Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,483,268 | 0 | T | A | 47.3% | 47.5 / 34.1 | 56 | L24F (TTA→TTT) | emrK | multidrug resistance efflux pump membrane fusion protein |
Reads supporting (aligned to +/- strand): ref base T (17/12); new base A (0/26); total (17/39) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 8.06e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG > NC_000913/2483128‑2483416 | ttATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTa < 1:757717/148‑1 (MQ=255) tATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCg < 1:999430/150‑1 (MQ=255) tGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGcc > 1:1193091/1‑150 (MQ=255) gATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCa < 1:996648/150‑1 (MQ=255) ttAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGGCCAATAGGCATAGGCACCTCAAAACGCAATAAATAAAA‑CTACCGCCAAt > 2:677525/1‑150 (MQ=255) gTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACAAATAGGCATAGGCACCTGAAAACGCAATAAAAAAAA‑CTACCGCCAATAAAGAAAAGt < 2:925093/151‑1 (MQ=255) ccTGAGTTTTGTGCAGAATTTGACTCTGCTTTCCCGTTAAAATAGTCGTCATCTGTACGAATCTTTTCTTCAAATTCCATGGCCAAATAGGCATAGCCCCCTAAAAACGAAAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTttcttc < 2:526867/151‑1 (MQ=255) ccTGAGATTTGTGCAAAAATTGGATCTACATCCCCCGAGAAATAGGCGTCATCTGTACTAATCATCTTTTCAAATCCCATTCACCAATAGACATAGCCACCTAAAAACGCAAAAAAAAAAC‑CTACCGCCAATAAAGAAAAGTATTttctt < 1:545578/150‑1 (MQ=255) gagaCTTGTGAAAAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCTTGTCTTCTAATTCCATTGACCAATAGGAAAAGGCACCTAAAAACGAAAAAAATAAAC‑CTACCGCCAATAAAGAAAAGTATTTtcttct < 2:51894/149‑1 (MQ=255) ttgtaaaaaaaTTGATTCTGCTTTCCCCGTAACATAGGCTTCTTCTTTTAAAAACATGTTTTATAATTCCATGACCCAAAAGGAAAAGACCCCT‑AAAACCCAAAAAAAAAAATCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg < 1:661957/141‑1 (MQ=255) ttGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGa > 2:713632/1‑150 (MQ=255) ttGTGCAAAAATTGGTTCTCCTTTCCCCGTGAAATGGGCGTCATCTGTACAAATCATGTCTTCTAATCCCATTGCCAAAGAGGCATAGGCCCCTAAAACCGAAATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg < 2:114593/149‑1 (MQ=255) ttGTGCAAAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATTTGTACTAATCATTTCTTATAATCCCATTCACCAATAGACATAGGCCCCTAAAACCGCAAAAAAAAACA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGa < 1:953523/150‑1 (MQ=255) tgtgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt > 2:133799/1‑151 (MQ=255) tgCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGt > 2:1109616/1‑151 (MQ=255) aaaatttGTATCTGTATTCCCCGTAACATAGGCGTCATTTGTACAAATAATGTTTTATAATTCCATTGACCAATAGGAAAAGGCACTTAAAAACGAAAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa < 1:77652/140‑1 (MQ=255) aaaatttGGATCTCCATTCCCCGTGACATAGGCTTCATTTGTATTAATCTTTTTTTCTAATCCAATTAACCAAAAGGCATGGCCCCCTAAAACCGAAAAAAAAAAAC‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttt < 1:906928/146‑1 (MQ=255) aaaaatgctaTCCGCATCCCACGGGACGTGGGCTTCATCTGTTCTAACCATTTTTTCTAATCCCTTTCCACAATAGAAAAAGCACCCTAAAACCGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttt < 1:758214/141‑1 (MQ=255) aGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATCCCATTGACAAATAGGCATAGGCACCTAAAAACGAAATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGt < 2:435303/148‑1 (MQ=255) aaaTTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt > 1:1008314/1‑151 (MQ=255) ttGGATCTGCTTCCCCCGTGACATAGGCGTCATCTGTACTAATCATTTTTTCTAATTCCATTGCCAAATAGGCATAGCCACCTAAAACCGAAAAAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAt < 2:4016/150‑1 (MQ=255) ggATCAGAATCCCCCGTACAAAAGGCGTCTTCTGTACAAATCATTTCTTATAATCCCATGGCCAAATAGAAAAAGCCACCAAAAAGCGAAAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa < 1:370457/147‑1 (MQ=255) aTCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGa > 1:115025/1‑150 (MQ=255) tcccgcGTGACTTGGGCTTCATCTTTACTAATCTTTTCTTCTAATTCTATACCCAAATGGAGATGGGCACTTGAAAACGCAAAAAAGAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa < 2:1160886/142‑1 (MQ=255) tcccccGTGACATAGGCGTCATCTGTACTAATAATTTCTTAAAATTCCATTGACCAATAGGCATAGCCACCTAAAACCGAAATAAAAAAAA‑TTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATc < 1:82657/148‑1 (MQ=255) tcccccGTGACATAGCCGTCATCTGTACAAAACATGTTTTCTAATCCGATTGACAAAGAGGCAGTGGCGCCAGAAAGCGCAATAAATAAAC‑CTCCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt < 2:404694/143‑1 (MQ=255) tcccccGAGAAATAGGCGCCATTTTTAATATTCATGTTTTTTAATTCAATTGACCAATAGTAAAAGCCACATAAAATCGAAATAAAAAAAA‑TTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt < 1:617456/144‑1 (MQ=255) ccccGTGACATAGGCGTCATCTTTACTAATCATTTCTTCTAATTCCATTGACCAATAGGCATAGGCCCCGAAAAACGAAATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt < 2:918814/149‑1 (MQ=255) ccaggggACATAGGCTCCATCTGCACAAATTATGTTTATTAATCCCATTGTCCAAGAGGAATAGGCACGTCAAACCGCAATAAATAAAC‑CTGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCt < 2:983085/143‑1 (MQ=255) gTGACAAAGGCGTCATCTGTACTAATCATGTCTTCTAATTCAATAGACAAAAAGGAATAGGCACCTAAAAACGAAATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTcc < 1:686059/150‑1 (MQ=255) aCATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt > 2:944349/1‑150 (MQ=255) agaGGCGTTATCTATTCAAATCATGTCTTCTAATCCCATGGACAAATGGACAGAGGCCCAAAAAACCGAAAAAAAAAAAC‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtat < 2:913900/148‑1 (MQ=255) aTAGGCTTCATTTTTACAAACCATTTCTTCAAATTCCATTGACCAATAGGCATAGGCCCCTGAAACCGAAAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtatt < 1:86735/151‑1 (MQ=255) ggCGTCTTCTGTACTAATAATTTCTTTAAATTCCATTGACCAATAGACAAAGGCACCTAAAAACGCAAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt < 1:233688/145‑1 (MQ=255) tattgtatACAAATAATGTCTTATATTACCATCAACAAAAAGAAAAAGGCACATAAAAACTAAATAAAAAAAA‑TTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtattat < 1:152914/139‑1 (MQ=255) tGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc > 1:1115849/1‑151 (MQ=255) tGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc > 1:681291/1‑151 (MQ=255) tGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtc > 1:749300/1‑151 (MQ=255) taccTAAACATGTCTTCTATTTCCTTTAACAAATAGGCATAAACACCGAAAATCGAAAAAAATAATA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAt < 1:596366/144‑1 (MQ=255) gTACTAATAATTTTTTCAAATTCCATTGACAAATAGGAATAGGCACCTAAAAACCAAAAAAAAAAAC‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCAttt < 2:558887/149‑1 (MQ=255) aCTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTtctc > 1:1121328/1‑150 (MQ=255) cATGTTTTCTAATCCCATTAACCAATAGGCATAGGCCCCTAAAAACAAAAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGa < 2:892192/149‑1 (MQ=255) cATGTCTACTAATCCCATTGACCAATAGGCATAGGCACCAGAAAACTAAATAAATAAAC‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGa < 1:1123966/149‑1 (MQ=255) tGTCTTATAATCCCATTCACCAATAGACAAAGGCACTTAAAACCGAAAAAAAAAAAA‑TTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATg < 1:172546/149‑1 (MQ=255) ttctAATTCCATGGCCAAATAGTAAAAGGCACCTAAAACCACAAAAAATAAAA‑CTTCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGa < 2:235007/146‑1 (MQ=255) tGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTAtgaattgaat > 1:168361/1‑150 (MQ=255) gACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATtgaatg > 2:231167/1‑150 (MQ=255) aggcataggcaCCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGc > 1:508062/1‑151 (MQ=255) ggcataggcaCCAAAAAACGAAATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGca < 1:946394/151‑1 (MQ=255) agccaCCTAAAACCGAAAAAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGca < 1:274400/143‑1 (MQ=255) caCCTAAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttct < 1:517139/151‑1 (MQ=255) cttaaaaaCGCAAAAAAAAAAA‑CAACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGcattca < 2:509526/141‑1 (MQ=255) ccTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttctt > 2:609116/1‑150 (MQ=255) aaaaCGCAATAAAAAAAC‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCAttcttc < 2:301332/147‑1 (MQ=255) acacAATAAAAAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTGATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTGATGAATTGAATGAAAAGAGCATTCATTCTTCATATCaa < 1:933594/148‑1 (MQ=255) cAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAac > 2:1154931/1‑150 (MQ=255) ataaataaAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac > 1:918497/1‑150 (MQ=255) ataaataaAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTACACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAacac > 1:790959/1‑150 (MQ=255) aaaaaaaa‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGt < 2:32402/151‑1 (MQ=255) aataaaA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTg > 2:947919/1‑151 (MQ=255) | TTATGATTAACGACAGTGACACTACCTGAGACTTGTGCAGAAATTGGATCTGCATTCCCCGTGACATAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAA‑CTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTATTATCTCTCATTTCTCATAGATGAAATTTATGAATTGAATGAAAAGAGCATTCATTCTTCATATCAAACACAGTG > NC_000913/2483128‑2483416 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |