Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,825,061	0	G	A	39.1%	133.8 / 58.1	87	intergenic (‑124/+79)	ves/spy	cold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (51/2);  new base A (0/34);  total (51/36)
Fisher's exact test for biased strand distribution p-value = 3.77e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGC  >  NC_000913/1824912‑1825208                                                                                                                                                         |                                                                                                                                                    aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGagaaaaga                                                                                                                                                    <  2:586859/151‑1 (MQ=255)   tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGagaaaagaa                                                                                                                                                   >  2:172000/1‑150 (MQ=255)   tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGagaaaaga                                                                                                                                                    >  2:677240/1‑149 (MQ=255)    ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGagaaaagaaa                                                                                                                                                  <  1:913542/150‑1 (MQ=255)       cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAAccg                                                                                                                                              >  1:1163194/1‑151 (MQ=255)         cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAAccgcc                                                                                                                                            <  1:67813/151‑1 (MQ=255)         cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAAccgcc                                                                                                                                            >  2:1047459/1‑151 (MQ=255)          atatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAAccgccg                                                                                                                                           >  1:1183615/1‑151 (MQ=255)            atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGAt                                                                                                                                         >  2:1177137/1‑151 (MQ=255)               aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCt                                                                                                                                      >  1:681943/1‑151 (MQ=255)               aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCt                                                                                                                                      >  1:1248222/1‑151 (MQ=255)               aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCt                                                                                                                                      >  2:115793/1‑151 (MQ=255)                aaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                                     >  2:909401/1‑151 (MQ=255)                aaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTg                                                                                                                                     >  1:1453271/1‑151 (MQ=255)                 aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGt                                                                                                                                    >  2:270191/1‑151 (MQ=255)                    aTTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGCTT‑GAGAAGAGAAAAGAAGACCGCCGATCCTGTcc                                                                                                                                  >  1:1136781/1‑150 (MQ=255)                     ttCCATACCCCCCCCCGGCCCTTTTTTTTCTTTGTTATCGTTCAGGTACCCGTGGATGGTTATCGGCAACTGGGGTGTTTTACTTTAGTGGTGAT‑CAGTTTTTTTTT‑ACAACTTTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTcc                                                                                                                                  <  1:677396/149‑1 (MQ=255)                           aCTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                          >  2:817850/1‑151 (MQ=255)                            cccGCTCCCGGCCTGTTCTTTTCATTGTTATCGTTCAGGTACCGGTGGTTGTTTACGGGAAACTGGGGTGTTTTGCTTTGGTGGTGAC‑GGGGTTTTTTTT‑ACACTTTTTG‑GAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                           <  1:720588/147‑1 (MQ=255)                            cccGCCCCCGCCCTTTTTTTTTTATTTTTATCGTTCAGGTAACGGTGGATGTTTATCGGCAACTGGGGTTATTTACTTTGGTGGTGAC‑CGGTTTTTTTTT‑ACAACTGTTG‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGc                                                                                                                             <  2:924603/145‑1 (MQ=255)                             tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                        >  1:597862/1‑151 (MQ=255)                             tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                        >  1:264857/1‑151 (MQ=255)                              cccccccGGCCCTTTTTTTTTTTTTTTATCGTTCAGGAAACGGTGGATGTTTATCGGCACCTGGGGTGTTTTACTTGAGTGGTGCT‑CAGTTTTTTTTT‑CCACCTGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGc                                                                                                                             <  2:245611/141‑1 (MQ=255)                                 tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                     >  1:578335/1‑150 (MQ=255)                                  cccGGCCTTTTTTTTTAATTTTTATCGTTCAGGAACCGGTGGATGGTTATCGGAACCTGGGGTGATTTAGTTTGGTGGTGAT‑CAGGTTTTTTTT‑ACAACTTTTG‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                       <  1:118532/147‑1 (MQ=255)                                          gTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                           >  1:128794/1‑151 (MQ=255)                                           tttttttCTTTGTTATCGTTCAGGTACCGGTGGTGGTTTATCGGCAACTGGGTTTATTTACTTTGGTGG‑GATCCAGGTTTTTTTA‑ACATTTTTTT‑GAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACt                                                                                                                       <  2:1067134/135‑1 (MQ=255)                                            tCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                         >  1:708804/1‑151 (MQ=255)                                             cTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                         >  1:556108/1‑150 (MQ=255)                                              ttttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                        >  1:123326/1‑150 (MQ=255)                                                       ttATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcgg                                                                                              >  2:1052840/1‑151 (MQ=255)                                                         aTCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc                                                                                            >  1:1018405/1‑151 (MQ=255)                                                            gTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                          >  2:811832/1‑150 (MQ=255)                                                             ttCAGGTAACGGGGGTTGGTTATCGCCAACTCGGGTGATTTACTTTGGTGGTGAT‑CAGTTTTTTTTT‑ACACTTTTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                          <  1:1355159/149‑1 (MQ=255)                                                              tCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCtt                                                                                          >  1:889460/1‑148 (MQ=255)                                                                aGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                     >  2:977355/1‑151 (MQ=255)                                                                 gttAACGGTGGATGGTTATCGGCAACTGGGGTGATTTACTTTAGTGGTGAT‑CAGGTTTTTTTT‑ACAACTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                     <  1:188094/148‑1 (MQ=255)                                                                  gaaccgggggATGTTTATCGGCACCTGGGGTTATTTACGTTAGTGGGGAC‑CAGTTTTTTTTT‑AAAAGTTTTT‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGt                                                                                      <  2:1392133/140‑1 (MQ=255)                                                                  gTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                                   >  1:384993/1‑151 (MQ=255)                                                                    aaCGGTGGAGGTTTACCGGCATCTGGGGTGTTTTACGTTAGTGGAGAC‑CAGGTTTTTTTT‑ACATCTTTTT‑AAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttt                                                                                   <  1:1306215/149‑1 (MQ=255)                                                                    aaCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                                 >  1:107311/1‑151 (MQ=255)                                                                     aCCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                                 >  2:607019/1‑150 (MQ=255)                                                                      cggTGGATGTTTTTCGCCACCGGGGGTTATTTACTTGTGTGGGGAT‑CGGTTTTTTTTA‑ACACTTGTTG‑AAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                          <  2:1456555/138‑1 (MQ=255)                                                                       cGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTgg                                                                               >  1:783349/1‑150 (MQ=255)                                                                        gTGGATGTTTATCGGCACCTCGGGTGATTTACGTTAGTGGTGAT‑CAGGTTTTTTTT‑ACACCTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtt                                                                                     <  1:1052888/143‑1 (MQ=255)                                                                        gTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGGTTTTTGGCt                                                                             >  2:1309841/1‑151 (MQ=255)                                                                        gTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                               >  1:962655/1‑149 (MQ=255)                                                                        gTGGATGGTTATCGGCAACTCGGGCGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGCAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTGAGgttttt                                                                                 >  1:1351676/1‑147 (MQ=255)                                                                         tGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGtttttttggt                                                                             >  2:937813/1‑146 (MQ=255)                                                                              ggTTATCGGCAACGGGGGTGTTTTACTTTGGTGGTGAT‑CGGTTTTTTTTT‑ACAATTTTTA‑AAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                          <  2:1298683/148‑1 (MQ=255)                                                                                            ggggTGTTTTATGTTGGGGGTAAG‑CAGTTTTTTTTA‑ACATCTTTTG‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagc                                                              <  2:662028/145‑1 (MQ=255)                                                                                            ggggTGATTTACTTGAGGGGTGA‑‑CGGGTTTTTTTTAAAAACTGTTG‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagcag                                                            <  2:1086462/147‑1 (MQ=255)                                                                                             gggTGTTTTACGTTAGTGGTGAT‑CAGTTTTTTTTT‑ACACCTTTTG‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                          <  1:312423/149‑1 (MQ=255)                                                                                             gggTAATTTACTTTGGTGTTAAT‑CAGTTTTTTTTT‑AAAATTTTTA‑AAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGtt                                                          <  2:779292/149‑1 (MQ=255)                                                                                              gttgttttattttAGTGGTAAT‑CA‑GTTTTTTTT‑TCAATTTTTTGGAAAAAAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa                                                       <  2:728981/140‑1 (MQ=255)                                                                                               gTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGAAGtt                                                          >  1:172094/1‑147 (MQ=255)                                                                                                   tttcttttAGTGGTAAT‑AAGTTTTTTTTT‑AAACTTGTTG‑AAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAG‑TTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                                   <  1:681870/143‑1 (MQ=255)                                                                                                   tttATGTTGGTGGTGAC‑CAGGTTTTTTTT‑ACAACTGTTG‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                                   <  1:1162479/150‑1 (MQ=255)                                                                                                   tttACTTTGGTTGTGAT‑C‑GGCTTTTTTT‑ACCACTTTTTAAAAAAGAAAAAAAAAATCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                                   <  1:727231/150‑1 (MQ=255)                                                                                                   tttACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTACGATTTTTTGTTCGAAGAATTTTCCGCAGGTGccg                                                      >  2:637566/1‑145 (MQ=255)                                                                                                      aCGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCTTTAAGTTTTTTGGCTGAAAGATTATTCCGCAGGTGGAGGGAtttt                                               >  2:602888/1‑151 (MQ=255)                                                                                                       cGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCAtttt                                               >  2:1091271/1‑150 (MQ=255)                                                                                                        actttggtggatatcggCTTTTTTTCATAATTTTTT‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                                <  1:458827/135‑1 (MQ=255)                                                                                                             ggggataccctgtttttttt‑ACAACTTTTG‑GAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGTACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                          <  2:1375743/137‑1 (MQ=255)                                                                                                              ggtgAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTTATTTTTTTTGTTGAAAGGTTATTAACACGTTGCCGGCCTTTTTCCtttt                                        >  2:972927/1‑150 (MQ=255)                                                                                                               ggtgtgcggttttttttt‑ACATTTGTTG‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                        <  1:719386/139‑1 (MQ=255)                                                                                                                  aT‑CAGGTTTTTTTT‑ACATTTTTTG‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTg                                       <  2:1183557/147‑1 (MQ=255)                                                                                                                     cAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCGc                                   >  1:33981/1‑149 (MQ=255)                                                                                                                     cAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAAATTATTCAGCAGTTGCAGGCATTTTTCCTTTTGCCGCTg                                 >  1:974956/1‑151 (MQ=255)                                                                                                                      aGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                                >  1:1141634/1‑151 (MQ=255)                                                                                                                       gttttttttt‑ACAACTTTTG‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCt                                  <  1:1346133/145‑1 (MQ=255)                                                                                                                       gtttttttta‑ACACCTTTTG‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGa                               <  1:766440/148‑1 (MQ=255)                                                                                                                       ggtttttttt‑ACATTTGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                          <  2:488096/137‑1 (MQ=255)                                                                                                                           tttttt‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                           >  1:572059/1‑151 (MQ=255)                                                                                                                            ttttt‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGGCGGCATTTTACCTTTTTCCGCTGGACg                             >  2:1001663/1‑148 (MQ=255)                                                                                                                             tttt‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGAAGTGGACAAGACCGGCGGTCTTAAGCTTTTTCGCTGAAGCGTTATTTAACAATTGCAGGCATTTTTACTTTTGCCGCTGGGCggt                           >  2:1450343/1‑147 (MQ=255)                                                                                                                                  aCAGCTTTTG‑GAGAAAAGAAAGGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                     <  1:1446964/151‑1 (MQ=255)                                                                                                                                  aCAAGTGTTA‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                      <  1:33319/150‑1 (MQ=255)                                                                                                                                   cAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGACTTTAGGTTTTTGGGTGAAAGAATTTTTAGCAGTTGCAGGCATTTTACCTTTTGGCCCCGGACGTTccttc                      >  2:306894/1‑145 (MQ=255)                                                                                                                                    aaCTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                      >  2:197532/1‑148 (MQ=255)                                                                                                                                       cttttagaaaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                      <  2:1398159/137‑1 (MQ=255)                                                                                                                                        gTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc                >  2:139635/1‑150 (MQ=255)                                                                                                                                          ttagaaaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                  <  1:811853/141‑1 (MQ=255)                                                                                                                                          tt‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt              >  1:820531/1‑150 (MQ=255)                                                                                                                                              agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGGCTTTTAACTTTTGTCGCTGGACGTTCTGTCAGACGCTtc             >  2:1119862/1‑148 (MQ=255)                                                                                                                                              agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTtctc           >  1:793067/1‑150 (MQ=255)                                                                                                                                              agaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc           <  1:172002/150‑1 (MQ=255)                                                                                                                                              aaaaaaaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtct            <  2:112441/142‑1 (MQ=255)                                                                                                                                                aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCCTTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCaa         >  2:447291/1‑150 (MQ=255)                                                                                                                                                aagaaaaAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa        <  1:1253039/151‑1 (MQ=255)                                                                                                                                                aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTGCCTTTTGCCGCTGGACGTTCTGTCAGACGCTtc             <  2:407691/146‑1 (MQ=255)                                                                                                                                                 agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa        >  1:1138340/1‑150 (MQ=255)                                                                                                                                                   agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt     >  1:327451/1‑151 (MQ=255)                                                                                                                                                     aaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTa    <  2:1468035/150‑1 (MQ=255)                                                                                                                                                     aaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTa    <  2:1224919/150‑1 (MQ=255)                                                                                                                                                     aaaaaaaaaCCGCCGATCCTGTCCACCGCAATACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAg   <  1:819122/151‑1 (MQ=255)                                                                                                                                                      aaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGc  <  1:630610/151‑1 (MQ=255)                                                                                                                                                         |                                                                                                                                                    ATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGAT‑CAGGCTTTTTTT‑ACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGC  >  NC_000913/1824912‑1825208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑