Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	701,405	C→A	E64* (GAA→TAA)	nagC ←	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	701,405	0	C	A	100.0%	214.3 / NA	60	E64* (GAA→TAA)	nagC	N‑acetylglucosamine‑inducible nag divergent operon transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/25);  total (35/25)

CTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTT  >  NC_000913/701258‑701550                                                                                                                                                    |                                                                                                                                                  cttctGACAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTTGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGATTCGGTGACGATGGAGATAGCGCGGCGGCCTCCGGTGGAGGCCTGCTGATCAACTTAttt                                                                                                                                                >  1:225261/1‑151 (MQ=255)   tctGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAActtatt                                                                                                                                                 <  1:1107569/148‑1 (MQ=255)      gCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGAt                                                                                                                                             <  2:1029695/149‑1 (MQ=255)      gCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATc                                                                                                                                            <  1:906302/150‑1 (MQ=255)            aCTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCcgc                                                                                                                                     >  2:753054/1‑151 (MQ=255)             cTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCcgcg                                                                                                                                    >  2:257337/1‑151 (MQ=255)              tttGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCGCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCcgcg                                                                                                                                    >  1:1165949/1‑150 (MQ=255)               ttGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCcgcg                                                                                                                                    <  2:480265/149‑1 (MQ=255)               ttGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGt                                                                                                                                   <  2:422897/150‑1 (MQ=255)                 gctgctGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTc                                                                                                                                 <  2:560663/150‑1 (MQ=255)                 gctgctGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCg                                                                                                                                <  2:831613/151‑1 (MQ=255)                    gctgAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATa                                                                                                                             <  1:99651/151‑1 (MQ=255)                       gagaTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAg                                                                                                                           >  1:911340/1‑150 (MQ=255)                         gaTCAATCAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATCGCGTGGAAATTGCGGGTTTCTGTGACGATGGAGAGAGCGGGGGGGCTGCCGGTCGAGGCCTTCTGATCAACTTATTTGATCAGTGCGGGTTCGATAAg                                                                                                                           <  1:109253/148‑1 (MQ=255)                          aTCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTg                                                                                                                        >  2:928507/1‑150 (MQ=255)                               aCAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGt                                                                                                                  >  2:874589/1‑151 (MQ=255)                                  gagTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAAt                                                                                                               >  2:983716/1‑151 (MQ=255)                                      gATGGCGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGGAATTTTg                                                                                                           >  1:1204498/1‑151 (MQ=255)                                       aTGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTgg                                                                                                          >  2:1104798/1‑151 (MQ=255)                                           tggCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTAc                                                                                                      >  2:53230/1‑151 (MQ=255)                                              cGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCt                                                                                                   >  1:367949/1‑151 (MQ=255)                                               gTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTg                                                                                                  <  1:928678/151‑1 (MQ=255)                                                tCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTgg                                                                                                 >  1:1126779/1‑151 (MQ=255)                                                    gacgacCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCggg                                                                                             >  2:1178605/1‑151 (MQ=255)                                                     acgacCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCgggg                                                                                            <  1:323289/151‑1 (MQ=255)                                                      cgacCAAGCCGTACGCCGATTGCGTGGAAAGTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCggggg                                                                                           <  2:692365/151‑1 (MQ=255)                                                       gacCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGc                                                                                          >  2:239847/1‑151 (MQ=255)                                                       gacCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGc                                                                                          >  2:1154203/1‑151 (MQ=255)                                                       gacCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGc                                                                                          >  2:1059944/1‑151 (MQ=255)                                                           aaGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGTCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGc                                                                                      >  2:863437/1‑151 (MQ=255)                                                           aaGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGc                                                                                      >  1:35098/1‑151 (MQ=255)                                                                  aCGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCttt                                                                               <  2:580552/151‑1 (MQ=255)                                                                  aCGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCttt                                                                               <  2:535895/151‑1 (MQ=255)                                                                  aCGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCtt                                                                                <  2:375971/150‑1 (MQ=255)                                                                   cGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTg                                                                              <  1:53229/151‑1 (MQ=255)                                                                           gCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCaa                                                                      <  2:421273/151‑1 (MQ=255)                                                                           gCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCa                                                                       <  1:756185/150‑1 (MQ=255)                                                                            cGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAACCTGGCTTTGCTCGGCAAt                                                                     >  1:343644/1‑151 (MQ=255)                                                                                gAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTg                                                                  >  1:227384/1‑150 (MQ=255)                                                                                  aaTTGCGGGTTTCGGTGACGATGGAGATAGCGCCGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAAt                                                               >  1:485667/1‑151 (MQ=255)                                                                                        gggTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCcgcg                                                          >  1:1157832/1‑150 (MQ=255)                                                                                              cGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTg                                                    <  1:792641/150‑1 (MQ=255)                                                                                                     gATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTAc                                            >  1:196978/1‑151 (MQ=255)                                                                                                      aTGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACt                                           >  2:710999/1‑151 (MQ=255)                                                                                                      aTGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACt                                           <  1:22967/151‑1 (MQ=255)                                                                                                             tAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATATGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAAt                                    >  1:984579/1‑151 (MQ=255)                                                                                                                      ggcCCCCGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGAt                            >  2:1071414/1‑150 (MQ=255)                                                                                                                         ccccGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAAc                        >  2:120746/1‑151 (MQ=255)                                                                                                                           ccGGTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAAccg                      <  1:257330/151‑1 (MQ=255)                                                                                                                             ggTGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAAccgcc                    >  2:707925/1‑151 (MQ=255)                                                                                                                               tGGAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACcgccgc                  >  2:360422/1‑151 (MQ=255)                                                                                                                                ggAGCCCTGCTGATCACCTTATTTGACCAGCCCGCGTTCGATAAGTGGACCGGTAATTTTGATTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTAAATCCGCGATATTGGCCCGTACTGGTAAATCGGGCGATAAACcgccgc                  <  1:790297/150‑1 (MQ=255)                                                                                                                                 gAGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCcgcg                 >  1:90620/1‑150 (MQ=255)                                                                                                                                  aGGCCTGCTGATCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCt               >  2:651104/1‑151 (MQ=255)                                                                                                                                      ctgctgATCAACTTATTTGAACAGCCCGCGCTCGATAACCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCTACATTGGCCCGAACTGGTCAATCAGGCGATAAACCACCGCGATg              >  1:517484/1‑148 (MQ=255)                                                                                                                                            ataaaCTTATTTGATCATCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACCGGTCAATCAGGCGATAAACCGCTGCGCTGTTAAGCTGt     >  1:242847/4‑151 (MQ=255)                                                                                                                                            aTCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGCCGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCt       <  1:653472/149‑1 (MQ=255)                                                                                                                                            aTCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTg      <  1:232179/150‑1 (MQ=255)                                                                                                                                            aTCAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGt     <  2:780461/151‑1 (MQ=255)                                                                                                                                              cAACTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGGCAATCAGGCGATAAACCGCCGCGCTGTTACGCTGttt   >  2:804519/1‑151 (MQ=255)                                                                                                                                               aaCTTATTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGtttt  <  1:246745/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CTTCTGCCAGCACTTTGCTGCTGAGATCAAACAGAGTGATGGTGGCGTCATGACGACCAAGCCGTACGCCGATTGCGTGGAAATTGCGGGTTTCGGTGACGATGGAGATAGCGCGGCGGCCCCCGGTGGAGGCCTGCTGATCAACTTCTTTGATCAGCCCGCGTTCGATAAGCTGACGCGTAATTTTGGTTACGCTGGCGGGGGCAAGCTGGCTTTGCTCGGCAATCTGAATCCGCGAGATTGGCCCGTACTGGTCAATCAGGCGATAAACCGCCGCGCTGTTAAGCTGTTTT  >  NC_000913/701258‑701550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑