Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,455	0	G	T	45.5%	47.1 / 22.0	44	intergenic (+853/+170)	yhaC/garK	pentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (3/21);  new base T (20/0);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.02e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑TCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTAT  >  NC_000913/3270306‑3270604                                                                                                                                                      |                                                                                                                                                        ttCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTg                                                                                                                                                         <  1:558009/150‑1 (MQ=255)  tCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTct                                                                                                                                                       >  2:470017/1‑151 (MQ=255)   cAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTct                                                                                                                                                       >  2:613435/1‑150 (MQ=255)   cAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGctc                                                                                                                                                      <  2:163202/151‑1 (MQ=255)        cGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGt                                                                                                                                                 >  2:387619/1‑151 (MQ=255)         gggCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTTTCTGtt                                                                                                                                                >  1:566091/1‑151 (MQ=255)         gggCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGtt                                                                                                                                                <  2:650035/151‑1 (MQ=255)              gTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACt                                                                                                                                           <  1:929460/151‑1 (MQ=255)                aCCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTTTTGccc                                                                                                                                            >  1:284268/1‑146 (MQ=255)                 ccTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTCTTGCACt                                                                                                                                           >  1:1172395/1‑148 (MQ=255)                 ccTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGt                                                                                                                                        <  2:819343/151‑1 (MQ=255)                    tATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTc                                                                                                                                       <  2:382751/149‑1 (MQ=255)                     aTGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑g                                                                                                                                   <  2:1093712/151‑1 (MQ=255)                          ccccTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGttt                                                                                                                              <  1:130617/151‑1 (MQ=255)                                tttGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTTTCTTTTTCCCTTGTCGT‑GGGGTTcccccc                                                                                                                        >  2:156279/1‑151 (MQ=255)                                 ttGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTcccccc                                                                                                                        <  2:342996/150‑1 (MQ=255)                                 ttGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGGTTTGTCTTTGTTTCACCGGTGGT‑GGGGTTtcccccc                                                                                                                       >  1:263885/1‑151 (MQ=255)                                   ggCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAg                                                                                                                     <  2:69307/151‑1 (MQ=255)                                        tGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGt                                                                                                                 <  1:1033488/150‑1 (MQ=255)                                        tGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGt                                                                                                                 <  2:226697/150‑1 (MQ=255)                                         gCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCCCTGGGCGG‑GGGGTTTCCCCCCCCGCGttt                                                                                                               >  2:83063/1‑150 (MQ=255)                                            ccGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCt                                                                                                            <  2:1290128/151‑1 (MQ=255)                                                  tgAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGTGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCAcc                                                                                                      >  1:649997/2‑151 (MQ=255)                                                         aCCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGccc                                                                                                <  2:376819/150‑1 (MQ=255)                                                              gCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATgg                                                                                           <  1:1124801/150‑1 (MQ=255)                                                                       tGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTCCTTCACTCTGCGG‑GGGGTTCCCCCCCCGGCGGTACCCCGCACCCCGCCCCATTGGGCCCCGAc                                                                                  >  1:1247612/1‑150 (MQ=255)                                                                                      ggTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTGGTCGT‑GGGGTTTCCCCCCCCGCGGTTCCTGGCACCCTGCCCCATTGAGCCCGGGCTTTCCTcccc‑cccccc                                                                 >  2:991269/1‑151 (MQ=255)                                                                                             tctTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGTCCATCGGCGGTTTTCTCCCTGTTTCACCGGCCGT‑TGGCCTCCCCCCCAGACGTTGCCTTCCCCCCCGCCCCATTGAGCCCGCACTTTCCTCCCC‑TCCTccccg                                                              >  1:174264/1‑145 (MQ=255)                                                                                             tctTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCCCTTTTTTACCGGGCGT‑GGGGTTTCCCCCCCCGCCCTCCCCGGCACCCCCCCCCCTGGGGCCCCGGCTTTTCccccc‑ccccccccccccc                                                          >  1:830866/1‑145 (MQ=255)                                                                                                tACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCACTTGGCGT‑GGGGTTCCCCCCCCGGCGTTATCCCGCCCCCCCCCCCCTGGGGGCCCGGCCTTCCTCCCC‑CCCCCCCCTCcccccc                                                       >  1:453818/1‑151 (MQ=255)                                                                                                   cGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTGGGCCC‑GGGGTTCCCCCCCACGCGGTATCTCGCCCCCCGCCCCATTGAGCCCCGCCCTTTCTCCCC‑CCCCCCCCTCTcccccc                                                      >  2:395592/1‑148 (MQ=255)                                                                                                   cGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑TCCGCCCGTCTCCCCCGaa                                                    <  1:672437/151‑1 (MQ=255)                                                                                                      aCCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCCCTGGGCGG‑GGGGTTTCCCCCCCCGCGGTTCCCGGCACCCCGCCCCCTTGGGCCCCGGCTTTTCTCCCC‑CCCCCCCCCCTccccc                                                       >  2:498957/1‑145 (MQ=255)                                                                                                           ttCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTCTTGCACCTGGCGTGGGGTTTCCCCCCCCGG‑GGTACCTCGCCCCCCGCCCCCTGGGGCCCCGCCCTTCCTCCCC‑CCCCCCCCTCcccccccccgagg                                                >  1:1075258/1‑140 (MQ=255)                                                                                                            tCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGGACCGGGCGC‑GGGGTTTCCCCCCCGGCGTTTCCCGGCCCCCCGCCCCTCGGAGCCCGGGCCTTTCTCCCC‑CCCCCCCCTCcccccccccgagg                                                >  1:349132/1‑139 (MQ=255)                                                                                                             cACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑TCCGCCCGTCTCCCCCGAAGAGgacgacg                                          <  1:957315/151‑1 (MQ=255)                                                                                                             cACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑TCCGCCCGTCTCCCCCGAAGAGgacgacg                                          <  2:360870/151‑1 (MQ=255)                                                                                                               cccTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑GCCGCCCGTCTCCCCCGAAGAGgacgacgac                                        <  1:130412/151‑1 (MQ=255)                                                                                                                 cTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGATTTCGTTCTTTTGCTTCCGCCGT‑GGGGTTCCCCCCCCCGCGGTTCCCGGCCCCCCCGCCTCTGGACCCCCGGCGTTTCTCCCCTTCCCCCCGcccccccccaggggcag                                              >  2:983636/1‑135 (MQ=255)                                                                                                                     ccTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCACTGGTCGG‑GGGGTTTCCCCCCCCGCGTTTCCCGGCACCCCGCCCCACTGAGCCCCGGCCTTCCTCCCC‑CCCCCCCCCCCCCCCCCCAGAgg                                                >  1:1063571/1‑137 (MQ=255)                                                                                                                       tGATCCCGCTTGCGCGGGCCATCGGCGGGTTTTTCTCTGTTGCGCACGGCGT‑GGGGTTCCCCCCCCCGCGGTACCTTGCCCCCCGCCCCCCGGGGGCCCGGCCTTCCCCCCC‑CCCCCCCCCCTccccc                                                       >  1:768190/1‑128 (MQ=255)                                                                                                                           cccGCTTGCGCGGGCCATCGGCGGGTTTCTCTCTGTTGCACTGGTCGT‑GGGGTTTCCCCCCCCGCGTTACCTGGCACCCCCCCCCATTGAGCCCCGGCTTTTCTCCCC‑CCCCCCCCTCCCCCCCCACGAGGACGACGACGAAacggcggc                             >  2:652764/1‑148 (MQ=255)                                                                                                                                 tGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑TCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCt                        <  1:1337396/149‑1 (MQ=255)                                                                                                                                             cggcggTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑TCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGgc           <  2:263889/150‑1 (MQ=255)                                                                                                                                                    ttGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑TCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGt    <  2:1029077/150‑1 (MQ=255)                                                                                                                                                      gCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑TCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGtat  <  2:349139/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                        TTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGT‑GGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCC‑TCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGTAT  >  NC_000913/3270306‑3270604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑