  2:139206/149‑1 (MQ=255)                                                                                    ccgcGCGT‑CCCGAACC‑GG‑GGTTGTATCTCGAG‑CCTTAAA‑A‑CGAC‑TACCAGAATCATATGACAACCCCCCGCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                                    <  1:772411/150‑1 (MQ=255)                                                                                          ttccgggACCC‑GG‑GG‑TGTACCGCGAA‑CC‑TTAA‑A‑CCCCTTATAAGGATAATTTGAACACCCCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaa                                                                  <  1:544817/141‑1 (MQ=255)                                                                                          gtcccGGACCT‑GT‑GG‑TTTACCTCGAA‑CCTTAAA‑C‑CCCC‑TATCAGGATAATATGACCAACCCCCCCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGaagaag                                                              <  2:413512/147‑1 (MQ=255)                                                                                             cccgAACCGGT‑GG‑TGTACCTCGAC‑CCTTAAA‑C‑CCGC‑TATCAGTAACATATTAACACCCCCCCCGCCGAGGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAagaaga                                                             <  1:884024/146‑1 (MQ=255)                                                                                              cGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAc                                                         >  2:416600/1‑151 (MQ=255)                                                                                                gAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCt                                                       >  1:321251/1‑151 (MQ=255)                                                                                                 ggaccggggg‑tgTCTCTCAAC‑CCTTTAA‑C‑CCGC‑TATCAGGACAATATGATCAACCCCCCCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAAc                                                         <  1:1451132/142‑1 (MQ=255)                                                                                                 aaCC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                      >  1:823803/1‑151 (MQ=255)                                                                                                  accggt‑gg‑tg‑ATCCCGAACCCTTTAA‑A‑CCGT‑TGTCAGGATCATATAATAACCCCCCCCGCTGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                      <  1:614050/147‑1 (MQ=255)                                                                                                   cc‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                                     >  1:647208/1‑150 (MQ=255)                                                                                                            tgtTTCTCGAC‑CGTTTAA‑C‑CCGC‑TTCCGGGAACATAGGTCCACCCCCCCCCCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAg                                                <  2:578741/149‑1 (MQ=255)                                                                                                              tATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcgc                                            >  1:383825/1‑151 (MQ=255)                                                                                                              tATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcgc                                            >  1:43106/1‑151 (MQ=255)                                                                                                               aTCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCt                                           >  2:1196282/1‑151 (MQ=255)                                                                                                                 ctcGAC‑CCGTAAA‑C‑CCGC‑TATAAGGATCATATGAACAACCCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                              <  1:201040/146‑1 (MQ=255)                                                                                                                    gAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATg                                      >  1:492313/1‑151 (MQ=255)                                                                                                                        ccGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCCCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc                                    >  1:377835/1‑150 (MQ=255)                                                                                                                         cGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACa                                   >  2:152100/1‑150 (MQ=255)                                                                                                                               aacccGC‑TATCAGGATAAAATGACAACCCCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcg                               <  1:1107184/147‑1 (MQ=255)                                                                                                                               aacccGC‑TATCAGAATCATATGAACACCCCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcg                               <  1:43777/147‑1 (MQ=255)                                                                                                                                aacccttaaTCAGTATAATATAACCAAACCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACa                                   <  1:1234639/138‑1 (MQ=255)                                                                                                                                 cccGC‑TGTCAGGATAATAGAACAAACCCCACCGCCGTTCC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgcg                             <  2:736071/149‑1 (MQ=255)                                                                                                                                   cGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAcc                          >  2:993916/1‑151 (MQ=255)                                                                                                                                   cGC‑TATCAGGATAATATAACCAACCCCCCCCCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAcc                          <  2:539502/151‑1 (MQ=255)                                                                                                                                       tATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGc                       >  1:1071366/1‑151 (MQ=255)                                                                                                                                       tATCAGGATCATATGACCAACCCCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                         <  1:782139/149‑1 (MQ=255)                                                                                                                                          cAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg                    >  1:1370047/1‑151 (MQ=255)                                                                                                                                             gATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCggcc                 >  1:1130856/1‑149 (MQ=255)                                                                                                                                                cATATGACCACCCCCCCCGCCGTGGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAt               <  2:179972/150‑1 (MQ=255)                                                                                                                                                 atatGATCAACCCCCCCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt             <  1:1473238/151‑1 (MQ=255)                                                                                                                                                 atatGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt             >  1:1066838/1‑151 (MQ=255)                                                                                                                                                 atatGACCACCCCCCCCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtg              <  2:696010/150‑1 (MQ=255)                                                                                                                                                 atatGACCACCCCCCCCGCCGATCC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCg                    <  2:1467558/144‑1 (MQ=255)                                                                                                                                                   atGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGc           >  1:501451/1‑151 (MQ=255)                                                                                                                                                     gATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCat         >  2:289028/1‑151 (MQ=255)                                                                                                                                                     gATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCa          >  1:1471128/1‑150 (MQ=255)                                                                                                                                                           cacCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCg   >  1:393870/1‑151 (MQ=255)                                                                                                                                                            acCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCgg  >  1:567917/1‑151 (MQ=255)                                                                                                                                                            |                                                                                                                                                        CTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACC‑GT‑GG‑TGTATCTCGAA‑CCGTTAA‑A‑CCGC‑TATCAGGATCATATGATCAACACCCTCGCCGATGC‑CCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGG  >  NC_000913/1376357‑1376655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑