Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,968,163	Δ1 bp	coding (272/2247 nt)	ptsP ←	PEP‑protein phosphotransferase enzyme I; GAF domain containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,968,160	0	T	.	100.0%	360.8 / NA	75	coding (275/2247 nt)	ptsP	PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (44/31);  total (44/31)

AAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATG  >  NC_000913/2968010‑2968302                                                                                                                                                       |                                                                                                                                               aaggaTTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCt                                                                                                                                                >  1:958653/1‑151 (MQ=255)   ggaTTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCttt                                                                                                                                              >  1:950910/1‑151 (MQ=255)   ggaTTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCttt                                                                                                                                              >  2:144432/1‑151 (MQ=255)   ggaTTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCttt                                                                                                                                              >  1:1307113/1‑151 (MQ=255)       tcttcACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCtttgcg                                                                                                                                           <  1:1332920/150‑4 (MQ=255)       tcttcACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCtttgcg                                                                                                                                           <  1:130608/150‑4 (MQ=255)         ttcACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCAt                                                                                                                                       >  2:720920/1‑151 (MQ=255)          tcACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCtttgcgc                                                                                                                                          <  1:1145713/148‑5 (MQ=255)          tcACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCAt                                                                                                                                       <  1:1077732/150‑1 (MQ=255)            aCTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTg                                                                                                                                    <  2:370450/151‑1 (MQ=255)                 cGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCCTCTGCAAgg                                                                                                                               >  2:221335/1‑151 (MQ=255)                 cGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAgg                                                                                                                               <  1:904707/151‑1 (MQ=255)                    cATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGGTGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGatt                                                                                                                            >  2:397001/1‑151 (MQ=255)                    cATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGttt                                                                                                                            >  2:352847/1‑151 (MQ=255)                    cATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGtt                                                                                                                             <  1:1014384/150‑1 (MQ=255)                     aTACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGttt                                                                                                                            >  1:692117/1‑150 (MQ=255)                       aCTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTAt                                                                                                                          <  1:291574/150‑1 (MQ=255)                       aCTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATc                                                                                                                         <  2:13461/151‑1 (MQ=255)                        cTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCg                                                                                                                        >  2:87240/1‑151 (MQ=255)                          ggCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCg                                                                                                                        <  1:877997/149‑1 (MQ=255)                          ggCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGt                                                                                                                      <  2:774580/151‑1 (MQ=255)                               aaCTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGc                                                                                                                 >  1:663292/1‑151 (MQ=255)                                aCTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGcc                                                                                                                >  1:885843/1‑151 (MQ=255)                                  tctcGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGcc                                                                                                                >  2:1288540/1‑149 (MQ=255)                                           tGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGATTATCGGTTCCGCCAGCCTGCCAAc                                                                                                     >  2:85141/1‑151 (MQ=255)                                                  ccaGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGcc                                                                                               <  1:398249/150‑1 (MQ=255)                                                  ccaGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCcg                                                                                              <  2:851315/151‑1 (MQ=255)                                                   caGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCcg                                                                                              >  1:650193/1‑150 (MQ=255)                                                    aGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCcgac                                                                                            >  2:1276083/1‑151 (MQ=255)                                                       acacCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGAt                                                                                         <  1:1239393/151‑1 (MQ=255)                                                            aaGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCtt                                                                                    >  2:311860/1‑151 (MQ=255)                                                            aaGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCT‑GGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTtc                                                                                   >  1:732169/1‑151 (MQ=255)                                                              gCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTtca                                                                                  <  1:525954/151‑1 (MQ=255)                                                               cAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCACATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTtcat                                                                                 >  1:1232101/1‑151 (MQ=255)                                                                    ggCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAAc                                                                            >  2:738879/1‑151 (MQ=255)                                                                      cGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAAcgc                                                                          >  2:750079/1‑151 (MQ=255)                                                                       gACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAAcgcg                                                                         >  2:801703/1‑151 (MQ=255)                                                                          gTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGc                                                                      >  1:343699/1‑151 (MQ=255)                                                                            tGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCg                                                                     <  1:1083423/150‑1 (MQ=255)                                                                             gAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGtt                                                                   <  2:957117/151‑1 (MQ=255)                                                                                taatTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTAc                                                                 >  1:784746/1‑150 (MQ=255)                                                                                 aatTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGCCGATCCCTTCATCAAACGCGAGCGTTAc                                                                 >  1:789009/1‑149 (MQ=255)                                                                                 aatTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTAc                                                                 >  2:498117/1‑149 (MQ=255)                                                                                 aatTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTAc                                                                 >  2:685694/1‑149 (MQ=255)                                                                                   ttGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTg                                                             >  1:776024/1‑151 (MQ=255)                                                                                    tGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGc                                                            >  1:292866/1‑151 (MQ=255)                                                                                     ggTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCg                                                           <  1:417536/151‑1 (MQ=255)                                                                                         cGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATAAATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGAGcg                                                       >  2:370151/1‑151 (MQ=255)                                                                                             tAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTg                                                   >  1:1239854/1‑151 (MQ=255)                                                                                                 aaCGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGttt                                               >  1:1161200/1‑151 (MQ=255)                                                                                                     cgcgGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTc                                           <  2:375529/151‑1 (MQ=255)                                                                                                      gcgGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCa                                          <  2:1012080/151‑1 (MQ=255)                                                                                                      gcgGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCa                                          <  2:1323720/151‑1 (MQ=255)                                                                                                        ggAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGc                                        >  1:755542/1‑151 (MQ=255)                                                                                                          aaaCGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGccc                                      <  2:485950/151‑1 (MQ=255)                                                                                                           aaCGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGcccc                                     >  1:1241296/1‑151 (MQ=255)                                                                                                           aaCGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGcccc                                     >  1:236724/1‑151 (MQ=255)                                                                                                             cGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCg                                   <  2:439069/151‑1 (MQ=255)                                                                                                              gTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCgg                                  >  1:65853/1‑151 (MQ=255)                                                                                                                    cttTTACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTGGCGCATCTGCAGGGTTTATCGGTTCCCCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGGGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTc                               <  1:1134255/148‑1 (MQ=255)                                                                                                                        tACGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATc                         <  2:730723/150‑1 (MQ=255)                                                                                                                         aCGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATc                         >  2:129041/1‑149 (MQ=255)                                                                                                                         aCGGAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATc                         >  2:308356/1‑149 (MQ=255)                                                                                                                           ggAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAgg                      >  2:1315181/1‑150 (MQ=255)                                                                                                                            gAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGgta                    <  2:1035463/151‑1 (MQ=255)                                                                                                                            gAGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGgt                     >  1:585768/1‑150 (MQ=255)                                                                                                                             aGGGGATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGgtag                   <  1:1005824/151‑1 (MQ=255)                                                                                                                                 gATGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTaac               >  2:1002092/1‑151 (MQ=255)                                                                                                                                  aTGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTaaca              <  1:357658/151‑1 (MQ=255)                                                                                                                                  aTGTATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTaac               >  1:225580/1‑150 (MQ=255)                                                                                                                                     tATTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTaacaa             <  1:1016707/149‑1 (MQ=255)                                                                                                                                      aTTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACg           >  2:244917/1‑150 (MQ=255)                                                                                                                                      aTTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACg           <  1:672340/150‑1 (MQ=255)                                                                                                                                      aTTTGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGt          >  1:710699/1‑151 (MQ=255)                                                                                                                                        ttGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTc         <  1:352855/150‑1 (MQ=255)                                                                                                                                         tGAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCg        >  1:986790/1‑150 (MQ=255)                                                                                                                                          gAAGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGa       <  1:668351/150‑1 (MQ=255)                                                                                                                                            aGCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCa    <  2:1048671/151‑1 (MQ=255)                                                                                                                                             gCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCAt   >  2:548614/1‑151 (MQ=255)                                                                                                                                             gCTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCAt   <  1:1236679/151‑1 (MQ=255)                                                                                                                                              cTGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATg  >  2:403863/1‑151 (MQ=255)                                                                                                                                               tGGGGTGC‑TTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATg  >  1:110661/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                               AAGGATTCTTCACTTTCGTCATACTGGCGCAACTCTCGTTGCTGTACCACCAGTACACCAAGCAACTGGCGACGTTGAATAATTGGTACGCCTAAAAACGCGCGGAAACGTTCTTCTTTTACGGAGGGGATGTATTTGAAGCTGGGGTGCTTTTGCGCATCTGCAAGGTTTATCGGTTCCGCCAGCCTGCCAACCAGGCCGACGATCCCTTCATCAAACGCGAGCGTTACAGTGCGACCGCGTGGTTTTTTCAGCCCCCGGGTCGCCATCAGGTAGTAACAACGTCGATCATG  >  NC_000913/2968010‑2968302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑