| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,509,609 | A→C | intergenic (‑183/‑21) | glk ← / → yfeO | glucokinase/putative ion channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,509,609 | 0 | A | C | 98.2% | 207.6 / ‑4.2 | 55 | intergenic (‑183/‑21) | glk/yfeO | glucokinase/putative ion channel protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (24/30); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
CTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCT > NC_000913/2509463‑2509751 | ctcACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCcccc > 1:415422/1‑151 (MQ=255) tcACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCg > 2:1196664/1‑151 (MQ=255) cacaCTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGcccc < 2:24079/148‑1 (MQ=255) cacaCTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCcccc < 1:1080801/149‑1 (MQ=255) cacaCTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCcccc < 2:323745/149‑1 (MQ=255) acacTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGc > 1:1177386/1‑150 (MQ=255) tGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATa > 2:202430/1‑149 (MQ=255) gTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATaa < 1:616360/149‑1 (MQ=255) gTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAg < 2:302871/150‑1 (MQ=255) aaTACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAAt > 2:2198/1‑151 (MQ=255) tACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAAt > 1:797331/1‑149 (MQ=255) ccTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGaa < 2:2887/151‑1 (MQ=255) gggggCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAg < 1:1046304/146‑1 (MQ=255) gggggCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACa < 2:544195/150‑1 (MQ=255) ggggCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATg < 1:534401/151‑1 (MQ=255) aTAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCt > 2:954884/1‑148 (MQ=255) aacaacaacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCAt < 2:773496/150‑1 (MQ=255) aacGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCcgcg > 2:147620/1‑149 (MQ=255) acGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGc < 2:132874/151‑1 (MQ=255) gCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCa < 2:767297/151‑1 (MQ=255) gCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCa < 1:903277/151‑1 (MQ=255) ccGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGcc > 1:610362/1‑149 (MQ=255) ccGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGcc > 1:373495/1‑149 (MQ=255) ccGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAg < 2:1190687/151‑1 (MQ=255) aggcaggcTCCCTATAAATATCGATCTGGGTCACTCAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCa < 1:752066/150‑1 (MQ=255) gcaggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAtgt < 1:423748/151‑1 (MQ=255) ggcTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCAtgttgt > 2:114698/1‑151 (MQ=255) gATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCccgcc > 1:1218315/1‑151 (MQ=255) aTCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCCGAACCATGTTGTTATTATCGCTCCccgccg > 2:673879/1‑151 (MQ=255) tCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGt > 2:537478/1‑151 (MQ=255) cTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTg > 2:1124183/1‑151 (MQ=255) cTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTg > 2:1137232/1‑151 (MQ=255) gggTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCccgcc < 1:296599/146‑1 (MQ=255) aTCGTTTCAGCCCCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCACCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCa < 2:53710/150‑1 (MQ=255) ttCAGCACCAATTGCAGCGATGCCTTTTGGAAAGCTGGGAAAACTAAGTATCTGCCCCCGAATAAGAAATAGAACATGCTCCACCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCtt < 2:790841/150‑1 (MQ=255) cACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGAAAACTAAGTACCTGCCCCCGCATAAGGAATAGAACATGCCCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCt < 2:1059935/150‑1 (MQ=255) aCCAATTGCAGCGATGCCTTTTTGAAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGCCCAGTCTTATTCTg > 1:1035277/1‑150 (MQ=255) aaTTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATtg > 1:374793/1‑151 (MQ=255) aaTTGCAGCGATCCTTTTTTGCAAGCTGGGAAAACTAAGTATCTGCCCCCGCATAAGGAATAAAACATGCTCCATCCGCGAGCAAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATtg < 2:867919/151‑1 (MQ=255) aTGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGtgatga > 1:459427/1‑150 (MQ=255) tGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGaa > 1:471135/1‑150 (MQ=255) ccTTTTTGCAAGCGGGGCAAACTAAGTATCTGCCCCCGAATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATTTTTTTTTTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGaaa < 1:1137165/149‑1 (MQ=255) ttttGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATc < 2:258688/149‑1 (MQ=255) ttGCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCt > 2:143324/1‑151 (MQ=255) tccaggCTGGGAAAACTAACTATCTGCCCCCGTATAAGGAATAGAACATGCTCCATCCGCGGGCCAGAACCTTGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCt < 2:24585/145‑1 (MQ=255) gCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCg > 1:915130/1‑151 (MQ=255) gCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCg > 1:442177/1‑151 (MQ=255) gCAAGCTGGGCAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCg > 1:680466/1‑151 (MQ=255) gCAAGCTGGGAAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCACCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGcc < 2:274463/148‑1 (MQ=255) tGGGAAAACTAAGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTAt < 2:511677/149‑1 (MQ=255) gggAAAACTAAGTATCTGCCCCCGCATAAGAAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTAtt < 2:780240/149‑1 (MQ=255) aaCTAATTAGCTCCCCCCGAATAAGAAATAAAACTTCCGCCTCCGGCGAGCCAGAACCATTTTGTTATTACCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGa < 2:518841/149‑1 (MQ=255) aggtttCTGCCCCCGCATAAGAAATAGAACGTGCCCCATCCGGGGGCCAGACCCTTTTTTTTTTTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTg < 1:177551/145‑1 (MQ=255) aaGTCTCTGCCCCCGCAAAAGGAATAGAACATGCTCCACCCGCGAGCCAGAACCATTTTGTTATTATCGCCCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTg < 1:1182549/150‑1 (MQ=255) aaGTATCTGCCCCCGCATAAGGAAAAAAACATGCTCCACCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTg < 2:23906/150‑1 (MQ=255) aGTATCTGCCCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGct > 2:464115/1‑151 (MQ=255) | CTCACACTGTAAATACCTGGGGGCATAACAACAACGCCGGAAAGGCAGGCTCCCTGTAAATATCGATCTGGGTCACACAATTACTTTATCGTTTCAGCACCAATTGCAGCGATGCCTTTTTGCAAGCTGGGCAAACTAAGTATCTGACCCCGCATAAGGAATAGAACATGCTCCATCCGCGAGCCAGAACCATGTTGTTATTATCGCTCCCCGCCGTGGCAATTGGGATTGCGTCCAGTCTTATTCTGATTGTGGTGATGAAAATCGCCTCGGTATTACAGAATTTGCT > NC_000913/2509463‑2509751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |