Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT46.9% 48.5 / 45.7 65S62F (TCT→TTT) bglJbgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (7/27);  new base T (30/0);  total (38/27)
Fisher's exact test for biased strand distribution p-value = 7.46e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604198‑4604492
                                                                                                                                                   |                                                                                                                                                     
cATAGAAAAATGCGTCATGAGTAGTATCGGGATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTGTACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttt                                                                                                                                                   <  1:748277/151‑1 (MQ=255)
     aaaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTtttttcttttttt                                                                                                                                                >  2:95860/1‑147 (MQ=255)
      aaaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGCTTGCGGCGGTCATTTTTTTCTTTTTTTGc                                                                                                                                              >  1:125548/1‑151 (MQ=255)
       aaaTGCGTCATGAGTAGAATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCCAtttttttttttttttcc                                                                                                                                             >  1:1092325/1‑145 (MQ=255)
          tGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGcc                                                                                                                                             >  1:296362/1‑147 (MQ=255)
           gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgag                                                                                                                                        <  1:1386286/151‑1 (MQ=255)
           gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga                                                                                                                                         <  2:1109993/150‑1 (MQ=255)
             gTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGaa                                                                                                                                      <  1:1016099/151‑1 (MQ=255)
               cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttttttccctgcgga                                                                                                                                      >  1:725393/1‑137 (MQ=255)
               cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTAGaa                                                                                                                                      >  2:369140/1‑149 (MQ=255)
                aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAg                                                                                                                                     <  1:760390/149‑1 (MQ=255)
                aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTATTCCATgatgagcg                                                                                                                                   >  1:621780/1‑145 (MQ=255)
                 tGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTTCCATGAGaa                                                                                                                                      >  2:560215/1‑147 (MQ=255)
                  gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATTAGGAggggg                                                                                                                                 >  1:929631/1‑147 (MQ=255)
                  gAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGa                                                                                                                                  <  2:1360372/150‑1 (MQ=255)
                        taTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTTCCATGAGGAGTGAGGGCag                                                                                                                            >  1:1059568/1‑150 (MQ=255)
                           cGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTTGGAAGTGGGGGCcgag                                                                                                                          >  2:840293/1‑149 (MQ=255)
                            ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAgg                                                                                                                       >  1:476123/1‑151 (MQ=255)
                               aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCGGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCGTTTTTTTTTTTTTTGCCATGAGAAGGGGGGGCAGAGAggg                                                                                                                      >  2:1303577/1‑149 (MQ=255)
                               aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTACTTTTTTTTTTTTCTTCCATGAGAAGTGAGCCCAGAGAggg                                                                                                                      >  1:7905/1‑149 (MQ=255)
                                  gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCAGAGggggggttt                                                                                                                 >  2:449405/1‑146 (MQ=255)
                                  gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTAt                                                                                                                <  1:1123726/151‑1 (MQ=255)
                                    gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCCGTTTTTTTTTTTTTTGGCGTTTGAAGGGAGGGCCGAGggggggt                                                                                                                   >  2:614902/1‑144 (MQ=255)
                                     agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCCTGAGAAGGGAGGGCAGAGGGGGATTATCtt                                                                                                             >  1:282437/1‑151 (MQ=255)
                                              aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGTTCTGTTTTTAATAATATACCATGGGAAGGGAGGGGGGAGAGGGAGTATCTTGCcttact                                                                                                     >  1:893352/1‑150 (MQ=255)
                                                aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGaa                                                                                                  <  2:587972/151‑1 (MQ=255)
                                                 aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGAGTATCTTGCCTGACTGaa                                                                                                  >  2:1156315/1‑150 (MQ=255)
                                                    tttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTg                                                                                               <  1:1315806/150‑1 (MQ=255)
                                                     ttGCGGGTAACCCTTATAAGCTCCATGCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGTATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTgg                                                                                              <  1:1374642/150‑1 (MQ=255)
                                                         gggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGGGGGCAGAGGGGGGTTATTTTTCcttac                                                                                                      >  2:512481/1‑138 (MQ=255)
                                                          ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTa                                                                                        <  2:784056/151‑1 (MQ=255)
                                                              aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCCTGAGAAGAGAGCGCAGCGAGGGGGTTTCTTGCctgactggattcggggtt                                                                                         >  2:28760/1‑135 (MQ=255)
                                                              aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTTGGAAAGGAGAGCCGAGAGGGATTATTTTTGCCCGCTGAAGTGGGGATTTAggt                                                                                    >  1:942388/1‑149 (MQ=255)
                                                               cccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGttt                                                                                   <  1:143084/151‑1 (MQ=255)
                                                                  ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGGAGTGGGGGCAGAGAGGGATTTTTTTGCCTGACTGAACTGGGGATTTAGgtttc                                                                                 >  1:1277119/1‑148 (MQ=255)
                                                                   tataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTcc                                                                                 <  2:1064009/149‑1 (MQ=255)
                                                                         cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGGAGTGTGCGCAGAGAGGGGGTATTTTTTCTTATTCAACTTGGGATTAAGTTTCTTCGTAccc                                                                         >  2:31904/1‑151 (MQ=255)
                                                                         cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGGGATTTAGTTTCCGCGTAccc                                                                         >  1:930700/1‑151 (MQ=255)
                                                                         cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCCTTTTTTTTTTTTTTTTCAAGAGGAGTGAGGGGAGGGAGGGGTTTTTTTTCCTTACTCCAATGGCCGTTAAGTTTCCCCGTAcc                                                                          >  2:948153/1‑150 (MQ=255)
                                                                           ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                                                                         <  2:1164161/149‑1 (MQ=255)
                                                                           ccATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg                                                                       <  1:716800/151‑1 (MQ=255)
                                                                            cATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAGGGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGTTTTCGCGGACCCgg                                                                       >  1:394293/1‑150 (MQ=255)
                                                                              tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGAGAGGGCAGGGGGGGGGTTTTTTTCCTGACTGAACTGGCGGTTAAGTTTTCCCGTAACCGGCg                                                                     >  2:936600/1‑150 (MQ=255)
                                                                                 cTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGGCATTAGAAGTGGGGGCAGAGGGGGGTTATCTTGCCTGAATGAACTAGCGGTTAAGGTTCCGCGTACCCGGCGgtt                                                                  >  1:763663/1‑150 (MQ=255)
                                                                                   ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAg                                                                <  2:466723/150‑1 (MQ=255)
                                                                                      ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTGTCTGACTGAACTGGCGGTTTAGTTTTCGCGGACCCCGCGTTTTGtttt                                                            >  2:1354601/1‑149 (MQ=255)
                                                                                       cAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                           <  1:613185/151‑1 (MQ=255)
                                                                                          tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc                                                         <  2:273007/150‑1 (MQ=255)
                                                                                             ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTTTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCCGAGAGGGGTTATCTTTCCTGAATGAACTTGGGGTTAAGTTTTCCCGTAACCCGCGCTTTGGTTTTGCgg                                                       >  1:654615/1‑149 (MQ=255)
                                                                                             ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGga                                                      <  1:186067/150‑1 (MQ=255)
                                                                                             ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCAATTTTTTTTTTTTTGGCATGGGAAGTGAGGGCCGGGGGGGGTTTTTTTTGCTGACTGAAATGGCGATTTAGTTTTCGCGTACCCGGCGGTTTGTTTTTGCgg                                                       >  2:5605/1‑149 (MQ=255)
                                                                                                gTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                                   <  2:621765/150‑1 (MQ=255)
                                                                                                         ggATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGaa                                         <  2:597189/151‑1 (MQ=255)
                                                                                                                aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGGTTAAGTTTCCGCGTACCCGGCGTTTTGTTTTTGCGGGTGATGATATTTAAGCTCgg                                   >  1:180377/1‑150 (MQ=255)
                                                                                                                aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCGGCGTTTTGTTTTTGCGGGTGGTGGTATTTAAACTCgg                                   >  1:566392/1‑150 (MQ=255)
                                                                                                                aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGc                                  <  2:1311294/151‑1 (MQ=255)
                                                                                                                  gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCt                                 <  2:1033370/150‑1 (MQ=255)
                                                                                                                    cgcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                               <  2:48032/150‑1 (MQ=255)
                                                                                                                     gcgGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGAtt                             <  1:1247989/151‑1 (MQ=255)
                                                                                                                      cgGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGTGAAGTGGGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGTTTTCCCGTACCCGGCGTTTTGTTTTTTCGGGTGATGATATTGAAGCTCGGCTGttt                             >  1:950280/1‑150 (MQ=255)
                                                                                                                       ggATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATTGGAAGTGAGCGCAGAGAGGGGTTTTTTTTCCTTACTGAACTGGCGGTTAAGTTTTCGCGGAACCGGCGTTTTGTTTTTTCGGGTGATGATATTTAAGGCCGGCTgggt                             >  2:142122/1‑146 (MQ=255)
                                                                                                                             cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGtt                         <  1:583621/147‑1 (MQ=255)
                                                                                                                              gTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGGGAAGGGAGCGCCGGGAGGGGTTATTTTTTCTGGCTGCAATTGCGATTAAGGTTCCGCGGACCCGGCGGTTTGTTATTGCGGGTGGTGATATTGAAAGCCGGGGGGTTGGTTCAtt                     >  2:98539/1‑150 (MQ=255)
                                                                                                                                     gcggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt             >  2:334714/1‑151 (MQ=255)
                                                                                                                                      cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc            >  2:354876/1‑151 (MQ=255)
                                                                                                                                      cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATc            >  1:119400/1‑151 (MQ=255)
                                                                                                                                         tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc          >  1:102345/1‑150 (MQ=255)
                                                                                                                                         tCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTGCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc          <  2:338398/150‑1 (MQ=255)
                                                                                                                                           aTTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGc       <  2:1177367/151‑1 (MQ=255)
                                                                                                                                              tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt      <  2:134903/150‑1 (MQ=255)
                                                                                                                                               ttttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTgg    >  1:1166635/1‑151 (MQ=255)
                                                                                                                                                tttctttttctGCCATGAGAAGAGAGCGCAGCGAGGGGTTATTTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGCTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAACGCAgg    >  2:1068860/1‑150 (MQ=255)
                                                                                                                                                  tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc  <  1:215273/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAC  >  NC_000913/4604198‑4604492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: