Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,657	0	A	G	40.6%	111.0 / 16.4	69	F386S (TTC→TCC)	yhfW	phosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (12/29);  new base G (28/0);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 3.47e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCC  >  NC_000913/3508509‑3508806                                                                                                                                                     |                                                                                                                                                       aGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGaaa                                                                                                                                                      <  2:846984/151‑1 (MQ=255)     cACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAtt                                                                                                                                                  <  1:273005/151‑1 (MQ=255)        gttgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATccc                                                                                                                                               >  1:319672/1‑149 (MQ=255)         ttgttTTTAAAGCCGGAGAGATCAATGTGCAGATGCTCATGAGCGAGGGTGTAACCCGTCGGGTCAAAACTCATAGGGTGTCTCCCGCAATCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTcac                                                                                                                                               <  2:1058325/150‑1 (MQ=255)           gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTcacaca                                                                                                                                            <  1:1249598/151‑1 (MQ=255)              tttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGGGGGAAAAATtaca                                                                                                                                              >  2:36673/1‑146 (MQ=255)              tttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACg                                                                                                                                          <  2:1168443/150‑1 (MQ=255)                tAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATcaca                                                                                                                                              >  1:77109/1‑144 (MQ=255)                 aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGGGCGGGGGAAAAATtacacccagg                                                                                                                                        >  1:344345/1‑144 (MQ=255)                       ggAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGCGGGAAAATTTAAACACAGA‑AGcccccc                                                                                                                               >  1:233869/1‑150 (MQ=255)                            gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCa                                                                                                                          <  1:683841/151‑1 (MQ=255)                            gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCa                                                                                                                          <  2:625590/151‑1 (MQ=255)                                  tatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGAAAAAATCACACACCGG‑AGCCCCCACCTCAGaaa                                                                                                                      >  1:383101/1‑149 (MQ=255)                                     gCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCgtg                                                                                                                 <  2:1225324/151‑1 (MQ=255)                                      cAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGAAAAAATAAAACACGGG‑GGCCCCCCCCACAGAAAGAgtgg                                                                                                                >  2:1053363/1‑151 (MQ=255)                                      cAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGGGCGCGGGGAAATTTACAAACCGC‑AGtcccccccttagtaaggg                                                                                                                   >  1:863736/1‑135 (MQ=255)                                         aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTgc                                                                                                             <  2:1016543/151‑1 (MQ=255)                                         aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGGGCGGGAAAAATTACACCCGGT‑AACCCCCACACCAAAAAACgcgggg                                                                                                              >  1:759124/1‑148 (MQ=255)                                           gCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACAAACGGT‑AGCCCCCACATCAGAAAACGTGGGgcg                                                                                                            >  1:900635/1‑150 (MQ=255)                                              cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAAGCGGGCGGGAAAAATCACACACGGTAAGCCCCcccactgaaaggcggg                                                                                                                 >  2:416141/1‑131 (MQ=255)                                               aTGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGT‑AGCCCCCCCCACAGAAAGCGGGGGGCGcac                                                                                                         >  1:86797/1‑149 (MQ=255)                                                 gggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACAcc                                                                                                      <  1:561281/150‑1 (MQ=255)                                                  ggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGCGGGAAAAATTAAACACAGG‑AGACCCCCCCTCAAAAAGCGGGGGGGGcac                                                                                                         >  2:1330427/1‑146 (MQ=255)                                                     gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGCAAAATCACATAAGGT‑TTGCCCCACAACTGACAACGTGGAGCGCACACCgcgc                                                                                                  >  1:407578/1‑150 (MQ=255)                                                         gtgtAACCCGTCGGATCAAAACTCATATGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGA‑AGTCCCCCCAACAGAAAACGTTGTGCGCCCAACCAGc                                                                                                  >  1:300431/1‑146 (MQ=255)                                                          tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGG‑AGCCCCCCCAACAGAAAAAGTGGGGCGCACACCGAGccg                                                                                                >  1:91376/1‑145 (MQ=255)                                                           gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg                                                                                           <  1:1259890/151‑1 (MQ=255)                                                               cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTAAACACCGT‑AGCCCCCCCCTCAGAAAGCGGGGGGGGGACACCCAGCCGCGCAg                                                                                           >  2:565202/1‑147 (MQ=255)                                                                ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                                                                      <  2:946527/151‑1 (MQ=255)                                                                  gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatcat                                                                                    <  1:1383233/151‑1 (MQ=255)                                                                    cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGCGCGGGAAAAATAACACACGGG‑AACCCCCCCACCAGAAAACGTGGTGGGCACACCCAGCTGCGGAGCGATCATcc                                                                                  >  2:1015761/1‑151 (MQ=255)                                                                          aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCt                                                                            <  1:728345/151‑1 (MQ=255)                                                                              cTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTAAACACGGG‑AGCCCCCACATCAGAAAACGGGGGGGGCACACCCAGCCGCGTAGCGAACATcccct                                                                               >  2:80099/1‑142 (MQ=255)                                                                                cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGG‑AGCCCCCCCACCAGAAAGCGTGGGGGGCACCCCGAGCTGCGTTGCGATCCTCCCCTTCTGGTaa                                                                       >  1:1157552/1‑150 (MQ=255)                                                                                 aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAc                                                                     <  1:24279/151‑1 (MQ=255)                                                                                       tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGCGCGCGGGAAAAATTAAACACGGG‑AGACCCCCCATCAGAAAACGGGGGGGGCACACCCAGCCGCGTAGCGAACATCCCCTTCTGGTaaaa                                                                     >  2:454633/1‑144 (MQ=255)                                                                                        gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACt                                                              <  2:329369/151‑1 (MQ=255)                                                                                          ctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACACACCGT‑AGCCCCCCCATCAGAAAGCGGGGTGCGCACACCGACCCGCGTAGCGATCATCCCCTTCTGATAAAACAACACTgg                                                            >  2:172646/1‑151 (MQ=255)                                                                                              tGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACAACGG‑AGCCCCCCCATCAGAAAAAGGGGGGCGCACAACCAGCCGCGGAGCGGTCATCCCCTGCTGATAAACCAACACCGGcacc                                                        >  1:1135775/1‑151 (MQ=255)                                                                                                cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcacc                                                        <  1:1252278/149‑1 (MQ=255)                                                                                                  aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGGGGGAAAAATCACACACAGG‑AACCCCCACCTCAGAAAGCGGGGGGGGGACACCCAGCTCCGGAGCGGACATCCCTTTCTGATAAAAAAGCACCggggc                                                         >  2:193236/1‑143 (MQ=255)                                                                                                  aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGG‑AGCCCCCACATCAGAAAACGTGGGGGGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAACAACACTGGccccacct                                                    >  1:648995/1‑149 (MQ=255)                                                                                                     cGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATCACACACCGG‑AGCCCCCCCATCAGAAAACGGGGTGCGCCCACCGAGCTGCGTAGCGGTCAACCCTTGCTGATAAAACAGCAACGGCACCACCTc                                                   >  1:589976/1‑149 (MQ=255)                                                                                                      ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgcg                                                <  2:368911/151‑1 (MQ=255)                                                                                                       gagggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCgg                                               <  2:337047/151‑1 (MQ=255)                                                                                                         gggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGt                                             <  2:896098/151‑1 (MQ=255)                                                                                                         gggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGt                                             <  2:595547/151‑1 (MQ=255)                                                                                                           gaggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAt                                           <  2:1126737/151‑1 (MQ=255)                                                                                                           gaggaAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGGAAAAATTAAACACCGG‑AGCCCCCCCCACAAAAAGCGGGGGGGGCACACCCAGCTGCGTAGCGATCAATCCTTTCTGTTAAAACAACACTGGCACCACATCGCcgg                                              >  2:1299524/1‑148 (MQ=255)                                                                                                            aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtg                                          <  1:189115/151‑1 (MQ=255)                                                                                                            aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtg                                          <  2:91638/151‑1 (MQ=255)                                                                                                              gaaagaaaAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACAAACGGG‑AGCCCCCACCTCAGAAAGCGCGGGGCGCACACCGAGCCGCGGCGCGATCATCCCCTTCTGATAAAACAACACCGGCACCACTTCGCGGGGAtgg                                         >  1:498512/1‑150 (MQ=255)                                                                                                              gaaagaaaAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACAAACAGT‑AGCCCCCCCATCAGAAAAAGGGGGGCGCGCACCCAGCGGCGGAGCGGTGATCCCCTGCTGTTGAACCAACACCCGCACCACCTCTCgggggtg                                          >  2:82569/1‑145 (MQ=255)                                                                                                                aagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGT‑AGCCCCCACATCAGAAAACGGGGGGCGCACACCGAGCCGCGTAACGATCATCCCCTTCTGGTAAAACAACACTGGCCCCACTTCGCGGGGAtggggg                                      >  1:835555/1‑151 (MQ=255)                                                                                                                           gACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAAt                           <  1:1366784/151‑1 (MQ=255)                                                                                                                               aTTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTc                       <  1:996299/151‑1 (MQ=255)                                                                                                                                 tttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCg                      <  1:453980/150‑1 (MQ=255)                                                                                                                                  ttGTGGCGGTGGGGCGCGGGAAAAATCACACACGGT‑AGCCCCCACATCAGAAAACGGGGGGGGCACACCCAGCTGCGTAGCGATCATCCCTTTCTGATAAAACAGCACTGGCACCACTTCGCGGGGATGGTGGCTGTGACCAAAGGGCgg                     >  2:681969/1‑150 (MQ=255)                                                                                                                                    gtggcggtggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGAt                   <  1:632209/150‑1 (MQ=255)                                                                                                                                    gtggcggtggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGAt                   <  1:175057/150‑1 (MQ=255)                                                                                                                                      ggcggtgtcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg                 <  2:482602/150‑1 (MQ=255)                                                                                                                                        cggtggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg              >  2:634095/1‑151 (MQ=255)                                                                                                                                         ggtggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGc             >  1:1243350/1‑151 (MQ=255)                                                                                                                                           tggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCATTGCTGATAAACCAGCACTGGCACCACTTCGCGGGCATGGTGGCTGTGACCAATGGTCGGATCGTTGCCg           >  2:1257249/1‑151 (MQ=255)                                                                                                                                                cgaggAAAAATCCACACACGGT‑ATCCCCCACATAAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGCTCAACCAGCAATGGGACCTCTTTGCGGGCATGGGTGCTGTGATCACTAGGCGTATCCTCGCCGCGAt       >  2:481747/4‑150 (MQ=255)                                                                                                                                                 gcgGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc     >  1:1017114/1‑151 (MQ=255)                                                                                                                                                 gcgGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc     >  1:100571/1‑151 (MQ=255)                                                                                                                                                  cgGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg    >  2:548661/1‑151 (MQ=255)                                                                                                                                                   ggAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTAGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg    >  2:768027/1‑150 (MQ=255)                                                                                                                                                    gAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGcc  >  1:531197/1‑151 (MQ=255)                                                                                                                                                     aaaaaTTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGcc  >  2:815459/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                       AGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCC  >  NC_000913/3508509‑3508806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑