Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 82,372 T→C E386E (GAA→GAG leuA ← 2‑isopropylmalate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091382,3720TC98.8% 310.4 / ‑4.4 80E386E (GAA→GAGleuA2‑isopropylmalate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (31/48);  total (31/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.97e-01

TGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATG  >  NC_000913/82225‑82515
                                                                                                                                                   |                                                                                                                                               
tGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGctcctc                                                                                                                                               >  1:659675/1‑150 (MQ=255)
 gCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTcctctt                                                                                                                                             >  1:311446/1‑151 (MQ=255)
 gCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTcctctt                                                                                                                                             >  1:260811/1‑151 (MQ=255)
    tGATCGATGGCATCGCCCGGCGCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTCCGCCACAGGCCAGTTTGACGGCGGCGGCTGCCTTATCGATAGAGTCAACCACCACGCTGAAGTCATTCAGACGGAAGTCTTCCGGctcctcat                                                                                                                                             >  1:109708/1‑146 (MQ=255)
    tGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTtgct                                                                                                                                          >  1:567535/1‑151 (MQ=255)
     gATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCGCTtgctg                                                                                                                                         <  2:744274/151‑1 (MQ=255)
       tAGACGGCATCAACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTgctgct                                                                                                                                       >  2:611112/1‑151 (MQ=255)
              cATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGAt                                                                                                                                >  2:480084/1‑151 (MQ=255)
                tCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATg                                                                                                                               >  1:62822/1‑150 (MQ=255)
                 cGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGa                                                                                                                              >  2:707308/1‑150 (MQ=255)
                     cggaccgTTACCGTTGGCGGCTTCTGCTTTTACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGcc                                                                                                                         <  2:659698/151‑1 (MQ=255)
                      ggaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCa                                                                                                                        <  1:275803/151‑1 (MQ=255)
                       gaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAg                                                                                                                       <  2:804868/151‑1 (MQ=255)
                       gaccgTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGGGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGcc                                                                                                                         >  1:85796/1‑149 (MQ=255)
                         ccgttaccgttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAgcg                                                                                                                     >  2:1168990/1‑151 (MQ=255)
                           gttaccgttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGcc                                                                                                                   <  2:76654/151‑1 (MQ=255)
                           gttaccgttGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGcc                                                                                                                   >  2:409702/1‑151 (MQ=255)
                                   tGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGGGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTcc                                                                                                                >  2:1220833/1‑146 (MQ=255)
                                    ggcggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCg                                                                                                          <  1:1176291/151‑1 (MQ=255)
                                     gcggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGt                                                                                                         <  2:439327/151‑1 (MQ=255)
                                      cggcTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTa                                                                                                        <  2:180382/151‑1 (MQ=255)
                                         cTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATc                                                                                                     >  1:708252/1‑151 (MQ=255)
                                           tCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCTAGATCGTAATCa                                                                                                    >  2:128485/1‑150 (MQ=255)
                                           tCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATc                                                                                                     <  2:646154/149‑1 (MQ=255)
                                               cTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCaaa                                                                                                  <  2:206812/148‑1 (MQ=255)
                                                tttGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACAcc                                                                                              >  2:878945/1‑151 (MQ=255)
                                                  tGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTg                                                                                            >  2:887744/1‑151 (MQ=255)
                                                      ttcttcGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCt                                                                                        >  1:1151203/1‑151 (MQ=255)
                                                          tcGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCttt                                                                                      <  2:739554/149‑1 (MQ=255)
                                                             ccACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGt                                                                                 <  2:806347/151‑1 (MQ=255)
                                                             ccACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGt                                                                                 <  2:1207827/151‑1 (MQ=255)
                                                                caGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCg                                                                              <  1:972006/151‑1 (MQ=255)
                                                                caGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCg                                                                              >  2:761985/1‑151 (MQ=255)
                                                                 aGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGc                                                                             >  2:938172/1‑151 (MQ=255)
                                                                 aGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGc                                                                             >  1:332963/1‑151 (MQ=255)
                                                                  ggCCGGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGGTTGCACGCTGAGGTAGTCCAGACGGAAAGGCTGCGGCTACTCTTGCTGCTTACCGGTGGGGGCCAGCGCCTCCAGATCGTAATCAAACACCTGTGCTTTTTTGTCCg                                                                              <  1:778788/149‑1 (MQ=255)
                                                                   gCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGcc                                                                            >  2:586747/1‑150 (MQ=255)
                                                                     cAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGc                                                                         <  2:1179805/151‑1 (MQ=255)
                                                                     cAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGc                                                                         <  1:1114655/151‑1 (MQ=255)
                                                                      aGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCt                                                                        <  2:985424/151‑1 (MQ=255)
                                                                         ttGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTc                                                                      <  1:714588/150‑1 (MQ=255)
                                                                         ttGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTc                                                                      <  1:316806/150‑1 (MQ=255)
                                                                           gACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAg                                                                    <  2:590867/150‑1 (MQ=255)
                                                                                cggcggTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGc                                                              <  2:356220/151‑1 (MQ=255)
                                                                                          gATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACaaa                                                    <  1:1126032/151‑1 (MQ=255)
                                                                                            tatCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAAtt                                                  >  1:139907/1‑151 (MQ=255)
                                                                                               cGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGt                                                <  1:611094/150‑1 (MQ=255)
                                                                                               cGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTc                                               <  2:622624/151‑1 (MQ=255)
                                                                                                  tAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTa                                             <  2:818413/150‑1 (MQ=255)
                                                                                                   agagCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTaaa                                           <  1:1092791/151‑1 (MQ=255)
                                                                                                   agagCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTaaa                                           <  1:899597/151‑1 (MQ=255)
                                                                                                      gCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAAtt                                         >  1:541023/1‑150 (MQ=255)
                                                                                                      gCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATta                                        <  2:868035/151‑1 (MQ=255)
                                                                                                       ccAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATtat                                       <  1:717489/151‑1 (MQ=255)
                                                                                                       ccAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATtat                                       <  1:997197/151‑1 (MQ=255)
                                                                                                         aGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTatat                                     >  1:260063/1‑151 (MQ=255)
                                                                                                           aCTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTc                                   <  1:701068/151‑1 (MQ=255)
                                                                                                           aCTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTc                                   <  1:1125917/151‑1 (MQ=255)
                                                                                                              gCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCa                                  <  1:601855/149‑1 (MQ=255)
                                                                                                               cACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActt                               >  2:1017069/1‑151 (MQ=255)
                                                                                                                  gCTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttct                            <  2:1026375/151‑1 (MQ=255)
                                                                                                                   cTGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttctt                           >  2:1198415/1‑151 (MQ=255)
                                                                                                                    tGAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttcttt                          >  2:1174656/1‑151 (MQ=255)
                                                                                                                     gAAGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttcttt                          >  2:1138726/1‑150 (MQ=255)
                                                                                                                      aaGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCActttcttt                          <  1:376149/149‑1 (MQ=255)
                                                                                                                      aaGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTtat                        <  1:598039/151‑1 (MQ=255)
                                                                                                                       aGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGTCCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTtata                       <  1:477810/151‑1 (MQ=255)
                                                                                                                       aGTAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTtata                       <  2:973932/151‑1 (MQ=255)
                                                                                                                         tAATCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAcc                     >  1:1087290/1‑151 (MQ=255)
                                                                                                                          aaTCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAcc                     <  1:1205509/150‑1 (MQ=255)
                                                                                                                           aTCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCGAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAcccc                   >  1:247696/1‑151 (MQ=255)
                                                                                                                           aTCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAcccc                   <  2:1105697/151‑1 (MQ=255)
                                                                                                                           aTCCAGACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATAcccc                   >  1:1030916/1‑151 (MQ=255)
                                                                                                                                gACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAtctc              >  2:45580/1‑151 (MQ=255)
                                                                                                                                gACGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCAtctc              <  1:1220766/151‑1 (MQ=255)
                                                                                                                                  cGGAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCa             <  1:280162/150‑1 (MQ=255)
                                                                                                                                   ggAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATc           <  1:586730/151‑1 (MQ=255)
                                                                                                                                   ggAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATc           <  2:253243/151‑1 (MQ=255)
                                                                                                                                   ggAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGAACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATc           >  1:96490/1‑151 (MQ=255)
                                                                                                                                    gAAATGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATcc          <  2:508141/151‑1 (MQ=255)
                                                                                                                                      aaTGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCAt        <  2:723760/151‑1 (MQ=255)
                                                                                                                                      aaTGCTCCGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCAt        <  2:429205/151‑1 (MQ=255)
                                                                                                                                            ccGGCTCCTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATg  >  2:732516/1‑151 (MQ=255)
                                                                                                                                              ggCTCCTCTTGCTGCTTACCGATGGAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATg  <  2:1000446/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TGCCTGATAGACGGCATCGACCGGACCGTTACCGTTGGCGGCTTCTGCTTTGACTTCTTCGCCACAGGCCAGTTTGACGGCGGCGGTGGCGATATCGTTAGAGCCAGACTGCACGCTGAAGTAATCCAGACGGAAATGCTCCGGCTCTTCTTGCTGCTTACCGATGAAGGCCAGCGCCTCCAGATCGTAATCAAACACCTGACCTTTTTTGTCCGCCAGCTTCAGGAAAGCATCGTACAAATTGTCTAAATTATATTCACTTTCTTTATACCCCATCTCATCCATGCGATG  >  NC_000913/82225‑82515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: