Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,436,622 T→C R35R (CGT→CGC trkA → NAD‑binding component of TrK potassium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,436,6220TC98.9% 308.5 / ‑4.0 88R35R (CGT→CGCtrkANAD‑binding component of TrK potassium transporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (45/42);  total (46/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

CGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCC  >  NC_000913/3436475‑3436767
                                                                                                                                                   |                                                                                                                                                 
cGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCt                                                                                                                                                 >  2:154552/1‑150 (MQ=255)
cGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTg                                                                                                                                                >  1:989403/1‑151 (MQ=255)
 gctaagctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGc                                                                                                                                               <  1:24852/151‑1 (MQ=255)
  ctaagctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCg                                                                                                                                              >  2:765107/1‑151 (MQ=255)
  ctaagctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCg                                                                                                                                              >  2:526087/1‑151 (MQ=255)
    aagctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGa                                                                                                                                            <  2:398712/151‑1 (MQ=255)
       ctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCt                                                                                                                                         >  2:655674/1‑151 (MQ=255)
       ctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCt                                                                                                                                         >  2:516129/1‑151 (MQ=255)
         aaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCtt                                                                                                                                        <  1:472143/150‑1 (MQ=255)
         aaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCt                                                                                                                                         <  1:337512/149‑1 (MQ=255)
           tCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTAc                                                                                                                                      >  1:851905/1‑150 (MQ=255)
            cAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTAc                                                                                                                                      >  1:733459/1‑149 (MQ=255)
             aaaaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAgg                                                                                                                                   <  1:125634/151‑1 (MQ=255)
             aaaaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAgg                                                                                                                                   >  1:65011/1‑151 (MQ=255)
             aaaaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAgg                                                                                                                                   <  2:42993/151‑1 (MQ=255)
              aaaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAgg                                                                                                                                   >  2:622889/1‑150 (MQ=255)
               aaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGAt                                                                                                                                 <  1:598398/151‑1 (MQ=255)
               aaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGAt                                                                                                                                 >  2:1112843/1‑151 (MQ=255)
                aaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATa                                                                                                                                <  1:1166897/151‑1 (MQ=255)
                  gTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCAGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATaa                                                                                                                               <  1:181900/150‑1 (MQ=255)
                           tAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGTGTCTGCGGACCTTACAGGATAAATTTGACCTg                                                                                                                     >  1:248732/1‑151 (MQ=255)
                           tAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTg                                                                                                                     >  1:385807/1‑151 (MQ=255)
                            aaCGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTg                                                                                                                     <  2:755722/150‑1 (MQ=255)
                            aaCGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGc                                                                                                                    >  1:1059906/1‑151 (MQ=255)
                                ggTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTTTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCg                                                                                                                   >  1:1081714/1‑148 (MQ=255)
                                 gTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTc                                                                                                               <  1:171963/151‑1 (MQ=255)
                                   cgcgACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCg                                                                                                              <  1:902919/150‑1 (MQ=255)
                                    gcgACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGAGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTg                                                                                                            <  2:737122/151‑1 (MQ=255)
                                      gACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCa                                                                                                          <  1:202014/151‑1 (MQ=255)
                                       aCTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGc                                                                                                           >  1:915001/1‑149 (MQ=255)
                                         tgatgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAggg                                                                                                       >  2:472780/1‑151 (MQ=255)
                                         tgatgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAggg                                                                                                       >  2:56554/1‑151 (MQ=255)
                                           atgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGc                                                                                                     <  2:402931/151‑1 (MQ=255)
                                            tgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGc                                                                                                     >  2:391244/1‑150 (MQ=255)
                                                aaTTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGc                                                                                                >  2:64958/1‑151 (MQ=255)
                                                   tATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGctc                                                                                              <  1:567487/150‑1 (MQ=255)
                                                    aTCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGctct                                                                                             <  2:305021/150‑1 (MQ=255)
                                                            gggTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcgc                                                                                    >  2:1095377/1‑151 (MQ=255)
                                                            gggTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCAcg                                                                                     <  2:137467/150‑1 (MQ=255)
                                                                  cGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTg                                                                              >  1:1053261/1‑151 (MQ=255)
                                                                   ggCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGc                                                                             >  2:57037/1‑151 (MQ=255)
                                                                   ggCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGc                                                                             >  2:866610/1‑151 (MQ=255)
                                                                     ccAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCgg                                                                           <  2:1015798/151‑1 (MQ=255)
                                                                      cAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCggg                                                                          >  1:1124397/1‑151 (MQ=255)
                                                                       aGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCggg                                                                          >  1:627974/1‑150 (MQ=255)
                                                                       aGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGa                                                                         <  2:1145321/151‑1 (MQ=255)
                                                                        ggTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCggg                                                                          >  1:35721/1‑149 (MQ=255)
                                                                         gTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAgg                                                                       >  1:568959/1‑151 (MQ=255)
                                                                           tGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGc                                                                      >  1:650722/1‑150 (MQ=255)
                                                                           tGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGc                                                                      <  1:631355/150‑1 (MQ=255)
                                                                            ggcggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCa                                                                     <  1:348680/150‑1 (MQ=255)
                                                                              cggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGt                                                                  >  2:896381/1‑151 (MQ=255)
                                                                              cggcACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGt                                                                  <  1:352091/151‑1 (MQ=255)
                                                                                  acacTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCcg                                                              >  2:32197/1‑151 (MQ=255)
                                                                                       ggCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCgacgac                                                         >  2:350216/1‑151 (MQ=255)
                                                                                        gCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACCGCGAGCGCCTGCGGACCTTACAGGATCAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGAAGGTGCCgatgac                                                         >  1:285229/1‑150 (MQ=255)
                                                                                                  tGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCt                                              >  1:477598/1‑151 (MQ=255)
                                                                                                  tGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCt                                              >  2:80774/1‑151 (MQ=255)
                                                                                                      tGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGtt                                          >  2:180881/1‑151 (MQ=255)
                                                                                                      tGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGt                                           <  1:20709/150‑1 (MQ=255)
                                                                                                       ggCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTg                                         >  2:1015419/1‑151 (MQ=255)
                                                                                                           agaACAACGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGt                                     <  1:889737/151‑1 (MQ=255)
                                                                                                               caacGATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAAcc                                 <  1:974926/151‑1 (MQ=255)
                                                                                                                   gATATTACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGtt                             >  1:622174/1‑151 (MQ=255)
                                                                                                                       ttACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTAGTTGCTGTAACCAGTTc                            >  2:586101/1‑148 (MQ=255)
                                                                                                                        tACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGa                         <  1:276800/150‑1 (MQ=255)
                                                                                                                        tACTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGATCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTc                            >  1:1179945/1‑147 (MQ=255)
                                                                                                                         aCTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGAt                        <  1:1129757/150‑1 (MQ=255)
                                                                                                                         aCTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGAt                        <  1:618006/150‑1 (MQ=255)
                                                                                                                         aCTGTTGTCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGAt                        <  1:840290/150‑1 (MQ=255)
                                                                                                                           tgttgtCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGa                      >  2:79925/1‑150 (MQ=255)
                                                                                                                           tgttgtCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGa                      >  2:608659/1‑150 (MQ=255)
                                                                                                                            gttgtCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGaa                     <  1:1135859/150‑1 (MQ=255)
                                                                                                                             ttgtCGATACCAACGGTGAGCGCCTGCCGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGaa                     <  1:1132226/149‑1 (MQ=255)
                                                                                                                              tgtCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAAc                   <  1:1105155/150‑1 (MQ=255)
                                                                                                                               gtCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAAcc                  >  1:723869/1‑150 (MQ=255)
                                                                                                                               gtCGATACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAAcc                  <  2:332378/150‑1 (MQ=255)
                                                                                                                               gtCGATACCAACGGTGAGCGCCTGCGAACCTTACAGCATAAAGATGACCGGCGGGTGGTGCAGAGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGGCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCa                 <  1:390380/151‑1 (MQ=255)
                                                                                                                                    tACCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATg            >  1:675642/1‑151 (MQ=255)
                                                                                                                                     aCCAACGGTGAGCGCCTGCGGACCTTACATGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATg            <  2:672343/150‑1 (MQ=255)
                                                                                                                                     aCCAACGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATgg           <  2:1177454/151‑1 (MQ=255)
                                                                                                                                        aaCGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtg        >  2:477255/1‑151 (MQ=255)
                                                                                                                                          cGGTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcc      <  1:988629/151‑1 (MQ=255)
                                                                                                                                           ggTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcct     <  2:568976/151‑1 (MQ=255)
                                                                                                                                           ggTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcct     <  1:1126366/151‑1 (MQ=255)
                                                                                                                                            gTGAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctg    <  1:850077/151‑1 (MQ=255)
                                                                                                                                              gAGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctgc   <  1:486688/150‑1 (MQ=255)
                                                                                                                                               aGCGCCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctgcc  >  1:383196/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCC  >  NC_000913/3436475‑3436767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: