Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,245,2340GT41.1% 116.2 / 37.9 73L25F (TTG→TTT) ycgYuncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (7/36);  new base T (30/0);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 4.39e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245091‑1245384
                                                                                                                                                |                                                                                                                                                           
atCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTggg                                                                                                                                                       >  2:78922/1‑148 (MQ=255)
  ccGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGGCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCtt                                                                                                                                                   <  1:93640/151‑1 (MQ=255)
  ccGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCtt                                                                                                                                                   <  1:682103/151‑1 (MQ=255)
   cGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTg‑cg                                                                                                                                                      >  2:792192/1‑147 (MQ=255)
      tAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCCTA‑tt                                                                                                                                               >  2:903305/1‑150 (MQ=255)
         aGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCCG‑CGCTTA‑TTGAAc                                                                                                                                           <  1:1081808/151‑1 (MQ=255)
          gTCGGTTCCCGGTAACAACAACAGAATCCTATTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACa                                                                                                                                          <  2:662677/151‑1 (MQ=255)
              gTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAAGAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTa                                                                                                                                      <  1:307348/151‑1 (MQ=255)
                 cccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGa                                                                                                                                    <  1:215838/150‑1 (MQ=255)
                  ccGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAAt                                                                                                                                  <  1:411918/151‑1 (MQ=255)
                    ggTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTg                                                                                                                                <  2:68434/151‑1 (MQ=255)
                        acaacaacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCa                                                                                                                            <  1:185454/151‑1 (MQ=255)
                            caacaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑Tcc                                                                                                                       <  2:816768/151‑1 (MQ=255)
                              acaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCtt                                                                                                                     <  2:1209621/151‑1 (MQ=255)
                              acaGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑CTTTGGATG‑CGGTTG‑TTGTAAAATTATAAATGATGAA‑TCCtt                                                                                                                     >  2:863944/1‑151 (MQ=255)
                               caGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑CGCTTA‑TTGAACATTTAGAAATTATCAA‑TCCtt                                                                                                                     >  2:303049/1‑150 (MQ=255)
                               caGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCCTT‑TTTTAAATTTAAAATTTATCAA‑TCCtt                                                                                                                     >  1:1084481/1‑150 (MQ=255)
                                     ccTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCTTT‑TTTAACATTTAGAATTGATTAA‑TCCTTCt                                                                                                                   >  1:185676/1‑146 (MQ=255)
                                        tttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTT‑AAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGAt                                                                                                            <  1:507258/149‑1 (MQ=255)
                                         ttttAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑CGCTTT‑TTTAAAATTTAGAATTTATCAA‑TCCTTTTGGCAGAtt                                                                                                           >  1:853336/1‑150 (MQ=255)
                                              attattGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑CGCTTA‑TTTAAAATTTTGGATTTATCAA‑TACTTCTGGCAGATTaaagc                                                                                                      >  2:666957/1‑147 (MQ=255)
                                                  ttGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑CTTTTGTTG‑CGCTTA‑TTTAACATTTTGAATTTTTCAA‑TCCTTTTTGCGGAATAAAGCaaaa                                                                                                  >  2:741946/1‑150 (MQ=255)
                                                   tGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGGTTTTTGGCGG‑GGTTTT‑TTTAA‑AATTATAATTTAGTAA‑ACCTTCTGGGAGAATAAGACcaaa                                                                                                  >  2:812311/1‑149 (MQ=255)
                                                        cGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑GGCTTT‑TTTAACATTTTGAATTTATTAATTCCTTTTGG‑AGATTAAAGCAAAATAccc                                                                                             >  1:528922/1‑149 (MQ=255)
                                                        cGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCTTA‑TTTAAAAATTAGAAATTATCAA‑TCCTTCTTGCCGATTAAGCCcaaaaaacc                                                                                             >  1:770584/1‑144 (MQ=255)
                                                           tgttTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAACTGGGGCGGATAGTGAGGCGG‑GTTTTGCTG‑CGCTTC‑TTGTACATTTTGAATTTCTTAA‑TCCTTCTGGCCGATTATGGCCAAATAaccccttg                                                                                        >  1:763871/1‑146 (MQ=255)
                                                             tttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTTCTGCCCCTTT‑TTTAAC‑TTTATAATTGGTTAA‑ACCTTATTGCCCATTAAATCAAAATAACCTTTTggg                                                                                      >  2:677960/1‑150 (MQ=255)
                                                             tttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTTAAAATTTAGAATTGATCAA‑ACCTTCTTGCAGATTAAGCCAAAAAAACCGTTCgg                                                                                       >  2:293628/1‑150 (MQ=255)
                                                              ttttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGc                                                                                      <  1:211784/150‑1 (MQ=255)
                                                               tttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑CGCTTA‑TTGTAAATTTAGAATTGATTAA‑ACCCTCTGGGAGATTAAGGCaaaaaaaccctttgg                                                                                       >  1:229519/1‑137 (MQ=255)
                                                               tttGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCTTA‑TTTAACAATTAGAATTTATCAA‑TTCTTCTGGCAGATTTAGTCAAAAAAACCGTTCg                                                                                        >  1:350560/1‑147 (MQ=255)
                                                                  ggTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCCTA‑TTTAACATTTTGAAATGATCAA‑TCCTTCTTGCAGATTTAGCCAAAAAAACCGTTCGGCaa                                                                                    >  2:477039/1‑148 (MQ=255)
                                                                   gtgtGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑GGCTTA‑TTTAAAAATTTGAAATTATTAA‑ACCTTCTTGCAGATTTAGCCAAAAAACCCGTTCGGCaa                                                                                    >  1:1198926/1‑147 (MQ=255)
                                                                    tgtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑CGCTTT‑TTTAACATTTTGAAATGATTAA‑ACCTTCTGGCAGATTAAACCAAAAAAACCCTTCGGCAATG‑Ctt                                                                              >  1:669308/1‑151 (MQ=255)
                                                                    tgtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGGTG‑CGCTTT‑TTTAACATTTAGAATTGATCAA‑ACCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑Ctt                                                                              >  2:210139/1‑151 (MQ=255)
                                                                     gtgATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑Ctt                                                                              <  1:1011182/150‑1 (MQ=255)
                                                                          tCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑CGCTTT‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAACCAAAATAACCGTTCGGCGATG‑Ctttcgtt                                                                         >  1:859126/1‑145 (MQ=255)
                                                                          tCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCTTT‑TTTAACATTTATAATTGATTAA‑TCCTTCTGGCAGATTAAGCCAAAAAAACCGTTTCGCAATG‑GTTCAGt‑t                                                                        >  1:708881/1‑150 (MQ=255)
                                                                           cAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑Ta                                                                       <  1:587246/150‑1 (MQ=255)
                                                                            aGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTTAAAACTTAAAAATTTTTAA‑CCACTCTGTCGGGTTTAGCCAAAATAaccagtttgtcaagctt                                                                               >  1:103709/1‑129 (MQ=255)
                                                                              aaaaTAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCTTA‑TTTAACATTTAGAAATTATCAA‑TCCTTCTGGCAGAATAAACCAAAAAAACCGGTCGGCAAAG‑CTTCAGT‑TAcgcg                                                                   >  2:828457/1‑151 (MQ=255)
                                                                               aaataaatATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGa                                                                  <  1:909581/151‑1 (MQ=255)
                                                                                aataaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCGTCTGGCAGATTAAGCCAAAATGCCCGTTCGGCAATG‑CTTCAGT‑TAcgcgga                                                                 <  1:578000/151‑3 (MQ=255)
                                                                                  taaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAag                                                                <  1:343304/150‑1 (MQ=255)
                                                                                   aaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAagag                                                              <  2:188676/151‑1 (MQ=255)
                                                                                   aaataTTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAaga                                                               <  1:1121839/150‑1 (MQ=255)
                                                                                         tttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCTTA‑TTGAACATTTTAAATTTGTCAA‑ACCTTTTTGCAGATTAAGCCAAAATAACCCTTCGGCAAAG‑CCTCAGT‑TACGCGGAGAgggaggg                                                        >  1:453002/1‑146 (MQ=255)
                                                                                          ttAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATgg                                                        <  1:256392/150‑1 (MQ=255)
                                                                                            aGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑CGCCTT‑TTTAACAATTATAATTGATTAA‑ACCTTCTGGCAGATTAAGCCAAAAAAAACGGTCGGGAATG‑CTTTAAT‑TAAGCGAAGAgggaagggct                                                     >  1:177904/1‑143 (MQ=255)
                                                                                              aCAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑ATTTTGCTT‑CCCCTATTTAAACATTT‑GAAATTATCCA‑TCCTTCCGGCGGATTATGACAAAAAACCCGTTACGCAACG‑CCTACAT‑TTCGCGAaaaggaaagg                                                        >  1:891271/1‑146 (MQ=255)
                                                                                                     ccAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGTCG‑GGCTTA‑TTTTACAATTTTAATTTATCAA‑ATCTTTTGGGCGATTTAGCCCAAAAACCCCGTCGGGACTG‑CTTTATT‑TTAGGGCAGAGGGATGGGGTTAATTCttt                                            >  1:1056151/1‑151 (MQ=255)
                                                                                                     ccAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCttt                                            <  1:1113051/151‑1 (MQ=255)
                                                                                                          cAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCTG‑CGCTTA‑TTTAACATTTTGAATTGATCAA‑TCCTTCTGGCAGATTAAACCAAAAAAACCGTTCGGCCATG‑CTTCCGT‑TACGCGGAGAGGGATGGTCTTAAtttttttttt                                        >  2:1119816/1‑150 (MQ=255)
                                                                                                          cAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCttttttt                                        <  1:1078046/150‑1 (MQ=255)
                                                                                                          cAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTg                                       <  1:252931/151‑1 (MQ=255)
                                                                                                           aGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTTGCCG‑GGCCTA‑TTTAAAATTTTGAATTGGTCAA‑ACCTTCTTGCAGATTAAGCCAAAAAAACCCTTCGGCAATG‑CTTTAGT‑TACGCGGAGAGGGATGGTCTGAAtttttttttt                                        >  2:179418/1‑149 (MQ=255)
                                                                                                             gCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGtt                                    <  1:199107/151‑1 (MQ=255)
                                                                                                             gCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGtt                                    <  1:460245/151‑1 (MQ=255)
                                                                                                               gTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTg                                   <  1:896933/150‑1 (MQ=255)
                                                                                                                 tAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCt                                 <  1:692728/150‑1 (MQ=255)
                                                                                                                  aaaaaTGGGGCTGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTTAACATTTAGAATTGGTCAA‑TTCTTTTTGCAGATTAAACCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TTCGCGAAGAGGGATGGTCTGAATTCTTTTTTTTGTTGCt                                 >  2:685947/1‑149 (MQ=255)
                                                                                                                   aaaaTGGGGCGGATAGTGAGGCGG‑TTTTTGGCG‑GGCCTA‑TTTAACAATTAGAAATGATTAA‑TCCTTTTCGGAGAATAAGACAAAAAAACCGTTCGGCAATGCCTCCAGTATCCGC‑AAGGGGAA‑GGGCTGAAtttttttttttttttctta                               >  1:1010505/1‑141 (MQ=255)
                                                                                                                   aaaaTGGGGCGGATAGTGAGGCGG‑TTTTTGCCT‑CGCTTC‑TTGAAAATTTAGAATTGATCAA‑ACCTTCTGGCAGATTAAGCCAAAATAACCGTTCGGCCATG‑CTTCAGG‑TTCGCGAAGAGGGATGGTCTGAAATCttttttttggt                                    >  2:926463/1‑141 (MQ=255)
                                                                                                                    aaaTGGGGCGGATAGTGAGGCGG‑TTTTTGGTG‑CGCTTA‑TTTAACATTTTGAAATGGTCAA‑TTCTTTTGGCAGATTAAACCAAAATAACCGTTTGGCAATG‑CTTTAGT‑TACGCGGAGAGGGATGGTCTGAATTTTTTTTTTGGTTGCTaaa                              >  2:629771/1‑150 (MQ=255)
                                                                                                                           gCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTgg                      <  1:751428/151‑1 (MQ=255)
                                                                                                                             ggATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAg                    <  1:54112/151‑1 (MQ=255)
                                                                                                                                 aGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAgtgt                 <  1:293620/150‑1 (MQ=255)
                                                                                                                                  gTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTc                >  2:64148/1‑150 (MQ=255)
                                                                                                                                    gAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTg              <  1:956708/150‑1 (MQ=255)
                                                                                                                                    gAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTg              <  1:136682/150‑1 (MQ=255)
                                                                                                                                      ggCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGAt           <  2:782295/151‑1 (MQ=255)
                                                                                                                                         gg‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAt        <  1:1074514/151‑1 (MQ=255)
                                                                                                                                              ttGGCTG‑CGCTTA‑TTGGACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTAtgtg     <  2:369696/150‑1 (MQ=255)
                                                                                                                                                ggCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGatt  >  1:810373/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                           
ATCCGATAAAGTCGGTTCCCGGTAACAACAACAGAATCCTTTTTTAATTATTGTTTCGTTGTTTTTGGTGTGATTCAGAAAATAAATATTTTAGACAGTAGCCAGTCAGGCGTTAAAAATGGGGCGGATAGTGAGGCGG‑TTTTGGCTG‑CGCTTA‑TTGAACATTTAGAATTGATCAA‑TCCTTCTGGCAGATTAAGCCAAAATACCCGTTCGGCAATG‑CTTCAGT‑TACGCGAAGAGGAATGGTCTGAATTCTTTTTTTGGTTGCTAAATTCTCTGGAGTGTCTGGATTATGTGATT  >  NC_000913/1245091‑1245384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑