Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,751,5370AC42.0% 137.7 / 13.1 81V111G (GTT→GGT) yiaTputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (37/10);  new base C (0/34);  total (37/44)
Fisher's exact test for biased strand distribution p-value = 3.82e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCTAACGTGGTC  >  NC_000913/3751388‑3751686
                                                                                                                                                       |                                                                                                                                                     
gaACGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATGCCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAc                                                                                                                                                      <  2:601970/151‑1 (MQ=255)
gaACGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAc                                                                                                                                                      <  1:920725/151‑1 (MQ=255)
   cGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAAcccc                                                                                                                                                   >  1:219598/1‑151 (MQ=255)
            cAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGc                                                                                                                                          >  1:259487/1‑151 (MQ=255)
             aGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGcc                                                                                                                                         >  2:1039904/1‑151 (MQ=255)
              ggTGTCAGCGACAGACGCCCTATCTGCATTTTGTGGAATACCGATAGCCCCCCCCCCCAGCCGTTGCTGTTACCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCg                                                                                                                                        <  1:735521/151‑1 (MQ=255)
              ggTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGCCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGcc                                                                                                                                         >  2:858700/1‑150 (MQ=255)
                 gtCAGCGACAGACGACCTATCTCCATTTTGTGGAATCCCGATACCTCCCCCACCCAGCCTTTGCTGTTATCCAGAACGTCCGCAGCTGCAGGGGCTTTCACGGTTCCCCAACGCTCGTGGTGATCCCAGGCAACCCCCGCCATAGCCGTa                                                                                                                                      <  1:1150991/150‑1 (MQ=255)
                        aCAGACGCCCTATCTGCTTTTTGTGGAATACCGATAGCCCCCCCCCCCAGCCTTTGCTGTTATCCAGAACGTCCGCAGCTGCAGGGGCTTTCACGTTTCCCCAACGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACg                                                                                                                              <  1:1151406/151‑1 (MQ=255)
                        aCAGACGCCCTATCTGCTTTTTGTGAAATCCCGATACCCCCCCCCCCCAGCCGTTGCTGTTATCCAGACCGTCCGCAGCGGCAGAGGCTTCCACGGTCCCCCACCGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTa                                                                                                                                   <  2:263431/146‑1 (MQ=255)
                        aCAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACg                                                                                                                              >  2:625193/1‑151 (MQ=255)
                         cAGACGCCCTATCTGCATTTTGTGAAATCCCAATAGCCCCCCCCCCCAGCCGTTGCTGTTACCCAAAACGTCCGCAGCTGCAGAGGCTTTCACGGTCCCCCAACGCTCGGGGGGATCCCAGGAACCCCCCGCCATAGCCGTACTATCACg                                                                                                                              <  1:714139/150‑1 (MQ=255)
                         cAGACGACCTATCTGCATTTTGTGGAATAGAGCTAACTCCCCCAACCAGCCGGTGCTGGTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGCTGCCAAACGCTCGTAGTGATACAAGGCAACCCCCGCCATAGCCGTACTTTCACGc                                                                                                                             >  1:1231185/1‑151 (MQ=255)
                          aGACGCCCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCCCCCAGCCGTTGCTGTTACCCAGAACGCCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCAc                                                                                                                               <  1:384257/148‑1 (MQ=255)
                          aGACGACCTATCTGCTTTTTGTGGAATCCCGATAGCCCCCCCCCCCAGCCGTTGCTGTTACCCAGACCGTCCGCAGCTGCAGAGGCTTTCACGTTCCCCCAACGCTCGGGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACg                                                                                                                              <  1:83602/149‑1 (MQ=255)
                           gacgacCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCtt                                                                                                                           >  2:206437/1‑151 (MQ=255)
                             cgacCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTAt                                                                                                                         >  1:410242/1‑151 (MQ=255)
                             cgacCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAACCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTAt                                                                                                                         >  1:1326079/1‑151 (MQ=255)
                                ccTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCa                                                                                                                       >  1:300441/1‑150 (MQ=255)
                                ccTACCTCCATTTTGGGGAAGACCGATGCCCCCCCCCCCCAGACGTGGCTGTTATCCAAACGGCCCGCGCCTCCAGAGGCTTTCACGGTTCCCCATCTCTGGGGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTAt                                                                                                                         <  1:1320011/148‑1 (MQ=255)
                                    tCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTg                                                                                                                  >  2:983745/1‑151 (MQ=255)
                                       gctttttGGGGAATACCGATAGCTCCCCCCCCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGt                                                                                                                 <  1:331058/146‑1 (MQ=255)
                                       gattttgGTGGAATCCCGATGGCCCCCCCCCCCAGCCGTTGCTTTTACCCAAACCGCCCCCGGCTGAAGAGGTTTCCCGGTTCCCCCACCGCTCGTGGTGACCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGtt                                                                                                                <  2:996964/147‑1 (MQ=255)
                                        cATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGc                                                                                                              >  2:1142250/1‑151 (MQ=255)
                                          ttttGTGGAATCCCGATACCCCCCCCACCCAGCCGTTGCTGTTTCCCAGACCGTCCGCAGCGGCAGAGGCTTTCACGGTCCCCCAACGCTCGTGGTGATCCCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCt                                                                                                                   <  1:970541/144‑1 (MQ=255)
                                           tttGTGGAATACCGAGACCTCCCCCCCCCAGCCGTTGCTTTTATCCAGAACTCCCGCAGCTGCAAAGGCTTCCACGGTCCCCCACCGCCCGTGGTGACCCCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCAt                                                                                                            <  1:786876/150‑1 (MQ=255)
                                                 gattacggatgcccccccccccCACCCGTTGCTGTTATCAAGACCTTCCGCAGCGGCAGGGGTTTTCCCGGTCCCCCACCCCTGGTGGTAATCCCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCAt                                                                                                       <  1:799156/135‑1 (MQ=255)
                                                  aaTACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAAt                                                                                                    >  2:1024566/1‑151 (MQ=255)
                                                   aTACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATc                                                                                                   >  2:1250633/1‑151 (MQ=255)
                                                     aCCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGt                                                                                                 >  1:1017635/1‑151 (MQ=255)
                                                      ccGATAGCCCCCCCCCCCAGCCGTTGCTGTTACCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTCCCCCAACGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGt                                                                                                 <  1:625208/150‑1 (MQ=255)
                                                       cGATAGCTCCCCCCCCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCa                                                                                               <  1:90832/151‑1 (MQ=255)
                                                        gATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCAt                                                                                              >  1:328258/1‑151 (MQ=255)
                                                        gATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCAt                                                                                              >  1:342028/1‑151 (MQ=255)
                                                        cgacaccccccccccccACCGTTGCCTGTAACCCAGACCGCCGGCAGTTGAAGAGCCTTTCACGTTCCCCCAACACTGGGGGTAATCCCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTc                                                                                                <  1:375506/143‑1 (MQ=255)
                                                         aTAGCCCCCCCCCCCAGCCGTTGCTGTTATCCAGACCGCCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCAtt                                                                                             <  2:1375580/151‑1 (MQ=255)
                                                             cccccccACCCAGCCTTTGCTGTTACCCAGAACGTCCGCAGGTGCAGAGGCTTCCCCGGTTCCCCACCGCCGGCGGTGATACCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATc                                                                                          <  1:812974/148‑1 (MQ=255)
                                                             cTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCg                                                                                         >  1:648724/1‑151 (MQ=255)
                                                               ccccccccCAGCCGTGGCTTTTACCCAGACCGTCCGCAGCTGCAGAGGCTTTCCCGTTCCCCCACCGCTCGTGGTAATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATc                                                                                          <  2:1015039/148‑1 (MQ=255)
                                                                 cccacccaGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGt                                                                                       >  2:838886/1‑149 (MQ=255)
                                                                  ccacccaGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAgg                                                                                    >  1:80055/1‑151 (MQ=255)
                                                                      ccaGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTc                                                                                 >  2:469221/1‑150 (MQ=255)
                                                                       caGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCa                                                                                >  2:792049/1‑150 (MQ=255)
                                                                        aGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCaa                                                                               >  2:790497/1‑150 (MQ=255)
                                                                             ttGCTGTTATCCAGACCTTCCGAAGCTGCAGAGGTTTTCCCGGTTCCCCACCGCTCGGGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTcc                                                                          <  1:872889/150‑1 (MQ=255)
                                                                                 cTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCg                                                                      >  1:846019/1‑151 (MQ=255)
                                                                                    ttATCCAGAACGCCCGCAGCGGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGc                                                                    <  1:119803/150‑1 (MQ=255)
                                                                                      acccAGAACGTCCGCAGCGGCAGAGGCTTTCACGGTCCCCCAACGCTCGTGGTTATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCAt                                                                  <  1:985055/148‑1 (MQ=255)
                                                                                         cAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATcc                                                                >  1:1362071/1‑149 (MQ=255)
                                                                                          aGACCGTCCGCAGCGGCGGGGGCTTCCCCGGTCCCCCACCGCTCGGGGTGATACCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATc                                                                 <  2:970759/147‑1 (MQ=255)
                                                                                            aaCGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAgg                                                             >  2:1342534/1‑149 (MQ=255)
                                                                                               gTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATAGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCg                                                        >  1:1092426/1‑151 (MQ=255)
                                                                                                 ccGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCgg                                                       >  1:117144/1‑150 (MQ=255)
                                                                                                     aGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAgg                                                  >  2:901718/1‑151 (MQ=255)
                                                                                                     aGCTGCAGAGCCTTTCACGTTCCCCCACCGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAg                                                   <  1:376614/150‑1 (MQ=255)
                                                                                                       cTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCt                                                >  2:271569/1‑151 (MQ=255)
                                                                                                           agagGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGt                                            >  2:1347429/1‑151 (MQ=255)
                                                                                                            gagGCTTTCACGGTTCCCAAACGCTCTTGGTGATCCCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTa                                               <  1:1178004/147‑1 (MQ=255)
                                                                                                              gcctcccccGGTTCCCCAACGCTCGGGGGATTGCCAGGACCCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTc                                          <  2:993504/142‑1 (MQ=255)
                                                                                                                 tttCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAttt                                      >  1:12130/1‑151 (MQ=255)
                                                                                                                 tttCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAttt                                      >  1:1316281/1‑151 (MQ=255)
                                                                                                                  ttCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAtttt                                     >  1:1092586/1‑151 (MQ=255)
                                                                                                                    cccGGTCCCCCAACGCTCGTGGTAATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAtttt                                     <  2:198165/147‑1 (MQ=255)
                                                                                                                    cACGGTCCCCCAACGCTCGTGGTGATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAttttt                                    <  2:861693/150‑1 (MQ=255)
                                                                                                                      cgtttCCCCACCGCGCGGGTTGAGCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCAttt                                      <  2:932058/143‑1 (MQ=255)
                                                                                                                       ggTTCCCCAACGCCCGGGGTGATCCCAGCCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTc                                 <  2:219591/150‑1 (MQ=255)
                                                                                                                       ggTCCCCCAACGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCa                                <  1:278100/151‑1 (MQ=255)
                                                                                                                         ttCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACt                              >  2:1045919/1‑151 (MQ=255)
                                                                                                                          tcccccAACGCTCGTGGTGATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCa                                <  1:267731/147‑1 (MQ=255)
                                                                                                                          tCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACtt                             >  1:1127992/1‑151 (MQ=255)
                                                                                                                                       gtggtgATCCCGGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATa                 <  1:858729/151‑1 (MQ=255)
                                                                                                                                       gtggtaATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCg                         <  2:607743/143‑1 (MQ=255)
                                                                                                                                       gtggtaATCCCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCg                         <  2:1054294/143‑1 (MQ=255)
                                                                                                                                         gttgATACCGGGAACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAAt                  <  2:1272652/146‑1 (MQ=255)
                                                                                                                                         ggtgATCCCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATa                 <  2:1277425/149‑1 (MQ=255)
                                                                                                                                         ggtgATACCGGACACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATaaa               <  1:910757/151‑1 (MQ=255)
                                                                                                                                         ggtgATACCAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCATCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATaaa               <  1:1213353/151‑1 (MQ=255)
                                                                                                                                             aTACCGGGAACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAAc             <  2:434347/149‑1 (MQ=255)
                                                                                                                                              tACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCt           >  2:248948/1‑150 (MQ=255)
                                                                                                                                                ccgggCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCt           <  2:1100612/145‑1 (MQ=255)
                                                                                                                                                ccAGGCACCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCTaa         <  2:670395/150‑1 (MQ=255)
                                                                                                                                                      aaCCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCTAACGTGGTc  >  2:122194/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
GAACGCCCAGCGCAGGTGTCAGCGACAGACGACCTATCTGCATTTTGTGGAATACCGATAGCTCCCCCACCCAGCCGTTGCTGTTATCCAGAACGTCCGCAGCTGCAGAGGCTTTCACGGTTCCCCAACGCTCGTGGTGATACCAGGCAACCCCCGCCATAGCCGTACTATCACGCTTATCAAGCTGTTGCATGGCATAATCGTCATTATCGGTAGGGTCAAATTCCAGCGGCATCCAGGATGCGGTCAGGCTAAGTTCATTTTTTTCACTTTGCGACAGAATAAAACCTAACGTGGTC  >  NC_000913/3751388‑3751686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: