Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,662	0	T	A	39.7%	118.0 / 33.3	73	E384D (GAA→GAT)	yhfW	phosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base T (13/31);  new base A (29/0);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.45e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAC  >  NC_000913/3508513‑3508811                                                                                                                                                      |                                                                                                                                                       cACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAtt                                                                                                                                                       <  1:273005/151‑1 (MQ=255)    gttgttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATccc                                                                                                                                                    >  1:319672/1‑149 (MQ=255)     ttgttTTTAAAGCCGGAGAGATCAATGTGCAGATGCTCATGAGCGAGGGTGTAACCCGTCGGGTCAAAACTCATAGGGTGTCTCCCGCAATCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTcac                                                                                                                                                    <  2:1058325/150‑1 (MQ=255)       gttTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTcacaca                                                                                                                                                 <  1:1249598/151‑1 (MQ=255)          tttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGGGGGAAAAATtaca                                                                                                                                                   >  2:36673/1‑146 (MQ=255)          tttAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACg                                                                                                                                               <  2:1168443/150‑1 (MQ=255)            tAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATcaca                                                                                                                                                   >  1:77109/1‑144 (MQ=255)             aaaGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGGGCGGGGGAAAAATtacacccagg                                                                                                                                             >  1:344345/1‑144 (MQ=255)                   ggAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGCGGGAAAATTTAAACACAGA‑AGcccccc                                                                                                                                    >  1:233869/1‑150 (MQ=255)                        gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCa                                                                                                                               <  2:625590/151‑1 (MQ=255)                        gaTCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCa                                                                                                                               <  1:683841/151‑1 (MQ=255)                              tatGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGAAAAAATCACACACCGG‑AGCCCCCACCTCAGaaa                                                                                                                           >  1:383101/1‑149 (MQ=255)                                 gCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCgtg                                                                                                                      <  2:1225324/151‑1 (MQ=255)                                  cAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGAAAAAATAAAACACGGG‑GGCCCCCCCCACAGAAAGAgtgg                                                                                                                     >  2:1053363/1‑151 (MQ=255)                                  cAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGCGGGGCGCGGGGAAATTTACAAACCGC‑AGtcccccccttagtaaggg                                                                                                                        >  1:863736/1‑135 (MQ=255)                                     aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTgc                                                                                                                  <  2:1016543/151‑1 (MQ=255)                                     aTGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGGGCGGGAAAAATTACACCCGGT‑AACCCCCACACCAAAAAACgcgggg                                                                                                                   >  1:759124/1‑148 (MQ=255)                                       gCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACAAACGGT‑AGCCCCCACATCAGAAAACGTGGGgcg                                                                                                                 >  1:900635/1‑150 (MQ=255)                                          cATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAAGCGGGCGGGAAAAATCACACACGGTAAGCCCCcccactgaaaggcggg                                                                                                                      >  2:416141/1‑131 (MQ=255)                                           aTGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGT‑AGCCCCCCCCACAGAAAGCGGGGGGCGcac                                                                                                              >  1:86797/1‑149 (MQ=255)                                             gggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACAcc                                                                                                           <  1:561281/150‑1 (MQ=255)                                              ggCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGGGCGGGAAAAATTAAACACAGG‑AGACCCCCCCTCAAAAAGCGGGGGGGGcac                                                                                                              >  2:1330427/1‑146 (MQ=255)                                                 gAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGCAAAATCACATAAGGT‑TTGCCCCACAACTGACAACGTGGAGCGCACACCgcgc                                                                                                       >  1:407578/1‑150 (MQ=255)                                                     gtgtAACCCGTCGGATCAAAACTCATATGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGA‑AGTCCCCCCAACAGAAAACGTTGTGCGCCCAACCAGc                                                                                                       >  1:300431/1‑146 (MQ=255)                                                      tgtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACACGGG‑AGCCCCCCCAACAGAAAAAGTGGGGCGCACACCGAGccg                                                                                                     >  1:91376/1‑145 (MQ=255)                                                       gtAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAg                                                                                                <  1:1259890/151‑1 (MQ=255)                                                           cccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTAAACACCGT‑AGCCCCCCCCTCAGAAAGCGGGGGGGGGACACCCAGCCGCGCAg                                                                                                >  2:565202/1‑147 (MQ=255)                                                            ccGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatc                                                                                           <  2:946527/151‑1 (MQ=255)                                                              gTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGatcat                                                                                         <  1:1383233/151‑1 (MQ=255)                                                                cGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGAGGCGCGCGGGAAAAATAACACACGGG‑AACCCCCCCACCAGAAAACGTGGTGGGCACACCCAGCTGCGGAGCGATCATcc                                                                                       >  2:1015761/1‑151 (MQ=255)                                                                      aaaaCTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCt                                                                                 <  1:728345/151‑1 (MQ=255)                                                                          cTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTAAACACGGG‑AGCCCCCACATCAGAAAACGGGGGGGGCACACCCAGCCGCGTAGCGAACATcccct                                                                                    >  2:80099/1‑142 (MQ=255)                                                                            cATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGG‑AGCCCCCCCACCAGAAAGCGTGGGGGGCACCCCGAGCTGCGTTGCGATCCTCCCCTTCTGGTaa                                                                            >  1:1157552/1‑150 (MQ=255)                                                                             aTAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAc                                                                          <  1:24279/151‑1 (MQ=255)                                                                                   tgtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTTGCGCGCGGGAAAAATTAAACACGGG‑AGACCCCCCATCAGAAAACGGGGGGGGCACACCCAGCCGCGTAGCGAACATCCCCTTCTGGTaaaa                                                                          >  2:454633/1‑144 (MQ=255)                                                                                    gtCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACt                                                                   <  2:329369/151‑1 (MQ=255)                                                                                      ctcCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACACACCGT‑AGCCCCCCCATCAGAAAGCGGGGTGCGCACACCGACCCGCGTAGCGATCATCCCCTTCTGATAAAACAACACTgg                                                                 >  2:172646/1‑151 (MQ=255)                                                                                          tGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATCACACAACGG‑AGCCCCCCCATCAGAAAAAGGGGGGCGCACAACCAGCCGCGGAGCGGTCATCCCCTGCTGATAAACCAACACCGGcacc                                                             >  1:1135775/1‑151 (MQ=255)                                                                                            cAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGcacc                                                             <  1:1252278/149‑1 (MQ=255)                                                                                              aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGGGGGAAAAATCACACACAGG‑AACCCCCACCTCAGAAAGCGGGGGGGGGACACCCAGCTCCGGAGCGGACATCCCTTTCTGATAAAAAAGCACCggggc                                                              >  2:193236/1‑143 (MQ=255)                                                                                              aaCCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATTACACACGGG‑AGCCCCCACATCAGAAAACGTGGGGGGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAAACAACACTGGccccacct                                                         >  1:648995/1‑149 (MQ=255)                                                                                                 cGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAAATCACACACCGG‑AGCCCCCCCATCAGAAAACGGGGTGCGCCCACCGAGCTGCGTAGCGGTCAACCCTTGCTGATAAAACAGCAACGGCACCACCTc                                                        >  1:589976/1‑149 (MQ=255)                                                                                                  ggagggagGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTcgcg                                                     <  2:368911/151‑1 (MQ=255)                                                                                                   gagggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCgg                                                    <  2:337047/151‑1 (MQ=255)                                                                                                     gggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGt                                                  <  2:896098/151‑1 (MQ=255)                                                                                                     gggaggAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGt                                                  <  2:595547/151‑1 (MQ=255)                                                                                                       gaggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAt                                                <  2:1126737/151‑1 (MQ=255)                                                                                                       gaggaAAGAAAAGAGCGACCATTTTGTGGCGGGGGGGCGCGGGAAAAATTAAACACCGG‑AGCCCCCCCCACAAAAAGCGGGGGGGGCACACCCAGCTGCGTAGCGATCAATCCTTTCTGTTAAAACAACACTGGCACCACATCGCcgg                                                   >  2:1299524/1‑148 (MQ=255)                                                                                                        aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtg                                               <  1:189115/151‑1 (MQ=255)                                                                                                        aggaAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTAtg                                               <  2:91638/151‑1 (MQ=255)                                                                                                          gaaagaaaAGAGCGACCATTTTGTGGCGGTGGGGCGCGGGAAAAATCACAAACGGG‑AGCCCCCACCTCAGAAAGCGCGGGGCGCACACCGAGCCGCGGCGCGATCATCCCCTTCTGATAAAACAACACCGGCACCACTTCGCGGGGAtgg                                              >  1:498512/1‑150 (MQ=255)                                                                                                          gaaagaaaAGAGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACAAACAGT‑AGCCCCCCCATCAGAAAAAGGGGGGCGCGCACCCAGCGGCGGAGCGGTGATCCCCTGCTGTTGAACCAACACCCGCACCACCTCTCgggggtg                                               >  2:82569/1‑145 (MQ=255)                                                                                                            aagaaaagaGCGACCATTTTGTGGCGGTGGCGCGCGGGAAAAATTACACACGGT‑AGCCCCCACATCAGAAAACGGGGGGCGCACACCGAGCCGCGTAACGATCATCCCCTTCTGGTAAAACAACACTGGCCCCACTTCGCGGGGAtggggg                                           >  1:835555/1‑151 (MQ=255)                                                                                                                       gACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAAt                                <  1:1366784/151‑1 (MQ=255)                                                                                                                           aTTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTc                            <  1:996299/151‑1 (MQ=255)                                                                                                                             tttGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCg                           <  1:453980/150‑1 (MQ=255)                                                                                                                              ttGTGGCGGTGGGGCGCGGGAAAAATCACACACGGT‑AGCCCCCACATCAGAAAACGGGGGGGGCACACCCAGCTGCGTAGCGATCATCCCTTTCTGATAAAACAGCACTGGCACCACTTCGCGGGGATGGTGGCTGTGACCAAAGGGCgg                          >  2:681969/1‑150 (MQ=255)                                                                                                                                gtggcggtggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGAt                        <  1:632209/150‑1 (MQ=255)                                                                                                                                gtggcggtggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGAt                        <  1:175057/150‑1 (MQ=255)                                                                                                                                  ggcggtgtcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCg                      <  2:482602/150‑1 (MQ=255)                                                                                                                                    cggtggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTg                   >  2:634095/1‑151 (MQ=255)                                                                                                                                     ggtggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGc                  >  1:1243350/1‑151 (MQ=255)                                                                                                                                       tggcgCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCATTGCTGATAAACCAGCACTGGCACCACTTCGCGGGCATGGTGGCTGTGACCAATGGTCGGATCGTTGCCg                >  2:1257249/1‑151 (MQ=255)                                                                                                                                            cgaggAAAAATCCACACACGGT‑ATCCCCCACATAAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGCTCAACCAGCAATGGGACCTCTTTGCGGGCATGGGTGCTGTGATCACTAGGCGTATCCTCGCCGCGAt            >  2:481747/4‑150 (MQ=255)                                                                                                                                             gcgGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc          >  1:100571/1‑151 (MQ=255)                                                                                                                                             gcgGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATcc          >  1:1017114/1‑151 (MQ=255)                                                                                                                                              cgGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg         >  2:548661/1‑151 (MQ=255)                                                                                                                                               ggAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTAGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCg         >  2:768027/1‑150 (MQ=255)                                                                                                                                                gAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGcc       >  1:531197/1‑151 (MQ=255)                                                                                                                                                 aaaaaTTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGcc       >  2:815459/1‑150 (MQ=255)                                                                                                                                                   aaaTTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATc    <  2:218894/151‑1 (MQ=255)                                                                                                                                                    aaTTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCa   <  1:368285/151‑1 (MQ=255)                                                                                                                                                    aaTTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACGGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTTATCCGCCATCa   <  2:344337/151‑1 (MQ=255)                                                                                                                                                     aTTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAc  >  1:1330050/1‑151 (MQ=255)                                                                                                                                                      |                                                                                                                                                       CACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGGTGTCTCCTGCAAACCGGAGGGAGGAAAGAAAAGAGCGACCATTTTGTGGCGGTGGCGCGCGGAAAAATTCACACACGGT‑AGCCCCCACATCAGAAAGCGTGGTGCGCACACCGAGCTGCGTAGCGATCATCCCTTGCTGATAAACCAGCACTGGCACCACTTCGCGGGTATGGTGGCTGTGACCAATGGTCGGATCGTTGCCGTGATCCGCCATCAC  >  NC_000913/3508513‑3508811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑