Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC46.8% 116.2 / 14.8 79T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (34/8);  new base C (0/37);  total (34/45)
Fisher's exact test for biased strand distribution p-value = 4.77e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGG  >  NC_000913/1376358‑1376655
                                                                                                                                                         |                                                                                                                                                      
ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAaca                                                                                                                                                        <  1:820585/148‑1 (MQ=255)
ttACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAccc                                                                                                                                                     >  2:1248837/1‑151 (MQ=255)
  aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCt                                                                                                                                                    <  1:1097878/150‑1 (MQ=255)
  aCCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTc                                                                                                                                                   >  2:714018/1‑151 (MQ=255)
     gccgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGcc                                                                                                                                                >  2:144757/1‑151 (MQ=255)
       cgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGa                                                                                                                                              <  2:1162430/151‑1 (MQ=255)
        gATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGAt                                                                                                                                             <  2:53826/151‑1 (MQ=255)
         aTGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATg                                                                                                                                            <  2:12449/151‑1 (MQ=255)
           gACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGcc                                                                                                                                          >  2:1288667/1‑151 (MQ=255)
                cgccgcGTAGGCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCccgc                                                                                                                                       <  1:666674/149‑1 (MQ=255)
                cgccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCccgc                                                                                                                                       <  1:1283639/149‑1 (MQ=255)
                 gccgcGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGt                                                                                                                                    >  1:1084171/1‑151 (MQ=255)
                          ccTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtcgtc                                                                                                                           >  2:118780/1‑151 (MQ=255)
                             ggccGGCGACCACAAAATGGGGAGTGTTTCCCGGCGCGTGCTGAAAGGGGTCGCCGCGCGTCGGGGACCGGGGGTGTATCCCGACCCTTTAACCCTTTATCAGAATAATATGATCAACCCCCGCGCCGATGCCCGCCGTTACAt                                                                                                                               <  1:52836/141‑1 (MQ=255)
                             ggACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGa                                                                                                                         >  1:636302/1‑150 (MQ=255)
                              gACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCAGTTACATCGTCGaaa                                                                                                                       <  2:1142693/151‑1 (MQ=255)
                               aCGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGaaa                                                                                                                       >  1:714955/1‑150 (MQ=255)
                                 ggCGACCGAAAAGTGGGGGGTGATTCCCTGCGCGTCCGGAAACAGGTCGCCGCGCGTCCGGGAACTGGGTTGTACCTCGACCCTTTAAACCGCTATCAGGATCATATGACAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGaaa                                                                                                                       <  2:1132804/148‑1 (MQ=255)
                                     cccGAAAAATGGTGGGTATTCCCCGCCGTGAATTGGAACAGGTCGCTGCGGGTCCGGAAACCGGGGGTTATCTGCAACCATAAACCCACTAACGGTATAATATTATAACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCt                                                                                                                <  2:92386/150‑1 (MQ=255)
                                     aCCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCt                                                                                                                >  2:426800/1‑151 (MQ=255)
                                      ccGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                               >  1:1091838/1‑151 (MQ=255)
                                      ccGAAAAAGGGGGAGTGATCCCCGGCTCGTCCGGAAAGGGGTCGCCGGGCGAACCGGAACGGTGTTTTATCCCGACCCGTAAACCCGCTAAAAGGATAAAATAATAACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                               <  1:44846/151‑1 (MQ=255)
                                        gCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTa                                                                                                              >  1:920353/1‑150 (MQ=255)
                                          aaaaTGGTGGGTGATTCCCGGCGCGTACGGGAACGGGGCGCCGCGGGTCCCGAACCGGTGGTGTATCTCGACCCTTTAACCCGCTATAAGGATCATATAATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                           <  2:823925/151‑1 (MQ=255)
                                          aaaaGGGTGAGTCATCCCCCGGGCGTCCGGGAACAGGCCCCCGCCCGTCCCGAAACGGTGTTTTCTCTCCACCCTTTAACCCCCTATAAGAATCATATATACAACCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                           <  2:1189007/151‑1 (MQ=255)
                                           aaaTGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                           >  1:272851/1‑150 (MQ=255)
                                              ggttgattgctcccgtGCGCTTACTGAAACGGGCCACCCCGCGCCCGGAACCGGGGGTGTATCGCTAGCGTTTAACCCGTTGTAGGGATCATAGAATAACCCCCCGCGCAGTTCGCCGCCGTTACATCGTCGAAAACGATCTTAAACATg                                                                                                        <  1:140119/135‑1 (MQ=255)
                                               ggTGAGTGATCCCCTCGGCTTATGGGAAAGGGTGGCGGCGCGACCGGGACCCGTGGTGTTTCCCGAACCTTAACCCCGCAATCAGGATAATATGAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACa                                                                                                          <  1:440073/147‑1 (MQ=255)
                                                gtgagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                     >  1:351736/1‑151 (MQ=255)
                                                 tgagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACAt                                                                                                         >  1:526580/1‑146 (MQ=255)
                                                    gtgttccccTGCGCGTCCGGAAACAGGCCCCCGCGCGTACCGAACCTGTGGTTTATCTCAACCCTTAAACCCGATATAAGAATAATAAGACCACCCCCCCCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGt                                                                                                       <  1:1195670/139‑1 (MQ=255)
                                                     tgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAtt                                                                                                >  2:214873/1‑151 (MQ=255)
                                                      tatccccTGCGCTTACGGAAACGGGCCGCGGCGCGACCCGGACCTGTGGTGTTTCCCAACCCTTAAACCCTTTACCAGAATCATATACCCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACa                                                                                                    <  1:50785/142‑1 (MQ=255)
                                                      gttccccTGCGCGTACTGGAACAGGTCGCCGCGCGTACGGGAACCGTGGTGTTTCCCAACCCGTAAACCCTCTATAAGAATCATATGAACACCCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                     <  2:1229005/141‑1 (MQ=255)
                                                      gaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAAc                                                                                                           >  2:293830/1‑139 (MQ=255)
                                                      catccccTGCGCGTCGTGGACCAGGTCCCCGCGCGTCCCGGACCGGTGTTTTATCCCAAACCGTAAACCCGCGTTAAGGATCATTTAACAACCCCCCCCGCCGTGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTa                                                                                               <  2:776050/147‑1 (MQ=255)
                                                        ttccttccGCTTACTGGACCAGGTCGCGGCGCTTCCCGAACCCTTGTTGTACCTCGACCCGTTAACCCGTTTCCATTAAAATATCTTAACCCCCCGCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAg                                                                                                   <  2:1064861/145‑1 (MQ=255)
                                                        ttCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                             >  2:116444/1‑151 (MQ=255)
                                                          cccTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCgg                                                                                           >  1:1065982/1‑151 (MQ=255)
                                                          cccTGCGCGTACGGAAACGGGTCGCCGCGCGTACCGGACCGGTGGTGTATCTCAACCCTTAAACCCGCTATCAGAATAATATGACCAACCCCCGCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTa                                                                                               <  2:317432/147‑1 (MQ=255)
                                                          cccTGCGCGTACGGAAACAGGTCCCCGCGCGTCCGGAACCGGTGGTTTACCCCGACCCTTAAACCCGTTAGCAGGATAATATAACAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTAt                                                                                              <  2:558314/148‑1 (MQ=255)
                                                          cccGGCGCGTCCGGGAACGGGTCCCCGCGGTTACCGGAACGGTGTTTTATCCCAACCCTTAAACCCCCTATCAGAATAATATGACCACCCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGAt                                                                                                 <  1:1024480/145‑1 (MQ=255)
                                                           cccggcttttacgtgAACGGGTCCCCCCGCGTACCGAAACCGTGGTGTATCACCATCGGTAAAACCATAAAAAGTATCATATGTTCAACCCCCCCGCCCATACCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                             <  2:579586/135‑1 (MQ=255)
                                                                cgcttatgggaaggggTCGCCGCGGGTCCCGGACGGGGGTTGTATCTCAACCCGTAAACCCCTTATGAGGATCATAGAACAACCACCCTCACCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAt                                                                                       <  1:407418/136‑1 (MQ=255)
                                                                 gcgTACTGGAAGAGGTCGCCGCGCGTACCGAACCGGGGTTGTATCTCAAACCGTAAACCCGTTATCAGGATCATATGATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGa                                                                                        <  2:280204/148‑1 (MQ=255)
                                                                 gcgTACGGAAAGAGGTCGCCGCGCGTCCCGACCCGGTGGTGTATCTCAACCTTTTAACCCTCTATCAGGATCATATAATAAACCCCCCCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAtt                                                                                      <  1:377038/150‑1 (MQ=255)
                                                                        ggAACGGGTCCCCGCGCCTCCCGGAACCGTGGTGTATCTCGAACCTTTAAACCGCTATCAGGAACATATAAAAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTAt                                                                                 <  2:979216/148‑1 (MQ=255)
                                                                         gAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                              >  2:871284/1‑150 (MQ=255)
                                                                          aaCAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACAt                                                                            >  1:1004869/1‑151 (MQ=255)
                                                                           acggttCGCCGCGCGTCCGGGAACCGTGGTTTATCTCAACCCTTTAACCCGCTATCAGGATCATATAACCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                           <  2:1123040/146‑1 (MQ=255)
                                                                           accggTCCCCGCGCCCCCCGGACCCGTGGTATACCGCGACCTGTAAAACCGGTATCAGTATATTATTACAACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTAt                                                                                 <  2:1011094/142‑1 (MQ=255)
                                                                            cAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                           >  2:988302/1‑150 (MQ=255)
                                                                              ggcccccgcGCGACCGGAACCGGTGTTGTATCCCAACCCTTAAACCCCCTATAAGAATAAAATAACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGaa                                                                         <  1:377217/145‑1 (MQ=255)
                                                                              ggTCCCCGCGGGTCCCGAACCCGGGGTTTATCCCGACCCGTAAAACGGCTATAAGAATAATATGATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATca                                                                               <  2:1062077/144‑1 (MQ=255)
                                                                                 ccccgcGCGTCCCGAACCCGTGGTTTACCCCGACCCGTTAAACCGCTATCAGGATCATATAATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAat                                                                        <  2:125085/146‑1 (MQ=255)
                                                                                    cgcgtgTCCCGAAACGGAGGTGTTTCTCAACCCGTAAACCCGTTTTCAGGAATATATAACCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata                                                                       <  2:1272660/146‑1 (MQ=255)
                                                                                    ccgcgctcccGGGACCGGTGGTGTATCCCGACCCGTAAACCCGCTATAAGAATAATATAACCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAatat                                                                      <  1:636625/140‑1 (MQ=255)
                                                                                      cgcgTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                                   >  2:276672/1‑148 (MQ=255)
                                                                                       gcgTACCGGACCCGTGGTGTATCTCGAACCTTTAACCCGCTATCAGGATCATATGAACAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAatat                                                                      <  2:946945/144‑1 (MQ=255)
                                                                                          tACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                                >  2:1125137/1‑147 (MQ=255)
                                                                                          gtccgggaccgggggtgTATCTCAACCCGTAAACCCGCTATCAGGATAATATGACCAACCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                               <  1:1187822/136‑1 (MQ=255)
                                                                                           cccGGACCCGGGGTGTATCGCGACCCTTAAACCCGCTATCAGGATCATATGACCAACCCCCTCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATa                                                           <  2:110383/150‑1 (MQ=255)
                                                                                             cggtaccggtgttgTATCCCGACCCGTTAACCCGCTACCAGGATAATATGAGCACCCCCCGCCCGGGTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaa                                                                  <  1:1048512/135‑1 (MQ=255)
                                                                                                 cccGTGTTTTTTCTCGAACCGTTAACCCTAAATCAGAATAATAATACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                                     <  2:250485/150‑1 (MQ=255)
                                                                                                  ccGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgc                                                    >  2:252232/1‑151 (MQ=255)
                                                                                                     gtgttgTATCCCAACCCTTAAACCCCTTATCAGTATCATATAATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcg                                                   <  2:1188345/150‑1 (MQ=255)
                                                                                                      tggtgTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCCCATGAATATCGAAGAAGATAACCTGgcg                                                   >  2:759632/1‑149 (MQ=255)
                                                                                                        gggttATCTCAACCCTTTAACCCGCTACCGGGTTCAAATGAACACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                                     <  2:203256/142‑1 (MQ=255)
                                                                                                            tATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgcgc                                            >  2:413379/1‑151 (MQ=255)
                                                                                                             aTCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCt                                           >  1:1088636/1‑151 (MQ=255)
                                                                                                             aTCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCt                                           >  1:54385/1‑151 (MQ=255)
                                                                                                               ctcGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGc                                         >  1:1017584/1‑151 (MQ=255)
                                                                                                               cccGACCCGTAAACCCGTTATCAGAATCATAGGACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                                          <  1:1265384/148‑1 (MQ=255)
                                                                                                                     ccGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGAc                                    >  1:361262/1‑150 (MQ=255)
                                                                                                                        ttAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAAcc                                >  1:81254/1‑151 (MQ=255)
                                                                                                                          aaaCCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                              >  2:352349/1‑151 (MQ=255)
                                                                                                                                gCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                         >  1:1166059/1‑151 (MQ=255)
                                                                                                                                cgttatcgagcacATATAATCACCCCCCTCGCAAAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAcc                          <  2:651958/139‑1 (MQ=255)
                                                                                                                                 gcaaacAGGATAATATGACAACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                         <  2:228680/146‑1 (MQ=255)
                                                                                                                                                tGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCCTGACAACCGCGACCTGCTCGGTCATGTGc           >  2:744288/1‑150 (MQ=255)
                                                                                                                                                        cacCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCg   >  1:59365/1‑151 (MQ=255)
                                                                                                                                                         acCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCgg  >  1:1170722/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                      
TTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATTGCGG  >  NC_000913/1376358‑1376655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: