Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,676,106 | 0 | C | A | 40.0% | 74.8 / 21.7 | 50 | L240F (TTG→TTT) | yphD | putative sugar ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base C (27/3); new base A (0/20); total (27/23) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.76e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAAC > NC_000913/2675959‑2676256 | cGTAATCCCCAGCACCCCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCcaaca < 1:678253/149‑1 (MQ=255) gTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACaaa > 2:745669/1‑150 (MQ=255) cACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCg > 1:455326/1‑151 (MQ=255) aCCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCgg > 2:799442/1‑151 (MQ=255) cAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑c < 2:687522/151‑1 (MQ=255) cAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACcgccgc > 2:296085/1‑151 (MQ=255) ccGAACAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCg > 1:446648/1‑151 (MQ=255) aaCAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGaa > 1:702167/1‑150 (MQ=255) aCAGGGAACCCGCGCCCGCCGA‑AAGGTGCCTTACCGCCGACCACGCCCGCGGCGATGACGTCAACCTCCAGCCCGTTTGCGGCCCCTGCGTTCCCAGACCCGAGGCGCGCGGCAAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGa < 2:622849/149‑1 (MQ=255) aCAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGaa > 1:98949/1‑149 (MQ=255) ggaacccgcccccgcggG‑AAAGTGCCGTCCCGCCGCCCACGACCGCGGCGACGCGGTCAACCCCCAGCCCGTTTGCGCCCCCTGCGTTACCAGACCGGAGGCGCGCCGCAAAAAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAgg < 2:446661/138‑1 (MQ=255) ggAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGt > 2:195104/1‑150 (MQ=255) ggacccggACCCCCGGAAAAGGGCCGAACCGCCGCCCACGCCCGCGGCGATGACGCAAAACCCAAGACGTTTGGCGCCACCTGTGTCCCCAGACCCGAGGCGCCCCCCAAAAAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTa < 2:716828/144‑1 (MQ=255) a‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTaa > 1:407628/1‑149 (MQ=255) ccGCGACCGCGGA‑AAGGTGCCTTCCCGCCGACCCCGCCCGCGGCGATGCCGTAAAACTCCAGCCCTTTTCCGCCCCCTGCGTCCCAAGAACCGGGGCGCCCCGCCAAAAAAA‑TCCCGGC‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGa < 2:1202183/151‑1 (MQ=255) cgcgACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTaa > 2:238810/1‑147 (MQ=255) gACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATa > 1:480187/1‑149 (MQ=255) aCCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATa > 1:912577/1‑148 (MQ=255) ccgccgA‑AAAGTGCCGTCCCGCCGACCCCGCCCGCGGCGATGACGTCAACCCCCAGACGTTTGGCGGCCCCTGCGTTACCAGACCCGAGGCGCGCCGCAAAAAAAA‑GGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATa < 2:187429/147‑1 (MQ=255) cgcggG‑AAAGTGCCGTCCCGCCGACCACGCCCGCGGCGATGACGTAAAACTCCAGACCGTTTGCGGCCCCTGCGTTACCAGAACCGAGGCGCGCCGCAAAAAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAAt < 2:692795/151‑1 (MQ=255) cgccgG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGAAAAGATAAGaa > 1:1134359/1‑150 (MQ=255) aaaGTGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGAAAACCCCCAGACCTTTGGCGGCACCTGCGTCCCAAAACCCGAGGCGCGCCGCAAAAAAAA‑GGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAc < 1:5996/151‑1 (MQ=255) aaaGGGCCGTCCCGCCGACCACGCCCGCGGCGATGACGCCAAACTCCAGACCGTTTGCGGCCCCTGCGTTACCAGACCCGAGGCGCGCCGCAAAAAAAA‑GGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATgcgc < 2:767267/149‑1 (MQ=255) aaGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCAcc > 1:557550/1‑151 (MQ=255) gTGCCGTCCCGCCGCCCACGCCCGCGGCGATGACGTAAAACCCCAGACCGTTTGCGGCCCCTGCGTTCCCAGACCCGAGGCGCGCCGCCAAAAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcg < 2:707998/151‑1 (MQ=255) ccGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCcgacg > 2:1126331/1‑151 (MQ=255) tACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAAc > 1:361166/1‑151 (MQ=255) cgccgcCCCCGACCGGGGCGATGACGTAAACCTCCAGCCCTTTTGCGCCCCCTGCGTTCCCAGACCGGGGGCGCGCCCCAAAAAAAA‑GGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGtt < 2:606424/151‑1 (MQ=255) ccgACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTg > 1:528073/1‑150 (MQ=255) ccACGCCCGGGGCGAGGACTTCAAACCCCAGACGTTTTGCGGCCCCTGCTTTCCAAGACCCGAGGCGCGCCCCAAAAAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGcc < 2:1064628/151‑1 (MQ=255) cACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCg > 1:263528/1‑151 (MQ=255) gcggcgATGCCGTCAACCCCCAGCCCGTTCGCGGCCCCTGCTTTCCCAGACCCGGGGCGCGCCGCAAAAAAAA‑TCCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACt < 2:860231/151‑1 (MQ=255) gcggcgATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAAc > 1:471894/1‑150 (MQ=255) gcgATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTgcg > 2:476522/1‑151 (MQ=255) cGTCAATCTCCACACGGTTAGCGGCCCCTGGGTTCCAAAACCCGCGGCGCGCCGCAAAAAAAA‑TCCCGCC‑CTCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGccgccg < 2:996857/150‑1 (MQ=255) cGTAAAACTCCAGCCGGTTTGCGCCCCCTGCGTTCCCAGACCCGGGGCGCGCCGCCAAAAAAA‑TGCGGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCgccgcc < 2:383431/149‑1 (MQ=255) tCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTAc > 2:493173/1‑151 (MQ=255) cAGACTTTTTGCGCCCCCTGCTTTCCAAGACCCGGGGCGCCCCCCCAAAAAAA‑TGCCGTT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTAcc < 1:578729/150‑1 (MQ=255) cAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCg > 1:1081564/1‑151 (MQ=255) cgttttGCGGCACGGGAGTTACCACAGCCGGGGCGGGCGGCAAACAAAA‑GCCCGGT‑AACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTAc < 2:554171/142‑1 (MQ=255) cGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaa > 1:1203997/1‑150 (MQ=255) ctcctgttttACCAGACCGGAGGCGCGCTCCCAAAAAAA‑GGCCGCT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaaaa < 2:698586/141‑1 (MQ=255) aCCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAAcc > 2:1108581/1‑151 (MQ=255) gCGTTCCCAAACCCGAGGCGCGCCCACAAAAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGa < 2:41849/149‑1 (MQ=255) ttACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGcc > 2:853422/1‑151 (MQ=255) aGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGa > 1:774858/1‑149 (MQ=255) aCCGGGGGGCCCCGCAAAAAAAAGTGCGGCT‑CCCCGCCGCTAATAACCCCGAAAGGTTAAAGATAAGAATCCGCGCCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAgg < 1:949637/150‑1 (MQ=255) ccGAGGCGCCCCCCAAAAAAAA‑AGCCGGT‑CCCCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTCCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAg < 2:111449/147‑1 (MQ=255) ggggCGCGCGCCAAAAAAAA‑GGCCGGC‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCCCCCGACGAACGTTGAGGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGc < 1:639121/145‑1 (MQ=255) cgcgcCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGc > 2:1167473/1‑151 (MQ=255) ccgcaAAAAAAA‑GCCCGTC‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGc < 2:544227/150‑1 (MQ=255) caccaaacaaAG‑GGCCGCG‑CACCGCCGCTAATAACCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTg < 1:514459/141‑1 (MQ=255) caaaaaAA‑AGCCCGTCCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtg < 1:641841/149‑1 (MQ=255) aacaaAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtgatg > 1:114398/1‑150 (MQ=255) caaAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAac > 2:522189/1‑151 (MQ=255) | CGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAA‑CCGCGACCGCCGG‑AAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAA‑TGCCGGT‑CACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAAC > NC_000913/2675959‑2676256 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |