Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT39.7% 90.9 / 30.9 68S62F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (9/32);  new base T (27/0);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 1.86e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604198‑4604492
                                                                                                                                                  |                                                                                                                                                     
cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttt                                                                                                                                                   <  1:64612/151‑1 (MQ=255)
cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                                     <  2:817054/149‑1 (MQ=255)
 aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttt                                                                                                                                                  >  2:160375/1‑151 (MQ=255)
 aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttt                                                                                                                                                  >  1:538129/1‑151 (MQ=255)
   aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttttc                                                                                                                                                 <  1:1377863/150‑1 (MQ=255)
   aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                                <  2:443951/151‑1 (MQ=255)
    gAAAAATGCGTCGTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttt                                                                                                                                                  >  1:683751/1‑148 (MQ=255)
     aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGc                                                                                                                                              <  1:1410347/151‑1 (MQ=255)
      aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCtttttttttttttttcc                                                                                                                                             >  2:1362026/1‑146 (MQ=255)
      aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttt                                                                                                                                                >  2:1105429/1‑146 (MQ=255)
       aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCa                                                                                                                                            <  1:276303/151‑1 (MQ=255)
        aaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttttcc                                                                                                                                             >  2:406892/1‑144 (MQ=255)
         aTGCGTCATGAGTAATTTCCTTATTCAGAGTTTATTCAGAAATTTTGCGGGTAACCCTTATAAGCTCCATACCTATATCAGACAGTACTCATTTCAGGATGCTATGCCGCGGATCTCGTTTGCGGCGGTCATTTTCGCTTTTACTg                                                                                                                                               <  2:1021319/146‑1 (MQ=255)
           gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  2:829948/151‑1 (MQ=255)
           gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga                                                                                                                                         <  1:1145094/150‑1 (MQ=255)
               cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                                    <  2:1133304/151‑1 (MQ=255)
                aagAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATAGCAGTCAGCAGTCATTTCAGGAAGCCATGTCGCGGATCCCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCACGAGAAGt                                                                                                                                    <  2:776118/148‑1 (MQ=255)
                  gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGa                                                                                                                                  <  1:1074678/150‑1 (MQ=255)
                  gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                                 <  1:502583/151‑1 (MQ=255)
                           cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagagag                                                                                                                        <  1:757283/151‑1 (MQ=255)
                            ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGACATTTTTTTTTTTTTTGCCCTTTGAAAGGAGCGCagcgag                                                                                                                        >  2:399851/1‑150 (MQ=255)
                              tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCATGAGAAGGGGGGGCCGAGAggg                                                                                                                      >  2:689740/1‑150 (MQ=255)
                                  gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                  <  2:1400819/150‑1 (MQ=255)
                                  gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                                                                                                  <  2:1037595/150‑1 (MQ=255)
                                     agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTTCATGTGAAGTGAGGGCAGAGAGGGGTTAtttt                                                                                                              >  2:216850/1‑151 (MQ=255)
                                     agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGGGAGGGGTTAtttt                                                                                                              >  2:1119075/1‑151 (MQ=255)
                                          aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGGTATTTTTCct                                                                                                          >  2:620712/1‑150 (MQ=255)
                                           ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTGCCATGAGAAAGGAGGGGAGGGAGGGAttttttt                                                                                                              >  1:1031310/1‑140 (MQ=255)
                                           ttCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTGAGAAGTGAGCGCAGAGAGGGGTTATTTTGcc                                                                                                           >  2:880463/1‑148 (MQ=255)
                                              aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGGAGTGGGCGCAGAGGGGGGTTATCTTGcc                                                                                                           >  2:321099/1‑145 (MQ=255)
                                               gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCAATAGAAGGGAGCGCGGAGAGGGaatttttt                                                                                                              >  2:318309/1‑134 (MQ=255)
                                               gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgactga                                                                                                    <  1:365461/151‑1 (MQ=255)
                                                  aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTATCTTTGCTGACTGAAc                                                                                                  >  2:1207385/1‑150 (MQ=255)
                                                  aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGCGCAGAGAGGGGTTATTTTTCCTGACTGAAc                                                                                                  >  1:989287/1‑150 (MQ=255)
                                                     ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTgg                                                                                               <  2:720830/150‑1 (MQ=255)
                                                     ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTAATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTgg                                                                                               <  1:1105398/150‑1 (MQ=255)
                                                             aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGGGCCGAGAGGGATTATCTTTCCTGACTGAACTGGCGGttta                                                                                        >  1:1073869/1‑147 (MQ=255)
                                                               cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCCTGAGAAGGGGGGGCAGAGAGGGATTATCTTGCCTGAATGAAATGGCGGTTAAGgtt                                                                                    >  1:214885/1‑151 (MQ=255)
                                                                ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCGTTTTTTTTTTTTTTTCCAAGAGAGGGGGGCGCGGGGGGGGGTTATTTTGTCTGGTTGTACACGCGAttta                                                                                        >  2:470476/1‑144 (MQ=255)
                                                                    ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTAGAAGTGAGCGCAGAGGGGGGTTATTTTTCCTGCCTGAACTGGCGATTAAGGTTTcgc                                                                                >  2:621204/1‑150 (MQ=255)
                                                                    ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCcgc                                                                                <  1:1170492/150‑1 (MQ=255)
                                                                      aaGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGt                                                                              <  2:77586/150‑1 (MQ=255)
                                                                      aaGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGt                                                                              <  1:1003974/150‑1 (MQ=255)
                                                                      aaGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTa                                                                             <  2:1004903/151‑1 (MQ=255)
                                                                       aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGGGCGGAGAGGGATTTTTTTTCCTGACTGAACTGGGGGATAAGGTTcccc                                                                                >  1:904476/1‑145 (MQ=255)
                                                                        gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGTTATTTTTCCTGACTTGACTGGCGATTAAGTTTTCGCGTAcc                                                                           >  2:436890/1‑151 (MQ=255)
                                                                          tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGCGCGCAGAGAGGGGTTTTTTTTCCTTACTGGACTTGCGGTTAAAGTTTCGCGTTccc                                                                          >  2:910079/1‑150 (MQ=255)
                                                                           ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGGTATCTTGCCTGACTTAACTGGCGATTAAGTTTTCGCGTACCCgg                                                                        >  1:737831/1‑151 (MQ=255)
                                                                           ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                                                                          <  1:1252438/149‑1 (MQ=255)
                                                                             aTACCTATGCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCg                                                                     <  1:1037012/151‑1 (MQ=255)
                                                                              tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGGGAGGGATTATCTTTCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCg                                                                     >  1:455791/1‑150 (MQ=255)
                                                                                 cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGGAGTGAGGGCAGAGAGGGATTATTTTTCCTGACTGAACTGGCGGTTTAGTTTTCCCGTACCCGGCGttt                                                                  >  1:924267/1‑150 (MQ=255)
                                                                                   ataCCAGTCAGGAGTCATTTCAGGATGCTATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTa                                                                 <  1:821228/149‑1 (MQ=255)
                                                                                   ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGGGGGGAGGGGGGGGTTATTTTTTCTTACTGAACTGGGGGTTAAGGTTCCGCGTACCCGGCGGTTTGt                                                               >  1:289568/1‑151 (MQ=255)
                                                                                      ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAt                                                            <  1:1036849/151‑1 (MQ=255)
                                                                                             ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                                     <  2:1361490/151‑1 (MQ=255)
                                                                                                 tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCATTTTTTTTTTTCTGGCACGAAAAGTGGGGGGAGAGAGGGAATATTGTGTCTGGCTGAACTTGTGATTAAAGTTCCCCGTAACCGGCGGTTTGTtttttgcgg                                                      >  1:589167/1‑141 (MQ=255)
                                                                                                  cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGCTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                                 >  2:297557/1‑150 (MQ=255)
                                                                                                   aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTCCCCGGCGTTTAGTTATTGCGgatgatg                                                 <  2:217648/149‑1 (MQ=255)
                                                                                                   aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                                <  2:258063/150‑1 (MQ=255)
                                                                                                     ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGata                                              <  2:1247586/150‑1 (MQ=255)
                                                                                                      tgaggctcacATGTCGCGGATCTCGTTTGCGACGATCGTTTTTGCTTTTTCTGACATGAGAAGTGAGGGCAGAGAGGGATTGTCGTGCCTCACTGCACTGGCGATAAAGTTTCCGCGTTCCCGGCGTTCAGTCATTGCGGATGGTGatat                                             <  2:871328/141‑1 (MQ=255)
                                                                                                       cAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATAtt                                            <  2:586329/150‑1 (MQ=255)
                                                                                                        aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCACGGGGAGAGAGAGGCGTGAGGGAGTATCTTTACTGACTGAACAGGGGATTAAGTTTTCGCCCAACAGGCGTTTAGTTTTTGTGggagatgtaatt                                            >  1:361828/1‑144 (MQ=255)
                                                                                                         ggATGCCATGTCGCGGATCTCGTTTGCGGCGGCCCTTTTTTTTTTTTCTGCCCTGCGGAGGGGGCGGCGAGGGGGGTTATCTTGGCCGACTTAACTGGCGGTTTATTTTTCCCGGACCCGGCGCTTTGGTATTGGGGGTGATGGTAtt                                            >  2:1127779/1‑148 (MQ=255)
                                                                                                          gATGCCATGTCGCGGATCTCGTTTGCGGCGGTTATTTTTTTTTTTTTTTCCGTGGGAAGGGAGAGCCGGGAGGGGGTTTCTTTTCCGACTGAAATGGAGATTATATTTTCGCGGGCCCGGCGGTTTATTTTTTgggggtatgaattt                                             >  2:1479185/1‑137 (MQ=255)
                                                                                                               cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCgg                                   <  1:427080/151‑1 (MQ=255)
                                                                                                                 tGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGGAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGCGGTTTGTTATTGCGGGTGATGATATTTAAGCTCgg                                   >  1:490589/1‑149 (MQ=255)
                                                                                                                    cgcgGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGGGAGGGCAGAGAGGGGTTTTCTTGCCTGACTGAAATGGCGGTTAAGTTTCCGCGTACCCCGCGGTTAGTTTTTTCGGGTGATGATATTGAAGCTCgg                                   >  1:80757/1‑146 (MQ=255)
                                                                                                                       ggATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGGTTATTTTTCCTGACTGAAATTGCGGTTAAGTTTCCGCGGACCCGGCGGTTTGTTATTTCGGGTGGTGATATTTAAGCTCCGCTGGtttg                           >  1:1182466/1‑149 (MQ=255)
                                                                                                                             cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa                       <  2:204076/149‑1 (MQ=255)
                                                                                                                                   cggcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGcc               >  1:695220/1‑151 (MQ=255)
                                                                                                                                     gcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt             >  2:89043/1‑151 (MQ=255)
                                                                                                                                      cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc            <  2:1218839/151‑1 (MQ=255)
                                                                                                                                          cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAcc         >  1:135698/1‑150 (MQ=255)
                                                                                                                                          cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg        >  1:801101/1‑151 (MQ=255)
                                                                                                                                           aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg        >  2:7846/1‑150 (MQ=255)
                                                                                                                                           aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc       >  2:898942/1‑151 (MQ=255)
                                                                                                                                             tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt      <  1:960992/150‑1 (MQ=255)
                                                                                                                                              ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTg     <  1:462583/150‑1 (MQ=255)
                                                                                                                                                ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa   <  2:361823/150‑1 (MQ=255)
                                                                                                                                                ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGa   >  1:622101/1‑150 (MQ=255)
                                                                                                                                                ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  >  2:352550/1‑151 (MQ=255)
                                                                                                                                                ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCACTgg    >  2:1226915/1‑149 (MQ=255)
                                                                                                                                                 tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  <  1:486642/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604198‑4604492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: