Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,460,8710TG41.2% 91.2 / 11.7 68Y597S (TAC→TCC) nrdDanaerobic ribonucleoside‑triphosphate reductase
Reads supporting (aligned to +/- strand):  ref base T (14/26);  new base G (28/0);  total (42/26)
Fisher's exact test for biased strand distribution p-value = 5.53e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTT  >  NC_000913/4460725‑4461021
                                                                                                                                                  |                                                                                                                                                         
cGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAgg                                                                                                                                                      >  1:509521/1‑151 (MQ=255)
 gTAGCTCTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGgc                                                                                                                                                     <  1:8979/151‑1 (MQ=255)
  tAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTa                                                                                                                                                          >  2:624088/1‑146 (MQ=255)
       cTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGgcgc                                                                                                                                                   >  2:154240/1‑147 (MQ=255)
         catcaATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCa                                                                                                                                               <  1:861012/149‑1 (MQ=255)
          atcaATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAg                                                                                                                                            <  1:912449/151‑1 (MQ=255)
          atcaATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGGAGGCGCGTCaaa                                                                                                                                             >  2:447029/1‑150 (MQ=255)
            caATCGGTGTATTGGTGCCGTAGTACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTc                                                                                                                                          <  2:1114047/151‑1 (MQ=255)
            caATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGGTTTCAAGTc                                                                                                                                          >  1:1004822/1‑151 (MQ=255)
             aaTCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCg                                                                                                                                         <  2:1247278/151‑1 (MQ=255)
                cGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTaaaggggct                                                                                                                                                  >  2:133922/1‑133 (MQ=255)
                cGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGAt                                                                                                                                       <  1:390459/150‑1 (MQ=255)
                  gtgtATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCtt                                                                                                                                    <  1:1040839/151‑1 (MQ=255)
                  gtgtATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCtt                                                                                                                                    <  2:1218590/151‑1 (MQ=255)
                  gtgtATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGGAGGCGGCTCAAAgctacaatt                                                                                                                                    >  2:337127/1‑143 (MQ=255)
                   tgtATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCtt                                                                                                                                    <  2:187927/150‑1 (MQ=255)
                   tgtATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGCGCTCCaaatccacttt                                                                                                                                     >  2:702439/1‑142 (MQ=255)
                    gtATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTg                                                                                                                                   <  1:855904/150‑1 (MQ=255)
                     tATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGCGCCTCCAAACAGATCtttt                                                                                                                                  >  2:870254/1‑148 (MQ=255)
                      aTTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGGGCTTCCAAAACGATc                                                                                                                                      >  1:808568/1‑145 (MQ=255)
                      aTTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCTTCAAAATCGATCtttt                                                                                                                                  >  1:977645/1‑147 (MQ=255)
                        tGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGGGCGTCAAAATCgccctttttggc                                                                                                                              >  2:237367/1‑140 (MQ=255)
                        tGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCGTCaaaaatgaactt                                                                                                                                    >  2:1224682/1‑136 (MQ=255)
                         ggTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTa                                                                                                                              <  2:360237/150‑1 (MQ=255)
                            gCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACggg                                                                                                                          <  2:778998/151‑1 (MQ=255)
                             ccGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGt                                                                                                                        <  2:307066/151‑1 (MQ=255)
                                 aaTACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCa                                                                                                                     <  1:491773/150‑1 (MQ=255)
                                      ggTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCTTCACAGTCGATCTTTTCGTACGGGGTTACctt                                                                                                                 >  2:107751/1‑149 (MQ=255)
                                       gTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACcttctt                                                                                                              <  1:1075935/151‑1 (MQ=255)
                                                  tAGCTGTAATCCCAGACATCTGCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCcaca                                                                                                      <  2:439125/147‑1 (MQ=255)
                                                    gCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGCAGAGGCATCAAAATCGATTGTTTCTTAAGGGTTTCATCTCCTCTCCACAtaca                                                                                                  >  1:232221/1‑146 (MQ=255)
                                                       gTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCTTCAAAATCGATATTTTCGTACGGGGTTCCCTTTTTTTCCCCCTCGCGGTgg                                                                                             >  1:461712/1‑151 (MQ=255)
                                                          aTCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGGAGGGGCTTCAAAAGCGGTCTTTTTGTTCGGGTTCCCCCTCTTCTCCCCCTCCAgggg                                                                                              >  2:813351/1‑145 (MQ=255)
                                                          aTCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCCTCAAAAACGATCTTTTCGTACGGGGTCACCTTCTTCTccccctccggggggga                                                                                            >  1:856107/1‑136 (MQ=255)
                                                              cAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGt                                                                                      <  2:1194574/151‑1 (MQ=255)
                                                               aGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGCGGTTCAAAAACGATCTTTTTGTACGGGGTCACCCTCTTTTCCCCCTCGCGGGGGgaaaaggt                                                                                     >  2:402630/1‑146 (MQ=255)
                                                                  cATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGGTTCCAAAACGATCTTTTTGGACGGGGTGACCCTCTTCTCCCCCTCGAGGGGGGAAATGt                                                                                      >  2:1262304/1‑147 (MQ=255)
                                                                   aTCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGGAAGCGGGTCAAAGAAGCTCTTGTCGTACGGGTTCCCCTTCTTTTTCCCATCGAGGTGGaagacgtt                                                                                     >  2:368071/1‑141 (MQ=255)
                                                                    tCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGGGCCTCCAAATCGATCTTTTCGTACGGGGTCTCCCTCTTCTCCCCATCCAGGGGGGAAATGtt                                                                                     >  1:983171/1‑146 (MQ=255)
                                                                    tCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAAGCGCTTTCCAGTAGATCTTTTCGTACCGGTTCTCCTTCCTCTCCCCAACCCGGGGGGAACTGTTGgt                                                                                  >  1:1002464/1‑149 (MQ=255)
                                                                       tCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAATCGATCTTTTCGTACGGGGTCACCTTCTTCTCCACATCGAGGGGGGAAATGTTGGTgtagt                                                                             >  1:1260838/1‑151 (MQ=255)
                                                                              ccTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGCTTCAAAAACGATCTTTTCGTAAGGGGTCACCTTCTTTTCCCCCTCGGGGGGGGAAATGTTTGGGTTGTaaacctt                                                                      >  1:652326/1‑146 (MQ=255)
                                                                                 tCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTAt                                                                    <  2:1248596/150‑1 (MQ=255)
                                                                                 tCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGGCTCAAAAACCAACTTTTTGGACGGGGTTAACCTCTTTTCCCCCTCGGGGGGGGAAATGTTTGTGTAGGaaacctt                                                                      >  1:163599/1‑143 (MQ=255)
                                                                                   aGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCTTCTAAAACGAACTTTTCGTAAGGGGTGCCCTTCTTTTTCCCATCCCGGGGGGAAAAGTTGGTGGTGTAACCTTTTTCgg                                                                 >  1:918176/1‑151 (MQ=255)
                                                                                    ggTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGt                                                                <  2:762188/151‑1 (MQ=255)
                                                                                     gTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGTGGTTCAAAAAAGAACTTGTCGGTCGGGGTGACCCTCTTTTCCCCACCGCGGGGGGAACTGTTTGTGTTGTTACCTTTTTAGGtt                                                               >  2:1128813/1‑150 (MQ=255)
                                                                                      ttGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGGGCTTCAAACTCGATCTTTTCGTACGGGGTTACCCTTTTCTCCCCAACCAGGGGGGAAAAGTTGGTGTTGTAAACTTTTTCGGTcc                                                              >  2:607533/1‑150 (MQ=255)
                                                                                        gtgCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAAACGATCTTTTCGTACGGGGTCACCTTCTTCTCCCCATCCAGGGGGGAACTGTTGGTGTAGTAAACTTTATCGGTCAc                                                             >  1:1084197/1‑150 (MQ=255)
                                                                                            tGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGGGGTTTAAAATAAAACTTTGTGTTCGGGGTTCCCCTCTTCTCCCCATCAAGGGGGGAAAAGATGGTGTGGTAACCcttttttgtccagccc                                                         >  2:751619/1‑137 (MQ=255)
                                                                                              aaTGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGc                                                      <  2:183903/151‑1 (MQ=255)
                                                                                              aaTGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCCTCCAAATCGATCTTTTCGGACGGGGTCACCTTCTTTTCCCCAACCAGGGGGAAACTTTTTGTGTAGGAAACCTTATCTGGCACGccc                                                         >  1:113261/1‑148 (MQ=255)
                                                                                                       aTACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAATGCGGTTCAAAGTTCATTTTGTCGGACGGGTTCTCCCTCTTTTCCCCAACCGGGGGGGGAACGTTTGGTTTGGAACCTTTTTTGGTCACACCCCGGACCCCGc                                               >  1:504745/1‑149 (MQ=255)
                                                                                                        tACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAAGCGCTTCAAAATCGCTCTTGTTGGACGGGGTCACCTTCTTTTCCCCATCGAGGGGGGAACTGTTTGTGGAGTAAACTTTTTTGGTCACGCCCGGgcccaccc                                               >  2:1240820/1‑146 (MQ=255)
                                                                                                            cGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAAc                                          <  1:2509/149‑1 (MQ=255)
                                                                                                            cGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACt                                         <  2:1254375/150‑1 (MQ=255)
                                                                                                                  aGCAAATGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCTTCCAAAGCGAGCTTTTCGTACGGGGTTACCTTCTTTTCCACAACGAGGTGGGAAACGTTGGTGTAGTAAACTTTTTCCGTTACGCCCCGCACCCCGCCCAAATcagc                                     >  1:112961/1‑148 (MQ=255)
                                                                                                                    cAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCTAAAATGATCTTTTCCTACAGGGTCTCCTTTTTTTCCACCTCCAGGAGGAAAATTTTGGTGTTATAAACTTTTTCGGGTACGCCCGGgacccacccccacaccc                                      >  1:1046099/1‑132 (MQ=255)
                                                                                                                     aaaTGAAACCACCGTTCGCCAGCGGCGGGGAAGGCGCTTTAAAATCGATCTTGTCGTACGGGGTCACCTTCTTCTCCACATCGAGGGGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCCGCACCACGCCCAACTCAGCAGTa                                 >  1:775237/1‑149 (MQ=255)
                                                                                                                        tGAAACCACCGTTCGCCAGCGGCGGGTAAAGCGCGTCAAAATCGATCTTGTCGGACCGGGTCACCTTCTTCTCCCCATCGAGGGGGAAACAGTTGGTGGAGGAAACTTTTTCGGTCAAGCCCCGCACCACGCCCAACCCAGCAGGATCg                              >  1:184319/1‑149 (MQ=255)
                                                                                                                              caccGTTCGCCAGCGGCGGGGAAGGCGCCTCAAAAGCGATCACGTCGGATACGGTTACCTTCTTATCCACCCCCAGGGGGGGAAAGTTTGTGTGGTAAACCTTTTCGGTCACGCCCCCCACCACACCAAAAACAGCAGTATCGCGACgg                        >  2:965871/1‑149 (MQ=255)
                                                                                                                                        cAGCGGCGGGTAAAGGGCTTTCAAGTCGATCTTTTCGTACGGGGTTCCCTTCTTCTCCACATCGAGGGGGAAACTGTTGGGGTTGTAACCTTTTTTGGTCACGCCCCGCCCCACCCCAAACTCAGCAGTATCGAGACGGCAGAAGCGAt              >  2:236747/1‑149 (MQ=255)
                                                                                                                                          gcggcgGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGc           <  2:468357/150‑1 (MQ=255)
                                                                                                                                            ggcggGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGcac         <  2:376896/150‑1 (MQ=255)
                                                                                                                                             gcggGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGcac         >  1:798577/1‑149 (MQ=255)
                                                                                                                                                ggTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGtt    <  2:120778/151‑1 (MQ=255)
                                                                                                                                                ggTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGtt    <  2:977606/151‑1 (MQ=255)
                                                                                                                                                 gTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGttt   >  1:653644/1‑151 (MQ=255)
                                                                                                                                                  tAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGtttt  >  1:971318/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                         
CGTAGCACTCATCAATCGGTGTATTGGTGCCGTAATACGGTACATGCTGATAGCTGTAATCCCAGACATCTTCCAGCGCCTTCAGGTTGTGCTGAATGTTTGGATACTCGCCGTAGCAAATGAAACCACCGTTCGCCAGCGGCGGGTAAGGCGCTTCAAAGTCGATCTTGTCGTACGGGTTCACCTTCTTCTCCACATCGAGGTGGAAACTGTTGGTGTAGTAACCTTTATCGGTCACGCCCGGCACCACGCCAAACTCAGCAGTATCGAGACGGCAGAAGCGATCGCACAGGTTTT  >  NC_000913/4460725‑4461021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: