Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,903,6920CA38.6% 137.3 / 55.7 83intergenic (+318/+1055)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base C (6/45);  new base A (32/0);  total (38/45)
Fisher's exact test for biased strand distribution p-value = 2.86e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

GTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAA  >  NC_000913/2903542‑2903839
                                                                                                                                                      |                                                                                                                                                        
gTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAc                                                                                                                                                          <  2:47919/151‑1 (MQ=255)
gTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAc                                                                                                                                                          <  2:331690/151‑1 (MQ=255)
   tttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACa                                                                                                                                                        >  2:1443902/1‑150 (MQ=255)
   tttACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCa                                                                                                                                                        <  1:1048272/150‑1 (MQ=255)
     tACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACt                                                                                                                                                      <  1:960313/150‑1 (MQ=255)
         aGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTa                                                                                                                                               <  2:373626/151‑1 (MQ=255)
           gAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGc                                                                                                                                             <  2:88087/151‑1 (MQ=255)
           gAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGc                                                                                                                                             <  2:828955/151‑1 (MQ=255)
              tatTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACCCCCATTAGcccc                                                                                                                                          >  1:1030193/1‑149 (MQ=255)
                  aaCTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACaacccctaaactc                                                                                                                                            >  1:177366/1‑135 (MQ=255)
                      acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGCACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTGATTTTAAAACACCCAACAGAcacaacacaa                                                                                                                                   >  2:1396049/1‑150 (MQ=255)
                      acacTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACaaa                                                                                                                                  <  1:480172/151‑1 (MQ=255)
                          tCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACAACCATTATTCTCCAaaaaaaaa                                                                                                                                >  1:234152/1‑147 (MQ=255)
                           cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACaa                                                                                                                             <  1:44419/151‑1 (MQ=255)
                           cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACaa                                                                                                                             <  1:628823/151‑1 (MQ=255)
                           cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACa                                                                                                                              <  2:121635/150‑1 (MQ=255)
                            gATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACaaa                                                                                                                            <  1:872090/151‑1 (MQ=255)
                               gTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGGTATGTTAAAAAACACATATTTCCTCaaaaaaaaaaaaaa                                                                                                                            >  2:1270644/1‑149 (MQ=255)
                                   ttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTc                                                                                                                        <  1:1418582/148‑1 (MQ=255)
                                   ttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCa                                                                                                                      <  2:63536/150‑1 (MQ=255)
                                      gAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAtt                                                                                                                <  1:1344846/151‑1 (MQ=255)
                                       aaTAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTACAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGATTTGTCAAAACAATCTTTTTCCTCAAaaccaaaaccaaacaatcaaatt                                                                                                                 >  2:1200559/1‑140 (MQ=255)
                                          aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATttatt                                                                                                            <  2:1336778/151‑1 (MQ=255)
                                           aaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAAAAAACATTAATCTCaaaaaaaaaaaaaaaattcaaattt                                                                                                                 >  2:14508/1‑136 (MQ=255)
                                           aaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACAATCATTATGCTCCAAAAAAAAACaaaaattccaagttaat                                                                                                              >  2:654065/1‑136 (MQ=255)
                                                    aCGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAAACAATTAAACTCAAAAAAAAAAAAAAAATCCAGATTTTTTACTTaa                                                                                                      >  2:56553/1‑147 (MQ=255)
                                                      gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACCCTCATTTTACCCAAAAAAAAAAAAAAATTTCCGAATTTTTTATTTAAcaa                                                                                                   >  1:689914/1‑147 (MQ=255)
                                                      gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTATAAAAACACACAATATGCTCAAAAAAAAAAAAAAAATCCAACTTTTTTTCtttaataatg                                                                                                >  2:725506/1‑143 (MQ=255)
                                                       aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACCCCTTAACCTCCAAAAAAAAAAAAAAATCCACATTTTTTAATTAAACaaa                                                                                                >  1:1131856/1‑148 (MQ=255)
                                                       aaTCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGa                                                                                              <  1:1354300/151‑1 (MQ=255)
                                                         tCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAAAACTCATTTACCTCCAAAAAAAAAAAAAAATCCAGAATTTTTTTTTTAACAAAtaa                                                                                              >  2:324875/1‑148 (MQ=255)
                                                          cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAAt                                                                                            <  2:594557/150‑1 (MQ=255)
                                                          cGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATa                                                                                           <  2:1330393/151‑1 (MQ=255)
                                                           gCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATaa                                                                                          <  1:1214268/151‑1 (MQ=255)
                                                             aCTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACTCCTTAACCTCAAAAAAAAAAAAAAATTCCCGATTTTTTTATTTACCAAAGAATaa                                                                                          >  1:1151526/1‑149 (MQ=255)
                                                                ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAAAACACCTTATATCCAAAACAAAAAAAAAATTCCACATTTTTTTTTTTACCAAAGaaaaaaaa                                                                                       >  1:161326/1‑149 (MQ=255)
                                                                ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACACAATAACCTCAAAAAAAAAAAAAAATTTCAAAATTATTTATTAACCaactaaaaaaaaa                                                                                      >  1:1438402/1‑139 (MQ=255)
                                                                ttATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACaa                                                                                       <  1:706388/149‑1 (MQ=255)
                                                                  aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAAAACTCCTTTTCCTCAAAAAAAAAAAAAAAAACCAAATTTATTATTTAAACaaaaaaaaaaaaaaat                                                                                   >  2:655607/1‑137 (MQ=255)
                                                                     aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACCCATTAATCTCCAAACAAAAAAAAACATCCAGACTTAATTCTTAAACAAATAATAaaaaaaataa                                                                                 >  2:67396/1‑144 (MQ=255)
                                                                           cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATAAATAACAAGCTTAAAAAatat                                                                          <  2:674984/151‑1 (MQ=255)
                                                                              ttAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAAAACCCATTTTCCTCAAAAAAAAAACAAAATTCCCGATTTTTTTTTTTAACCAATAATAAAAAACCTTaaaaaaa                                                                            >  1:336471/1‑146 (MQ=255)
                                                                               tAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACACCCACTTACCCCCAAAAAAAAAAAAAAATTCCAATTTTTTTCTTAAACCAAGAAAAAAAAACATAAAAAAaataatt                                                                      >  1:1411371/1‑148 (MQ=255)
                                                                                aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAAAACTCATTAACCTCAAAAAAAAAAAGAAAATTCAGAATTATTAATTAAAAAAAGAAAAAAAAAAATaaaaaaaaaaa                                                                        >  2:37630/1‑143 (MQ=255)
                                                                                aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACt                                                                       <  1:1107624/149‑1 (MQ=255)
                                                                                aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGt                                                                     <  2:886039/151‑1 (MQ=255)
                                                                                  aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACATATTATACTCAAAAAAAAAAAAAAATTCCACAATTATTATTTTAACAAAAAAAAAAAAACTTaaaaaa                                                                             >  2:1178092/1‑141 (MQ=255)
                                                                                  aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTa                                                                   <  2:1473742/151‑1 (MQ=255)
                                                                                     aTGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTc                                                                <  2:1042690/151‑1 (MQ=255)
                                                                                       gAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCt                                                               <  2:1170006/150‑1 (MQ=255)
                                                                                            ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACTCAATATCCACCAAAAAAAAAAAAAAATTCAGATTTTTTTTTTAAACAAAGAAAAACAAAGCTAAAAAAAAAAATGTTAttt                                                                >  1:832429/1‑143 (MQ=255)
                                                                                            ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCGCTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAg                                                           <  1:400124/149‑1 (MQ=255)
                                                                                            ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAg                                                           <  1:201611/149‑1 (MQ=255)
                                                                                             gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaa                                                        <  2:262422/151‑1 (MQ=255)
                                                                                                 tCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACACACAATATGCGCAAAAAAAAAAAAAAATTCCCGATTTTTTAATTTACCAAAAAAAAAAAAACATCAAAAAAATAATATTATTCTaaaaaaaaaa                                                     >  1:266090/1‑150 (MQ=255)
                                                                                                    ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaaaaa                                                     <  1:1249835/147‑1 (MQ=255)
                                                                                                    ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTa                                                  <  2:768800/150‑1 (MQ=255)
                                                                                                    ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTa                                                  <  1:77466/150‑1 (MQ=255)
                                                                                                     tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTaa                                                 <  1:625829/150‑1 (MQ=255)
                                                                                                      aTTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAAACCATTTTTCTCCAAAAAAAAAAAAAAATCCACAGTTTTTTTTTTAACAAATAATAAAAAAAATTAAAAAAAATATTTTTTTTTTAAAAAAAAAAtaa                                                  >  2:236077/1‑147 (MQ=255)
                                                                                                         cATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACCCACTAACCTCAAAAAAAAAAAAAAAATCCCGATTTTTTTATTTAACAAAAAATAAAAAAAATAAAAAAAAAAATTTTATTTTTAAAAAAAAAATaaa                                                >  2:108394/1‑146 (MQ=255)
                                                                                                         cATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAAAACCCATTTTCCTCAAAAAAAAAAAAAAAATTCCGATTTTTTACTTTACCAAATAAAAAAAAACTTTAAAAAAAAAATTTTTTTTTTaaaaaaaaaaaaaaa                                                >  1:1248297/1‑143 (MQ=255)
                                                                                                          aTAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAAAACTCCTTAACCTCAAAAAAAAAAAAAAAATTCCGATTTTTTAATTTAACAAAGAAAAAAAAAAATAAAAAAAATAATTTTTTTTTTAAAAAAAAAATaaaa                                               >  1:505390/1‑145 (MQ=255)
                                                                                                             aGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAACACACCTTATCCTCCAAAAAAAAAAAAAAATCCAGATTTTTTACTTTACCAAAGAAAAAAAAACTTAAAAAAAATAATGTTATTTTTAAAAAAAAAATAAAAAGCAtt                                         >  1:347191/1‑150 (MQ=255)
                                                                                                             aGAACTATTCATCAACAGCGTATAGAGGCGGCTATGGAAAAACACTCATTTTACCCCAAACAAAAAAAAAAATTCAGATTTATTTCTTTACCAAAGAACAACAAACTTTAAAAAAATACTGTTTTTCTCAAAAAAAAAATAAAAAGCAtt                                         >  1:1243582/1‑150 (MQ=255)
                                                                                                               aaCTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCCTTAACCTCAAAAAAAAAAAAAAAATCCCGATTTATTTATTTAACAATTAAAAAAAAACATAAAAAAAATAATTTTTTTTTAAAAAAAAAAATAAAAAACATTaa                                       >  2:833883/1‑150 (MQ=255)
                                                                                                                      catcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAatat                               <  2:849488/151‑1 (MQ=255)
                                                                                                                      catcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTAAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAata                                <  2:604474/150‑1 (MQ=255)
                                                                                                                         caACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaa                            <  1:56553/151‑1 (MQ=255)
                                                                                                                         caACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTa                             <  2:1180235/150‑1 (MQ=255)
                                                                                                                                  ataGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataat                   <  2:387344/151‑1 (MQ=255)
                                                                                                                                  ataGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataat                   <  2:294848/151‑1 (MQ=255)
                                                                                                                                        gCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa             <  1:67396/151‑1 (MQ=255)
                                                                                                                                         cGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa             >  2:874797/1‑150 (MQ=255)
                                                                                                                                         cGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATCACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaa            <  1:1393884/151‑1 (MQ=255)
                                                                                                                                          ggTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGTCATTAATTCAATATTAAAAAAATAATAGATTaaa           <  2:1351603/151‑1 (MQ=255)
                                                                                                                                           gTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCATATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaaa          <  2:430428/151‑1 (MQ=255)
                                                                                                                                           gTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTaaa           >  1:344994/1‑150 (MQ=255)
                                                                                                                                             tATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAtt        >  2:989060/1‑151 (MQ=255)
                                                                                                                                             tATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAtt        >  2:798808/1‑151 (MQ=255)
                                                                                                                                              aTGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAttt       >  2:721864/1‑151 (MQ=255)
                                                                                                                                                  aaaaCCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCtt    >  2:342316/1‑150 (MQ=255)
                                                                                                                                                   aaaCCACTCATTAGCCTCAAAACAAAGACACACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTaa  <  1:803697/151‑1 (MQ=255)
                                                                                                                                                   aaaCCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTaa  <  1:232596/151‑1 (MQ=255)
                                                                                                                                                   aaaCCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTaa  <  1:393487/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                        
GTTTTTACAAGGAATATTAACTACACTCGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAA  >  NC_000913/2903542‑2903839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: