Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,617	G→A	L186L (CTG→CTA)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,617	0	G	A	100.0%	262.8 / NA	69	L186L (CTG→CTA)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/31);  total (38/31)

TTCTGATTCCGGT‑AAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTGAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAA  >  NC_000913/3016473‑3016764                                                                                                                                                   |                                                                                                                                                    tttggttcccGGT‑AAACCTTTAATTTTAAATATGCGTGCCGTTTACACCCCGGAAGTTATAAAGA‑ACCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATa                                                                                                                                                 <  2:609102/141‑1 (MQ=255)  ttctaTTCCGGGAAAATCTTTTATTTTAAATGGGAGCGTCGGTACCACCCTGGAAGTTATAAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATaa                                                                                                                                                <  1:1037414/146‑1 (MQ=255)   cTGATTCCGGT‑AAATCGTTCATCTTTAATATGAGCGACGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAAtt                                                                                                                                             >  2:1172016/1‑151 (MQ=255)     gATTCCGGT‑AAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTAc                                                                                                                                           >  2:820012/1‑151 (MQ=255)      aTTCCGGT‑AAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACt                                                                                                                                          >  2:917526/1‑151 (MQ=255)            gT‑AAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAgg                                                                                                                                     >  1:511135/1‑150 (MQ=255)            gT‑AAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAgg                                                                                                                                     >  2:272163/1‑150 (MQ=255)                      tCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAtt                                                                                                                           >  2:709776/1‑151 (MQ=255)                        ttttaaaaTAGGAGCGTCGCTAACACCCCGGAGGTTATTAAGA‑ACCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCa                                                                                                                                       <  2:151288/131‑1 (MQ=255)                         tCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAttttt                                                                                                                        >  2:836754/1‑151 (MQ=255)                          cTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCAttttt                                                                                                                        >  1:976015/1‑150 (MQ=255)                            ttAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGc                                                                                                                     >  1:174084/1‑151 (MQ=255)                             tAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCt                                                                                                                    >  2:797208/1‑151 (MQ=255)                              aaTATGGGCGTTGTTAACACCCTGGAGGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTc                                                                                                                   <  2:691071/151‑1 (MQ=255)                              aaTATGGGCGTCTTTAACAACCGAAAAGGTATTAACC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTa                                                                                                                       <  1:355408/147‑1 (MQ=255)                              aaTATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTc                                                                                                                   >  2:154960/1‑151 (MQ=255)                              aaTATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTc                                                                                                                   >  2:847368/1‑151 (MQ=255)                              aaTATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCCAAATAAATTACTCCAGGATGACGCATTTTTAGCTc                                                                                                                   >  2:514481/1‑151 (MQ=255)                               atatGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCg                                                                                                                  >  2:972001/1‑151 (MQ=255)                                   gAGCGTCTTTAACACCCCGGAAGTTATAAACC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGcc                                                                                                                <  1:792217/149‑1 (MQ=255)                                      cGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACgg                                                                                                            >  1:658967/1‑150 (MQ=255)                                         cGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTg                                                                                                        >  2:1214613/1‑151 (MQ=255)                                           ggtgcaacccaagaGGTATAAAGCGATCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTg                                                                                                        <  1:923815/137‑1 (MQ=255)                                             accacccaagAAGCTATAAAGC‑ACCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGAt                                                                                                          <  1:314493/136‑1 (MQ=255)                                              aCAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAgg                                                                                                    >  1:408788/1‑150 (MQ=255)                                                aaCCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGCTGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACACCAGGATGACGCATTCTTAGCTCGCCACGTATTGCAgg                                                                                                    >  1:71550/1‑148 (MQ=255)                                                    tCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAcg                                                                                             >  1:391907/1‑151 (MQ=255)                                                      gAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAcgcg                                                                                           >  2:832882/1‑151 (MQ=255)                                                      gAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAAcgcg                                                                                           >  1:238592/1‑151 (MQ=255)                                                          gTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGa                                                                                          >  2:554260/1‑148 (MQ=255)                                                                 gC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCa                                                                                 >  2:440545/1‑150 (MQ=255)                                                                  gaatccgccgATGCAACAGTGCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGc                                                                                      <  1:614932/141‑1 (MQ=255)                                                                    aaCCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGc                                                                              >  2:222715/1‑151 (MQ=255)                                                                       cgccgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCtt                                                                           <  2:333028/151‑1 (MQ=255)                                                                       cgccgATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCtt                                                                           >  1:628961/1‑151 (MQ=255)                                                                           gATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTAccc                                                                       >  2:319389/1‑151 (MQ=255)                                                                              gCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCt                                                                    <  2:1163004/151‑1 (MQ=255)                                                                               cAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTc                                                                   <  2:1193421/151‑1 (MQ=255)                                                                                aaCTGTTCATCCACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCTCGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTc                                                                   <  1:687708/150‑1 (MQ=255)                                                                                 aCAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATATATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGACTTACCCGCTCGc                                                                 >  1:932157/1‑151 (MQ=255)                                                                                   aGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCAt                                                               <  2:738622/151‑1 (MQ=255)                                                                                      tCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATcc                                                             <  1:1008351/150‑1 (MQ=255)                                                                                          cGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCccac                                                         <  2:20001/150‑1 (MQ=255)                                                                                           gACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTAGCCGCTCGCATCCccacca                                                       <  2:527533/151‑1 (MQ=255)                                                                                            aCAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGAGTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCcca                                                          <  2:916899/147‑1 (MQ=255)                                                                                                 atgatgGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATgg                                                 >  1:94990/1‑151 (MQ=255)                                                                                                 atgatgGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATg                                                  <  2:897342/150‑1 (MQ=255)                                                                                                       gACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATg                                           <  1:587462/151‑1 (MQ=255)                                                                                                        aCGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATgg                                          <  2:1049389/151‑1 (MQ=255)                                                                                                         cGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGc                                         <  2:457428/151‑1 (MQ=255)                                                                                                         cGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGc                                         >  2:935383/1‑151 (MQ=255)                                                                                                          gCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCg                                        >  2:1104384/1‑151 (MQ=255)                                                                                                            aTCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGt                                       <  1:377685/150‑1 (MQ=255)                                                                                                                gACCATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTc                                      <  2:238889/147‑1 (MQ=255)                                                                                                                   cATCCGAAATTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCctct                               >  1:1204187/1‑151 (MQ=255)                                                                                                                          aaTTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCAt                        <  1:274597/151‑1 (MQ=255)                                                                                                                          aaTTCGCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCAt                        <  1:489781/151‑1 (MQ=255)                                                                                                                               gCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACg                   >  2:1010742/1‑151 (MQ=255)                                                                                                                               gCTCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACg                   <  1:238544/151‑1 (MQ=255)                                                                                                                                 tCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGc                 >  1:973746/1‑151 (MQ=255)                                                                                                                                 tCAATATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGc                 >  1:1164190/1‑151 (MQ=255)                                                                                                                                   aaTATCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTg               >  2:216306/1‑151 (MQ=255)                                                                                                                                      atCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtc             <  1:304487/150‑1 (MQ=255)                                                                                                                                       tCGCGATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtcct           >  1:528581/1‑151 (MQ=255)                                                                                                                                          cgATACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGtcctcc         <  1:917470/150‑1 (MQ=255)                                                                                                                                            aTACGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGc       >  2:691475/1‑150 (MQ=255)                                                                                                                                              aCGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCAt     <  2:511150/150‑1 (MQ=255)                                                                                                                                              aCGCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATg    >  1:395349/1‑151 (MQ=255)                                                                                                                                                gCTAAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGaa  <  1:558179/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TTCTGATTCCGGT‑AAATCGTTCATCTTTAATATGAGCGTCGGTTACAACCTCGAAGGTATTAAGC‑AACCGCCGATGCAACAGTTCATCGACAATATGATGGACGCATCTGACCATCCGAAATTCGCTCAATATCGCGATACGCTGAATAAATTACTCCAGGATGACGCATTTTTAGCTCGCCACGGATTGCAGGAAAAACGCGAAAGCTTGCAAGCCTTACCCGCTCGCATCCCCACCAGTATGGTGCATGGCGTCACCCTCTCCACCATGCACGGCTGTCCTCCGCATGAA  >  NC_000913/3016473‑3016764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑