Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,039	0	T	G	41.7%	98.4 / 15.2	60	N108T (AAC→ACC)	yphE	putative sugar ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base T (5/30);  new base G (25/0);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 5.49e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTC  >  NC_000913/2677891‑2678184                                                                                                                                                     |                                                                                                                                                  caGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGt                                                                                                                                                   <  1:870145/149‑1 (MQ=255)  aGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGAACGAGGCAGAGGgttt                                                                                                                                                >  1:583529/1‑151 (MQ=255)      gctTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCTGACCGAGGCAGAGGgttttc                                                                                                                                              >  1:286892/1‑147 (MQ=255)        ttttGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGGGGGAGAGGGTTTTCGccc                                                                                                                                          >  2:369814/1‑150 (MQ=255)         tttGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCa                                                                                                                                          <  1:990841/150‑1 (MQ=255)         tttGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCa                                                                                                                                          <  2:1231614/150‑1 (MQ=255)         tttGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGATAATCAATCAGGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCa                                                                                                                                          <  2:980374/150‑1 (MQ=255)               ccGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCa                                                                                                                                    <  2:468366/150‑1 (MQ=255)                   gCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCGAGGCAGAGGGTTTTCGCCACTGTCAGccc                                                                                                                                >  1:826048/1‑150 (MQ=255)                      tAGAGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCGAATGACCGCGGGGGAGGGATTTCGCCCCCCTCAGGCCttgc                                                                                                                            >  2:933872/1‑149 (MQ=255)                       aGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGGCCGAGGGCGAGGGTTTCCGCCCCCGTCCGccccttc                                                                                                                            >  2:986959/1‑146 (MQ=255)                          gTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGCCCGCGGGAGAGGGTTTTTGCCACTGTCAGCCCTTccacc                                                                                                                         >  2:437383/1‑150 (MQ=255)                           ttGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTccacc                                                                                                                         <  2:35325/149‑1 (MQ=255)                            tGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAg                                                                                                                       <  1:18612/150‑1 (MQ=255)                              aaaCAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGAc                                                                                                                     <  2:1272941/150‑1 (MQ=255)                                  aaGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAAc                                                                                                                <  1:462830/151‑1 (MQ=255)                                    gttgttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTGACCCAGGGAGGGGGTTTTCGCCCCCCTCAGCCCCTCCACCacac                                                                                                                     >  2:1140660/1‑144 (MQ=255)                                      tgttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCACTGACCCAGGCAGAGGGTTTTCGCCCCTGTTAGGCCTTCCACCCAACTTAACTc                                                                                                              >  1:1119739/1‑149 (MQ=255)                                       gttCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGAACCAGGGAGAGGTTTTTCCCCCCTCTCAGCCCCTCCCCCCCACCTAACTcc                                                                                                             >  1:217240/1‑149 (MQ=255)                                          cAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATa                                                                                                        <  2:1025505/151‑1 (MQ=255)                                            ggACTAACGTCAACGCCCAGCGCTTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATaaa                                                                                                      <  2:1240055/151‑1 (MQ=255)                                             gACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCAAAGCAGGGGGTTTTCGCCCCTTTCTTCCCTCCCCCCCGACTTCACTccctgtaaa                                                                                                      >  1:1030370/1‑143 (MQ=255)                                                tAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTCCCCGAGGCGGAGGGTTTTTGCCACCATCAGCCCCTCCACCAGAATCAACACCTTATaaa                                                                                                      >  2:550866/1‑147 (MQ=255)                                                  aCGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACAGACCGAGGGCGAGGGTTTTCGCCACAGTCAGCCCTTCCACCAGCATTAAATCCTGATaa                                                                                                       >  2:1162079/1‑144 (MQ=255)                                                      cAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGCGGGAGAGGGTTTTCGCCCCTGTCAGCCCCTCCACCCGACTTAACTCCTGATAAACcgcgcg                                                                                               >  1:1152310/1‑148 (MQ=255)                                                       aaCGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCa                                                                                             <  1:1039250/149‑1 (MQ=255)                                                         cGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCAccc                                                                                          <  1:823354/150‑1 (MQ=255)                                                         cGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCCAGGCAGAGGGTTTTCGCCCCTCTCAGCCCCTCCACCCGACTTAACTCCCGATAAACCGCGCGcccccc                                                                                         >  2:1209052/1‑151 (MQ=255)                                                            ccAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCCCTGTCAGCCCTTCCACCCGACTTAACTCCTGGTAAACCGCGCGCAccccc                                                                                        >  1:564580/1‑149 (MQ=255)                                                             cAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCCACCGAGGCCGGGGGTTTTCGCCCCCGTCAGCCCCTCCCCCCGACTTAACTCCCGGTAAACAGCGCGCccccccc                                                                                       >  2:245268/1‑148 (MQ=255)                                                             cAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCTGCCCCGGGAGAGGGTTTTCGCCCCCGTCAACCCCTCCACCCCACTTAAATCCCTATAAAACCCCCGCccccccc                                                                                       >  2:1051375/1‑148 (MQ=255)                                                             cAGCGCCTGTAAGCAACGTTGGGCATCCGGCGCAATTTGCAGGTAATCAATCATCCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCa                                                                                       <  2:376946/149‑1 (MQ=255)                                                              aGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCTCGCCGGCGGCGGGGGTTTCACCCCCCGGCCTCCCTTCCCCCCGACTTTATTCGCCATGAAACGCGCGCAccccc                                                                                        >  2:10923/1‑147 (MQ=255)                                                                  ccTGTAAGCAACGTTGGGCATCCTTCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGTCAACTGTCCGAGTCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGc                                                                                <  1:746242/151‑1 (MQ=255)                                                                        aGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACg                                                                          <  1:595706/151‑1 (MQ=255)                                                                           aaCGTTGGGCATCCTGCGCCATTTGCAGGTAATCAACCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACg                                                                          <  2:735866/148‑1 (MQ=255)                                                                                  ggCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGc                                                                <  2:136096/151‑1 (MQ=255)                                                                                   gCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCg                                                               <  2:1291445/151‑1 (MQ=255)                                                                                     aTCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTa                                                             <  1:1177858/151‑1 (MQ=255)                                                                                       ccTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACCGACCCAGGCAGAGGGTTTCCCCCCCTCTCAGCCCTTCCCCCAGACTTAAATCCTGATAAAACGCGCGCACCCCCAGTTCAGCGGCACGGCg                                                                       >  1:1313029/1‑139 (MQ=255)                                                                                              cATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCCCCGACCGCGGGCGAGGGTTTTTCCCCCCGTCAGCCCCTCCCCCAGACTTAACTCCTGATAAAACCCCCGCACCCCCCGCTCAGCGGCCCGGCGGGGCAGGGGAGCgtcgtcc                                                    >  2:525893/1‑150 (MQ=255)                                                                                               aTTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTAAACGAGGGAAAGGGTTTCCGCCACCGTCAGGCCCTCCACCAGACCTAAAACCTGATAAAACACGCGCACCCCCCGCTCCACGCGACGGCGAGCGAGCGTAGCCTCGtgcc                                                   >  2:49655/1‑148 (MQ=255)                                                                                                  tGCAGGTAATCAATCATGCCGTTGCGGCGGGGGCCCCGCCCGCGGGAGAGGGTTTTCGCCCCTGTCCGCCCCTCCCCCAGGCCTTAATCCCGATAAAACCCCCGCCCCCCCCCCTCAGCGGCACGGCGGGGCCGCGGAGGTTCGTCAcc                                                  >  2:119537/1‑149 (MQ=255)                                                                                                   gCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCtt                                                <  2:1379146/150‑1 (MQ=255)                                                                                                      ggTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAg                                            <  2:652434/151‑1 (MQ=255)                                                                                                        tAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGGTTTTCGCCCCTGTCCGCCCTTCCACCCGACTTAAATCCTGATAAACCGCGCGCCCCCCCCGTTCAGCGGGACGGCGGGGCAGCGCAGCTTCTTCACCCTcc                                              >  1:1106739/1‑147 (MQ=255)                                                                                                         aatcaatcATGCCGTTGCGGCGGGGCCACTTACCGGGGGAGAGGGTTTCCGCCACTGCCAGCCCCTCCCCCAGAATTAACTTCTCATAAACCGCCCGCCCCCCCCGTTCAGCGGCACGGCGAGTCAAACTCGGTTCGTCACCTTCCATTcg                                         >  2:1321591/1‑151 (MQ=255)                                                                                                              atcaTGCCGTTGCGGCGGGGCCCCTGACCCAGGCAGAGGGTTTCCGCCCCTGTCAGCCCCTCCCCCCGACTTAACTCCTGATAAAACCCGCGCACCCCCCGCTCAACGGCACGGCGGGGCCGCGTAGCTTTGTCCCCCTcc                                              >  1:765227/1‑141 (MQ=255)                                                                                                               tcaTGCCGTTGCGGCGGGGCCACTGACCGCGGGAGAGGGTTTTCGCCCCTGTCAACCCCTCCACCCGACTTAACTCCCGATAAAACCCGCGCACCCCCCGCTCAGCGGCACGGCGGGTCAGCGTAGCCTTGTCCCCCTCCCGTCGCGgctc                                   >  2:885984/1‑151 (MQ=255)                                                                                                                 aTGCCGTTGCGGCGGGGCCACCGGCCGGGGCAGAGGGTTTTAGCCACTGTCAGCCCTTCCCCCAGACTTAAACCCTCATAAACCCCCCCCGCCCCCAGGACACCGGGACGGCGAGACAAAGTAGGCTCGTCACCTTCCacccgcccctc                                   >  2:300494/1‑139 (MQ=255)                                                                                                                                  ccccTGACCGAGGCAGAGGTTTTCCGCCTCTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCAcc                 <  2:319988/147‑1 (MQ=255)                                                                                                                                  ccACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCg                <  1:659893/151‑1 (MQ=255)                                                                                                                                   cACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGc               <  2:610683/151‑1 (MQ=255)                                                                                                                                   cACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGc               <  1:7168/151‑1 (MQ=255)                                                                                                                                    aCTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCt              >  1:906387/1‑151 (MQ=255)                                                                                                                                      tGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCAc                  <  2:85058/145‑1 (MQ=255)                                                                                                                                       gACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATc           <  1:980935/151‑1 (MQ=255)                                                                                                                                         ccGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCg         >  1:365032/1‑151 (MQ=255)                                                                                                                                         ccGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCg         <  1:23867/151‑1 (MQ=255)                                                                                                                                            aGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGc      >  1:615565/1‑151 (MQ=255)                                                                                                                                                agagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTc  >  1:829936/1‑151 (MQ=255)                                                                                                                                                 gagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTc  <  2:490763/150‑1 (MQ=255)                                                                                                                                                 gagGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTc  <  1:58842/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CAGCTGCTTTTGCGCCGGGCTTAGCGTTGAAACAAGTTGTTCAGGACTAACGTCAACGCCCAGCGCCTGTAAGCAACGTTGGGCATCCTGCGCCATTTGCAGGTAATCAATCATGCCGTTGCGGCGGGGCCACTGACCGAGGCAGAGGTTTTCCGCCACTGTCAGCCCTTCCACCAGACTTAACTCCTGATAAACCGCGCGCACCCCCAGTTCAGCGGCACGGCGAGTCAGCGTAGCTTCGTCACCTTCCAGTCGCGTCTCGCCAATCCAGATATCACCGCTATCCGGGCGTTC  >  NC_000913/2677891‑2678184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑