Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,921,173	0	T	C	39.7%	100.6 / 16.6	63	I310V (ATT→GTT)	gudP	putative D‑glucarate transporter
Reads supporting (aligned to +/- strand):  ref base T (35/3);  new base C (0/25);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.34e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACC  >  NC_000913/2921023‑2921323                                                                                                                                                       |                                                                                                                                                       agGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAaataat                                                                                                                                                        <  2:661790/151‑1 (MQ=255) agGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAaataat                                                                                                                                                        <  1:887153/151‑1 (MQ=255)     cAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATccc                                                                                                                                                     >  1:815874/1‑150 (MQ=255)              aaaGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGcac                                                                                                                                            <  2:1096984/150‑1 (MQ=255)               aaGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCacac                                                                                                                                          >  2:895724/1‑151 (MQ=255)                aGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAcc                                                                                                                                         <  2:522956/151‑1 (MQ=255)                  ccGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAccgc                                                                                                                                       >  1:79846/1‑151 (MQ=255)                    gatgatCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAccgccg                                                                                                                                     >  2:700348/1‑151 (MQ=255)                      tgatCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGAt                                                                                                                                   >  1:138246/1‑151 (MQ=255)                        atcatcCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATa                                                                                                                                  >  2:1384899/1‑150 (MQ=255)                          cacccACCCAACGTTGACGTATTTGCAGAACCCCATCCCCATCGACAGCAACAGCCCCATTACGATCGGTTTTTTCCGCGCAATGTTCAGCGATCCCGTGCGGCGCACCAGCAAGTGGGAAATAACCCCCCCCAGCACACCGCCGATaaa                                                                                                                                <  1:1221583/147‑1 (MQ=255)                             ccACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccg                                                                                                                            >  2:279500/1‑151 (MQ=255)                               aCTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGGGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgc                                                                                                                           >  1:1037210/1‑150 (MQ=255)                                   aaCTTTGACGTAGTTGCAAACCCCCATCCCCATCGACAGCAACATCCCCATTACGATCGGTGTTTTACGCGCAATGTCCAGCGCCCCGGTGCGGCGCATCAGCCAGTCGAAAATAACCCCACCCAGCACACCGCCGATAaaaccgcaaac                                                                                                                       <  2:1309657/150‑1 (MQ=255)                                   aaCGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAaaaccgcaaacc                                                                                                                      >  2:547190/1‑151 (MQ=255)                                   aaCGTTGACGTAGTTGCAAAACACCATCCCCATCGACAGCAACATCCCCATTACGATCGGTTTTTTCCGCGAAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCCCCCAGCACACCGCCGATAaaaccgcaaacc                                                                                                                      <  1:1235166/151‑1 (MQ=255)                                       tggACGTAGTTGCAGAACCCCATCACCATCGACAGCAACATGCCAATTACGATCGGTTTTTTCCGCGCAATGTTCAGCGACCCCGTGCGGCGCACCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAaaaccgcaaaccg                                                                                                                     <  1:1036828/146‑1 (MQ=255)                                         gACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAAccgccg                                                                                                                  >  1:1350666/1‑149 (MQ=255)                                         gACGTAGTTGCAGAACACCACCCCCATCGACAGCAACATGCCCATTACGTTCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCGCCCAGCACACCGTCGATAAAACCGCAAAccgccg                                                                                                                  <  2:998400/149‑1 (MQ=255)                                             tAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgg                                                                                                            >  2:1044574/1‑151 (MQ=255)                                              aGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgg                                                                                                            >  2:1185608/1‑150 (MQ=255)                                              aGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaac                                                                                                              >  2:941149/1‑148 (MQ=255)                                                ttGCAAAACACCACCCCCATCAACACAACCATGCCCATACCGATCGGTTTTTTCCGCGCAATTTCCAGCGACCCCGGGCGGCCCATCAGCCAGTCGAAAAAAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCcggaacgg                                                                                                            <  1:1106195/148‑1 (MQ=255)                                                  gCAAAACCCCATCCCCACCGACACCAACATGCCCATTACGATCGGTTTTTTACGCGCAATGTCCATCTACCCCGTGCGGCGCAACACCAAGTGGGAAATAACCCCCCCCAGCAATCCGCCGATAAAACCGCAAACCGCCGGAACGGAAg                                                                                                         <  1:981613/149‑1 (MQ=255)                                                    aGAACCCCATCCCCACCGACAGCAACATGCCCTTTACGATGGGTTTTTTCCGCGAAATTTCCAGCGACCCCGGGCGGCCCATCAGCCAGTCGAAAAAAACCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGcc                                                                                                       <  1:357055/149‑1 (MQ=255)                                                      acccccacccccaccGACAGCAACGTCCCCTTTACGAGGGGTGTTTACCGCGAATGGTTCAGCGACCCCGGGCGGCCCACAAGCCGGTGGAAAATAACCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCcaca                                                                                                    <  1:1227812/140‑1 (MQ=255)                                                           catcaccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGCAGCCACAAAGccc                                                                                              >  2:483482/1‑151 (MQ=255)                                                           catcaccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGccc                                                                                              >  2:1300363/1‑151 (MQ=255)                                                            atcaccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCg                                                                                             >  2:1000545/1‑151 (MQ=255)                                                                          cAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGAc                                                                               >  1:177593/1‑151 (MQ=255)                                                                           aaCATCCCCTTTCCGATCGGTGTTTTACGCGCAATGTTCAGCGACCCCGTGCGGCGCATAACCCAGTCGAAAATACCCCCCCCCACCCCCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACa                                                                              <  1:923272/151‑1 (MQ=255)                                                                              aTGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATcc                                                                           >  2:133158/1‑151 (MQ=255)                                                                                 cctaTTCCGATGGGTTTTTTACGCCGAATGTTCGGCGACCCCGGGCGCCGCATCGGCCAGTGGAAAAAACCCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgc                                                                        <  1:1384974/148‑1 (MQ=255)                                                                                  ccATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgcg                                                                       >  1:695787/1‑151 (MQ=255)                                                                                          aTCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGc                                                                >  1:1306667/1‑150 (MQ=255)                                                                                          aTCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGc                                                                >  1:785046/1‑150 (MQ=255)                                                                                              gtgtTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc                                                             >  2:572396/1‑149 (MQ=255)                                                                                                 ttttACGCGAAATTTTCACCTCCCCGGGGCGGCACACCGCCCAGTCGAAAAAAACCCCCCCCCCCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGAt                                                         <  2:177587/150‑1 (MQ=255)                                                                                                      cgcgCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCg                                                   >  1:259490/1‑151 (MQ=255)                                                                                                       gcaaaaTGTTCAGCGCCCCCGTGGGGCGCACCAGCCAGGGGAAAATAACCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAAcc                                                    <  1:904487/145‑1 (MQ=255)                                                                                                           atttttCAGCGCCCCCGTGGGGCACTCCAGCCAGTCGAAAATACCCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAc                                              <  1:1149296/147‑1 (MQ=255)                                                                                                           aatttccAGCGACCCCGGGCGGCGCACCAGCCAGTCGAAAAAAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGaa                                               <  1:274527/144‑1 (MQ=255)                                                                                                           aaTGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAc                                              >  1:876514/1‑151 (MQ=255)                                                                                                             tGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAcc                                             >  1:506952/1‑150 (MQ=255)                                                                                                             tGTTCAGCGACCCCGGGCGGCGCATCACCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAcc                                             <  1:856401/150‑1 (MQ=255)                                                                                                              gTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCCCAACGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATTCCGCGGGGCCGGACCAGGTAAAACGGGAAcc                                             >  2:1329808/1‑149 (MQ=255)                                                                                                               ttcggaaaTCCCGGGCGGCGCACCACCCTGTGGTAAAAACCCCCACCAAGAACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGa                                                <  1:1185668/138‑1 (MQ=255)                                                                                                                ccagcaacccggggcggcgCAACAGCCAGTCGGAAAAAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAgg                                          <  1:280531/137‑1 (MQ=255)                                                                                                                   gCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaat                                      >  1:180315/1‑151 (MQ=255)                                                                                                                   gCGACCCCGGGCGGCGCATCACCCAGTCGAAAAAAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGta                                        <  1:1078672/149‑1 (MQ=255)                                                                                                                         ccGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATaaa                                   >  2:966939/1‑148 (MQ=255)                                                                                                                         ccGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaaga                                 >  1:1082390/1‑150 (MQ=255)                                                                                                                               gccgcATCACCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagtaag                           <  1:822012/148‑1 (MQ=255)                                                                                                                               gccccATCACCCAGTAGAAAAAAACCCCCCCAACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagtaagt                          <  1:900784/147‑1 (MQ=255)                                                                                                                                 cgcAGCAGCCTGTAGAAAAAAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagt                              <  1:1023104/145‑1 (MQ=255)                                                                                                                                    accAGCCAGTCGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCa                        <  1:327453/146‑1 (MQ=255)                                                                                                                                    aTCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAggg                     >  1:43261/1‑151 (MQ=255)                                                                                                                                      cAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCg                   >  2:203466/1‑151 (MQ=255)                                                                                                                                       aGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGt                  >  2:1273023/1‑151 (MQ=255)                                                                                                                                         ccaggggaaaaTAACCCCCCCAACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagtaa                            <  1:1002435/131‑1 (MQ=255)                                                                                                                                          cAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTg                >  1:90171/1‑150 (MQ=255)                                                                                                                                            gTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGAt              >  1:733669/1‑150 (MQ=255)                                                                                                                                              cGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATAc            >  1:514983/1‑150 (MQ=255)                                                                                                                                                 aaaaactCCCCCCCAGCCCCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGt         <  2:398814/144‑1 (MQ=255)                                                                                                                                                 aaaTATCCCCCCCCACCACGCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATAc            <  1:941181/147‑1 (MQ=255)                                                                                                                                                 aaaTAACCCCCCCCGCCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGAt              <  1:44690/145‑1 (MQ=255)                                                                                                                                                       tCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGAcc  >  2:447480/1‑151 (MQ=255)                                                                                                                                                       |                                                                                                                                                       AGGCCAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACTGACC  >  NC_000913/2921023‑2921323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑