| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 380,022 | (G)10→11 | intergenic (‑141/+47) | frmR ← / ← yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 380,012 | 1 | . | G | 88.1% | 136.6 / 11.4 | 43 | intergenic (‑131/+57) | frmR/yaiO | regulator protein that represses frmRAB operon/outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base . (4/1); new base G (21/16); total (25/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA > NC_000913/379865‑380154 | tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaa‑‑a < 2:1198640/148‑1 (MQ=255)tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑ggg > 2:221028/1‑151 (MQ=255)tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑gg < 1:1059876/150‑1 (MQ=255) ccGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑ggg < 2:913777/150‑1 (MQ=255) ccGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑ggg < 2:374757/150‑1 (MQ=255) cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑g < 2:347828/147‑1 (MQ=255) gggCGTTTCGCACCCCTTCACGTGGATGTAAGATTCTAGAGGGGGGTATTCTATAT‑GTCAATGCATCCCCCCCTATAGTACAATACACGGGAGAATAAAATTTATCCGGTGAATGCGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑gggggg‑‑ggggg < 1:1050290/148‑2 (MQ=255) ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑gggggg‑‑ggggggct > 2:723633/1‑146 (MQ=255) tttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AG‑‑GGGGGG‑‑GGGG‑‑CTAATCgg > 2:469283/1‑151 (MQ=255) tttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑gggggg‑‑ggggggctaatcg > 2:972538/1‑142 (MQ=255) tttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑gggggg‑‑ggggggctaatcg > 1:577816/1‑142 (MQ=255) cctcTTCATTCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGGGGGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGgcc > 2:877786/3‑151 (MQ=255) atcTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGgccgc > 1:856937/1‑151 (MQ=255) atcTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAAAGTGGTCGGAAAACAAAGAGGAA‑‑AG‑‑GGGGGG‑‑GGGG‑‑CTAAAGGGAGGGGAAGgccccc > 2:821124/1‑151 (MQ=255) atgaattaacctaaagggggTATTCT‑TATAGTTAAATCCTACCCCCCCTAAGTAAAAAACCCGGGGAAAAAAAATTTTTCCGGGGGAAGGGGGCGGAAAAAAAAAGGGAA‑‑AAG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGAt < 1:336024/136‑1 (MQ=255) aaaatccTAAAGGGGGGTTTTTTAATT‑TTAAAACCATCCCCCCCTATAGTTAAAAACCGTGGAAAAAAAAATTTATCCGGGGAATGTGGTCGGAAAAAAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGAt < 2:20392/142‑1 (MQ=255) atatACTATAGGGGGGTATTATAAAT‑GTCAATGCATCCCCCCCTATAGTATAAAACACAAGAGAATAAAATTTATCCGGGGAATGTGGTCGGAAAACAAAGAGAAA‑‑AG‑‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg < 2:321147/151‑1 (MQ=255) ataCTATAGGGGGGTATTCTATAT‑GTCAAGGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AG‑‑GGGGGG‑‑GGGG‑‑CTAATGGGCAGGGAAGGACGCCCCGGATAgcgg > 1:823374/1‑149 (MQ=255) ataGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc > 2:870411/1‑150 (MQ=255) ataGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGAAGGGAAGGCCGCCCCGGATAGCGggcggc > 2:688760/1‑150 (MQ=255) ggggtgTAGTCTTTAT‑TTAAAGGCTTCCCCCCTTTTAATACAAAAACCAGGAGAAAAAAATTTATCCGGCAAAGGGGCTCGGAAAACAAAGGGAAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc < 1:1033070/144‑1 (MQ=255) ggggggTTTTCTATAT‑TTCAATGCATACCCCCCTATAGTATAAAACACTGGAAAAAAAAATTTATCCGGTGAATGTGGGCGGAAAAAAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAg < 2:50050/149‑1 (MQ=255) ggggggTTTTCAAATT‑GTCAATGAATACCCCCCTATTATATAAAAACGTGGAAAAAAAAATTTTTCCGGGGAATGGGGGCGGAAAAAAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa < 2:646925/150‑1 (MQ=255) gggggTATTCTATAG‑GGCAATACATCCCCCCCTATAATAAATAACACTGGAGAAAAAAATTTATCCGGGGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggc < 1:233016/143‑1 (MQ=255) aTTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑GGGGGG‑‑GGGGGCCTATTCGCGAGGGAAGGCCGCCCCGGATGGCGGGCGGCAGAGGGAAt > 2:1104775/1‑149 (MQ=255) tCTATAT‑GTCAATGCAGACCCCCCTATAGTAAATAAAACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAAGGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg < 1:326440/150‑1 (MQ=255) tCTATAT‑GACAATGCAACCCCCCCTAAATTATAAAACAGGGAAAAAAAAAATTTAGCGGGGGAATGTGGGGGGAAAAAAAAGGGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc < 2:725654/148‑1 (MQ=255) tat‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt > 2:536718/1‑150 (MQ=255) at‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑GGGGGG‑‑GGGGGCCTAACCGGCGGGGAAGGCCGCCCCGGGTACGGGGCCGAAGAAGGAGTCAGAttt > 1:1084852/1‑149 (MQ=255) gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc > 2:1158366/1‑150 (MQ=255) cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AG‑‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc > 2:900501/1‑151 (MQ=255) cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGT‑GTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg > 2:359745/1‑149 (MQ=255) aaaCCCCCTTATATTATTAAACACTGGAGAAAAAAATTTATCCGGGGAATGGGGTGGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa < 1:869114/146‑1 (MQ=255) gTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt > 2:365614/1‑150 (MQ=255) tataACACGGGAGAATAAAATTTATCCGGTTAATGTGGTCGGAAAACAAGGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt < 1:972000/147‑1 (MQ=255) tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGGGGGCGGCAGAAGGAATCAGAATTTCCAGGACAGACGGGCTGCAAGTTGCAGACGGTTAAAatcat > 2:1087632/1‑150 (MQ=255) aaaaTTTACCCGGTAAATGGGGTCGAAAAACAAAGAAAAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatcatc < 2:1163006/150‑1 (MQ=255) aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCAGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCgg > 1:968885/1‑151 (MQ=255) aaTTTATCCGGTGAATGTGGTCGGAAAACAACGTGGAA‑‑A‑‑‑GGGGGG‑‑GGAC‑‑ATAATCGGCCGGGAAGGCGTGCATGGATAGCGGCTGGCCGAGGGGCAGAAAATTTCGAGTTCTATCGGGGTGAAAGTTGCGGCGCGTTAAAATCATCGGTTg > 1:654595/1‑151 (MQ=255) tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTg > 2:780337/1‑149 (MQ=255) tCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATAGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTgggg > 1:889049/1‑150 (MQ=255) ccGGTGAATGGGGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgt < 1:59199/150‑1 (MQ=255) gTGAGTGTGGTGGAAAAAAAAAGAGGAAGGG‑‑‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtgt < 2:141403/149‑1 (MQ=255) tGAATGGGGTCGAAAAAAAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg < 2:751247/150‑1 (MQ=255) gtgGTCGGAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAcc > 2:443560/1‑149 (MQ=255) ggAAAACAAAGAGGCA‑‑AGG‑GGGGGG‑‑GGGG‑‑CAAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGCATTTCCAGGTAAGACGGGCTGCAAGTGTCAGACCGTTAAAATCTTCGGTTGGCGTGTCGTACCACACttt > 1:650826/1‑150 (MQ=255) gAAAACAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc > 1:97617/1‑151 (MQ=255) aaaaCAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTa < 1:916267/149‑1 (MQ=255) aaCAAAGAGGAA‑‑AGG‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGGGGGCGGCAGAAGGAATAAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCt > 2:796670/1‑150 (MQ=255) aaaGAGGAA‑‑AG‑‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCg > 1:276459/1‑150 (MQ=255) aaGAGGAA‑‑AG‑‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTc < 2:763111/151‑1 (MQ=255) aaGAGGAA‑‑A‑‑‑GGGGGGGCGGGG‑‑CTAAACGGCAGGGAAGGCCCCCCCGGATAGCGGGCGGGAGAAGGAACCAGAATTTCCAGGTCAGCCGGGCCGCAAGTTGCAGACCGTTAAAATCATCGGGTGGGGTGCCGCACCCCCCTTTACCTGcc > 1:32678/1‑149 (MQ=255) gagGAA‑‑AG‑‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCa < 1:469269/150‑1 (MQ=255) | TCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATAT‑GTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAA‑‑A‑‑‑GGGGGG‑‑GGGG‑‑CTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTCA > NC_000913/379865‑380154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |